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Protein

Chlorophyll a-b binding protein 3, chloroplastic

Gene

LHCB3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The light-harvesting complex (LHC) functions as a light receptor, it captures and delivers excitation energy to photosystems with which it is closely associated (PubMed:26562806). Modulates the rate of photosystem II (PSII) state transitions and influences PSII macrostructure (PubMed:19880802). Involved in PSII excitation energy transfer and charge separation during photosynthesis in thylakoids (PubMed:26562806).2 Publications

Cofactori

Note: Binds at least 14 chlorophylls (8 Chl-a and 6 Chl-b) and carotenoids such as lutein and neoxanthin.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi55Magnesium (chlorophyll-b 1 axial ligand); via carbonyl oxygenBy similarity1
Binding sitei77Chlorophyll-a 1; via amide nitrogenBy similarity1
Binding sitei83Chlorophyll-a 1By similarity1
Metal bindingi96Magnesium (chlorophyll-a 1 axial ligand)By similarity1
Metal bindingi99Magnesium (chlorophyll-a 2 axial ligand)By similarity1
Binding sitei101Chlorophyll-b 2By similarity1
Binding sitei135Chlorophyll-a 3By similarity1
Binding sitei145Chlorophyll-a 3; via amide nitrogenBy similarity1
Metal bindingi151Magnesium (chlorophyll-b 2 axial ligand); via carbonyl oxygenBy similarity1
Binding sitei155Chlorophyll-b 3By similarity1
Binding sitei163Chlorophyll-b 4 or chlorophyll-b 5By similarity1
Metal bindingi171Magnesium (chlorophyll-b 3 axial ligand)By similarity1
Binding sitei174Chlorophyll-b 4By similarity1
Binding sitei212Chlorophyll-a 5By similarity1
Metal bindingi213Magnesium (chlorophyll-a 3 axial ligand)By similarity1
Metal bindingi216Magnesium (chlorophyll-a 4 axial ligand)By similarity1
Binding sitei218Chlorophyll-a 1By similarity1
Metal bindingi230Magnesium (chlorophyll-a 5 axial ligand)By similarity1
Metal bindingi245Magnesium (chlorophyll-a 6 axial ligand)By similarity1
Binding sitei254Chlorophyll-a 6; via amide nitrogenBy similarity1
Binding sitei261Chlorophyll-b 5; via carbonyl oxygenBy similarity1

GO - Molecular functioni

  • chlorophyll binding Source: GO_Central
  • metal ion binding Source: UniProtKB-KW
  • pigment binding Source: GO_Central
  • protein domain specific binding Source: CAFA
  • structural molecule activity Source: TAIR

GO - Biological processi

  • photosynthesis Source: TAIR
  • photosynthesis, light harvesting in photosystem I Source: GO_Central
  • photosynthesis, light harvesting in photosystem II Source: UniProtKB
  • protein-chromophore linkage Source: UniProtKB-KW
  • regulation of stomatal movement Source: UniProtKB
  • response to abscisic acid Source: UniProtKB
  • response to herbicide Source: UniProtKB
  • response to high light intensity Source: UniProtKB

Keywordsi

Biological processPhotosynthesis
LigandChlorophyll, Chromophore, Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Chlorophyll a-b binding protein 3, chloroplastic
Alternative name(s):
Light-harvesting chlorophyll B-binding protein 3Imported
Short name:
LHCB3*11 Publication
Short name:
Lhcb31 Publication
Gene namesi
Name:LHCB31 Publication
Ordered Locus Names:At5g54270Imported
ORF Names:MDK4.9Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)Imported
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G54270.
TAIRilocus:2162540. AT5G54270.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei150 – 170HelicalSequence analysisAdd BLAST21
Transmembranei219 – 239HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Chloroplast, Membrane, Photosystem I, Photosystem II, Plastid, Thylakoid

Pathology & Biotechi

Disruption phenotypei

In koLhcb3, accumulation of LHCB1 and LHCB2 apoproteins to adjust photosystem II (PSII) (PubMed:19880802, PubMed:26562806). However, altered macrostructural arrangement of the LHCII antenna and increased rate of PSII transition from state 1 to state 2. Increased phosphorylation level of LHCII (PubMed:19880802). Reduced responsiveness of stomatal movement to abscisic acid (ABA), therefore resulting in a decrease in tolerance to drought stress (PubMed:22143917). Deceleration of the fast phase of excitation dynamics in grana cores (PubMed:26562806).3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 22ChloroplastSequence analysisAdd BLAST22
ChainiPRO_000043633323 – 265Chlorophyll a-b binding protein 3, chloroplasticAdd BLAST243

Proteomic databases

PaxDbiQ9S7M0.

PTM databases

iPTMnetiQ9S7M0.

Expressioni

Inductioni

Repressed by high-light (HL) in a CAO-dependent manner (at protein level) (PubMed:16170635, PubMed:23598180). Reduced levels relative to CP43 during high light acclimation, thus helping photosystem II (PSII) structural re-arrangement (PubMed:23274453). Repressed by the herbicide bromacil (BRO) (PubMed:26802342).4 Publications

Gene expression databases

ExpressionAtlasiQ9S7M0. baseline and differential.

Interactioni

Subunit structurei

Present in M heterotrimers (PubMed:23274453). Forms heterotrimers with LHCB1, LHCB2, and LHCB5 proteins (PubMed:16551629, PubMed:15208324). The LHC complex consists of chlorophyll a-b binding proteins (PubMed:11245797).4 Publications

GO - Molecular functioni

  • protein domain specific binding Source: CAFA

Protein-protein interaction databases

STRINGi3702.AT5G54270.1.

Structurei

3D structure databases

ProteinModelPortaliQ9S7M0.
SMRiQ9S7M0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IKZW. Eukaryota.
ENOG410YAI6. LUCA.
HOGENOMiHOG000238032.
KOiK08914.
OMAiRITMSND.
OrthoDBiEOG09360J56.
PhylomeDBiQ9S7M0.

Family and domain databases

Gene3Di1.10.3460.10. 1 hit.
InterProiView protein in InterPro
IPR001344. Chloro_AB-bd_pln.
IPR022796. Chloroa_b-bind.
IPR023329. Chlorophyll_a/b-bd_dom_sf.
PANTHERiPTHR21649. PTHR21649. 1 hit.
PfamiView protein in Pfam
PF00504. Chloroa_b-bind. 1 hit.
SUPFAMiSSF103511. SSF103511. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9S7M0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASTFTSSSS VLTPTTFLGQ TKASSFNPLR DVVSLGSPKY TMGNDLWYGP
60 70 80 90 100
DRVKYLGPFS VQTPSYLTGE FPGDYGWDTA GLSADPEAFA KNRALEVIHG
110 120 130 140 150
RWAMLGAFGC ITPEVLQKWV RVDFKEPVWF KAGSQIFSEG GLDYLGNPNL
160 170 180 190 200
VHAQSILAVL GFQVILMGLV EGFRINGLDG VGEGNDLYPG GQYFDPLGLA
210 220 230 240 250
DDPVTFAELK VKEIKNGRLA MFSMFGFFVQ AIVTGKGPLE NLLDHLDNPV
260
ANNAWAFATK FAPGA
Length:265
Mass (Da):28,707
Last modified:May 1, 2000 - v1
Checksum:i75593036C6AFBE25
GO
Isoform 2 (identifier: Q9S7M0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-103: Missing.

Note: No experimental confirmation available.
Show »
Length:162
Mass (Da):17,537
Checksum:i28B5C9789112BA35
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0583491 – 103Missing in isoform 2. Add BLAST103

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF143691 mRNA. Translation: AAD37362.1.
AF134126 mRNA. Translation: AAD28773.1.
AB010695 Genomic DNA. Translation: BAB10750.1.
CP002688 Genomic DNA. Translation: AED96477.1.
AF361858 mRNA. Translation: AAK32870.1.
AF372917 mRNA. Translation: AAK49633.1.
AY057735 mRNA. Translation: AAL15365.1.
AK318653 mRNA. Translation: BAH56768.1.
Z18479 mRNA. Translation: CAA79201.1.
PIRiT52318.
RefSeqiNP_200238.1. NM_124807.4. [Q9S7M0-1]
UniGeneiAt.10812.
At.21173.
At.32199.
At.49175.
At.66422.
At.70236.

Genome annotation databases

EnsemblPlantsiAT5G54270.1; AT5G54270.1; AT5G54270. [Q9S7M0-1]
GeneIDi835515.
GrameneiAT5G54270.1; AT5G54270.1; AT5G54270.
KEGGiath:AT5G54270.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCB3_ARATH
AccessioniPrimary (citable) accession number: Q9S7M0
Secondary accession number(s): C0Z239, Q42016
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 8, 2016
Last sequence update: May 1, 2000
Last modified: November 22, 2017
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families