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Protein

Squamosa promoter-binding-like protein 4

Gene

SPL4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Trans-acting factor that binds specifically to the consensus nucleotide sequence 5'-TNCGTACAA-3' of AP1 promoter. Promotes both vegetative phase change and flowering.2 Publications

Cofactori

Zn2+Note: Binds 2 Zn2+ ions per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi54Zinc 11
Metal bindingi59Zinc 11
Metal bindingi76Zinc 11
Metal bindingi79Zinc 11
Metal bindingi95Zinc 21
Metal bindingi98Zinc 21
Metal bindingi102Zinc 21
Metal bindingi114Zinc 21

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri51 – 128SBP-typePROSITE-ProRule annotationAdd BLAST78

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • DNA binding transcription factor activity Source: TAIR
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • flower development Source: InterPro
  • regulation of transcription, DNA-templated Source: TAIR
  • regulation of vegetative phase change Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionDNA-binding
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Squamosa promoter-binding-like protein 4
Gene namesi
Name:SPL4
Ordered Locus Names:At1g53160
ORF Names:F12M16.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G53160
TAIRilocus:2009675 AT1G53160

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001327251 – 174Squamosa promoter-binding-like protein 4Add BLAST174

Proteomic databases

PaxDbiQ9S7A9
PRIDEiQ9S7A9

PTM databases

iPTMnetiQ9S7A9

Expressioni

Tissue specificityi

Expressed in the rib meristem and inter-primordial tissue of the inflorescence apex.1 Publication

Developmental stagei

Increases during floral transition and stay high thereafter.2 Publications

Inductioni

Negatively regulated by microRNAs miR156 and miR157.2 Publications

Gene expression databases

ExpressionAtlasiQ9S7A9 baseline and differential
GenevisibleiQ9S7A9 AT

Interactioni

Protein-protein interaction databases

BioGridi26974, 1 interactor
IntActiQ9S7A9, 4 interactors
STRINGi3702.AT1G53160.1

Structurei

Secondary structure

1174
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi68 – 72Combined sources5
Helixi77 – 80Combined sources4
Beta strandi85 – 87Combined sources3
Beta strandi90 – 94Combined sources5
Turni96 – 98Combined sources3
Beta strandi99 – 103Combined sources5
Turni104 – 106Combined sources3
Turni116 – 119Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1UL4NMR-A51-131[»]
ProteinModelPortaliQ9S7A9
SMRiQ9S7A9
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9S7A9

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi111 – 127Bipartite nuclear localization signalSequence analysisAdd BLAST17

Domaini

The SBP-type zinc finger is required for the binding to DNA.By similarity

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri51 – 128SBP-typePROSITE-ProRule annotationAdd BLAST78

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiENOG410J055 Eukaryota
ENOG410YKCI LUCA
HOGENOMiHOG000005763
InParanoidiQ9S7A9
OMAiMIQNQER
OrthoDBiEOG09360SQ8
PhylomeDBiQ9S7A9

Family and domain databases

Gene3Di4.10.1100.10, 1 hit
InterProiView protein in InterPro
IPR004333 SBP_dom
IPR017238 SBP_fam
IPR036893 SBP_sf
PfamiView protein in Pfam
PF03110 SBP, 1 hit
PIRSFiPIRSF037575 SBP, 1 hit
SUPFAMiSSF103612 SSF103612, 1 hit
PROSITEiView protein in PROSITE
PS51141 ZF_SBP, 1 hit

Sequencei

Sequence statusi: Complete.

Q9S7A9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGKRSQGQG YMKKKSYLVE EDMETDTDEE EEVGRDRVRG SRGSINRGGS
60 70 80 90 100
LRLCQVDRCT ADMKEAKLYH RRHKVCEVHA KASSVFLSGL NQRFCQQCSR
110 120 130 140 150
FHDLQEFDEA KRSCRRRLAG HNERRRKSSG ESTYGEGSGR RGINGQVVMQ
160 170
NQERSRVEMT LPMPNSSFKR PQIR
Length:174
Mass (Da):20,120
Last modified:May 1, 2000 - v1
Checksum:iB569272D389655AB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ011630 Genomic DNA Translation: CAB56582.1
AJ011631 mRNA Translation: CAB56583.1
AJ011632 mRNA Translation: CAB56584.1
AC008007 Genomic DNA Translation: AAF69527.1
CP002684 Genomic DNA Translation: AEE32896.1
CP002684 Genomic DNA Translation: AEE32897.1
BT002856 mRNA Translation: AAO22673.1
BT004391 mRNA Translation: AAO42385.1
AY084902 mRNA Translation: AAM61465.1
PIRiT52599
T52600
RefSeqiNP_175723.1, NM_104194.4
NP_974014.1, NM_202285.2
UniGeneiAt.496

Genome annotation databases

EnsemblPlantsiAT1G53160.1; AT1G53160.1; AT1G53160
AT1G53160.2; AT1G53160.2; AT1G53160
GeneIDi841749
GrameneiAT1G53160.1; AT1G53160.1; AT1G53160
AT1G53160.2; AT1G53160.2; AT1G53160
KEGGiath:AT1G53160

Similar proteinsi

Entry informationi

Entry nameiSPL4_ARATH
AccessioniPrimary (citable) accession number: Q9S7A9
Secondary accession number(s): Q9SMX8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: May 1, 2000
Last modified: May 23, 2018
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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