Reviewed, UniProtKB/Swiss-Prot Q9S7E4 (FDH_ARATH)

Names and origin

Protein names

Recommended name:
    Formate dehydrogenase, mitochondrial
    EC=1.2.1.2
Alternative name(s):
    NAD-dependent formate dehydrogenase
      Short name=FDH

Gene names

Name:	FDH1
Synonyms:	FDH
Ordered Locus Names:	At5g14780
ORF Names:	T9L3_80

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	384 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Formate + NAD+ = CO2 + NADH.
Subcellular location	Mitochondrion Ref.6.
Sequence similarities	Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. FDH subfamily.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to cadmium ion Inferred from expression pattern. Source: TAIR
Cellular component	chloroplast Inferred from direct assay. Source: TAIR mitochondrion Ref.6 Inferred from direct assay. Source: TAIR thylakoid Inferred from direct assay. Source: TAIR
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro formate dehydrogenase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 27	27	Mitochondrion By similarity
	Chain	28 – 384	357	Formate dehydrogenase, mitochondrial		PRO_0000007193
Regions
	Nucleotide binding	207 – 208	2	NAD By similarity
	Nucleotide binding	288 – 290	3	NAD By similarity
	Nucleotide binding	338 – 341	4	NAD By similarity
Sites
	Active site	290	1	By similarity
	Active site	338	1	Proton donor By similarity
	Binding site	314	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9S7E4-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: A12BA423019D862B	FASTA	384	42,410
	10 20 30 40 50 60 MAMRQAAKAT IRACSSSSSS GYFARRQFNA SSGDSKKIVG VFYKANEYAT KNPNFLGCVE 70 80 90 100 110 120 NALGIRDWLE SQGHQYIVTD DKEGPDCELE KHIPDLHVLI STPFHPAYVT AERIKKAKNL 130 140 150 160 170 180 KLLLTAGIGS DHIDLQAAAA AGLTVAEVTG SNVVSVAEDE LMRILILMRN FVPGYNQVVK 190 200 210 220 230 240 GEWNVAGIAY RAYDLEGKTI GTVGAGRIGK LLLQRLKPFG CNLLYHDRLQ MAPELEKETG 250 260 270 280 290 300 AKFVEDLNEM LPKCDVIVIN MPLTEKTRGM FNKELIGKLK KGVLIVNNAR GAIMERQAVV 310 320 330 340 350 360 DAVESGHIGG YSGDVWDPQP APKDHPWRYM PNQAMTPHTS GTTIDAQLRY AAGTKDMLER 370 380 YFKGEDFPTE NYIVKDGELA PQYR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Formate dehydrogenase cDNA from Arabidopsis thaliana." Nishikawa T., Fukusaki E., Kobayashi A. Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Arabidopsis thaliana mRNA for NAD-dependent formate dehydrogenas 1." Li R., Bonham-Smith P.C., King J. Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Induction of leaf formate dehydrogenase by one-carbon metabolites." Markwell J., Osterman J.C., Olson B.J., Skavdahl M., Ramberg H., Germann M.C. Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana." Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F. Nature 408:823-826(2000) [PubMed: 11130714] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[5]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins." Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H. Plant Cell 16:241-256(2004) [PubMed: 14671022] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB023897 mRNA. Translation: BAA88683.1. AF208028 mRNA. Translation: AAF19435.1. AF208029 mRNA. Translation: AAF19436.1. AF217195 mRNA. Translation: AAF67100.1. AL391149 Genomic DNA. Translation: CAC01877.1. AY054285 mRNA. Translation: AAL06944.1. AY039609 mRNA. Translation: AAK62664.1. AY081734 mRNA. Translation: AAL87387.1.
IPI	IPI00534642.
PIR	T51423.
RefSeq	NP_196982.1.
UniGene	At.23637
3D structure databases
SMR	Q9S7E4. Positions 53-380.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9S7E4. 1 interaction.
STRING	Q9S7E4.
Proteomic databases
PRIDE	Q9S7E4.
Genome annotation databases
GeneID	831330.
GenomeReviews	Gene locus AT5G14780 in contig BA000015_GR.
KEGG	ath:AT5G14780.
NMPDR	fig|3702.1.peg.23616.
Organism-specific databases
TAIR	At5g14780.
Phylogenomic databases
HOGENOM	HBG328050.
InParanoid	Q9S7E4.
OMA	KANEYAT.
PhylomeDB	Q9S7E4.
Enzyme and pathway databases
BRENDA	1.2.1.2. 302.
Gene expression databases
ArrayExpress	Q9S7E4.
Genevestigator	Q9S7E4.
GermOnline	AT5G14780. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR006139. D-isomer_2_OHA_DH_cat_dom. IPR006140. D-isomer_2_OHA_DH_NAD-bd. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 2 hits.
Pfam	PF00389. 2-Hacid_dh. 1 hit. PF02826. 2-Hacid_dh_C. 1 hit. [Graphical view]
PROSITE	PS00065. D_2_HYDROXYACID_DH_1. 1 hit. PS00670. D_2_HYDROXYACID_DH_2. 1 hit. PS00671. D_2_HYDROXYACID_DH_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FDH_ARATH

Accession

Primary (citable) accession number: Q9S7E4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 27, 2002
Last sequence update:	May 1, 2000
Last modified:	February 9, 2010
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)