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Protein

Mitogen-activated protein kinase kinase 2

Gene

MKK2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

MEKK1, MKK1/MKK2 and MPK4 function in a signaling pathway that modulates the expression of genes responding to biotic and abiotic stresses and also plays an important role in pathogen defense by negatively regulating innate immunity. Plays a role in abiotic stress tolerance and plant disease resistance through activation of MPK4 and MPK6 by phosphorylation. Acts redundantly with MKK1.4 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated in response to cold and salt stresses through serine and threonine phosphorylation by MEKK1.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei99 – 991ATPPROSITE-ProRule annotation
Active sitei192 – 1921Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi76 – 849ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • auxin transport Source: TAIR
  • cold acclimation Source: UniProtKB
  • defense response, incompatible interaction Source: TAIR
  • innate immune response Source: UniProtKB-KW
  • response to cold Source: TAIR
  • response to salt stress Source: TAIR
  • xylem and phloem pattern formation Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Immunity, Innate immunity, Plant defense, Stress response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT4G29810-MONOMER.
ARA:GQT-2175-MONOMER.
ARA:GQT-2176-MONOMER.
ReactomeiR-ATH-110056. MAPK3 (ERK1) activation.
R-ATH-112411. MAPK1 (ERK2) activation.
R-ATH-2559580. Oxidative Stress Induced Senescence.
R-ATH-445144. Signal transduction by L1.
R-ATH-450302. activated TAK1 mediates p38 MAPK activation.
R-ATH-450321. JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1.
R-ATH-5674135. MAP2K and MAPK activation.
R-ATH-5674499. Negative feedback regulation of MAPK pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase kinase 2 (EC:2.7.12.2)
Short name:
AtMAP2Kbeta
Short name:
AtMKK2
Short name:
MAP kinase kinase 2
Gene namesi
Name:MKK2
Synonyms:MAP2K, MK1
Ordered Locus Names:At4g29810
ORF Names:F27B13.50
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G29810.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: TAIR
  • plasma membrane Source: TAIR
Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

No obvious developmental defects under normal growth conditions. Simultaneous knockdown of MKK1 and MKK2 results in dwarf and small plants exhibiting a seedling-lethality phenotype.3 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi99 – 991K → R: Loss of interaction with MEKK1. 1 Publication
Mutagenesisi220 – 2201T → A: Loss of interaction with MEKK1. 3 Publications
Mutagenesisi220 – 2201T → E: Constitutively active; when associated with E-226. 3 Publications
Mutagenesisi226 – 2261T → A: Loss of interaction with MEKK1. 3 Publications
Mutagenesisi226 – 2261T → E: Constitutively active; when associated with E-220. 3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 363363Mitogen-activated protein kinase kinase 2PRO_0000245822Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei56 – 561PhosphoserineCombined sources
Modified residuei220 – 2201Phosphothreonine3 Publications
Modified residuei226 – 2261Phosphothreonine3 Publications
Modified residuei230 – 2301PhosphothreonineBy similarity

Post-translational modificationi

Phosphorylation at Thr-220 and Thr-226 by MAP kinase kinase kinases positively regulates kinase activity. Phosphorylated by MEKK1 in response to cold (PubMed:23857079).4 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9S7U9.
PRIDEiQ9S7U9.

PTM databases

iPTMnetiQ9S7U9.

Expressioni

Gene expression databases

ExpressionAtlasiQ9S7U9. baseline and differential.
GenevisibleiQ9S7U9. AT.

Interactioni

Subunit structurei

Interacts with MEKK1, MPK4 and MPK6. May form a ternary complex composed of MEKK1 and MKK1/MKK2 and MPK4. Interacts with MPK10 and MPK11.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MPK10Q9M1Z52EBI-994350,EBI-2358527
MPK11Q9LMM52EBI-994350,EBI-2358699
MPK13Q9LQQ92EBI-994350,EBI-2358762
MPK4Q390245EBI-994350,EBI-994375
MPK6Q390265EBI-994350,EBI-349548

Protein-protein interaction databases

BioGridi14390. 7 interactions.
IntActiQ9S7U9. 8 interactions.
STRINGi3702.AT4G29810.2.

Structurei

3D structure databases

ProteinModelPortaliQ9S7U9.
SMRiQ9S7U9. Positions 64-356.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini70 – 330261Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0581. Eukaryota.
ENOG410XQ5A. LUCA.
HOGENOMiHOG000234206.
InParanoidiQ9S7U9.
KOiK04368.
OMAiEGASEAH.
PhylomeDBiQ9S7U9.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9S7U9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKKGGFSNNL KLAIPVAGEQ SITKFLTQSG TFKDGDLRVN KDGVRIISQL
60 70 80 90 100
EPEVLSPIKP ADDQLSLSDL DMVKVIGKGS SGVVQLVQHK WTGQFFALKV
110 120 130 140 150
IQLNIDEAIR KAIAQELKIN QSSQCPNLVT SYQSFYDNGA ISLILEYMDG
160 170 180 190 200
GSLADFLKSV KAIPDSYLSA IFRQVLQGLI YLHHDRHIIH RDLKPSNLLI
210 220 230 240 250
NHRGEVKITD FGVSTVMTNT AGLANTFVGT YNYMSPERIV GNKYGNKSDI
260 270 280 290 300
WSLGLVVLEC ATGKFPYAPP NQEETWTSVF ELMEAIVDQP PPALPSGNFS
310 320 330 340 350
PELSSFISTC LQKDPNSRSS AKELMEHPFL NKYDYSGINL ASYFTDAGSP
360
LATLGNLSGT FSV
Length:363
Mass (Da):39,848
Last modified:July 11, 2006 - v2
Checksum:i3E7EFC6D3A831D93
GO
Isoform 2 (identifier: Q9S7U9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     26-26: L → LRKGFGSLCR

Note: May be due to intron retention. No experimental confirmation available.
Show »
Length:372
Mass (Da):40,854
Checksum:i7D682428D1CCFE22
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti314 – 3141D → E in CAA07281 (Ref. 2) Curated
Sequence conflicti314 – 3141D → E in AAC72754 (Ref. 3) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei26 – 261L → LRKGFGSLCR in isoform 2. CuratedVSP_019784

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB015313 mRNA. Translation: BAA28828.1.
AJ006871 mRNA. Translation: CAA07281.1.
AF067792 mRNA. Translation: AAC72754.1.
AL050352 Genomic DNA. Translation: CAB43656.1.
AL161575 Genomic DNA. Translation: CAB79739.1.
CP002687 Genomic DNA. Translation: AEE85679.1.
CP002687 Genomic DNA. Translation: AEE85680.1.
AF385688 mRNA. Translation: AAK60281.1.
AY078009 mRNA. Translation: AAL77710.1.
PIRiT08542.
T51735.
RefSeqiNP_001031751.1. NM_001036674.1. [Q9S7U9-2]
NP_194710.1. NM_119127.3. [Q9S7U9-1]
UniGeneiAt.1001.
At.24272.

Genome annotation databases

EnsemblPlantsiAT4G29810.1; AT4G29810.1; AT4G29810. [Q9S7U9-1]
GeneIDi829103.
KEGGiath:AT4G29810.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB015313 mRNA. Translation: BAA28828.1.
AJ006871 mRNA. Translation: CAA07281.1.
AF067792 mRNA. Translation: AAC72754.1.
AL050352 Genomic DNA. Translation: CAB43656.1.
AL161575 Genomic DNA. Translation: CAB79739.1.
CP002687 Genomic DNA. Translation: AEE85679.1.
CP002687 Genomic DNA. Translation: AEE85680.1.
AF385688 mRNA. Translation: AAK60281.1.
AY078009 mRNA. Translation: AAL77710.1.
PIRiT08542.
T51735.
RefSeqiNP_001031751.1. NM_001036674.1. [Q9S7U9-2]
NP_194710.1. NM_119127.3. [Q9S7U9-1]
UniGeneiAt.1001.
At.24272.

3D structure databases

ProteinModelPortaliQ9S7U9.
SMRiQ9S7U9. Positions 64-356.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi14390. 7 interactions.
IntActiQ9S7U9. 8 interactions.
STRINGi3702.AT4G29810.2.

PTM databases

iPTMnetiQ9S7U9.

Proteomic databases

PaxDbiQ9S7U9.
PRIDEiQ9S7U9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G29810.1; AT4G29810.1; AT4G29810. [Q9S7U9-1]
GeneIDi829103.
KEGGiath:AT4G29810.

Organism-specific databases

TAIRiAT4G29810.

Phylogenomic databases

eggNOGiKOG0581. Eukaryota.
ENOG410XQ5A. LUCA.
HOGENOMiHOG000234206.
InParanoidiQ9S7U9.
KOiK04368.
OMAiEGASEAH.
PhylomeDBiQ9S7U9.

Enzyme and pathway databases

BioCyciARA:AT4G29810-MONOMER.
ARA:GQT-2175-MONOMER.
ARA:GQT-2176-MONOMER.
ReactomeiR-ATH-110056. MAPK3 (ERK1) activation.
R-ATH-112411. MAPK1 (ERK2) activation.
R-ATH-2559580. Oxidative Stress Induced Senescence.
R-ATH-445144. Signal transduction by L1.
R-ATH-450302. activated TAK1 mediates p38 MAPK activation.
R-ATH-450321. JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1.
R-ATH-5674135. MAP2K and MAPK activation.
R-ATH-5674499. Negative feedback regulation of MAPK pathway.

Miscellaneous databases

PROiQ9S7U9.

Gene expression databases

ExpressionAtlasiQ9S7U9. baseline and differential.
GenevisibleiQ9S7U9. AT.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiM2K2_ARATH
AccessioniPrimary (citable) accession number: Q9S7U9
Secondary accession number(s): O80395
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2006
Last sequence update: July 11, 2006
Last modified: September 7, 2016
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.