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Q9S7G6

- PNP2_ARATH

UniProt

Q9S7G6 - PNP2_ARATH

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Protein

Polyribonucleotide nucleotidyltransferase 2, mitochondrial

Gene
PNP2, At5g14580, T15N1.70
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Involved in the 3'-end maturation of mitochondrial mRNAs, rRNAs and tRNAs. Functions as a poly(A) mRNA 3'-5' degrading phosphorylase and is required for the degradation of highly expressed transcripts of non-coding regions.2 Publications

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: UniProtKB
  2. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB
  3. RNA binding Source: UniProtKB-KW

GO - Biological processi

  1. mitochondrial RNA catabolic process Source: UniProtKB
  2. mitochondrial RNA processing Source: UniProtKB
  3. mRNA catabolic process Source: InterPro
  4. mRNA processing Source: UniProtKB-KW
  5. RNA phosphodiester bond hydrolysis, exonucleolytic Source: GOC
  6. rRNA processing Source: UniProtKB-KW
  7. tRNA processing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Exonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase

Keywords - Biological processi

mRNA processing, rRNA processing, tRNA processing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciARA:AT5G14580-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferase 2, mitochondrial (EC:2.7.7.8)
Short name:
AtmtPNPase
Alternative name(s):
Polynucleotide phosphorylase 2
Short name:
PNPase 2
Gene namesi
Name:PNP2
Ordered Locus Names:At5g14580
ORF Names:T15N1.70
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G14580.

Subcellular locationi

Mitochondrion 1 Publication

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3939Mitochondrion Reviewed predictionAdd
BLAST
Chaini40 – 991952Polyribonucleotide nucleotidyltransferase 2, mitochondrialUniRule annotationPRO_5001028364Add
BLAST

Proteomic databases

PRIDEiQ9S7G6.

Expressioni

Gene expression databases

ArrayExpressiQ9S7G6.
GenevestigatoriQ9S7G6.

Structurei

3D structure databases

ProteinModelPortaliQ9S7G6.
SMRiQ9S7G6. Positions 50-670, 674-746.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini609 – 66759KHAdd
BLAST
Domaini678 – 74669S1 motif 1Add
BLAST
Domaini925 – 98763S1 motif 2Add
BLAST

Sequence similaritiesi

Contains 1 KH domain.
Contains 2 S1 motif domains.

Keywords - Domaini

Repeat, Transit peptide

Phylogenomic databases

HOGENOMiHOG000218327.
InParanoidiQ9S7G6.
KOiK00962.
OMAiGTENTIC.
PhylomeDBiQ9S7G6.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 2 hits.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9S7G6-1 [UniParc]FASTAAdd to Basket

« Hide

MSSIVNRASS ASLPNFLAWR ALGFRTICSG RLGFAPSVPD SPVSAGTKIL    50
ESFKEEFEVG SRVVSFETGK IARFANGSVV LGMDETKVLS TVTCAKTDSP 100
RDFLPLTVDY QEKQYAQGLI PNTYMRREGA PKERELLCGR LIDRPIRPLF 150
PTGFYHEVQI MASVLSSDGK QDPDILAANA SSAALMLSDV PWGGPIGVIR 200
IGRICGQFVV NPTMDELSSS DLNLIYACTR DKTMMIDVQS REISEKDLAA 250
ALRLAHPEAV KYLDPQIRLA EKAGKQKKEY KLSMLSDKTL EKVADLAATR 300
IESVFTDPSY GKFERGEALD NIGKDVRKVF EEEGDQESLS ILPKAVDTVR 350
KKVVRSRMIS DGFRVDGRHV DEVRPIYCES HYLPALHGSA LFSRGDTQVL 400
CTVTLGAPAE AQSLDSLVGP PKKRFMLHYS FPPYCTNEVG KRGGLNRREV 450
GHGTLAEKAL LAVLPPEEAF PYTIRINSEV MSSDGSTSMA SVCGGSMALM 500
DAGIPLRAHV AGVSVGLITD VDPSSGEIKD YRIVTDILGL EDHLGDMDFK 550
IAGTRDGVTA IQLDIKPAGI PLDIVCESLE NAREARLQIL DHMERNINSP 600
RGQDGAYSPR LATLKYSNDS LRTLIGPMGV LKRKIEVETG ARLSIDNGTL 650
TIVAKNQDVM EKAQEQVDFI IGRELVVGGV YKGTVSSIKE YGAFVEFPGG 700
QQGLLHMSEL SHEPVSKVSD VLDIGQCITT MCIETDVRGN IKLSRKALLP 750
KPKRKPASDA GKDPVMKESS TVYIENSSVG EIVASMPSIV TPLQKSRLSV 800
PAVVIRTAVE CNEAEKSSPV NDNDKPRRAA TSKPDRKPKS TASKLIATQK 850
EEEALESIAP EETSAECGEI LKQDGKLKSV SPKNNSTASN LVSFSKAKKS 900
TMKENLSENK AEESASVSTR KLKIGTEMTA TVDHVRALGL VLDLGGEIRG 950
MYIFQGDKDK FKKGDTLRVK CTSFNTKGVP VMALVDEEGE E 991
Length:991
Mass (Da):107,772
Last modified:May 1, 2000 - v1
Checksum:i9557E25980C6D1A5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y14685 mRNA. Translation: CAB43864.1.
Y14686 Genomic DNA. Translation: CAB43865.1.
AL163792 Genomic DNA. Translation: CAB87625.1.
CP002688 Genomic DNA. Translation: AED92050.1.
PIRiT48631.
RefSeqiNP_196962.1. NM_121462.3.
UniGeneiAt.427.

Genome annotation databases

EnsemblPlantsiAT5G14580.1; AT5G14580.1; AT5G14580.
GeneIDi831309.
KEGGiath:AT5G14580.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y14685 mRNA. Translation: CAB43864.1 .
Y14686 Genomic DNA. Translation: CAB43865.1 .
AL163792 Genomic DNA. Translation: CAB87625.1 .
CP002688 Genomic DNA. Translation: AED92050.1 .
PIRi T48631.
RefSeqi NP_196962.1. NM_121462.3.
UniGenei At.427.

3D structure databases

ProteinModelPortali Q9S7G6.
SMRi Q9S7G6. Positions 50-670, 674-746.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi Q9S7G6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G14580.1 ; AT5G14580.1 ; AT5G14580 .
GeneIDi 831309.
KEGGi ath:AT5G14580.

Organism-specific databases

GeneFarmi 4118.
TAIRi AT5G14580.

Phylogenomic databases

HOGENOMi HOG000218327.
InParanoidi Q9S7G6.
KOi K00962.
OMAi GTENTIC.
PhylomeDBi Q9S7G6.

Enzyme and pathway databases

BioCyci ARA:AT5G14580-MONOMER.

Miscellaneous databases

PROi Q9S7G6.

Gene expression databases

ArrayExpressi Q9S7G6.
Genevestigatori Q9S7G6.

Family and domain databases

Gene3Di 1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPi MF_01595. PNPase.
InterProi IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view ]
PANTHERi PTHR11252. PTHR11252. 1 hit.
Pfami PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view ]
SMARTi SM00322. KH. 1 hit.
SM00316. S1. 2 hits.
[Graphical view ]
SUPFAMi SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
PROSITEi PS50126. S1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Two exoribonucleases act sequentially to process mature 3'-ends of atp9 mRNAs in Arabidopsis mitochondria."
    Perrin R., Meyer E.H., Zaepfel M., Kim Y.-J., Mache R., Grienenberger J.-M., Gualberto J.M., Gagliardi D.
    J. Biol. Chem. 279:25440-25446(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, SUBCELLULAR LOCATION.
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
    Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
    , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
    Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Relaxed transcription in Arabidopsis mitochondria is counterbalanced by RNA stability control mediated by polyadenylation and polynucleotide phosphorylase."
    Holec S., Lange H., Kuehn K., Alioua M., Boerner T., Gagliardi D.
    Mol. Cell. Biol. 26:2869-2876(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiPNP2_ARATH
AccessioniPrimary (citable) accession number: Q9S7G6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2012
Last sequence update: May 1, 2000
Last modified: June 11, 2014
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Plants silencing PNP2 stop growing after two to three weeks (1 Publication).

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi