Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Squamosa promoter-binding-like protein 4

Gene

SPL4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Trans-acting factor that binds specifically to the consensus nucleotide sequence 5'-TNCGTACAA-3' of AP1 promoter. Promotes both vegetative phase change and flowering.2 Publications

Cofactori

Zn2+Note: Binds 2 Zn2+ ions per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi54Zinc 11
Metal bindingi59Zinc 11
Metal bindingi76Zinc 11
Metal bindingi79Zinc 11
Metal bindingi95Zinc 21
Metal bindingi98Zinc 21
Metal bindingi102Zinc 21
Metal bindingi114Zinc 21

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri51 – 128SBP-typePROSITE-ProRule annotationAdd BLAST78

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • transcription factor activity, sequence-specific DNA binding Source: TAIR

GO - Biological processi

  • flower development Source: InterPro
  • regulation of transcription, DNA-templated Source: TAIR
  • regulation of vegetative phase change Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Squamosa promoter-binding-like protein 4
Gene namesi
Name:SPL4
Ordered Locus Names:At1g53160
ORF Names:F12M16.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G53160.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001327251 – 174Squamosa promoter-binding-like protein 4Add BLAST174

Proteomic databases

PaxDbiQ9S7A9.

PTM databases

iPTMnetiQ9S7A9.

Expressioni

Tissue specificityi

Expressed in the rib meristem and inter-primordial tissue of the inflorescence apex.1 Publication

Developmental stagei

Increases during floral transition and stay high thereafter.2 Publications

Inductioni

Negatively regulated by microRNAs miR156 and miR157.2 Publications

Gene expression databases

GenevisibleiQ9S7A9. AT.

Interactioni

Protein-protein interaction databases

BioGridi26974. 1 interactor.
STRINGi3702.AT1G53160.1.

Structurei

Secondary structure

1174
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi68 – 72Combined sources5
Helixi77 – 80Combined sources4
Beta strandi85 – 87Combined sources3
Beta strandi90 – 94Combined sources5
Turni96 – 98Combined sources3
Beta strandi99 – 103Combined sources5
Turni104 – 106Combined sources3
Turni116 – 119Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1UL4NMR-A51-131[»]
ProteinModelPortaliQ9S7A9.
SMRiQ9S7A9.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9S7A9.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi111 – 127Bipartite nuclear localization signalSequence analysisAdd BLAST17

Domaini

The SBP-type zinc finger is required for the binding to DNA.By similarity

Sequence similaritiesi

Contains 1 SBP-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri51 – 128SBP-typePROSITE-ProRule annotationAdd BLAST78

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiENOG410J055. Eukaryota.
ENOG410YKCI. LUCA.
HOGENOMiHOG000005763.
InParanoidiQ9S7A9.
OMAiERSRVEM.
OrthoDBiEOG09360SQ8.
PhylomeDBiQ9S7A9.

Family and domain databases

Gene3Di4.10.1100.10. 1 hit.
InterProiIPR017238. SBP_fam.
IPR004333. Transcpt_factor_SBP-box.
[Graphical view]
PfamiPF03110. SBP. 1 hit.
[Graphical view]
PIRSFiPIRSF037575. SBP. 1 hit.
SUPFAMiSSF103612. SSF103612. 1 hit.
PROSITEiPS51141. ZF_SBP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9S7A9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGKRSQGQG YMKKKSYLVE EDMETDTDEE EEVGRDRVRG SRGSINRGGS
60 70 80 90 100
LRLCQVDRCT ADMKEAKLYH RRHKVCEVHA KASSVFLSGL NQRFCQQCSR
110 120 130 140 150
FHDLQEFDEA KRSCRRRLAG HNERRRKSSG ESTYGEGSGR RGINGQVVMQ
160 170
NQERSRVEMT LPMPNSSFKR PQIR
Length:174
Mass (Da):20,120
Last modified:May 1, 2000 - v1
Checksum:iB569272D389655AB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ011630 Genomic DNA. Translation: CAB56582.1.
AJ011631 mRNA. Translation: CAB56583.1.
AJ011632 mRNA. Translation: CAB56584.1.
AC008007 Genomic DNA. Translation: AAF69527.1.
CP002684 Genomic DNA. Translation: AEE32896.1.
CP002684 Genomic DNA. Translation: AEE32897.1.
BT002856 mRNA. Translation: AAO22673.1.
BT004391 mRNA. Translation: AAO42385.1.
AY084902 mRNA. Translation: AAM61465.1.
PIRiT52599.
T52600.
RefSeqiNP_175723.1. NM_104194.4.
NP_974014.1. NM_202285.2.
UniGeneiAt.496.

Genome annotation databases

EnsemblPlantsiAT1G53160.1; AT1G53160.1; AT1G53160.
AT1G53160.2; AT1G53160.2; AT1G53160.
GeneIDi841749.
GrameneiAT1G53160.1; AT1G53160.1; AT1G53160.
AT1G53160.2; AT1G53160.2; AT1G53160.
KEGGiath:AT1G53160.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ011630 Genomic DNA. Translation: CAB56582.1.
AJ011631 mRNA. Translation: CAB56583.1.
AJ011632 mRNA. Translation: CAB56584.1.
AC008007 Genomic DNA. Translation: AAF69527.1.
CP002684 Genomic DNA. Translation: AEE32896.1.
CP002684 Genomic DNA. Translation: AEE32897.1.
BT002856 mRNA. Translation: AAO22673.1.
BT004391 mRNA. Translation: AAO42385.1.
AY084902 mRNA. Translation: AAM61465.1.
PIRiT52599.
T52600.
RefSeqiNP_175723.1. NM_104194.4.
NP_974014.1. NM_202285.2.
UniGeneiAt.496.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1UL4NMR-A51-131[»]
ProteinModelPortaliQ9S7A9.
SMRiQ9S7A9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi26974. 1 interactor.
STRINGi3702.AT1G53160.1.

PTM databases

iPTMnetiQ9S7A9.

Proteomic databases

PaxDbiQ9S7A9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G53160.1; AT1G53160.1; AT1G53160.
AT1G53160.2; AT1G53160.2; AT1G53160.
GeneIDi841749.
GrameneiAT1G53160.1; AT1G53160.1; AT1G53160.
AT1G53160.2; AT1G53160.2; AT1G53160.
KEGGiath:AT1G53160.

Organism-specific databases

TAIRiAT1G53160.

Phylogenomic databases

eggNOGiENOG410J055. Eukaryota.
ENOG410YKCI. LUCA.
HOGENOMiHOG000005763.
InParanoidiQ9S7A9.
OMAiERSRVEM.
OrthoDBiEOG09360SQ8.
PhylomeDBiQ9S7A9.

Miscellaneous databases

EvolutionaryTraceiQ9S7A9.
PROiQ9S7A9.

Gene expression databases

GenevisibleiQ9S7A9. AT.

Family and domain databases

Gene3Di4.10.1100.10. 1 hit.
InterProiIPR017238. SBP_fam.
IPR004333. Transcpt_factor_SBP-box.
[Graphical view]
PfamiPF03110. SBP. 1 hit.
[Graphical view]
PIRSFiPIRSF037575. SBP. 1 hit.
SUPFAMiSSF103612. SSF103612. 1 hit.
PROSITEiPS51141. ZF_SBP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSPL4_ARATH
AccessioniPrimary (citable) accession number: Q9S7A9
Secondary accession number(s): Q9SMX8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: May 1, 2000
Last modified: November 30, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.