Q9S5Z2 (CLPE_LACLM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent Clp protease ATP-binding subunit ClpE

Gene names

Name:	clpE
Ordered Locus Names:	llmg_0528

Organism

Lactococcus lactis subsp. cremoris (strain MG1363) [Complete proteome] [HAMAP]

Taxonomic identifier

416870 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Lactococcus › Lactococcus lactis › Lactococcus lactis subsp. cremoris

Protein attributes

Sequence length	748 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Along with ClpP could be necessary for degrading proteins generated by certain types of stress.
Induction	By heat shock.
Miscellaneous	Disruption of ClpE reduces tolerance for puromycin.
Sequence similarities	Belongs to the clpA/clpB family. ClpE subfamily. Contains 1 UVR domain.

Ontologies

Keywords
Biological process	Stress response
Domain	Repeat Zinc-finger
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Chaperone
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 748	748	ATP-dependent Clp protease ATP-binding subunit ClpE		PRO_0000191226
Regions
	Domain	359 – 394	36	UVR
	Zinc finger	3 – 32	30	C4-type Potential
	Nucleotide binding	152 – 159	8	ATP Potential
	Nucleotide binding	481 – 488	8	ATP Potential
	Region	107 – 354	248	I
	Region	407 – 598	192	II
Experimental info
	Sequence conflict	476	1	S → F in AAD01782. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9S5Z2 [UniParc]. Last modified May 1, 2007. Version 2. Checksum: EF5F14E3211AA94A	FASTA	748	83,206
	10 20 30 40 50 60 MLCQNCNINE ATIHLYTSVN GQKKQIDLCQ NCYQIMKSGG QEALFGAGNA SNGNSDEPFN 70 80 90 100 110 120 PFNDIFSALQ GQDFNGAASN QTPPTQTGGR GPRGPQNPRA KQPKGMLEEF GINITESARR 130 140 150 160 170 180 GEIDPVIGRD EEIKRVIEIL NRRTKNNPVL IGEPGVGKTA VVEGLAQKIV DGDVPQKLQN 190 200 210 220 230 240 KEVIRLDVVS LVQGTGIRGQ FEERMQKLMD EIRKRNDVIM FIDEIHEIVG AGSAGDGNMD 250 260 270 280 290 300 AGNILKPALA RGELQLVGAT TLNEYRIIEK DAALERRMQP VKVDEPSVDE TITILRGIQA 310 320 330 340 350 360 RYEDYHHVKY TDEAIEAAAH LSNRYIQDRF LPDKAIDLLD ESGSKKNLTL KFVDPEDINR 370 380 390 400 410 420 RIADAESKKN EATKAEDFEK AAHFRDQISK LRELQKQEVT DEDMPVITEK DIEQIVEQKT 430 440 450 460 470 480 QIPVGDLKEK EQTQLINLAD DLKAHVIGQD EAVDKISKAI RRSRVGLGKP NRPIGSFLFV 490 500 510 520 530 540 GPTGVGKTEL AKQLAKELFG SSESMIRFDM SEYMEKHSVA KLIGAPPGYV GYEEAGQLTE 550 560 570 580 590 600 RVRRNPYSLI LLDEIEKAHP DVMHMFLQIL EDGRLTDAQG RTVSFKDSLI IMTSNAGTGK 610 620 630 640 650 660 VEASVGFGAA REGRTKSVLG QLGDFFSPEF MNRFDGIIEF SALSKENLLK IVDLMLDEVN 670 680 690 700 710 720 EQIGRNDIHL SVTQAAKEKL VDLGYNPAMG ARPLRRIIQE NIEDSIADFY IEHPEYKQLV 730 740 ADLIDDKIVI SNQTQETAET TDEEVPAE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Disruption and analysis of the clpB, clpC, and clpE genes in Lactococcus lactis: clpE, a new Clp family in Gram-positive bacteria." Ingmer H., Vogensen F.K., Hammer K., Kilstrup M. J. Bacteriol. 181:2075-2083(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
[2]	"The complete genome sequence of the lactic acid bacterial paradigm Lactococcus lactis subsp. cremoris MG1363." Wegmann U., O'Connell-Motherway M., Zomer A., Buist G., Shearman C., Canchaya C., Ventura M., Goesmann A., Gasson M.J., Kuipers O.P., van Sinderen D., Kok J. J. Bacteriol. 189:3256-3270(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MG1363.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF023421 Genomic DNA. Translation: AAD01782.1. AM406671 Genomic DNA. Translation: CAL97132.1.
RefSeq	YP_001031878.1. NC_009004.1.
3D structure databases
ProteinModelPortal	Q9S5Z2.
SMR	Q9S5Z2. Positions 107-296.
ModBase	Search...
Protein-protein interaction databases
STRING	416870.llmg_0528.
Proteomic databases
PRIDE	Q9S5Z2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAL97132; CAL97132; llmg_0528.
GeneID	4797303.
KEGG	llm:llmg_0528.
PATRIC	22282143. VBILacLac4574_0539.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0542.
HOGENOM	HOG000218211.
KO	K03697.
OMA	RTIQDHI.
ProtClustDB	CLSK876751.
Family and domain databases
Gene3D	4.10.860.10. 1 hit.
InterPro	IPR003593. AAA+_ATPase. IPR013093. ATPase_AAA-2. IPR003959. ATPase_AAA_core. IPR018368. Chaperonin_ClpA/B_CS. IPR001270. Chaprnin_ClpA/B. IPR019489. Clp_ATPase_C. IPR001943. UVR_dom. [Graphical view]
Pfam	PF00004. AAA. 1 hit. PF07724. AAA_2. 1 hit. PF10431. ClpB_D2-small. 1 hit. PF02151. UVR. 1 hit. [Graphical view]
PRINTS	PR00300. CLPPROTEASEA.
SMART	SM00382. AAA. 2 hits. SM01086. ClpB_D2-small. 1 hit. [Graphical view]
SUPFAM	SSF46600. UvrB_C. 1 hit.
PROSITE	PS00870. CLPAB_1. 1 hit. PS00871. CLPAB_2. 1 hit. PS50151. UVR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CLPE_LACLM

Accession

Primary (citable) accession number: Q9S5Z2
Secondary accession number(s): A2RIN7

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	May 1, 2007
Last modified:	May 1, 2013
This is version 75 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program