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Q9S7J8

- HMA7_ARATH

UniProt

Q9S7J8 - HMA7_ARATH

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Protein

Copper-transporting ATPase RAN1

Gene

RAN1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in copper import into the cell. Essential for ethylene signaling, which requires copper. Acts by delivering copper to create functional hormone receptors.

Catalytic activityi

ATP + H2O + Cu+(Side 1) = ADP + phosphate + Cu+(Side 2).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi67 – 671CopperPROSITE-ProRule annotation
Metal bindingi70 – 701CopperPROSITE-ProRule annotation
Metal bindingi144 – 1441CopperPROSITE-ProRule annotation
Metal bindingi147 – 1471CopperPROSITE-ProRule annotation
Active sitei658 – 65814-aspartylphosphate intermediateBy similarity
Metal bindingi877 – 8771MagnesiumPROSITE-ProRule annotation
Metal bindingi881 – 8811MagnesiumPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cation-transporting ATPase activity Source: InterPro
  3. copper ion binding Source: InterPro
  4. copper ion transmembrane transporter activity Source: TAIR

GO - Biological processi

  1. copper ion transmembrane transport Source: GOC
  2. ethylene-activated signaling pathway Source: TAIR
  3. regulation of stomatal movement Source: TAIR
  4. response to ethylene Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Copper transport, Ethylene signaling pathway, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Copper, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT5G44790-MONOMER.

Protein family/group databases

TCDBi3.A.3.5.32. the p-type atpase (p-atpase) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Copper-transporting ATPase RAN1 (EC:3.6.3.54)
Alternative name(s):
Protein HEAVY METAL ATPASE 7
Protein RESPONSIVE TO ANTAGONIST 1
Gene namesi
Name:RAN1
Synonyms:HMA7
Ordered Locus Names:At5g44790
ORF Names:K23L20.14, T19K24.18
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G44790.

Subcellular locationi

GO - Cellular componenti

  1. endosome Source: TAIR
  2. Golgi apparatus Source: TAIR
  3. integral component of membrane Source: UniProtKB-KW
  4. trans-Golgi network Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi173 – 1731G → E in ran1-2. 1 Publication
Mutagenesisi497 – 4971T → I in ran1-1. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10011001Copper-transporting ATPase RAN1PRO_0000046402Add
BLAST

Proteomic databases

PaxDbiQ9S7J8.
PRIDEiQ9S7J8.

Expressioni

Gene expression databases

GenevestigatoriQ9S7J8.

Interactioni

Protein-protein interaction databases

BioGridi19759. 1 interaction.
IntActiQ9S7J8. 2 interactions.
MINTiMINT-8066708.

Structurei

Secondary structure

1
1001
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi56 – 638Combined sources
Helixi68 – 7912Combined sources
Beta strandi84 – 907Combined sources
Helixi91 – 933Combined sources
Beta strandi95 – 1006Combined sources
Turni102 – 1043Combined sources
Helixi107 – 11711Combined sources
Beta strandi120 – 1267Combined sources

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3DXSX-ray1.70X56-128[»]
ProteinModelPortaliQ9S7J8.
SMRiQ9S7J8. Positions 55-270, 291-986.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9S7J8.

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 298298CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini321 – 33818ExtracellularSequence AnalysisAdd
BLAST
Topological domaini359 – 3657CytoplasmicSequence Analysis
Topological domaini387 – 40317ExtracellularSequence AnalysisAdd
BLAST
Topological domaini425 – 558134CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini582 – 60221ExtracellularSequence AnalysisAdd
BLAST
Topological domaini621 – 931311CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini952 – 96312ExtracellularSequence AnalysisAdd
BLAST
Topological domaini983 – 100119CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei299 – 32022HelicalSequence AnalysisAdd
BLAST
Transmembranei339 – 35820HelicalSequence AnalysisAdd
BLAST
Transmembranei366 – 38621HelicalSequence AnalysisAdd
BLAST
Transmembranei404 – 42421HelicalSequence AnalysisAdd
BLAST
Transmembranei559 – 58123HelicalSequence AnalysisAdd
BLAST
Transmembranei603 – 62018HelicalSequence AnalysisAdd
BLAST
Transmembranei932 – 95120HelicalSequence AnalysisAdd
BLAST
Transmembranei964 – 98219HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini57 – 12367HMA 1PROSITE-ProRule annotationAdd
BLAST
Domaini134 – 20067HMA 2PROSITE-ProRule annotationAdd
BLAST
Domaini208 – 27467HMA 3; degeneratePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 3 HMA domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG2217.
HOGENOMiHOG000250397.
InParanoidiQ9S7J8.
KOiK17686.
OMAiMSIMYLA.
PhylomeDBiQ9S7J8.

Family and domain databases

Gene3Di2.70.150.10. 1 hit.
3.40.1110.10. 1 hit.
3.40.50.1000. 2 hits.
InterProiIPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR027256. Cation_transp_P-typ_ATPase_IB.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
IPR017969. Heavy-metal-associated_CS.
IPR006121. HeavyMe-assoc_HMA.
IPR006122. HMA_Cu_ion-bd.
[Graphical view]
PfamiPF00122. E1-E2_ATPase. 1 hit.
PF00403. HMA. 2 hits.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSiPR00119. CATATPASE.
SUPFAMiSSF55008. SSF55008. 3 hits.
SSF56784. SSF56784. 2 hits.
TIGRFAMsiTIGR01525. ATPase-IB_hvy. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
TIGR00003. TIGR00003. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
PS01047. HMA_1. 2 hits.
PS50846. HMA_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9S7J8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAPSRRDLQL TPVTGGSSSQ ISDMEEVGLL DSYHNEANAD DILTKIEEGR
60 70 80 90 100
DVSGLRKIQV GVTGMTCAAC SNSVEAALMN VNGVFKASVA LLQNRADVVF
110 120 130 140 150
DPNLVKEEDI KEAIEDAGFE AEILAEEQTQ ATLVGQFTIG GMTCAACVNS
160 170 180 190 200
VEGILRDLPG VKRAVVALST SLGEVEYDPN VINKDDIVNA IEDAGFEGSL
210 220 230 240 250
VQSNQQDKLV LRVDGILNEL DAQVLEGILT RLNGVRQFRL DRISGELEVV
260 270 280 290 300
FDPEVVSSRS LVDGIEEDGF GKFKLRVMSP YERLSSKDTG EASNMFRRFI
310 320 330 340 350
SSLVLSIPLF FIQVICPHIA LFDALLVWRC GPFMMGDWLK WALVSVIQFV
360 370 380 390 400
IGKRFYVAAW RALRNGSTNM DVLVALGTSA SYFYSVGALL YGAVTGFWSP
410 420 430 440 450
TYFDASAMLI TFVLLGKYLE SLAKGKTSDA MKKLVQLTPA TAILLTEGKG
460 470 480 490 500
GKLVGEREID ALLIQPGDTL KVHPGAKIPA DGVVVWGSSY VNESMVTGES
510 520 530 540 550
VPVSKEVDSP VIGGTINMHG ALHMKATKVG SDAVLSQIIS LVETAQMSKA
560 570 580 590 600
PIQKFADYVA SIFVPVVITL ALFTLVGWSI GGAVGAYPDE WLPENGTHFV
610 620 630 640 650
FSLMFSISVV VIACPCALGL ATPTAVMVAT GVGATNGVLI KGGDALEKAH
660 670 680 690 700
KVKYVIFDKT GTLTQGKATV TTTKVFSEMD RGEFLTLVAS AEASSEHPLA
710 720 730 740 750
KAIVAYARHF HFFDESTEDG ETNNKDLQNS GWLLDTSDFS ALPGKGIQCL
760 770 780 790 800
VNEKMILVGN RKLMSENAIN IPDHVEKFVE DLEESGKTGV IVAYNGKLVG
810 820 830 840 850
VMGIADPLKR EAALVVEGLL RMGVRPIMVT GDNWRTARAV AKEVGIEDVR
860 870 880 890 900
AEVMPAGKAD VIRSLQKDGS TVAMVGDGIN DSPALAAADV GMAIGAGTDV
910 920 930 940 950
AIEAADYVLM RNNLEDVITA IDLSRKTLTR IRLNYVFAMA YNVVSIPIAA
960 970 980 990 1000
GVFFPVLRVQ LPPWAAGACM ALSSVSVVCS SLLLRRYKKP RLTTVLKITT

E
Length:1,001
Mass (Da):107,395
Last modified:May 1, 2000 - v1
Checksum:i7E820909C60D5B0F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF091112 Genomic DNA. Translation: AAD29115.1.
AF082565 mRNA. Translation: AAD29109.1.
AB016874 Genomic DNA. Translation: BAB08832.1.
AC002342 Genomic DNA. Translation: AAC79141.2.
CP002688 Genomic DNA. Translation: AED95163.1.
RefSeqiNP_199292.1. NM_123847.2.
UniGeneiAt.24482.

Genome annotation databases

EnsemblPlantsiAT5G44790.1; AT5G44790.1; AT5G44790.
GeneIDi834509.
KEGGiath:AT5G44790.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF091112 Genomic DNA. Translation: AAD29115.1 .
AF082565 mRNA. Translation: AAD29109.1 .
AB016874 Genomic DNA. Translation: BAB08832.1 .
AC002342 Genomic DNA. Translation: AAC79141.2 .
CP002688 Genomic DNA. Translation: AED95163.1 .
RefSeqi NP_199292.1. NM_123847.2.
UniGenei At.24482.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3DXS X-ray 1.70 X 56-128 [» ]
ProteinModelPortali Q9S7J8.
SMRi Q9S7J8. Positions 55-270, 291-986.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 19759. 1 interaction.
IntActi Q9S7J8. 2 interactions.
MINTi MINT-8066708.

Protein family/group databases

TCDBi 3.A.3.5.32. the p-type atpase (p-atpase) superfamily.

Proteomic databases

PaxDbi Q9S7J8.
PRIDEi Q9S7J8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G44790.1 ; AT5G44790.1 ; AT5G44790 .
GeneIDi 834509.
KEGGi ath:AT5G44790.

Organism-specific databases

TAIRi AT5G44790.

Phylogenomic databases

eggNOGi COG2217.
HOGENOMi HOG000250397.
InParanoidi Q9S7J8.
KOi K17686.
OMAi MSIMYLA.
PhylomeDBi Q9S7J8.

Enzyme and pathway databases

BioCyci ARA:AT5G44790-MONOMER.

Miscellaneous databases

EvolutionaryTracei Q9S7J8.
PROi Q9S7J8.

Gene expression databases

Genevestigatori Q9S7J8.

Family and domain databases

Gene3Di 2.70.150.10. 1 hit.
3.40.1110.10. 1 hit.
3.40.50.1000. 2 hits.
InterProi IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR027256. Cation_transp_P-typ_ATPase_IB.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
IPR017969. Heavy-metal-associated_CS.
IPR006121. HeavyMe-assoc_HMA.
IPR006122. HMA_Cu_ion-bd.
[Graphical view ]
Pfami PF00122. E1-E2_ATPase. 1 hit.
PF00403. HMA. 2 hits.
PF00702. Hydrolase. 1 hit.
[Graphical view ]
PRINTSi PR00119. CATATPASE.
SUPFAMi SSF55008. SSF55008. 3 hits.
SSF56784. SSF56784. 2 hits.
TIGRFAMsi TIGR01525. ATPase-IB_hvy. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
TIGR00003. TIGR00003. 2 hits.
PROSITEi PS00154. ATPASE_E1_E2. 1 hit.
PS01047. HMA_1. 2 hits.
PS50846. HMA_2. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Responsive-to-antagonist 1, a Menkes/Wilson disease-related copper transporter, is required for ethylene signaling in Arabidopsis."
    Hirayama T., Kieber J.J., Hirayama N., Kogan M., Guzman P., Nourizadeh S., Alonso J.M., Dailey W.P., Dancis A., Ecker J.R.
    Cell 97:383-393(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], MUTAGENESIS.
    Strain: cv. Columbia.
  2. "Structural analysis of Arabidopsis thaliana chromosome 5. VIII. Sequence features of the regions of 1,081,958 bp covered by seventeen physically assigned P1 and TAC clones."
    Asamizu E., Sato S., Kaneko T., Nakamura Y., Kotani H., Miyajima N., Tabata S.
    DNA Res. 5:379-391(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
    Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
    , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
    Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.

Entry informationi

Entry nameiHMA7_ARATH
AccessioniPrimary (citable) accession number: Q9S7J8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: May 1, 2000
Last modified: October 29, 2014
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3