Q9S7L9 (CX6B1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome c oxidase subunit 6b-1
Short name=AtCOX6b-1

Gene names

Name:	COX6B-1
Ordered Locus Names:	At1g22450
ORF Names:	F12K8.20

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	191 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. This protein may be one of the heme-binding subunits of the oxidase.
Subcellular location	Mitochondrion By similarity.
Tissue specificity	Expressed in the whole plant. Ref.1
Developmental stage	Gradually expressed after germination. Ref.6
Sequence similarities	Belongs to the cytochrome c oxidase subunit 6B (TC 3.D.4.8) family. [View classification]

Ontologies

Keywords
Cellular component	Mitochondrion
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	aerobic respiration Inferred from electronic annotation. Source: EnsemblPlants/Gramene mitochondrial electron transport, cytochrome c to oxygen Inferred from electronic annotation. Source: EnsemblPlants/Gramene response to salt stress Inferred from expression pattern PubMed 17916636. Source: TAIR
Cellular_component	chloroplast thylakoid membrane Inferred from direct assay PubMed 14729914. Source: TAIR mitochondrial respiratory chain complex IV Inferred from electronic annotation. Source: EnsemblPlants/Gramene mitochondrion Inferred from direct assay PubMed 22923678. Source: TAIR
Molecular_function	copper ion binding Inferred from direct assay PubMed 20018591. Source: TAIR cytochrome-c oxidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 191	191	Cytochrome c oxidase subunit 6b-1		PRO_0000412233
Regions
	Compositional bias	32 – 120	89	Glu-rich
Amino acid modifications
	Disulfide bond	137 ↔ 169		By similarity
	Disulfide bond	147 ↔ 158		By similarity
Experimental info
	Sequence conflict	87	1	E → Q in AAM63485. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Q9S7L9 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 7592D7DBC94BC054	FASTA	191	21,195
	10 20 30 40 50 60 MADAVNAQTP SLSEQYHLEK EVKQDTSAKP VEVKEVAPEV TTQAEEVKTE QAKEESPVEE 70 80 90 100 110 120 AVSVVEEKSE SAPESTEVAS EAPAAAEDNA EETPAAAEEN NDENASEEVA EETPDEIKLE 130 140 150 160 170 180 TAPADFRFPT TNQTRHCFTR YVEYHRCVAA KGDDAPECDK FAKFYRSLCP SEWVDRWNEQ 190 RENGTFPGPL P

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization and expression of the genes for cytochrome c oxidase subunit VIb (COX6b) from rice and Arabidopsis thaliana." Ohtsu K., Nakazono M., Tsutsumi N., Hirai A. Gene 264:233-239(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], GENE FAMILY, NOMENCLATURE, TISSUE SPECIFICITY.
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"The gene for alternative oxidase-2 (AOX2) from Arabidopsis thaliana consists of five exons unlike other AOX genes and is transcribed at an early stage during germination." Saisho D., Nakazono M., Lee K.-H., Tsutsumi N., Akita S., Hirai A. Genes Genet. Syst. 76:89-97(2001) [PubMed] [Europe PMC] [Abstract] Cited for: DEVELOPMENTAL STAGE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB035444 mRNA. Translation: BAA87883.1. AC006551 Genomic DNA. Translation: AAF18532.1. CP002684 Genomic DNA. Translation: AEE30243.1. AY063997 mRNA. Translation: AAL36353.1. AY096696 mRNA. Translation: AAM20330.1. AY086483 mRNA. Translation: AAM63485.1.
IPI	IPI00530973.
PIR	F86357.
RefSeq	NP_173661.1. NM_102094.3.
UniGene	At.10491. At.67078.
3D structure databases
ProteinModelPortal	Q9S7L9.
SMR	Q9S7L9. Positions 120-190.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9S7L9. 1 interaction.
STRING	3702.AT1G22450.1-P.
Proteomic databases
PRIDE	Q9S7L9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G22450.1; AT1G22450.1; AT1G22450.
GeneID	838851.
KEGG	ath:AT1G22450.
Organism-specific databases
GeneFarm	3578.
TAIR	At1g22450.
Phylogenomic databases
eggNOG	NOG279294.
HOGENOM	HOG000070575.
InParanoid	Q9S7L9.
KO	K02267.
OMA	DDAPECD.
ProtClustDB	CLSN2681837.
Gene expression databases
ArrayExpress	Q9S7L9.
Genevestigator	Q9S7L9.
Family and domain databases
Gene3D	1.10.10.140. 1 hit.
InterPro	IPR003213. Cyt_c_oxidase_su6B. [Graphical view]
Pfam	PF02297. COX6B. 1 hit. [Graphical view]
SUPFAM	SSF47694. Cyt_c_oxidase_6B. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CX6B1_ARATH

Accession

Primary (citable) accession number: Q9S7L9
Secondary accession number(s): Q8LCP1

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 21, 2011
Last sequence update:	May 1, 2000
Last modified:	May 1, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program