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Protein

Two-pore potassium channel 5

Gene

TPK5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Probable voltage-independent potassium-selective tonoplast ion channel.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi352 – 3631Sequence analysisAdd BLAST12
Calcium bindingi391 – 4022Sequence analysisAdd BLAST12

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Potassium channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Calcium, Metal-binding, Potassium

Protein family/group databases

TCDBi1.A.1.7.5. the voltage-gated ion channel (vic) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Two-pore potassium channel 5
Short name:
AtTPK5
Alternative name(s):
Calcium-activated outward-rectifying potassium channel 5, chloroplastic
Short name:
AtKCO5
Gene namesi
Name:TPK5
Synonyms:KCO5
Ordered Locus Names:At4g01840
ORF Names:T7B11.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G01840.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini22 – 115StromalSequence analysisAdd BLAST94
Transmembranei116 – 136HelicalSequence analysisAdd BLAST21
Intramembranei152 – 171Pore-forming; Name=Pore-forming 1Sequence analysisAdd BLAST20
Transmembranei178 – 198HelicalSequence analysisAdd BLAST21
Topological domaini199 – 248StromalSequence analysisAdd BLAST50
Transmembranei249 – 269HelicalSequence analysisAdd BLAST21
Intramembranei276 – 295Pore-forming; Name=Pore-forming 2Sequence analysisAdd BLAST20
Transmembranei302 – 322HelicalSequence analysisAdd BLAST21
Topological domaini323 – 408StromalSequence analysisAdd BLAST86

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plant-type vacuole membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000358731 – 408Two-pore potassium channel 5Add BLAST408

Proteomic databases

PaxDbiQ9S6Z8.

PTM databases

iPTMnetiQ9S6Z8.

Expressioni

Tissue specificityi

Expressed in hydathodes and the vascular tissues of roots, stems, leaves and flowers.1 Publication

Gene expression databases

GenevisibleiQ9S6Z8. AT.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi13382. 3 interactors.
STRINGi3702.AT4G01840.1.

Structurei

3D structure databases

ProteinModelPortaliQ9S6Z8.
SMRiQ9S6Z8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini339 – 374EF-hand 1Add BLAST36
Domaini378 – 408EF-hand 2Add BLAST31

Domaini

Each of the two pore-forming region (also called P-domain or P-loop) is enclosed by two transmembrane segments (2P/4TM) and contains the GYGD signature motif which seems to be involved in potassium selectivity.

Sequence similaritiesi

Contains 2 EF-hand domains.Curated

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1418. Eukaryota.
COG1226. LUCA.
HOGENOMiHOG000238193.
InParanoidiQ9S6Z8.
KOiK05389.
OMAiHRRITNW.
OrthoDBiEOG09360JKS.
PhylomeDBiQ9S6Z8.

Family and domain databases

InterProiIPR003280. 2pore_dom_K_chnl.
IPR013099. K_chnl_dom.
[Graphical view]
PfamiPF07885. Ion_trans_2. 2 hits.
[Graphical view]
PRINTSiPR01333. 2POREKCHANEL.

Sequencei

Sequence statusi: Complete.

Q9S6Z8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPLISPQPR FRLQPIPENP SSSSSSASIT IPRSISNTSF FHEISQERLL
60 70 80 90 100
LHHQDLEQSV QDDKEDQDSD SDETNRFLSQ TRPLHRSRTA PAMVIIKDLR
110 120 130 140 150
TKPPETKKPS PVSKSIIRQA IFLLIVYLTL GVSIYSFNRD HYSGIETHPV
160 170 180 190 200
VDALYFCIVT MCTIGYGDIA PLTPWTKIFA VVFVLFGFGF LDILLSGVVN
210 220 230 240 250
YVLDLQESMI LTGIQTRQHH QHHHHHRFSA KDYIIDFEKG RMRIRMKVCL
260 270 280 290 300
ALCVVVLCIG VGALVLHFVE ELGFVDSVYL SVMSVTTVGY GDRAFKTLQG
310 320 330 340 350
RLFAAVWLLV STLAVARAFL YLAEARIDRR HRKAVKLALN REITVDDLLK
360 370 380 390 400
ADTYQHGFIS KSEYIVLKLK EMGKITQKDI DQVVIQFEKL DPNQIGKITL

PDLLGDPL
Length:408
Mass (Da):46,259
Last modified:May 1, 2000 - v1
Checksum:iFCF22402122F8F2A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ243456 mRNA. Translation: CAB62162.1.
AC007138 Genomic DNA. Translation: AAD22646.1.
AL161493 Genomic DNA. Translation: CAB80677.1.
CP002687 Genomic DNA. Translation: AEE82083.1.
AY084857 mRNA. Translation: AAM61421.1.
PIRiF85023.
RefSeqiNP_192093.1. NM_116414.4.
UniGeneiAt.585.
At.66593.

Genome annotation databases

EnsemblPlantsiAT4G01840.1; AT4G01840.1; AT4G01840.
GeneIDi828091.
GrameneiAT4G01840.1; AT4G01840.1; AT4G01840.
KEGGiath:AT4G01840.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ243456 mRNA. Translation: CAB62162.1.
AC007138 Genomic DNA. Translation: AAD22646.1.
AL161493 Genomic DNA. Translation: CAB80677.1.
CP002687 Genomic DNA. Translation: AEE82083.1.
AY084857 mRNA. Translation: AAM61421.1.
PIRiF85023.
RefSeqiNP_192093.1. NM_116414.4.
UniGeneiAt.585.
At.66593.

3D structure databases

ProteinModelPortaliQ9S6Z8.
SMRiQ9S6Z8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi13382. 3 interactors.
STRINGi3702.AT4G01840.1.

Protein family/group databases

TCDBi1.A.1.7.5. the voltage-gated ion channel (vic) superfamily.

PTM databases

iPTMnetiQ9S6Z8.

Proteomic databases

PaxDbiQ9S6Z8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G01840.1; AT4G01840.1; AT4G01840.
GeneIDi828091.
GrameneiAT4G01840.1; AT4G01840.1; AT4G01840.
KEGGiath:AT4G01840.

Organism-specific databases

TAIRiAT4G01840.

Phylogenomic databases

eggNOGiKOG1418. Eukaryota.
COG1226. LUCA.
HOGENOMiHOG000238193.
InParanoidiQ9S6Z8.
KOiK05389.
OMAiHRRITNW.
OrthoDBiEOG09360JKS.
PhylomeDBiQ9S6Z8.

Miscellaneous databases

PROiQ9S6Z8.

Gene expression databases

GenevisibleiQ9S6Z8. AT.

Family and domain databases

InterProiIPR003280. 2pore_dom_K_chnl.
IPR013099. K_chnl_dom.
[Graphical view]
PfamiPF07885. Ion_trans_2. 2 hits.
[Graphical view]
PRINTSiPR01333. 2POREKCHANEL.
ProtoNetiSearch...

Entry informationi

Entry nameiKCO5_ARATH
AccessioniPrimary (citable) accession number: Q9S6Z8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: May 1, 2000
Last modified: November 30, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.