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Protein

Copper-transporting ATPase RAN1

Gene

RAN1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in copper import into the cell. Essential for ethylene signaling, which requires copper. Acts by delivering copper to create functional hormone receptors.

Catalytic activityi

ATP + H2O + Cu+(Side 1) = ADP + phosphate + Cu+(Side 2).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi67CopperPROSITE-ProRule annotation1
Metal bindingi70CopperPROSITE-ProRule annotation1
Metal bindingi144CopperPROSITE-ProRule annotation1
Metal bindingi147CopperPROSITE-ProRule annotation1
Active sitei6584-aspartylphosphate intermediateBy similarity1
Metal bindingi877MagnesiumPROSITE-ProRule annotation1
Metal bindingi881MagnesiumPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • ethylene-activated signaling pathway Source: TAIR
  • regulation of stomatal movement Source: TAIR
  • response to ethylene Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Copper transport, Ethylene signaling pathway, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Copper, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT5G44790-MONOMER.
ReactomeiR-ATH-936837. Ion transport by P-type ATPases.

Protein family/group databases

TCDBi3.A.3.5.32. the p-type atpase (p-atpase) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Copper-transporting ATPase RAN1 (EC:3.6.3.54)
Alternative name(s):
Protein HEAVY METAL ATPASE 7
Protein RESPONSIVE TO ANTAGONIST 1
Gene namesi
Name:RAN1
Synonyms:HMA7
Ordered Locus Names:At5g44790
ORF Names:K23L20.14, T19K24.18
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G44790.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 298CytoplasmicSequence analysisAdd BLAST298
Transmembranei299 – 320HelicalSequence analysisAdd BLAST22
Topological domaini321 – 338ExtracellularSequence analysisAdd BLAST18
Transmembranei339 – 358HelicalSequence analysisAdd BLAST20
Topological domaini359 – 365CytoplasmicSequence analysis7
Transmembranei366 – 386HelicalSequence analysisAdd BLAST21
Topological domaini387 – 403ExtracellularSequence analysisAdd BLAST17
Transmembranei404 – 424HelicalSequence analysisAdd BLAST21
Topological domaini425 – 558CytoplasmicSequence analysisAdd BLAST134
Transmembranei559 – 581HelicalSequence analysisAdd BLAST23
Topological domaini582 – 602ExtracellularSequence analysisAdd BLAST21
Transmembranei603 – 620HelicalSequence analysisAdd BLAST18
Topological domaini621 – 931CytoplasmicSequence analysisAdd BLAST311
Transmembranei932 – 951HelicalSequence analysisAdd BLAST20
Topological domaini952 – 963ExtracellularSequence analysisAdd BLAST12
Transmembranei964 – 982HelicalSequence analysisAdd BLAST19
Topological domaini983 – 1001CytoplasmicSequence analysisAdd BLAST19

GO - Cellular componenti

  • endosome Source: TAIR
  • Golgi apparatus Source: TAIR
  • integral component of plasma membrane Source: GO_Central
  • trans-Golgi network Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi173G → E in ran1-2. 1 Publication1
Mutagenesisi497T → I in ran1-1. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000464021 – 1001Copper-transporting ATPase RAN1Add BLAST1001

Proteomic databases

PaxDbiQ9S7J8.
PRIDEiQ9S7J8.

PTM databases

SwissPalmiQ9S7J8.

Expressioni

Gene expression databases

GenevisibleiQ9S7J8. AT.

Interactioni

Protein-protein interaction databases

BioGridi19759. 3 interactors.
IntActiQ9S7J8. 2 interactors.
MINTiMINT-8066708.
STRINGi3702.AT5G44790.1.

Structurei

Secondary structure

11001
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi56 – 63Combined sources8
Helixi68 – 79Combined sources12
Beta strandi84 – 90Combined sources7
Helixi91 – 93Combined sources3
Beta strandi95 – 100Combined sources6
Turni102 – 104Combined sources3
Helixi107 – 117Combined sources11
Beta strandi120 – 126Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3DXSX-ray1.70X56-128[»]
ProteinModelPortaliQ9S7J8.
SMRiQ9S7J8.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9S7J8.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini57 – 123HMA 1PROSITE-ProRule annotationAdd BLAST67
Domaini134 – 200HMA 2PROSITE-ProRule annotationAdd BLAST67
Domaini208 – 274HMA 3; degeneratePROSITE-ProRule annotationAdd BLAST67

Sequence similaritiesi

Contains 3 HMA domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0207. Eukaryota.
COG2217. LUCA.
HOGENOMiHOG000250397.
InParanoidiQ9S7J8.
KOiK17686.
OMAiNEKMILV.
OrthoDBiEOG093602FP.
PhylomeDBiQ9S7J8.

Family and domain databases

CDDicd00371. HMA. 2 hits.
Gene3Di2.70.150.10. 1 hit.
3.40.1110.10. 1 hit.
3.40.50.1000. 2 hits.
InterProiIPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR017969. Heavy-metal-associated_CS.
IPR006122. HMA_Cu_ion-bd.
IPR006121. HMA_dom.
IPR027256. P-typ_ATPase_IB.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00122. E1-E2_ATPase. 1 hit.
PF00403. HMA. 2 hits.
[Graphical view]
SUPFAMiSSF55008. SSF55008. 3 hits.
SSF56784. SSF56784. 2 hits.
TIGRFAMsiTIGR01525. ATPase-IB_hvy. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
TIGR00003. TIGR00003. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
PS01047. HMA_1. 2 hits.
PS50846. HMA_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9S7J8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPSRRDLQL TPVTGGSSSQ ISDMEEVGLL DSYHNEANAD DILTKIEEGR
60 70 80 90 100
DVSGLRKIQV GVTGMTCAAC SNSVEAALMN VNGVFKASVA LLQNRADVVF
110 120 130 140 150
DPNLVKEEDI KEAIEDAGFE AEILAEEQTQ ATLVGQFTIG GMTCAACVNS
160 170 180 190 200
VEGILRDLPG VKRAVVALST SLGEVEYDPN VINKDDIVNA IEDAGFEGSL
210 220 230 240 250
VQSNQQDKLV LRVDGILNEL DAQVLEGILT RLNGVRQFRL DRISGELEVV
260 270 280 290 300
FDPEVVSSRS LVDGIEEDGF GKFKLRVMSP YERLSSKDTG EASNMFRRFI
310 320 330 340 350
SSLVLSIPLF FIQVICPHIA LFDALLVWRC GPFMMGDWLK WALVSVIQFV
360 370 380 390 400
IGKRFYVAAW RALRNGSTNM DVLVALGTSA SYFYSVGALL YGAVTGFWSP
410 420 430 440 450
TYFDASAMLI TFVLLGKYLE SLAKGKTSDA MKKLVQLTPA TAILLTEGKG
460 470 480 490 500
GKLVGEREID ALLIQPGDTL KVHPGAKIPA DGVVVWGSSY VNESMVTGES
510 520 530 540 550
VPVSKEVDSP VIGGTINMHG ALHMKATKVG SDAVLSQIIS LVETAQMSKA
560 570 580 590 600
PIQKFADYVA SIFVPVVITL ALFTLVGWSI GGAVGAYPDE WLPENGTHFV
610 620 630 640 650
FSLMFSISVV VIACPCALGL ATPTAVMVAT GVGATNGVLI KGGDALEKAH
660 670 680 690 700
KVKYVIFDKT GTLTQGKATV TTTKVFSEMD RGEFLTLVAS AEASSEHPLA
710 720 730 740 750
KAIVAYARHF HFFDESTEDG ETNNKDLQNS GWLLDTSDFS ALPGKGIQCL
760 770 780 790 800
VNEKMILVGN RKLMSENAIN IPDHVEKFVE DLEESGKTGV IVAYNGKLVG
810 820 830 840 850
VMGIADPLKR EAALVVEGLL RMGVRPIMVT GDNWRTARAV AKEVGIEDVR
860 870 880 890 900
AEVMPAGKAD VIRSLQKDGS TVAMVGDGIN DSPALAAADV GMAIGAGTDV
910 920 930 940 950
AIEAADYVLM RNNLEDVITA IDLSRKTLTR IRLNYVFAMA YNVVSIPIAA
960 970 980 990 1000
GVFFPVLRVQ LPPWAAGACM ALSSVSVVCS SLLLRRYKKP RLTTVLKITT

E
Length:1,001
Mass (Da):107,395
Last modified:May 1, 2000 - v1
Checksum:i7E820909C60D5B0F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF091112 Genomic DNA. Translation: AAD29115.1.
AF082565 mRNA. Translation: AAD29109.1.
AB016874 Genomic DNA. Translation: BAB08832.1.
AC002342 Genomic DNA. Translation: AAC79141.2.
CP002688 Genomic DNA. Translation: AED95163.1.
RefSeqiNP_199292.1. NM_123847.3.
UniGeneiAt.24482.

Genome annotation databases

EnsemblPlantsiAT5G44790.1; AT5G44790.1; AT5G44790.
GeneIDi834509.
GrameneiAT5G44790.1; AT5G44790.1; AT5G44790.
KEGGiath:AT5G44790.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF091112 Genomic DNA. Translation: AAD29115.1.
AF082565 mRNA. Translation: AAD29109.1.
AB016874 Genomic DNA. Translation: BAB08832.1.
AC002342 Genomic DNA. Translation: AAC79141.2.
CP002688 Genomic DNA. Translation: AED95163.1.
RefSeqiNP_199292.1. NM_123847.3.
UniGeneiAt.24482.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3DXSX-ray1.70X56-128[»]
ProteinModelPortaliQ9S7J8.
SMRiQ9S7J8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi19759. 3 interactors.
IntActiQ9S7J8. 2 interactors.
MINTiMINT-8066708.
STRINGi3702.AT5G44790.1.

Protein family/group databases

TCDBi3.A.3.5.32. the p-type atpase (p-atpase) superfamily.

PTM databases

SwissPalmiQ9S7J8.

Proteomic databases

PaxDbiQ9S7J8.
PRIDEiQ9S7J8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G44790.1; AT5G44790.1; AT5G44790.
GeneIDi834509.
GrameneiAT5G44790.1; AT5G44790.1; AT5G44790.
KEGGiath:AT5G44790.

Organism-specific databases

TAIRiAT5G44790.

Phylogenomic databases

eggNOGiKOG0207. Eukaryota.
COG2217. LUCA.
HOGENOMiHOG000250397.
InParanoidiQ9S7J8.
KOiK17686.
OMAiNEKMILV.
OrthoDBiEOG093602FP.
PhylomeDBiQ9S7J8.

Enzyme and pathway databases

BioCyciARA:AT5G44790-MONOMER.
ReactomeiR-ATH-936837. Ion transport by P-type ATPases.

Miscellaneous databases

EvolutionaryTraceiQ9S7J8.
PROiQ9S7J8.

Gene expression databases

GenevisibleiQ9S7J8. AT.

Family and domain databases

CDDicd00371. HMA. 2 hits.
Gene3Di2.70.150.10. 1 hit.
3.40.1110.10. 1 hit.
3.40.50.1000. 2 hits.
InterProiIPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR017969. Heavy-metal-associated_CS.
IPR006122. HMA_Cu_ion-bd.
IPR006121. HMA_dom.
IPR027256. P-typ_ATPase_IB.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00122. E1-E2_ATPase. 1 hit.
PF00403. HMA. 2 hits.
[Graphical view]
SUPFAMiSSF55008. SSF55008. 3 hits.
SSF56784. SSF56784. 2 hits.
TIGRFAMsiTIGR01525. ATPase-IB_hvy. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
TIGR00003. TIGR00003. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
PS01047. HMA_1. 2 hits.
PS50846. HMA_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHMA7_ARATH
AccessioniPrimary (citable) accession number: Q9S7J8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: May 1, 2000
Last modified: November 30, 2016
This is version 144 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.