Reviewed, UniProtKB/Swiss-Prot Q9S7A0 (DHE3_ARATH)

Names and origin

Protein names

Recommended name:
    Probable glutamate dehydrogenase 3
      Short name=GDH 3
    EC=1.4.1.3

Gene names

Name:	GSH3
Ordered Locus Names:	At3g03910
ORF Names:	T11I18.2, F20H23.4

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	411 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	L-glutamate + H2O + NAD(P)+ = 2-oxoglutarate + NH3 + NAD(P)H.
Sequence similarities	Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW regulation of nitrogen compound metabolic process Inferred from expression pattern. Source: TAIR
Cellular component	mitochondrion Inferred from direct assay. Source: TAIR
Molecular function	binding Inferred from electronic annotation. Source: InterPro glutamate dehydrogenase [NAD(P)+] activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 411	411	Probable glutamate dehydrogenase 3		PRO_0000182747
Sites
	Active site	102	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9S7A0-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: B972A0ABBDA87D59	FASTA	411	44,528
	10 20 30 40 50 60 MNALAATNRN FKLASRLLGL DSKLEKSLLI PFREIKVECT IPKDDGTLAS FVGFRVQHDN 70 80 90 100 110 120 ARGPMKGGIR YHPEVEPDEV NALAQLMTWK TAVAKIPYGG AKGGIGCDPS ELSLSELERL 130 140 150 160 170 180 TRVFTQKIHD LIGIHTDVPA PDMGTGPQTM AWILDEYSKF HGHSPAVVTG KPIDLGGSLG 190 200 210 220 230 240 RDAATGRGVL FATEALLNEH GKTISGQRFA IQGFGNVGSW AAKLISDKGG KIVAVSDVTG 250 260 270 280 290 300 AIKNNNGIDI LSLLEHAEEN RGIKGFDGAD SIDPDSILVE DCDILVPAAL GGVINRENAN 310 320 330 340 350 360 EIKAKFIIEG ANHPTDPEAD EILKKKGVMI LPDIYANSGG VTVSYFEWVQ NIQGFMWDEE 370 380 390 400 410 KVNRELKTYM TRGFKDLKEM CQTHSCDLRM GAFTLGINRV AQATTIRGWG S

References

[1]

"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. Nature 408:820-822(2000) [PubMed: 11130713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC011698 Genomic DNA. Translation: AAF05851.1. AC009540 Genomic DNA. Translation: AAF00627.1.
IPI	IPI00538151.
RefSeq	NP_187041.1.
UniGene	At.27423 At.53173 Rra.10053
3D structure databases
SMR	Q9S7A0. Positions 6-408.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9S7A0.
Proteomic databases
PRIDE	Q9S7A0.
ProMEX	Q9S7A0.
Genome annotation databases
GeneID	821072.
GenomeReviews	Gene locus AT3G03910 in contig BA000014_GR.
KEGG	ath:AT3G03910.
NMPDR	fig|3702.1.peg.12351.
Organism-specific databases
GeneFarm	2267. 178.
TAIR	At3g03910.
Phylogenomic databases
eggNOG	KOG2250.
HOGENOM	HBG590661.
InParanoid	Q9S7A0.
OMA	NAFLENT.
PhylomeDB	Q9S7A0.
Enzyme and pathway databases
BRENDA	1.4.1.3. 302.
Gene expression databases
ArrayExpress	Q9S7A0.
Genevestigator	Q9S7A0.
GermOnline	AT3G03910. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR006095. Glu/Leu/Phe/Val_DH. IPR006096. Glu/Leu/Phe/Val_DH_C. IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom. IPR014362. Glu_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11606:SF2. GLFV_DH. 1 hit.
Pfam	PF00208. ELFV_dehydrog. 1 hit. PF02812. ELFV_dehydrog_N. 1 hit. [Graphical view]
PIRSF	PIRSF000185. Glu_DH. 1 hit.
PRINTS	PR00082. GLFDHDRGNASE.
SMART	SM00839. ELFV_dehydrog. 1 hit. [Graphical view]
PROSITE	PS00074. GLFV_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHE3_ARATH

Accession

Primary (citable) accession number: Q9S7A0

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 2000
Last modified:	February 9, 2010
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)