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Q9S7E9

- GGT2_ARATH

UniProt

Q9S7E9 - GGT2_ARATH

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Protein

Glutamate--glyoxylate aminotransferase 2

Gene

GGAT2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the Glu:glyoxylate aminotransferase (GGT), Ala:glyoxylate aminotransferase (AGT), Ala:2-oxoglutarate aminotransferase (AKT) and Glu:pyruvate aminotransferase (GPT) reactions in peroxisomes.1 Publication

Catalytic activityi

L-alanine + 2-oxoglutarate = pyruvate + L-glutamate.1 Publication
L-alanine + glyoxylate = pyruvate + glycine.1 Publication
Glycine + 2-oxoglutarate = glyoxylate + L-glutamate.1 Publication

Cofactori

Kineticsi

  1. KM=3.32 mM for glutamate1 Publication
  2. KM=3.56 mM for alanine1 Publication
  3. KM=0.14 mM for glyoxylate1 Publication
  4. KM=0.51 mM for 2-oxoglutarate1 Publication
  5. KM=0.36 mM for pyruvate1 Publication

Pathwayi

GO - Molecular functioni

  1. alanine-glyoxylate transaminase activity Source: TAIR
  2. glycine:2-oxoglutarate aminotransferase activity Source: TAIR
  3. L-alanine:2-oxoglutarate aminotransferase activity Source: TAIR
  4. pyridoxal phosphate binding Source: InterPro

GO - Biological processi

  1. biosynthetic process Source: InterPro
  2. L-alanine catabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciARA:GQT-2823-MONOMER.
ARA:GQT-2824-MONOMER.
ARA:GQT-2825-MONOMER.
MetaCyc:AT1G70580-MONOMER.
BRENDAi2.6.1.44. 302.
ReactomeiREACT_232680. Amino acid synthesis and interconversion (transamination).
SABIO-RKQ9S7E9.
UniPathwayiUPA00322.
UPA00528; UER00586.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--glyoxylate aminotransferase 2 (EC:2.6.1.4)
Short name:
AtGGT2
Alternative name(s):
Alanine aminotransferase GGT2 (EC:2.6.1.2)
Alanine--glyoxylate aminotransferase GGT2 (EC:2.6.1.44)
Alanine-2-oxoglutarate aminotransferase 2 (EC:2.6.1.-)
Gene namesi
Name:GGAT2
Synonyms:AOAT2, GGT2
Ordered Locus Names:At1g70580
ORF Names:F24J13.15, F5A18.24
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G70580.

Subcellular locationi

Peroxisome 3 Publications

GO - Cellular componenti

  1. chloroplast Source: TAIR
  2. chloroplast stroma Source: TAIR
  3. peroxisome Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 481481Glutamate--glyoxylate aminotransferase 2PRO_0000416041Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei291 – 2911N6-(pyridoxal phosphate)lysineBy similarity

Post-translational modificationi

The N-terminus is blocked.By similarity

Proteomic databases

PRIDEiQ9S7E9.
ProMEXiQ9S7E9.

Expressioni

Tissue specificityi

Expressed at low levels in seedlings, leaves, flowers, roots, and green siliques.2 Publications

Gene expression databases

GenevestigatoriQ9S7E9.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi3702.AT1G70580.3-P.

Structurei

3D structure databases

ProteinModelPortaliQ9S7E9.
SMRiQ9S7E9. Positions 9-472.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi479 – 4813Peroxisomal targeting signal

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000215020.
InParanoidiQ9S7E9.
KOiK14272.
OMAiLNEEKCW.
PhylomeDBiQ9S7E9.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9S7E9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSLKALDYES LNENVKNCQY AVRGELYLRA SELQKEGKKI IFTNVGNPHA
60 70 80 90 100
LGQKPLTFPR QVVSLCQAPF LLDDPNVGMI FPADAIARAK HYLSLTSGGL
110 120 130 140 150
GAYSDSRGLP GVRKEVAEFI ERRDGYPSDP ELIFLTDGAS KGVMQILNCV
160 170 180 190 200
IRGQKDGILV PVPQYPLYSA TISLLGGTLV PYYLEESENW GLDVNNLRQS
210 220 230 240 250
VAQARSQGIT VRAMVIINPG NPTGQCLSEA NIREILRFCC DERLVLLGDE
260 270 280 290 300
VYQQNIYQDE RPFISSKKVL MDMGAPISKE VQLISFHTVS KGYWGECGQR
310 320 330 340 350
GGYFEMTNIP PRTVEEIYKV ASIALSPNVS AQIFMGLMVS PPKPGDISYD
360 370 380 390 400
QFVRESKGIL ESLRRRARMM TDGFNSCKNV VCNFTEGAMY SFPQIKLPSK
410 420 430 440 450
AIQAAKQAGK VPDVFYCLKL LEATGISTVP GSGFGQKEGV FHLRTTILPA
460 470 480
EEEMPEIMDS FKKFNDEFMS QYADNFGYSR M
Length:481
Mass (Da):53,444
Last modified:May 1, 2000 - v1
Checksum:iA00D1D49157021FA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti37 – 371G → C in AAM61453. 1 PublicationCurated
Sequence conflicti118 – 1181E → D in AAM61453. 1 PublicationCurated
Sequence conflicti318 – 3181Y → H in BAH20108. (PubMed:19423640)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF479640 mRNA. Translation: AAN62333.1.
AC010796 Genomic DNA. Translation: AAG52480.1.
AC011663 Genomic DNA. Translation: AAG52344.1.
CP002684 Genomic DNA. Translation: AEE35082.1.
CP002684 Genomic DNA. Translation: AEE35083.1.
CP002684 Genomic DNA. Translation: AEE35084.1.
CP002684 Genomic DNA. Translation: AEE35085.1.
AY035130 mRNA. Translation: AAK59635.1.
AY062982 mRNA. Translation: AAL34156.1.
AK316788 mRNA. Translation: BAH19506.1.
AK317441 mRNA. Translation: BAH20108.1.
AY084890 mRNA. Translation: AAM61453.1.
PIRiH96729.
RefSeqiNP_001031262.1. NM_001036185.1.
NP_001031263.1. NM_001036186.1.
NP_177215.1. NM_105726.2.
NP_974122.1. NM_202393.1.
UniGeneiAt.18115.

Genome annotation databases

EnsemblPlantsiAT1G70580.1; AT1G70580.1; AT1G70580.
AT1G70580.2; AT1G70580.2; AT1G70580.
AT1G70580.3; AT1G70580.3; AT1G70580.
AT1G70580.4; AT1G70580.4; AT1G70580.
GeneIDi843395.
KEGGiath:AT1G70580.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF479640 mRNA. Translation: AAN62333.1 .
AC010796 Genomic DNA. Translation: AAG52480.1 .
AC011663 Genomic DNA. Translation: AAG52344.1 .
CP002684 Genomic DNA. Translation: AEE35082.1 .
CP002684 Genomic DNA. Translation: AEE35083.1 .
CP002684 Genomic DNA. Translation: AEE35084.1 .
CP002684 Genomic DNA. Translation: AEE35085.1 .
AY035130 mRNA. Translation: AAK59635.1 .
AY062982 mRNA. Translation: AAL34156.1 .
AK316788 mRNA. Translation: BAH19506.1 .
AK317441 mRNA. Translation: BAH20108.1 .
AY084890 mRNA. Translation: AAM61453.1 .
PIRi H96729.
RefSeqi NP_001031262.1. NM_001036185.1.
NP_001031263.1. NM_001036186.1.
NP_177215.1. NM_105726.2.
NP_974122.1. NM_202393.1.
UniGenei At.18115.

3D structure databases

ProteinModelPortali Q9S7E9.
SMRi Q9S7E9. Positions 9-472.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT1G70580.3-P.

Proteomic databases

PRIDEi Q9S7E9.
ProMEXi Q9S7E9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G70580.1 ; AT1G70580.1 ; AT1G70580 .
AT1G70580.2 ; AT1G70580.2 ; AT1G70580 .
AT1G70580.3 ; AT1G70580.3 ; AT1G70580 .
AT1G70580.4 ; AT1G70580.4 ; AT1G70580 .
GeneIDi 843395.
KEGGi ath:AT1G70580.

Organism-specific databases

TAIRi AT1G70580.

Phylogenomic databases

HOGENOMi HOG000215020.
InParanoidi Q9S7E9.
KOi K14272.
OMAi LNEEKCW.
PhylomeDBi Q9S7E9.

Enzyme and pathway databases

UniPathwayi UPA00322 .
UPA00528 ; UER00586 .
BioCyci ARA:GQT-2823-MONOMER.
ARA:GQT-2824-MONOMER.
ARA:GQT-2825-MONOMER.
MetaCyc:AT1G70580-MONOMER.
BRENDAi 2.6.1.44. 302.
Reactomei REACT_232680. Amino acid synthesis and interconversion (transamination).
SABIO-RK Q9S7E9.

Gene expression databases

Genevestigatori Q9S7E9.

Family and domain databases

Gene3Di 3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProi IPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view ]
Pfami PF00155. Aminotran_1_2. 1 hit.
[Graphical view ]
SUPFAMi SSF53383. SSF53383. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Alanine aminotransferase homologs catalyze the glutamate:glyoxylate aminotransferase reaction in peroxisomes of Arabidopsis."
    Liepman A.H., Olsen L.J.
    Plant Physiol. 131:215-227(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs."
    Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K.
    DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
    Tissue: Root and Rosette leaf.
  6. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  7. "Proteome analysis of Arabidopsis leaf peroxisomes reveals novel targeting peptides, metabolic pathways, and defense mechanisms."
    Reumann S., Babujee L., Ma C., Wienkoop S., Siemsen T., Antonicelli G.E., Rasche N., Lueder F., Weckwerth W., Jahn O.
    Plant Cell 19:3170-3193(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 91-107 AND 213-233, SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  8. "Proteomic analysis of leaf peroxisomal proteins in greening cotyledons of Arabidopsis thaliana."
    Fukao Y., Hayashi M., Nishimura M.
    Plant Cell Physiol. 43:689-696(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  9. "Identification of photorespiratory glutamate:glyoxylate aminotransferase (GGAT) gene in Arabidopsis."
    Igarashi D., Miwa T., Seki M., Kobayashi M., Kato T., Tabata S., Shinozaki K., Ohsumi C.
    Plant J. 33:975-987(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiGGT2_ARATH
AccessioniPrimary (citable) accession number: Q9S7E9
Secondary accession number(s): B9DH91, Q8LFE9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 21, 2012
Last sequence update: May 1, 2000
Last modified: November 26, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3