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Protein

Chlorophyll a-b binding protein 3, chloroplastic

Gene

LHCB3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The light-harvesting complex (LHC) functions as a light receptor, it captures and delivers excitation energy to photosystems with which it is closely associated (PubMed:26562806). Modulates the rate of photosystem II (PSII) state transitions and influences PSII macrostructure (PubMed:19880802). Involved in PSII excitation energy transfer and charge separation during photosynthesis in thylakoids (PubMed:26562806).2 Publications

Cofactori

Note: Binds at least 14 chlorophylls (8 Chl-a and 6 Chl-b) and carotenoids such as lutein and neoxanthin.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi55 – 551Magnesium (chlorophyll-b 1 axial ligand); via carbonyl oxygenBy similarity
Binding sitei77 – 771Chlorophyll-a 1; via amide nitrogenBy similarity
Binding sitei83 – 831Chlorophyll-a 1By similarity
Metal bindingi96 – 961Magnesium (chlorophyll-a 1 axial ligand)By similarity
Metal bindingi99 – 991Magnesium (chlorophyll-a 2 axial ligand)By similarity
Binding sitei101 – 1011Chlorophyll-b 2By similarity
Binding sitei135 – 1351Chlorophyll-a 3By similarity
Binding sitei145 – 1451Chlorophyll-a 3; via amide nitrogenBy similarity
Metal bindingi151 – 1511Magnesium (chlorophyll-b 2 axial ligand); via carbonyl oxygenBy similarity
Binding sitei155 – 1551Chlorophyll-b 3By similarity
Binding sitei163 – 1631Chlorophyll-b 4 or chlorophyll-b 5By similarity
Metal bindingi171 – 1711Magnesium (chlorophyll-b 3 axial ligand)By similarity
Binding sitei174 – 1741Chlorophyll-b 4By similarity
Binding sitei212 – 2121Chlorophyll-a 5By similarity
Metal bindingi213 – 2131Magnesium (chlorophyll-a 3 axial ligand)By similarity
Metal bindingi216 – 2161Magnesium (chlorophyll-a 4 axial ligand)By similarity
Binding sitei218 – 2181Chlorophyll-a 1By similarity
Metal bindingi230 – 2301Magnesium (chlorophyll-a 5 axial ligand)By similarity
Metal bindingi245 – 2451Magnesium (chlorophyll-a 6 axial ligand)By similarity
Binding sitei254 – 2541Chlorophyll-a 6; via amide nitrogenBy similarity
Binding sitei261 – 2611Chlorophyll-b 5; via carbonyl oxygenBy similarity

GO - Molecular functioni

  • chlorophyll binding Source: GO_Central
  • metal ion binding Source: UniProtKB-KW
  • pigment binding Source: GO_Central
  • structural molecule activity Source: TAIR

GO - Biological processi

  • photosynthesis Source: TAIR
  • photosynthesis, light harvesting in photosystem I Source: GO_Central
  • photosynthesis, light harvesting in photosystem II Source: UniProtKB
  • protein-chromophore linkage Source: UniProtKB-KW
  • regulation of stomatal movement Source: UniProtKB
  • response to abscisic acid Source: UniProtKB
  • response to herbicide Source: UniProtKB
  • response to high light intensity Source: UniProtKB
  • response to light stimulus Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

Photosynthesis

Keywords - Ligandi

Chlorophyll, Chromophore, Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Chlorophyll a-b binding protein 3, chloroplastic
Alternative name(s):
Light-harvesting chlorophyll B-binding protein 3Imported
Short name:
LHCB3*11 Publication
Short name:
Lhcb31 Publication
Gene namesi
Name:LHCB31 Publication
Ordered Locus Names:At5g54270Imported
ORF Names:MDK4.9Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)Imported
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G54270.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei150 – 17021HelicalSequence analysisAdd
BLAST
Transmembranei219 – 23921HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • chloroplast Source: TAIR
  • chloroplast envelope Source: GO_Central
  • chloroplast thylakoid Source: TAIR
  • chloroplast thylakoid membrane Source: TAIR
  • integral component of membrane Source: UniProtKB-KW
  • photosystem I Source: UniProtKB-KW
  • photosystem II Source: UniProtKB-KW
  • plastoglobule Source: GO_Central
  • thylakoid Source: TAIR
  • thylakoid membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Membrane, Photosystem I, Photosystem II, Plastid, Thylakoid

Pathology & Biotechi

Disruption phenotypei

In koLhcb3, accumulation of LHCB1 and LHCB2 apoproteins to adjust photosystem II (PSII) (PubMed:19880802, PubMed:26562806). However, altered macrostructural arrangement of the LHCII antenna and increased rate of PSII transition from state 1 to state 2. Increased phosphorylation level of LHCII (PubMed:19880802). Reduced responsiveness of stomatal movement to abscisic acid (ABA), therefore resulting in a decrease in tolerance to drought stress (PubMed:22143917). Deceleration of the fast phase of excitation dynamics in grana cores (PubMed:26562806).3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2222ChloroplastSequence analysisAdd
BLAST
Chaini23 – 265243Chlorophyll a-b binding protein 3, chloroplasticPRO_0000436333Add
BLAST

PTM databases

iPTMnetiQ9S7M0.

Expressioni

Inductioni

Repressed by high-light (HL) in a CAO-dependent manner (at protein level) (PubMed:16170635, PubMed:23598180). Reduced levels relative to CP43 during high light acclimation, thus helping photosystem II (PSII) structural re-arrangement (PubMed:23274453). Repressed by the herbicide bromacil (BRO) (PubMed:26802342).4 Publications

Interactioni

Subunit structurei

Present in M heterotrimers (PubMed:23274453). Forms heterotrimers with LHCB1, LHCB2, and LHCB5 proteins (PubMed:16551629, PubMed:15208324). The LHC complex consists of chlorophyll a-b binding proteins (PubMed:11245797).4 Publications

Protein-protein interaction databases

STRINGi3702.AT5G54270.1.

Structurei

3D structure databases

SMRiQ9S7M0. Positions 47-254.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IKZW. Eukaryota.
ENOG410YAI6. LUCA.
HOGENOMiHOG000238032.
KOiK08914.
OMAiRITMSNE.
OrthoDBiEOG09360J56.
PhylomeDBiQ9S7M0.

Family and domain databases

Gene3Di1.10.3460.10. 1 hit.
InterProiIPR001344. Chloro_AB-bd_pln.
IPR022796. Chloroa_b-bind.
IPR023329. Chlorophyll_a/b-bd_dom.
[Graphical view]
PANTHERiPTHR21649. PTHR21649. 1 hit.
PfamiPF00504. Chloroa_b-bind. 1 hit.
[Graphical view]
SUPFAMiSSF103511. SSF103511. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9S7M0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASTFTSSSS VLTPTTFLGQ TKASSFNPLR DVVSLGSPKY TMGNDLWYGP
60 70 80 90 100
DRVKYLGPFS VQTPSYLTGE FPGDYGWDTA GLSADPEAFA KNRALEVIHG
110 120 130 140 150
RWAMLGAFGC ITPEVLQKWV RVDFKEPVWF KAGSQIFSEG GLDYLGNPNL
160 170 180 190 200
VHAQSILAVL GFQVILMGLV EGFRINGLDG VGEGNDLYPG GQYFDPLGLA
210 220 230 240 250
DDPVTFAELK VKEIKNGRLA MFSMFGFFVQ AIVTGKGPLE NLLDHLDNPV
260
ANNAWAFATK FAPGA
Length:265
Mass (Da):28,707
Last modified:May 1, 2000 - v1
Checksum:i75593036C6AFBE25
GO
Isoform 2 (identifier: Q9S7M0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-103: Missing.

Note: No experimental confirmation available.
Show »
Length:162
Mass (Da):17,537
Checksum:i28B5C9789112BA35
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 103103Missing in isoform 2. VSP_058349Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF143691 mRNA. Translation: AAD37362.1.
AF134126 mRNA. Translation: AAD28773.1.
AB010695 Genomic DNA. Translation: BAB10750.1.
CP002688 Genomic DNA. Translation: AED96477.1.
AF361858 mRNA. Translation: AAK32870.1.
AF372917 mRNA. Translation: AAK49633.1.
AY057735 mRNA. Translation: AAL15365.1.
AK318653 mRNA. Translation: BAH56768.1.
Z18479 mRNA. Translation: CAA79201.1.
PIRiT52318.
RefSeqiNP_200238.1. NM_124807.3. [Q9S7M0-1]
UniGeneiAt.10812.
At.21173.
At.32199.
At.49175.
At.66422.
At.70236.

Genome annotation databases

EnsemblPlantsiAT5G54270.1; AT5G54270.1; AT5G54270. [Q9S7M0-1]
GeneIDi835515.
KEGGiath:AT5G54270.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF143691 mRNA. Translation: AAD37362.1.
AF134126 mRNA. Translation: AAD28773.1.
AB010695 Genomic DNA. Translation: BAB10750.1.
CP002688 Genomic DNA. Translation: AED96477.1.
AF361858 mRNA. Translation: AAK32870.1.
AF372917 mRNA. Translation: AAK49633.1.
AY057735 mRNA. Translation: AAL15365.1.
AK318653 mRNA. Translation: BAH56768.1.
Z18479 mRNA. Translation: CAA79201.1.
PIRiT52318.
RefSeqiNP_200238.1. NM_124807.3. [Q9S7M0-1]
UniGeneiAt.10812.
At.21173.
At.32199.
At.49175.
At.66422.
At.70236.

3D structure databases

SMRiQ9S7M0. Positions 47-254.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G54270.1.

PTM databases

iPTMnetiQ9S7M0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G54270.1; AT5G54270.1; AT5G54270. [Q9S7M0-1]
GeneIDi835515.
KEGGiath:AT5G54270.

Organism-specific databases

TAIRiAT5G54270.

Phylogenomic databases

eggNOGiENOG410IKZW. Eukaryota.
ENOG410YAI6. LUCA.
HOGENOMiHOG000238032.
KOiK08914.
OMAiRITMSNE.
OrthoDBiEOG09360J56.
PhylomeDBiQ9S7M0.

Family and domain databases

Gene3Di1.10.3460.10. 1 hit.
InterProiIPR001344. Chloro_AB-bd_pln.
IPR022796. Chloroa_b-bind.
IPR023329. Chlorophyll_a/b-bd_dom.
[Graphical view]
PANTHERiPTHR21649. PTHR21649. 1 hit.
PfamiPF00504. Chloroa_b-bind. 1 hit.
[Graphical view]
SUPFAMiSSF103511. SSF103511. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCB3_ARATH
AccessioniPrimary (citable) accession number: Q9S7M0
Secondary accession number(s): C0Z239, Q42016
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 8, 2016
Last sequence update: May 1, 2000
Last modified: September 7, 2016
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.