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Protein

Squamosa promoter-binding-like protein 12

Gene

SPL12

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Trans-acting factor that binds specifically to the consensus nucleotide sequence 5'-TNCGTACAA-3'.1 Publication

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi127 – 1271Zinc 1
Metal bindingi132 – 1321Zinc 1
Metal bindingi149 – 1491Zinc 1
Metal bindingi152 – 1521Zinc 1
Metal bindingi168 – 1681Zinc 2By similarity
Metal bindingi171 – 1711Zinc 2By similarity
Metal bindingi175 – 1751Zinc 2By similarity
Metal bindingi187 – 1871Zinc 2By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri124 – 20178SBP-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • transcription factor activity, sequence-specific DNA binding Source: TAIR

GO - Biological processi

  • regulation of transcription, DNA-templated Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Squamosa promoter-binding-like protein 12
Gene namesi
Name:SPL12
Ordered Locus Names:At3g60030
ORF Names:T2O9.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G60030.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 927927Squamosa promoter-binding-like protein 12PRO_0000132733Add
BLAST

Proteomic databases

PaxDbiQ9S7P5.
PRIDEiQ9S7P5.

Expressioni

Developmental stagei

Expressed during plant development.1 Publication

Gene expression databases

ExpressionAtlasiQ9S7P5. baseline and differential.
GenevisibleiQ9S7P5. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G60030.1.

Structurei

Secondary structure

1
927
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni143 – 1475Combined sources
Helixi150 – 1534Combined sources
Beta strandi158 – 1603Combined sources
Beta strandi163 – 1653Combined sources
Beta strandi169 – 1713Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WJ0NMR-A124-181[»]
ProteinModelPortaliQ9S7P5.
SMRiQ9S7P5. Positions 124-181.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9S7P5.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni124 – 20077Sufficient and necessary for DNA bindingAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi184 – 20017Bipartite nuclear localization signalSequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi362 – 39534Ser-richAdd
BLAST

Domaini

The SBP-type zinc finger is required for the binding to DNA.

Sequence similaritiesi

Contains 1 SBP-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri124 – 20178SBP-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiENOG410IIZ6. Eukaryota.
ENOG4110DQF. LUCA.
HOGENOMiHOG000082688.
InParanoidiQ9S7P5.
OMAiNGANHTE.
PhylomeDBiQ9S7P5.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
4.10.1100.10. 1 hit.
InterProiIPR020683. Ankyrin_rpt-contain_dom.
IPR004333. Transcpt_factor_SBP-box.
[Graphical view]
PfamiPF03110. SBP. 1 hit.
[Graphical view]
SUPFAMiSSF103612. SSF103612. 1 hit.
SSF48403. SSF48403. 1 hit.
PROSITEiPS51141. ZF_SBP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9S7P5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEARIEGEVE GHSLEYGFSG KRSVEWDLND WKWNGDLFVA TQLNHGSSNS
60 70 80 90 100
SSTCSDEGNV EIMERRRIEM EKKKKRRAVT VVAMEEDNLK DDDAHRLTLN
110 120 130 140 150
LGGNNIEGNG VKKTKLGGGI PSRAICCQVD NCGADLSKVK DYHRRHKVCE
160 170 180 190 200
IHSKATTALV GGIMQRFCQQ CSRFHVLEEF DEGKRSCRRR LAGHNKRRRK
210 220 230 240 250
ANPDTIGNGT SMSDDQTSNY MLITLLKILS NIHSNQSDQT GDQDLLSHLL
260 270 280 290 300
KSLVSQAGEH IGRNLVGLLQ GGGGLQASQN IGNLSALLSL EQAPREDIKH
310 320 330 340 350
HSVSETPWQE VYANSAQERV APDRSEKQVK VNDFDLNDIY IDSDDTTDIE
360 370 380 390 400
RSSPPPTNPA TSSLDYHQDS RQSSPPQTSR RNSDSASDQS PSSSSGDAQS
410 420 430 440 450
RTDRIVFKLF GKEPNDFPVA LRGQILNWLA HTPTDMESYI RPGCIVLTIY
460 470 480 490 500
LRQDEASWEE LCCDLSFSLR RLLDLSDDPL WTDGWLYLRV QNQLAFAFNG
510 520 530 540 550
QVVLDTSLPL RSHDYSQIIT VRPLAVTKKA QFTVKGINLR RPGTRLLCTV
560 570 580 590 600
EGTHLVQEAT QGGMEERDDL KENNEIDFVN FSCEMPIASG RGFMEIEDQG
610 620 630 640 650
GLSSSFFPFI VSEDEDICSE IRRLESTLEF TGTDSAMQAM DFIHEIGWLL
660 670 680 690 700
HRSELKSRLA ASDHNPEDLF SLIRFKFLIE FSMDREWCCV MKKLLNILFE
710 720 730 740 750
EGTVDPSPDA ALSELCLLHR AVRKNSKPMV EMLLRFSPKK KNQTLAGLFR
760 770 780 790 800
PDAAGPGGLT PLHIAAGKDG SEDVLDALTE DPGMTGIQAW KNSRDNTGFT
810 820 830 840 850
PEDYARLRGH FSYIHLVQRK LSRKPIAKEH VVVNIPESFN IEHKQEKRSP
860 870 880 890 900
MDSSSLEITQ INQCKLCDHK RVFVTTHHKS VAYRPAMLSM VAIAAVCVCV
910 920
ALLFKSCPEV LYVFQPFRWE LLEYGTS
Length:927
Mass (Da):104,142
Last modified:May 1, 2000 - v1
Checksum:i69354E78FA56B940
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ132096 mRNA. Translation: CAB56768.1.
AJ132097 Genomic DNA. Translation: CAB56769.1.
AL138658 Genomic DNA. Translation: CAB75918.1.
CP002686 Genomic DNA. Translation: AEE80004.1.
BT003817 mRNA. Translation: AAO41870.1.
PIRiT47827.
RefSeqiNP_191562.1. NM_115866.3.
UniGeneiAt.502.

Genome annotation databases

EnsemblPlantsiAT3G60030.1; AT3G60030.1; AT3G60030.
GeneIDi825173.
GrameneiAT3G60030.1; AT3G60030.1; AT3G60030.
KEGGiath:AT3G60030.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ132096 mRNA. Translation: CAB56768.1.
AJ132097 Genomic DNA. Translation: CAB56769.1.
AL138658 Genomic DNA. Translation: CAB75918.1.
CP002686 Genomic DNA. Translation: AEE80004.1.
BT003817 mRNA. Translation: AAO41870.1.
PIRiT47827.
RefSeqiNP_191562.1. NM_115866.3.
UniGeneiAt.502.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WJ0NMR-A124-181[»]
ProteinModelPortaliQ9S7P5.
SMRiQ9S7P5. Positions 124-181.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT3G60030.1.

Proteomic databases

PaxDbiQ9S7P5.
PRIDEiQ9S7P5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G60030.1; AT3G60030.1; AT3G60030.
GeneIDi825173.
GrameneiAT3G60030.1; AT3G60030.1; AT3G60030.
KEGGiath:AT3G60030.

Organism-specific databases

TAIRiAT3G60030.

Phylogenomic databases

eggNOGiENOG410IIZ6. Eukaryota.
ENOG4110DQF. LUCA.
HOGENOMiHOG000082688.
InParanoidiQ9S7P5.
OMAiNGANHTE.
PhylomeDBiQ9S7P5.

Miscellaneous databases

EvolutionaryTraceiQ9S7P5.
PROiQ9S7P5.

Gene expression databases

ExpressionAtlasiQ9S7P5. baseline and differential.
GenevisibleiQ9S7P5. AT.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
4.10.1100.10. 1 hit.
InterProiIPR020683. Ankyrin_rpt-contain_dom.
IPR004333. Transcpt_factor_SBP-box.
[Graphical view]
PfamiPF03110. SBP. 1 hit.
[Graphical view]
SUPFAMiSSF103612. SSF103612. 1 hit.
SSF48403. SSF48403. 1 hit.
PROSITEiPS51141. ZF_SBP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular characterization of the Arabidopsis SBP-box genes."
    Cardon G.H., Hoehmann S., Klein J., Nettesheim K., Saedler H., Huijser P.
    Gene 237:91-104(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], DEVELOPMENTAL STAGE.
    Strain: cv. Columbia.
    Tissue: Flower.
  2. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
    Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
    , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "An Arabidopsis SBP-domain fragment with a disrupted C-terminal zinc-binding site retains its tertiary structure."
    Yamasaki K., Kigawa T., Inoue M., Yamasaki T., Yabuki T., Aoki M., Seki E., Matsuda T., Tomo Y., Terada T., Shirouzu M., Tanaka A., Seki M., Shinozaki K., Yokoyama S.
    FEBS Lett. 580:2109-2116(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, REGION, STRUCTURE BY NMR OF 124-181, ZINC-BINDING SITES CYS-127; CYS-132; CYS-149 AND HIS-152.

Entry informationi

Entry nameiSPL12_ARATH
AccessioniPrimary (citable) accession number: Q9S7P5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: May 1, 2000
Last modified: May 11, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.