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Protein

Two-pore potassium channel 5

Gene

TPK5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probable voltage-independent potassium-selective tonoplast ion channel.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi352 – 3631Sequence analysisAdd BLAST12
Calcium bindingi391 – 4022Sequence analysisAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIon channel, Potassium channel
Biological processIon transport, Potassium transport, Transport
LigandCalcium, Metal-binding, Potassium

Protein family/group databases

TCDBi1.A.1.7.5 the voltage-gated ion channel (vic) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
Two-pore potassium channel 5
Short name:
AtTPK5
Alternative name(s):
Calcium-activated outward-rectifying potassium channel 5, chloroplastic
Short name:
AtKCO5
Gene namesi
Name:TPK5
Synonyms:KCO5
Ordered Locus Names:At4g01840
ORF Names:T7B11.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G01840
TAIRilocus:2141375 AT4G01840

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini22 – 115StromalSequence analysisAdd BLAST94
Transmembranei116 – 136HelicalSequence analysisAdd BLAST21
Intramembranei152 – 171Pore-forming; Name=Pore-forming 1Sequence analysisAdd BLAST20
Transmembranei178 – 198HelicalSequence analysisAdd BLAST21
Topological domaini199 – 248StromalSequence analysisAdd BLAST50
Transmembranei249 – 269HelicalSequence analysisAdd BLAST21
Intramembranei276 – 295Pore-forming; Name=Pore-forming 2Sequence analysisAdd BLAST20
Transmembranei302 – 322HelicalSequence analysisAdd BLAST21
Topological domaini323 – 408StromalSequence analysisAdd BLAST86

Keywords - Cellular componenti

Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000358731 – 408Two-pore potassium channel 5Add BLAST408

Proteomic databases

PaxDbiQ9S6Z8

PTM databases

iPTMnetiQ9S6Z8

Expressioni

Tissue specificityi

Expressed in hydathodes and the vascular tissues of roots, stems, leaves and flowers.1 Publication

Gene expression databases

ExpressionAtlasiQ9S6Z8 baseline and differential
GenevisibleiQ9S6Z8 AT

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi13382, 3 interactors
STRINGi3702.AT4G01840.1

Structurei

3D structure databases

ProteinModelPortaliQ9S6Z8
SMRiQ9S6Z8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini339 – 374EF-hand 1Add BLAST36
Domaini378 – 408EF-hand 2Add BLAST31

Domaini

Each of the two pore-forming region (also called P-domain or P-loop) is enclosed by two transmembrane segments (2P/4TM) and contains the GYGD signature motif which seems to be involved in potassium selectivity.

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1418 Eukaryota
COG1226 LUCA
HOGENOMiHOG000238193
InParanoidiQ9S6Z8
KOiK05389
OMAiHRRITNW
OrthoDBiEOG09360JKS
PhylomeDBiQ9S6Z8

Family and domain databases

InterProiView protein in InterPro
IPR003280 2pore_dom_K_chnl
IPR013099 K_chnl_dom
PfamiView protein in Pfam
PF07885 Ion_trans_2, 2 hits
PRINTSiPR01333 2POREKCHANEL

Sequencei

Sequence statusi: Complete.

Q9S6Z8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPLISPQPR FRLQPIPENP SSSSSSASIT IPRSISNTSF FHEISQERLL
60 70 80 90 100
LHHQDLEQSV QDDKEDQDSD SDETNRFLSQ TRPLHRSRTA PAMVIIKDLR
110 120 130 140 150
TKPPETKKPS PVSKSIIRQA IFLLIVYLTL GVSIYSFNRD HYSGIETHPV
160 170 180 190 200
VDALYFCIVT MCTIGYGDIA PLTPWTKIFA VVFVLFGFGF LDILLSGVVN
210 220 230 240 250
YVLDLQESMI LTGIQTRQHH QHHHHHRFSA KDYIIDFEKG RMRIRMKVCL
260 270 280 290 300
ALCVVVLCIG VGALVLHFVE ELGFVDSVYL SVMSVTTVGY GDRAFKTLQG
310 320 330 340 350
RLFAAVWLLV STLAVARAFL YLAEARIDRR HRKAVKLALN REITVDDLLK
360 370 380 390 400
ADTYQHGFIS KSEYIVLKLK EMGKITQKDI DQVVIQFEKL DPNQIGKITL

PDLLGDPL
Length:408
Mass (Da):46,259
Last modified:May 1, 2000 - v1
Checksum:iFCF22402122F8F2A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ243456 mRNA Translation: CAB62162.1
AC007138 Genomic DNA Translation: AAD22646.1
AL161493 Genomic DNA Translation: CAB80677.1
CP002687 Genomic DNA Translation: AEE82083.1
AY084857 mRNA Translation: AAM61421.1
PIRiF85023
RefSeqiNP_192093.1, NM_116414.4
UniGeneiAt.585
At.66593

Genome annotation databases

EnsemblPlantsiAT4G01840.1; AT4G01840.1; AT4G01840
GeneIDi828091
GrameneiAT4G01840.1; AT4G01840.1; AT4G01840
KEGGiath:AT4G01840

Similar proteinsi

Entry informationi

Entry nameiKCO5_ARATH
AccessioniPrimary (citable) accession number: Q9S6Z8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: May 1, 2000
Last modified: April 25, 2018
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health