Q9S646 (PPK_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Polyphosphate kinase
EC=2.7.4.1

Alternative name(s):
ATP-polyphosphate phosphotransferase
Polyphosphoric acid kinase

Gene names

Name:	ppk
Ordered Locus Names:	PA5242

Organism

Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) [Reference proteome] [HAMAP]

Taxonomic identifier

208964 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas aeruginosa group › Pseudomonas aeruginosa

Protein attributes

Sequence length	690 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible transfer of the terminal phosphate of ATP to form a long-chain polyphosphate (polyP). HAMAP-Rule MF_00347
Catalytic activity	ATP + (phosphate)(n) = ADP + (phosphate)(n+1). HAMAP-Rule MF_00347
Post-translational modification	An intermediate of this reaction is the autophosphorylated ppk in which a phosphate is covalently linked to histidine residues through a N-P bond By similarity. HAMAP-Rule MF_00347
Sequence similarities	Belongs to the polyphosphate kinase family.
Sequence caution	The sequence AAG08627.1 differs from that shown. Reason: Erroneous initiation. The sequence BAA22565.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	polyphosphate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	polyphosphate kinase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW polyphosphate kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 690	690	Polyphosphate kinase HAMAP-Rule MF_00347		PRO_0000128652
Sites
	Active site	435	1	Phosphohistidine intermediate By similarity
	Active site	454	1	Phosphohistidine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9S646 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 7F215EDD7F35742E	FASTA	690	78,293
	10 20 30 40 50 60 MDDSSLYIHR ELSQLQFNIR VLEQALDESY PLLERLKFLL IFSSNLDEFF EIRIAGLKKQ 70 80 90 100 110 120 ITFAREQAGA DGLLPHQALA RISELVHEQV SRQYRILNET LLPELAKHQI RFIRRRHWTL 130 140 150 160 170 180 KIKTWVRRFF RDEIAPIITP IGLDPTHPFP LLVNKSLNFI VELEGMDAFG RDSGLAIIPA 190 200 210 220 230 240 PRLLPRIIRL PEDVGGEGDN YVFLSSMIHA HADDLFPGMK VKGCYQFRLT RNADLSVDTE 250 260 270 280 290 300 DVEDLARALR GELFSRRYGD AVRLEVVDTC PQNLTNYLLK QFGLSESELY KVSGPVNLTR 310 320 330 340 350 360 LFSVTGLESH PELQYPPFTP AIPRLLQKKE NLFNVLSKLD VLLMHPFESF TPVIDLLRQA 370 380 390 400 410 420 AKDPNVLAIK QTLYRSGANS EIVDALVEAA RNGKEVTAVI ELRARFDEES NLQLASRLQQ 430 440 450 460 470 480 AGAVVIYGVV GFKTHAKMML ILRREDGELR RYAHLGTGNY HAGNARLYTD YSLLTADVAL 490 500 510 520 530 540 CEDLHKLFNQ LIGMGKTLRM KKLLHAPFTL KKNLLEMINR EAAQAALGQP AHIMAKVNSL 550 560 570 580 590 600 TDPKVIRALY KASQAGVRID LVVRGMCCLR PGIPGVSHNI HVRSIIGRFL EHSRIYYFLN 610 620 630 640 650 660 GGDEKLYLSS ADWMERNLDM RVETCFPVEG KKLVQRVKKE LETYLTDNTQ AWVLQADGSY 670 680 690 QRLSPTGNQN PRNTQATLLE KLAAPVLTAR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The polyphosphate kinase gene of Pseudomonas aeruginosa." Ishige K., Kameda A., Noguchi T., Shiba T. DNA Res. 5:157-162(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[2]	"Cloning and characterization of polyphosphate kinase and exopolyphosphatase genes from Pseudomonas aeruginosa 8830." Zago A., Chugani S., Chakrabarty A.M. Appl. Environ. Microbiol. 65:2065-2071(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 8830.
[3]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB007598 Genomic DNA. Translation: BAA22565.1. Different initiation. AF087931 Genomic DNA. Translation: AAD29112.1. AE004091 Genomic DNA. Translation: AAG08627.1. Different initiation.
PIR	JE0187.
RefSeq	NP_253929.1. NC_002516.2.
3D structure databases
ProteinModelPortal	Q9S646.
SMR	Q9S646. Positions 7-651.
ModBase	Search...
Protein-protein interaction databases
STRING	208964.PA5242.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	879700.
KEGG	pae:PA5242.
PATRIC	19845335. VBIPseAer58763_5494.
Organism-specific databases
PseudoCAP	PA5242.
Phylogenomic databases
eggNOG	COG0855.
HOGENOM	HOG000248948.
KO	K00937.
OMA	LSQLQFN.
ProtClustDB	PRK05443.
Enzyme and pathway databases
BRENDA	2.7.4.1. 5087.
Family and domain databases
Gene3D	3.30.1840.10. 1 hit.
HAMAP	MF_00347. Polyphosphate_kinase.
InterPro	IPR003414. PP_kinase. IPR024953. PP_kinase_middle. IPR025200. PPK_C_dom. IPR025198. PPK_N_dom. [Graphical view]
Pfam	PF02503. PP_kinase. 1 hit. PF13090. PP_kinase_C. 1 hit. PF13089. PP_kinase_N. 1 hit. [Graphical view]
PIRSF	PIRSF015589. PP_kinase. 1 hit.
SUPFAM	SSF140356. SSF140356. 1 hit.
TIGRFAMs	TIGR03705. poly_P_kin. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PPK_PSEAE

Accession

Primary (citable) accession number: Q9S646
Secondary accession number(s): O24816

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	May 1, 2000
Last modified:	May 1, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program