Copper-transporting ATPase RAN1 - Arabidopsis thaliana (Mouse-ear cress)

Names and origin

Protein names

Recommended name:
    Copper-transporting ATPase RAN1
    EC=3.6.3.4
Alternative name(s):
    Protein RESPONSIVE TO ANTAGONIST 1

Gene names

Name:	RAN1
Ordered Locus Names:	At5g44790
ORF Names:	K23L20.14, T19K24.18

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

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Protein attributes

Sequence length	1001 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Involved in copper import into the cell. Essential for ethylene signaling, which requires copper. Acts by delivering copper to create functional hormone receptors.
Catalytic activity	ATP + H₂O + Cu²⁺(In) = ADP + phosphate + Cu²⁺(Out).
Subcellular location	Membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the cation transport ATPase (P-type) family. Type IB subfamily. Contains 3 HMA domains.

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Ontologies

Keywords
Biological process	Copper transport Ethylene signaling pathway Ion transport Transport
Cellular component	Membrane
Domain	Repeat Transmembrane
Ligand	ATP-binding Copper Magnesium Metal-binding Nucleotide-binding
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	ATP biosynthetic process Inferred from electronic annotation. Source: InterPro copper ion transport Inferred from electronic annotation. Source: UniProtKB-KW ethylene mediated signaling pathway Ref.1 Traceable author statement. Source: TAIR regulation of stomatal movement Inferred from mutant phenotype. Source: TAIR
Cellular component	Golgi apparatus Inferred from direct assay. Source: TAIR integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW copper ion binding Inferred from electronic annotation. Source: UniProtKB-KW copper-exporting ATPase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1001

1001

Copper-transporting ATPase RAN1

PRO_0000046402

Regions

Topological domain

1 – 298

298

Cytoplasmic Potential

Transmembrane

299 – 320

22

Potential

Topological domain

321 – 338

18

Extracellular Potential

Transmembrane

339 – 358

20

Potential

Topological domain

359 – 365

7

Cytoplasmic Potential

Transmembrane

366 – 386

21

Potential

Topological domain

387 – 403

17

Extracellular Potential

Transmembrane

404 – 424

21

Potential

Topological domain

425 – 558

134

Cytoplasmic Potential

Transmembrane

559 – 581

23

Potential

Topological domain

582 – 602

21

Extracellular Potential

Transmembrane

603 – 620

18

Potential

Topological domain

621 – 931

311

Cytoplasmic Potential

Transmembrane

932 – 951

20

Potential

Topological domain

952 – 963

12

Extracellular Potential

Transmembrane

964 – 982

19

Potential

Topological domain

983 – 1001

19

Cytoplasmic Potential

Domain

57 – 123

67

HMA 1

Domain

134 – 200

67

HMA 2

Domain

208 – 274

67

HMA 3; degenerate

Sites

Active site

658

1

4-aspartylphosphate intermediate By similarity

Metal binding

67

1

Copper Potential

Metal binding

70

1

Copper Potential

Metal binding

144

1

Copper Potential

Metal binding

147

1

Copper Potential

Metal binding

877

1

Magnesium By similarity

Metal binding

881

1

Magnesium By similarity

Amino acid modifications

Modified residue

598

1

Phosphohistidine By similarity

Experimental info

Mutagenesis

173

1

G → E in ran1-2.

Mutagenesis

497

1

T → I in ran1-1.

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Sequences

Sequence

Length

Mass (Da)

Tools

Q9S7J8-1 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 7E820909C60D5B0F
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FASTA

1,001

107,395

        10         20         30         40         50         60 
MAPSRRDLQL TPVTGGSSSQ ISDMEEVGLL DSYHNEANAD DILTKIEEGR DVSGLRKIQV 

        70         80         90        100        110        120 
GVTGMTCAAC SNSVEAALMN VNGVFKASVA LLQNRADVVF DPNLVKEEDI KEAIEDAGFE 

       130        140        150        160        170        180 
AEILAEEQTQ ATLVGQFTIG GMTCAACVNS VEGILRDLPG VKRAVVALST SLGEVEYDPN 

       190        200        210        220        230        240 
VINKDDIVNA IEDAGFEGSL VQSNQQDKLV LRVDGILNEL DAQVLEGILT RLNGVRQFRL 

       250        260        270        280        290        300 
DRISGELEVV FDPEVVSSRS LVDGIEEDGF GKFKLRVMSP YERLSSKDTG EASNMFRRFI 

       310        320        330        340        350        360 
SSLVLSIPLF FIQVICPHIA LFDALLVWRC GPFMMGDWLK WALVSVIQFV IGKRFYVAAW 

       370        380        390        400        410        420 
RALRNGSTNM DVLVALGTSA SYFYSVGALL YGAVTGFWSP TYFDASAMLI TFVLLGKYLE 

       430        440        450        460        470        480 
SLAKGKTSDA MKKLVQLTPA TAILLTEGKG GKLVGEREID ALLIQPGDTL KVHPGAKIPA 

       490        500        510        520        530        540 
DGVVVWGSSY VNESMVTGES VPVSKEVDSP VIGGTINMHG ALHMKATKVG SDAVLSQIIS 

       550        560        570        580        590        600 
LVETAQMSKA PIQKFADYVA SIFVPVVITL ALFTLVGWSI GGAVGAYPDE WLPENGTHFV 

       610        620        630        640        650        660 
FSLMFSISVV VIACPCALGL ATPTAVMVAT GVGATNGVLI KGGDALEKAH KVKYVIFDKT 

       670        680        690        700        710        720 
GTLTQGKATV TTTKVFSEMD RGEFLTLVAS AEASSEHPLA KAIVAYARHF HFFDESTEDG 

       730        740        750        760        770        780 
ETNNKDLQNS GWLLDTSDFS ALPGKGIQCL VNEKMILVGN RKLMSENAIN IPDHVEKFVE 

       790        800        810        820        830        840 
DLEESGKTGV IVAYNGKLVG VMGIADPLKR EAALVVEGLL RMGVRPIMVT GDNWRTARAV 

       850        860        870        880        890        900 
AKEVGIEDVR AEVMPAGKAD VIRSLQKDGS TVAMVGDGIN DSPALAAADV GMAIGAGTDV 

       910        920        930        940        950        960 
AIEAADYVLM RNNLEDVITA IDLSRKTLTR IRLNYVFAMA YNVVSIPIAA GVFFPVLRVQ 

       970        980        990       1000 
LPPWAAGACM ALSSVSVVCS SLLLRRYKKP RLTTVLKITT E

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Responsive-to-antagonist 1, a Menkes/Wilson disease-related copper transporter, is required for ethylene signaling in Arabidopsis." Hirayama T., Kieber J.J., Hirayama N., Kogan M., Guzman P., Nourizadeh S., Alonso J.M., Dailey W.P., Dancis A., Ecker J.R. Cell 97:383-393(1999) [PubMed: 10319818] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], MUTAGENESIS. Strain: cv. Columbia.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. VIII. Sequence features of the regions of 1,081,958 bp covered by seventeen physically assigned P1 and TAC clones." Asamizu E., Sato S., Kaneko T., Nakamura Y., Kotani H., Miyajima N., Tabata S. DNA Res. 5:379-391(1998) [PubMed: 10048488] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana." Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. Fransz P.F. Nature 408:823-826(2000) [PubMed: 11130714] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

AF091112 Genomic DNA. Translation: AAD29115.1.
AF082565 mRNA. Translation: AAD29109.1.
AB016874 Genomic DNA. Translation: BAB08832.1.
AC002342 Genomic DNA. Translation: AAC79141.2.

IPI

IPI00527068.

RefSeq

NP_199292.1.

UniGene

At.24482

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3DXS	X-ray	1.70	X	56-127	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

Q9S7J8. 2 interactions.

STRING

Q9S7J8.

Proteomic databases

PRIDE

Q9S7J8.

Genome annotation databases

GeneID

834509.

GenomeReviews

Gene locus AT5G44790 in contig BA000015_GR.

KEGG

ath:AT5G44790.

NMPDR

fig|3702.1.peg.26245.

Organism-specific databases

TAIR

At5g44790.

Phylogenomic databases

OMA

THGEPRV.

Enzyme and pathway databases

BRENDA

3.6.3.4. 302.

Gene expression databases

ArrayExpress

Q9S7J8.

Genevestigator

Q9S7J8.

GermOnline

AT5G44790. Arabidopsis thaliana.

Family and domain databases

InterPro

IPR001877. ATPase1_Cu-transp.
IPR008250. ATPase_P-typ_ATPase-assoc-reg.
IPR006403. ATPase_P-typ_cat/Cu-transptr.
IPR006416. ATPase_P-typ_heavy-metal.
IPR001757. ATPase_P-typ_ion-transptr.
IPR018303. ATPase_P-typ_P_site.
IPR006122. Cu_ion_bd.
IPR005834. Dehalogen-like_hydro.
IPR017969. Heavy-metal-associated_CS.
IPR006121. HeavyMe_transpt.
[Graphical view]

PANTHER

PTHR11939. ATPase_P. 1 hit.

Pfam

PF00122. E1-E2_ATPase. 1 hit.
PF00403. HMA. 2 hits.
PF00702. Hydrolase. 1 hit.
[Graphical view]

PRINTS

PR00119. CATATPASE.
PR00942. CUATPASEI.

TIGRFAMs

TIGR01511. ATPase-IB1_Cu. 1 hit.
TIGR01525. ATPase-IB_hvy. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
TIGR00003. Cu_ion_bd. 1 hit.

PROSITE

PS00154. ATPASE_E1_E2. 1 hit.
PS01047. HMA_1. 2 hits.
PS50846. HMA_2. 3 hits.
[Graphical view]

ProtoNet

Search...

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Entry information

Entry name

AHM5_ARATH

Accession

Primary (citable) accession number: Q9S7J8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	May 1, 2000
Last modified:	November 3, 2009
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)

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Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families