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Q9S7G6

- PNP2_ARATH

UniProt

Q9S7G6 - PNP2_ARATH

Protein

Polyribonucleotide nucleotidyltransferase 2, mitochondrial

Gene

PNP2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    Involved in the 3'-end maturation of mitochondrial mRNAs, rRNAs and tRNAs. Functions as a poly(A) mRNA 3'-5' degrading phosphorylase and is required for the degradation of highly expressed transcripts of non-coding regions.2 Publications

    Catalytic activityi

    RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.

    GO - Molecular functioni

    1. 3'-5'-exoribonuclease activity Source: UniProtKB
    2. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB
    3. RNA binding Source: UniProtKB-KW

    GO - Biological processi

    1. mitochondrial RNA catabolic process Source: UniProtKB
    2. mitochondrial RNA processing Source: UniProtKB
    3. mRNA catabolic process Source: InterPro
    4. mRNA processing Source: UniProtKB-KW
    5. RNA phosphodiester bond hydrolysis, exonucleolytic Source: GOC
    6. rRNA processing Source: UniProtKB-KW
    7. tRNA processing Source: UniProtKB-KW

    Keywords - Molecular functioni

    Exonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    mRNA processing, rRNA processing, tRNA processing

    Keywords - Ligandi

    RNA-binding

    Enzyme and pathway databases

    BioCyciARA:AT5G14580-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polyribonucleotide nucleotidyltransferase 2, mitochondrial (EC:2.7.7.8)
    Short name:
    AtmtPNPase
    Alternative name(s):
    Polynucleotide phosphorylase 2
    Short name:
    PNPase 2
    Gene namesi
    Name:PNP2
    Ordered Locus Names:At5g14580
    ORF Names:T15N1.70
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G14580.

    Subcellular locationi

    Mitochondrion 1 Publication

    GO - Cellular componenti

    1. mitochondrion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3939MitochondrionSequence AnalysisAdd
    BLAST
    Chaini40 – 991952Polyribonucleotide nucleotidyltransferase 2, mitochondrialPRO_5001028364Add
    BLAST

    Proteomic databases

    PRIDEiQ9S7G6.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9S7G6.
    GenevestigatoriQ9S7G6.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9S7G6.
    SMRiQ9S7G6. Positions 50-670, 674-746.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini609 – 66759KHAdd
    BLAST
    Domaini678 – 74669S1 motif 1Add
    BLAST
    Domaini925 – 98763S1 motif 2Add
    BLAST

    Sequence similaritiesi

    Contains 1 KH domain.Curated
    Contains 2 S1 motif domains.Curated

    Keywords - Domaini

    Repeat, Transit peptide

    Phylogenomic databases

    HOGENOMiHOG000218327.
    InParanoidiQ9S7G6.
    KOiK00962.
    OMAiGTENTIC.
    PhylomeDBiQ9S7G6.

    Family and domain databases

    Gene3Di1.10.10.400. 1 hit.
    2.40.50.140. 1 hit.
    3.30.1370.10. 1 hit.
    3.30.230.70. 2 hits.
    HAMAPiMF_01595. PNPase.
    InterProiIPR001247. ExoRNase_PH_dom1.
    IPR015847. ExoRNase_PH_dom2.
    IPR004087. KH_dom.
    IPR004088. KH_dom_type_1.
    IPR012340. NA-bd_OB-fold.
    IPR012162. PNPase.
    IPR027408. PNPase/RNase_PH_dom.
    IPR015848. PNPase_PH_RNA-bd_bac/org-type.
    IPR003029. Rbsml_prot_S1_RNA-bd_dom.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR022967. RNA-binding_domain_S1.
    [Graphical view]
    PANTHERiPTHR11252. PTHR11252. 1 hit.
    PfamiPF03726. PNPase. 1 hit.
    PF01138. RNase_PH. 2 hits.
    PF03725. RNase_PH_C. 2 hits.
    PF00575. S1. 1 hit.
    [Graphical view]
    SMARTiSM00322. KH. 1 hit.
    SM00316. S1. 2 hits.
    [Graphical view]
    SUPFAMiSSF50249. SSF50249. 1 hit.
    SSF54211. SSF54211. 2 hits.
    SSF54791. SSF54791. 1 hit.
    SSF55666. SSF55666. 2 hits.
    TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
    PROSITEiPS50126. S1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9S7G6-1 [UniParc]FASTAAdd to Basket

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    MSSIVNRASS ASLPNFLAWR ALGFRTICSG RLGFAPSVPD SPVSAGTKIL    50
    ESFKEEFEVG SRVVSFETGK IARFANGSVV LGMDETKVLS TVTCAKTDSP 100
    RDFLPLTVDY QEKQYAQGLI PNTYMRREGA PKERELLCGR LIDRPIRPLF 150
    PTGFYHEVQI MASVLSSDGK QDPDILAANA SSAALMLSDV PWGGPIGVIR 200
    IGRICGQFVV NPTMDELSSS DLNLIYACTR DKTMMIDVQS REISEKDLAA 250
    ALRLAHPEAV KYLDPQIRLA EKAGKQKKEY KLSMLSDKTL EKVADLAATR 300
    IESVFTDPSY GKFERGEALD NIGKDVRKVF EEEGDQESLS ILPKAVDTVR 350
    KKVVRSRMIS DGFRVDGRHV DEVRPIYCES HYLPALHGSA LFSRGDTQVL 400
    CTVTLGAPAE AQSLDSLVGP PKKRFMLHYS FPPYCTNEVG KRGGLNRREV 450
    GHGTLAEKAL LAVLPPEEAF PYTIRINSEV MSSDGSTSMA SVCGGSMALM 500
    DAGIPLRAHV AGVSVGLITD VDPSSGEIKD YRIVTDILGL EDHLGDMDFK 550
    IAGTRDGVTA IQLDIKPAGI PLDIVCESLE NAREARLQIL DHMERNINSP 600
    RGQDGAYSPR LATLKYSNDS LRTLIGPMGV LKRKIEVETG ARLSIDNGTL 650
    TIVAKNQDVM EKAQEQVDFI IGRELVVGGV YKGTVSSIKE YGAFVEFPGG 700
    QQGLLHMSEL SHEPVSKVSD VLDIGQCITT MCIETDVRGN IKLSRKALLP 750
    KPKRKPASDA GKDPVMKESS TVYIENSSVG EIVASMPSIV TPLQKSRLSV 800
    PAVVIRTAVE CNEAEKSSPV NDNDKPRRAA TSKPDRKPKS TASKLIATQK 850
    EEEALESIAP EETSAECGEI LKQDGKLKSV SPKNNSTASN LVSFSKAKKS 900
    TMKENLSENK AEESASVSTR KLKIGTEMTA TVDHVRALGL VLDLGGEIRG 950
    MYIFQGDKDK FKKGDTLRVK CTSFNTKGVP VMALVDEEGE E 991
    Length:991
    Mass (Da):107,772
    Last modified:May 1, 2000 - v1
    Checksum:i9557E25980C6D1A5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y14685 mRNA. Translation: CAB43864.1.
    Y14686 Genomic DNA. Translation: CAB43865.1.
    AL163792 Genomic DNA. Translation: CAB87625.1.
    CP002688 Genomic DNA. Translation: AED92050.1.
    PIRiT48631.
    RefSeqiNP_196962.1. NM_121462.3.
    UniGeneiAt.427.

    Genome annotation databases

    EnsemblPlantsiAT5G14580.1; AT5G14580.1; AT5G14580.
    GeneIDi831309.
    KEGGiath:AT5G14580.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y14685 mRNA. Translation: CAB43864.1 .
    Y14686 Genomic DNA. Translation: CAB43865.1 .
    AL163792 Genomic DNA. Translation: CAB87625.1 .
    CP002688 Genomic DNA. Translation: AED92050.1 .
    PIRi T48631.
    RefSeqi NP_196962.1. NM_121462.3.
    UniGenei At.427.

    3D structure databases

    ProteinModelPortali Q9S7G6.
    SMRi Q9S7G6. Positions 50-670, 674-746.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q9S7G6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G14580.1 ; AT5G14580.1 ; AT5G14580 .
    GeneIDi 831309.
    KEGGi ath:AT5G14580.

    Organism-specific databases

    GeneFarmi 4118.
    TAIRi AT5G14580.

    Phylogenomic databases

    HOGENOMi HOG000218327.
    InParanoidi Q9S7G6.
    KOi K00962.
    OMAi GTENTIC.
    PhylomeDBi Q9S7G6.

    Enzyme and pathway databases

    BioCyci ARA:AT5G14580-MONOMER.

    Miscellaneous databases

    PROi Q9S7G6.

    Gene expression databases

    ArrayExpressi Q9S7G6.
    Genevestigatori Q9S7G6.

    Family and domain databases

    Gene3Di 1.10.10.400. 1 hit.
    2.40.50.140. 1 hit.
    3.30.1370.10. 1 hit.
    3.30.230.70. 2 hits.
    HAMAPi MF_01595. PNPase.
    InterProi IPR001247. ExoRNase_PH_dom1.
    IPR015847. ExoRNase_PH_dom2.
    IPR004087. KH_dom.
    IPR004088. KH_dom_type_1.
    IPR012340. NA-bd_OB-fold.
    IPR012162. PNPase.
    IPR027408. PNPase/RNase_PH_dom.
    IPR015848. PNPase_PH_RNA-bd_bac/org-type.
    IPR003029. Rbsml_prot_S1_RNA-bd_dom.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR022967. RNA-binding_domain_S1.
    [Graphical view ]
    PANTHERi PTHR11252. PTHR11252. 1 hit.
    Pfami PF03726. PNPase. 1 hit.
    PF01138. RNase_PH. 2 hits.
    PF03725. RNase_PH_C. 2 hits.
    PF00575. S1. 1 hit.
    [Graphical view ]
    SMARTi SM00322. KH. 1 hit.
    SM00316. S1. 2 hits.
    [Graphical view ]
    SUPFAMi SSF50249. SSF50249. 1 hit.
    SSF54211. SSF54211. 2 hits.
    SSF54791. SSF54791. 1 hit.
    SSF55666. SSF55666. 2 hits.
    TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
    PROSITEi PS50126. S1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Two exoribonucleases act sequentially to process mature 3'-ends of atp9 mRNAs in Arabidopsis mitochondria."
      Perrin R., Meyer E.H., Zaepfel M., Kim Y.-J., Mache R., Grienenberger J.-M., Gualberto J.M., Gagliardi D.
      J. Biol. Chem. 279:25440-25446(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, SUBCELLULAR LOCATION.
      Strain: cv. Columbia.
    2. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
      Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
      , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
      Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Relaxed transcription in Arabidopsis mitochondria is counterbalanced by RNA stability control mediated by polyadenylation and polynucleotide phosphorylase."
      Holec S., Lange H., Kuehn K., Alioua M., Boerner T., Gagliardi D.
      Mol. Cell. Biol. 26:2869-2876(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiPNP2_ARATH
    AccessioniPrimary (citable) accession number: Q9S7G6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 28, 2012
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 107 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Plants silencing PNP2 stop growing after two to three weeks.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3