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Protein

Heat stress transcription factor A-8

Gene

HSFA8

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Transcriptional activator that specifically binds DNA sequence 5'-AGAAnnTTCT-3' known as heat shock promoter elements (HSE).

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi17 – 11296By similarityAdd
BLAST

GO - Molecular functioni

  • sequence-specific DNA binding Source: InterPro
  • transcription factor activity, sequence-specific DNA binding Source: TAIR

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Stress response, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-ATH-3371453. Regulation of HSF1-mediated heat shock response.
R-ATH-3371511. HSF1 activation.
R-ATH-3371568. Attenuation phase.
R-ATH-3371571. HSF1-dependent transactivation.

Names & Taxonomyi

Protein namesi
Recommended name:
Heat stress transcription factor A-8
Short name:
AtHsfA8
Alternative name(s):
AtHsf-03
Heat shock factor protein 5
Short name:
HSF 5
Heat shock transcription factor 5
Short name:
HSTF 5
Gene namesi
Name:HSFA8
Synonyms:HSF03, HSF5
Ordered Locus Names:At1g67970
ORF Names:T23K23.18
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G67970.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 374374Heat stress transcription factor A-8PRO_0000124586Add
BLAST

Post-translational modificationi

Exhibits temperature-dependent phosphorylation.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9S7U5.
PRIDEiQ9S7U5.

Expressioni

Gene expression databases

GenevisibleiQ9S7U5. AT.

Interactioni

Subunit structurei

Homotrimer.By similarity

Protein-protein interaction databases

BioGridi28346. 2 interactions.
MINTiMINT-8063785.
STRINGi3702.AT1G67970.1.

Structurei

3D structure databases

ProteinModelPortaliQ9S7U5.
SMRiQ9S7U5. Positions 20-93.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni126 – 19267Hydrophobic repeat HR-A/BAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi285 – 29410AHA1
Motifi298 – 3036Nuclear localization signalSequence analysis
Motifi330 – 33910AHA2
Motifi363 – 3708Nuclear export signalSequence analysis

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi7 – 104Poly-Gly
Compositional biasi11 – 166Poly-Ser
Compositional biasi291 – 2944Poly-Leu
Compositional biasi318 – 3225Poly-Glu

Domaini

The hydrophobic-rich region (HR-A/B) corresponds to the oligomerization domain. AHA motifs are transcriptional activator elements.1 Publication

Sequence similaritiesi

Belongs to the HSF family. Class A subfamily.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0627. Eukaryota.
COG5169. LUCA.
HOGENOMiHOG000029171.
InParanoidiQ9S7U5.
KOiK09419.
OMAiQPKENSW.
PhylomeDBiQ9S7U5.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR000232. HSF_DNA-bd.
IPR027725. HSF_fam.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR10015. PTHR10015. 1 hit.
PfamiPF00447. HSF_DNA-bind. 1 hit.
[Graphical view]
PRINTSiPR00056. HSFDOMAIN.
SMARTiSM00415. HSF. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS00434. HSF_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9S7U5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKSTDGGGG SSSSSSVAPF LRKCYDMVDD STTDSIISWS PSADNSFVIL
60 70 80 90 100
DTTVFSVQLL PKYFKHSNFS SFIRQLNIYG FRKVDADRWE FANDGFVRGQ
110 120 130 140 150
KDLLKNVIRR KNVQSSEQSK HESTSTTYAQ EKSGLWKEVD ILKGDKQVLA
160 170 180 190 200
QELIKVRQYQ EVTDTKMLHL EDRVQGMEES QQEMLSFLVM VMKNPSLLVQ
210 220 230 240 250
LLQPKEKNTW RKAGEGAKIV EEVTDEGESN SYGLPLVTYQ PPSDNNGTAK
260 270 280 290 300
SNSNDVNDFL RNADMLKFCL DENHVPLIIP DLYDDGAWEK LLLLSPSRKK
310 320 330 340 350
TKKQENIVKK GKDDLTLEEE EEDGTMELDK SYMLKLISEE MEKPDDFEFG
360 370
QLTPERSRNL EILTEQMELL ASNE
Length:374
Mass (Da):42,630
Last modified:May 1, 2000 - v1
Checksum:i401A924CBFEEB512
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ251866 mRNA. Translation: CAB63801.1.
AC012563 Genomic DNA. Translation: AAG51992.1.
CP002684 Genomic DNA. Translation: AEE34730.1.
AY094396 mRNA. Translation: AAM19775.1.
PIRiF96702.
RefSeqiNP_176964.1. NM_105467.3.
UniGeneiAt.5972.

Genome annotation databases

EnsemblPlantsiAT1G67970.1; AT1G67970.1; AT1G67970.
GeneIDi843125.
GrameneiAT1G67970.1; AT1G67970.1; AT1G67970.
KEGGiath:AT1G67970.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ251866 mRNA. Translation: CAB63801.1.
AC012563 Genomic DNA. Translation: AAG51992.1.
CP002684 Genomic DNA. Translation: AEE34730.1.
AY094396 mRNA. Translation: AAM19775.1.
PIRiF96702.
RefSeqiNP_176964.1. NM_105467.3.
UniGeneiAt.5972.

3D structure databases

ProteinModelPortaliQ9S7U5.
SMRiQ9S7U5. Positions 20-93.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi28346. 2 interactions.
MINTiMINT-8063785.
STRINGi3702.AT1G67970.1.

Proteomic databases

PaxDbiQ9S7U5.
PRIDEiQ9S7U5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G67970.1; AT1G67970.1; AT1G67970.
GeneIDi843125.
GrameneiAT1G67970.1; AT1G67970.1; AT1G67970.
KEGGiath:AT1G67970.

Organism-specific databases

TAIRiAT1G67970.

Phylogenomic databases

eggNOGiKOG0627. Eukaryota.
COG5169. LUCA.
HOGENOMiHOG000029171.
InParanoidiQ9S7U5.
KOiK09419.
OMAiQPKENSW.
PhylomeDBiQ9S7U5.

Enzyme and pathway databases

ReactomeiR-ATH-3371453. Regulation of HSF1-mediated heat shock response.
R-ATH-3371511. HSF1 activation.
R-ATH-3371568. Attenuation phase.
R-ATH-3371571. HSF1-dependent transactivation.

Miscellaneous databases

PROiQ9S7U5.

Gene expression databases

GenevisibleiQ9S7U5. AT.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR000232. HSF_DNA-bd.
IPR027725. HSF_fam.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR10015. PTHR10015. 1 hit.
PfamiPF00447. HSF_DNA-bind. 1 hit.
[Graphical view]
PRINTSiPR00056. HSFDOMAIN.
SMARTiSM00415. HSF. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS00434. HSF_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "De-repression of heat shock protein synthesis in transgenic plants."
    Schoeffl F., Praendl R.
    (In) Smallwood M.F., Calvert C.M., Bowles D.J. (eds.); Plant responses to environmental stress, pp.65-73, BIOS Scientific Publishers, Oxford (1999)
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
    Tissue: Green siliques.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Arabidopsis and the heat stress transcription factor world: how many heat stress transcription factors do we need?"
    Nover L., Bharti K., Doering P., Mishra S.K., Ganguli A., Scharf K.-D.
    Cell Stress Chaperones 6:177-189(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE, DOMAIN AHA.
  6. "Genome-wide analysis of heat shock transcription factor families in rice and Arabidopsis."
    Guo J., Wu J., Ji Q., Wang C., Luo L., Yuan Y., Wang Y., Wang J.
    J. Genet. Genomics 35:105-118(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiHSFA8_ARATH
AccessioniPrimary (citable) accession number: Q9S7U5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 2000
Last modified: February 17, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.