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Protein

Glutathione gamma-glutamylcysteinyltransferase 1

Gene

PCS1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the synthesis of phytochelatins (PC) and homophytochelatins (hPC), the heavy-metal-binding peptides of plants. Also involved in glutathione-conjugates degradation.5 Publications

Catalytic activityi

Glutathione + (Glu(-Cys))(n)-Gly = Gly + (Glu(-Cys))(n+1)-Gly.PROSITE-ProRule annotation

Enzyme regulationi

Requires cadmium for activity. Also activated in vitro or in heterologous system by Ag+, Hg+, Zn2+, Cu2+, Fe2+ or Fe3+ ions, but not by Co2+ or Ni2+ ions.3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei561
Active sitei1621
Active sitei1801

GO - Molecular functioni

  • ATPase-coupled arsenite transmembrane transporter activity Source: TAIR
  • cadmium ion binding Source: TAIR
  • copper ion binding Source: TAIR
  • glutathione gamma-glutamylcysteinyltransferase activity Source: TAIR
  • phytochelatin transmembrane transporter activity Source: TAIR

GO - Biological processi

  • arsenite transport Source: TAIR
  • defense response by callose deposition in cell wall Source: TAIR
  • defense response to bacterium Source: TAIR
  • indole glucosinolate catabolic process Source: TAIR
  • phytochelatin biosynthetic process Source: TAIR
  • response to arsenic-containing substance Source: TAIR
  • response to cadmium ion Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Ligandi

Cadmium, Copper, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciARA:AT5G44070-MONOMER.
MetaCyc:AT5G44070-MONOMER.
BRENDAi2.3.2.15. 399.
SABIO-RKQ9S7Z3.

Protein family/group databases

MEROPSiC83.002.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione gamma-glutamylcysteinyltransferase 1 (EC:2.3.2.15)
Alternative name(s):
Cadmium tolerance protein
Phytochelatin synthase 1
Short name:
AtPCS1
Gene namesi
Name:PCS1
Synonyms:ARA8, CAD1
Ordered Locus Names:At5g44070
ORF Names:MRH10.11, MRH10_18
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G44070.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: TAIR
Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Plants are highly sensitive to Cd (20- to 40-fold) and AsO43- ions, sensitive (eightfold) to Ag ions, slightly sensitive (twofold) to Cu and Hg ions, and insensitive to Zn, SeO32- and Ni ions.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi7Y → A: No effect. 1 Publication1
Mutagenesisi21S → A: No effect. 2 Publications1
Mutagenesisi56C → S or A: Loss of function. 2 Publications1
Mutagenesisi59A → V in cad1-4; loss of function. 1 Publication1
Mutagenesisi71D → A: Decreased activity. 1 Publication1
Mutagenesisi84D → A: No effect. 1 Publication1
Mutagenesisi89D → A: Slightly decreased activity. 1 Publication1
Mutagenesisi90C → S or A: Decreased activity. 2 Publications1
Mutagenesisi91C → S or A: No effect. 3 Publications1
Mutagenesisi91C → Y in cad1-1; loss of function. 3 Publications1
Mutagenesisi109C → S or A: No effect. 2 Publications1
Mutagenesisi113C → S or A: No effect. 2 Publications1
Mutagenesisi158T → A: No effect. 1 Publication1
Mutagenesisi162H → A: Loss of function. 1 Publication1
Mutagenesisi164S → A: No effect. 2 Publications1
Mutagenesisi174D → A: Decreased activity. 1 Publication1
Mutagenesisi180D → A: Loss of function. 1 Publication1
Mutagenesisi186Y → A: No effect. 1 Publication1
Mutagenesisi189H → A: Decreased activity. 1 Publication1
Mutagenesisi190W → C in cad1-3; loss of function. 1 Publication1
Mutagenesisi204D → A: Decreased activity. 1 Publication1
Mutagenesisi220H → A: Decreased activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002872101 – 485Glutathione gamma-glutamylcysteinyltransferase 1Add BLAST485

Proteomic databases

PaxDbiQ9S7Z3.
PRIDEiQ9S7Z3.

PTM databases

iPTMnetiQ9S7Z3.

Expressioni

Tissue specificityi

Expressed in roots and shoots.2 Publications

Developmental stagei

Expressed constitutively.1 Publication

Inductioni

Induced by copper, and by cadmium during the early stages of plant development. Gradual decrease of cadmium induction as plants continue to grow, and by 15 day after germination, total loss of induction.2 Publications

Gene expression databases

GenevisibleiQ9S7Z3. AT.

Interactioni

Protein-protein interaction databases

BioGridi19680. 3 interactors.
IntActiQ9S7Z3. 1 interactor.
STRINGi3702.AT5G44070.1.

Structurei

3D structure databases

ProteinModelPortaliQ9S7Z3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 221Peptidase C83PROSITE-ProRule annotationAdd BLAST221

Domaini

The intermediate part (284-372) is important for enzyme stabilization.
The C-terminal part (373-485) is required to determine enzyme responsiveness to a broad range of heavy metals.

Sequence similaritiesi

Belongs to the phytochelatin synthase family.PROSITE-ProRule annotation
Contains 1 peptidase C83 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0632. Eukaryota.
ENOG4111H5N. LUCA.
HOGENOMiHOG000241441.
InParanoidiQ9S7Z3.
KOiK05941.
OMAiTSSENCH.
OrthoDBiEOG09360CHS.
PhylomeDBiQ9S7Z3.

Family and domain databases

InterProiIPR007719. Phytochelatin_synthase.
IPR015407. Phytochelatin_synthase_C.
[Graphical view]
PfamiPF05023. Phytochelatin. 1 hit.
PF09328. Phytochelatin_C. 1 hit.
[Graphical view]
PROSITEiPS51443. PCS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9S7Z3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAMASLYRRS LPSPPAIDFS SAEGKLIFNE ALQKGTMEGF FRLISYFQTQ
60 70 80 90 100
SEPAYCGLAS LSVVLNALSI DPGRKWKGPW RWFDESMLDC CEPLEVVKEK
110 120 130 140 150
GISFGKVVCL AHCSGAKVEA FRTSQSTIDD FRKFVVKCTS SENCHMISTY
160 170 180 190 200
HRGVFKQTGT GHFSPIGGYN AERDMALILD VARFKYPPHW VPLKLLWEAM
210 220 230 240 250
DSIDQSTGKR RGFMLISRPH REPGLLYTLS CKDESWIEIA KYLKEDVPRL
260 270 280 290 300
VSSQHVDSVE KIISVVFKSL PSNFNQFIRW VAEIRITEDS NQNLSAEEKS
310 320 330 340 350
RLKLKQLVLK EVHETELFKH INKFLSTVGY EDSLTYAAAK ACCQGAEILS
360 370 380 390 400
GSPSKEFCCR ETCVKCIKGP DDSEGTVVTG VVVRDGNEQK VDLLVPSTQT
410 420 430 440 450
ECECGPEATY PAGNDVFTAL LLALPPQTWS GIKDQALMHE MKQLISMASL
460 470 480
PTLLQEEVLH LRRQLQLLKR CQENKEEDDL AAPAY
Length:485
Mass (Da):54,475
Last modified:May 1, 2000 - v1
Checksum:i27204B38310884D6
GO

Sequence cautioni

The sequence AAD29446 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti153G → S in AAD16046 (PubMed:10359847).Curated1
Sequence conflicti160T → N in AAD16046 (PubMed:10359847).Curated1
Sequence conflicti298E → A in AAL66747 (PubMed:12514032).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF135155 mRNA. Translation: AAD41794.1.
AF093753 mRNA. Translation: AAD50593.1.
AF085230 mRNA. Translation: AAD16046.1.
AF085231 Genomic DNA. Translation: AAD29446.1. Sequence problems.
AF162689 mRNA. Translation: AAF42805.1.
AF461180 mRNA. Translation: AAL66747.1.
AB006703 Genomic DNA. Translation: BAB09067.1.
CP002688 Genomic DNA. Translation: AED95055.1.
AY079384 mRNA. Translation: AAL85115.1.
AY048257 mRNA. Translation: AAK82519.1.
AY039951 mRNA. Translation: AAK64055.1.
RefSeqiNP_199220.1. NM_123774.4.
UniGeneiAt.7305.

Genome annotation databases

EnsemblPlantsiAT5G44070.1; AT5G44070.1; AT5G44070.
GeneIDi834430.
GrameneiAT5G44070.1; AT5G44070.1; AT5G44070.
KEGGiath:AT5G44070.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF135155 mRNA. Translation: AAD41794.1.
AF093753 mRNA. Translation: AAD50593.1.
AF085230 mRNA. Translation: AAD16046.1.
AF085231 Genomic DNA. Translation: AAD29446.1. Sequence problems.
AF162689 mRNA. Translation: AAF42805.1.
AF461180 mRNA. Translation: AAL66747.1.
AB006703 Genomic DNA. Translation: BAB09067.1.
CP002688 Genomic DNA. Translation: AED95055.1.
AY079384 mRNA. Translation: AAL85115.1.
AY048257 mRNA. Translation: AAK82519.1.
AY039951 mRNA. Translation: AAK64055.1.
RefSeqiNP_199220.1. NM_123774.4.
UniGeneiAt.7305.

3D structure databases

ProteinModelPortaliQ9S7Z3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi19680. 3 interactors.
IntActiQ9S7Z3. 1 interactor.
STRINGi3702.AT5G44070.1.

Protein family/group databases

MEROPSiC83.002.

PTM databases

iPTMnetiQ9S7Z3.

Proteomic databases

PaxDbiQ9S7Z3.
PRIDEiQ9S7Z3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G44070.1; AT5G44070.1; AT5G44070.
GeneIDi834430.
GrameneiAT5G44070.1; AT5G44070.1; AT5G44070.
KEGGiath:AT5G44070.

Organism-specific databases

TAIRiAT5G44070.

Phylogenomic databases

eggNOGiKOG0632. Eukaryota.
ENOG4111H5N. LUCA.
HOGENOMiHOG000241441.
InParanoidiQ9S7Z3.
KOiK05941.
OMAiTSSENCH.
OrthoDBiEOG09360CHS.
PhylomeDBiQ9S7Z3.

Enzyme and pathway databases

BioCyciARA:AT5G44070-MONOMER.
MetaCyc:AT5G44070-MONOMER.
BRENDAi2.3.2.15. 399.
SABIO-RKQ9S7Z3.

Miscellaneous databases

PROiQ9S7Z3.

Gene expression databases

GenevisibleiQ9S7Z3. AT.

Family and domain databases

InterProiIPR007719. Phytochelatin_synthase.
IPR015407. Phytochelatin_synthase_C.
[Graphical view]
PfamiPF05023. Phytochelatin. 1 hit.
PF09328. Phytochelatin_C. 1 hit.
[Graphical view]
PROSITEiPS51443. PCS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPCS1_ARATH
AccessioniPrimary (citable) accession number: Q9S7Z3
Secondary accession number(s): Q8W122
, Q9M6R0, Q9XHP8, Q9ZPM2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: May 1, 2000
Last modified: November 30, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Homoglutathione (hGSH) is a good acceptor, but a poor donor, of gamma-glutamylcysteine units.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.