Q9S7Z2 (AFP4_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ninja-family protein AFP4

Alternative name(s):
ABI five-binding protein 4
Short name=ABI5-binding protein 4
Protein TWO OR MORE ABRES-CONTAINING GENE 2

Gene names

Name:	AFP4
Synonyms:	TMAC2
Ordered Locus Names:	At3g02140
ORF Names:	F1C9.7, F14P3.21

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	319 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Acts as a negative regulator of abscisic acid (ABA) and salinity responses. Ref.4
Subunit structure	Interacts with ABI5/DPBF1, AREB3/DPBF3, EEL/DPBF4, ABF1 and ABF3/DPBF5. Ref.5
Subcellular location	Nucleus Ref.4.
Tissue specificity	Predominantly expressed in roots and seedlings. Ref.4
Induction	Up-regulated by abscisic acid (ABA) and salt. Down-regulated by cold and glucose. Ref.4 Ref.5
Disruption phenotype	Exhibits insensitivity to abscisic acid (ABA) and salt. Ref.5
Sequence similarities	Belongs to the Ninja family.

Ontologies

Keywords
Cellular component	Nucleus
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	negative regulation of abscisic acid mediated signaling pathway Inferred from mutant phenotype Ref.4. Source: TAIR regulation of starch biosynthetic process Inferred from mutant phenotype Ref.4. Source: TAIR response to abscisic acid stimulus Inferred from expression pattern Ref.4. Source: TAIR response to salt stress Inferred from expression pattern Ref.4. Source: TAIR
Cellular component	nucleus Inferred from direct assay Ref.4. Source: TAIR
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ABI5	Q9SJN0	2	EBI-1778843,EBI-1778690

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 319	319	Ninja-family protein AFP4		PRO_0000369617

Sequences

	Sequence		Length	Mass (Da)
	Q9S7Z2 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 275D25D6926FF5A0	FASTA	319	35,455
	10 20 30 40 50 60 MEMIRGRGIP NGVVQVPNLS ANNLLQKFFI ASNHFCQRDS EHGENQQEAK KREDEAEEDE 70 80 90 100 110 120 KDVELTLGLS LNGQFGTDPR SRKRRHFELG RSSSIPEGFV FDEQRSGGGN GGDMRRIVGR 130 140 150 160 170 180 GGSDMFQLDR TRSLPVVTEM DIEKERKVSE KTRAFMESPV TNRGAYLTKD KNRGQAVETE 190 200 210 220 230 240 KPRAFLEFKI PPTKEGKKEK DRLVVTGPVN GKGKNGNTAK KQKNNVENSG MEKARNILED 250 260 270 280 290 300 MPCVSTRDVG ADGKRVEGFL YWYGGNKEEV KIVCVCHGSF LSPAEFVRHG GGTVSDDDGG 310 DVMINPLRHI VVKLPSSSI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. Nature 408:820-822(2000) [PubMed: 11130713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Overexpression of TMAC2, a novel negative regulator of abscisic acid and salinity responses, has pleiotropic effects in Arabidopsis thaliana." Huang M.-D., Wu W.-L. Plant Mol. Biol. 63:557-569(2007) [PubMed: 17195036] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION.
[5]	"A small plant-specific protein family of ABI five binding proteins (AFPs) regulates stress response in germinating Arabidopsis seeds and seedlings." Garcia M.E., Lynch T.J., Peeters J., Snowden C., Finkelstein R.R. Plant Mol. Biol. 67:643-658(2008) [PubMed: 18484180] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE, SUBUNIT, INTERACTION WITH ABI5/DPBF1; AREB3/DPBF3; EEL/DPBF4; ABF1 AND ABF3/DPBF5, INDUCTION, DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC009755 Genomic DNA. Translation: AAF02123.1. AC011664 Genomic DNA. Translation: AAF14824.1. CP002686 Genomic DNA. Translation: AEE73768.1. AY034949 mRNA. Translation: AAK59455.1. AY062992 mRNA. Translation: AAL34166.1.
IPI	IPI00517712.
RefSeq	NP_566163.1. NM_111081.2.
UniGene	At.18540.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9S7Z2. 11 interactions.
STRING	Q9S7Z2.
Proteomic databases
PRIDE	Q9S7Z2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT3G02140.1; AT3G02140.1; AT3G02140.
GeneID	821182.
GenomeReviews	Gene locus AT3G02140 in contig BA000014_GR.
KEGG	ath:AT3G02140.
NMPDR	fig|3702.1.peg.12152.
Organism-specific databases
TAIR	At3g02140.
Phylogenomic databases
GeneTree	EPGT00050000001974.
HOGENOM	HBG747271.
OMA	RAFLEFK.
PhylomeDB	Q9S7Z2.
ProtClustDB	CLSN2917065.
Gene expression databases
ArrayExpress	Q9S7Z2.
Genevestigator	Q9S7Z2.
Family and domain databases
InterPro	IPR012463. DUF1675. [Graphical view]
Pfam	PF07897. DUF1675. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AFP4_ARATH

Accession

Primary (citable) accession number: Q9S7Z2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	May 1, 2000
Last modified:	December 14, 2011
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program