Reviewed, UniProtKB/Swiss-Prot Q9S5X0 (HPPA_THEMA)

Names and origin

Protein names

Recommended name:
    Pyrophosphate-energized proton pump
    EC=3.6.1.1
Alternative name(s):
    Pyrophosphate-energized inorganic pyrophosphatase
      Short name=H(+)-PPase
    Membrane-bound proton-translocating pyrophosphatase

Gene names

Name:	hppA
Ordered Locus Names:	TM_0174

Organism

Thermotoga maritima [Complete proteome] [HAMAP]

Taxonomic identifier

2336 [NCBI]

Taxonomic lineage

Bacteria › Thermotogae › Thermotogales › Thermotogaceae › Thermotoga

Protein attributes

Sequence length	726 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Generates a proton motive force; it probably catalyzes a fully reversible reaction, thus being able to synthesize pyrophosphate when the proton motive force is sufficient. HAMAP MF_01129
Catalytic activity	Diphosphate + H2O = 2 phosphate. HAMAP MF_01129
Cofactor	Magnesium. HAMAP MF_01129 Potassium. HAMAP MF_01129
Subunit structure	Homodimer Potential.
Subcellular location	Cell membrane; Multi-pass membrane protein Probable.
Sequence similarities	Belongs to the H(+)-translocating pyrophosphatase (TC 3.A.10) family. Type 1 subfamily. [View classification]

Ontologies

Keywords
Biological process	Hydrogen ion transport Ion transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Ligand	Magnesium Potassium
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proton transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	hydrogen-translocating pyrophosphatase activity Inferred from electronic annotation. Source: InterPro inorganic diphosphatase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP potassium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 726	726	Pyrophosphate-energized proton pump HAMAP MF_01129		PRO_0000217005
Regions
	Transmembrane	4 – 26	23	Potential
	Transmembrane	60 – 82	23	Potential
	Transmembrane	125 – 147	23	Potential
	Transmembrane	167 – 189	23	Potential
	Transmembrane	245 – 267	23	Potential
	Transmembrane	288 – 310	23	Potential
	Transmembrane	325 – 344	20	Potential
	Transmembrane	357 – 379	23	Potential
	Transmembrane	424 – 446	23	Potential
	Transmembrane	500 – 522	23	Potential
	Transmembrane	542 – 564	23	Potential
	Transmembrane	612 – 631	20	Potential
	Transmembrane	636 – 658	23	Potential
Sites
	Site	495	1	Determinant of potassium dependence By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9S5X0-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 492243F8C320BF93	FASTA	726	77,046
	10 20 30 40 50 60 MYVAALFFLI PLVALGFAAA NFAAVVRKPE GTERMKEISS YIRSGADSFL AHETKAIFKV 70 80 90 100 110 120 AIVIAILLMI FTTWQTGVAF LLGAVMSASA GIVGMKMATR ANVRVAEAAR TTKKIGPALK 130 140 150 160 170 180 VAYQGGSVMG LSVGGFALLG LVLVYLIFGK WMGQVDNLNI YTNWLGINFV PFAMTVSGYA 190 200 210 220 230 240 LGCSIIAMFD RVGGGVYTKA ADMAADLVGK TELNLPEDDP RNPATIADNV GDNVGDVAGL 250 260 270 280 290 300 GADLLESFVG AIVSSIILAS YMFPIYVQKI GENLVHQVPK ETIQALISYP IFFALVGLGC 310 320 330 340 350 360 SMLGILYVIV KKPSDNPQRE LNISLWTSAL LTVVLTAFLT YFYLKDLQGL DVVGFRFGAI 370 380 390 400 410 420 SPWFSAIIGI FSGILIGFWA EYYTSYRYKP TQFLSKSSIE GTGMVISNGL SLGMKSVFPP 430 440 450 460 470 480 TLTLVLGILF ADYFAGLYGV AIAALGMLSF VATSVSVDSY GPIADNAGGI SEMCELDPEV 490 500 510 520 530 540 RKITDHLDAV GNTTAAIGKG FAIGSAIFAA LSLFASYMFS QISPSDIGKP PSLVLLLNML 550 560 570 580 590 600 DARVIAGALL GAAITYYFSG YLISAVTKAA MKMVDEIRRQ AREIPGLLEG KAKPDYNRCI 610 620 630 640 650 660 EITSDNALKQ MGYPAFIAIL TPLVTGFLLG AEFVGGVLIG TVLSGAMLAI LTANSGGAWD 670 680 690 700 710 720 NAKKYLEAGN LEGYGKGSEP HKALVIGDTV GDPLKDTVGP SLDILIKIMS VVSVIAVSIF KHVHLF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Evidence for lateral gene transfer between Archaea and Bacteria from genome sequence of Thermotoga maritima." Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.A., Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M., Cotton M.D., Pratt M.S. , Phillips C.A., Richardson D.L., Heidelberg J.F., Sutton G.G., Fleischmann R.D., Eisen J.A., White O., Salzberg S.L., Smith H.O., Venter J.C., Fraser C.M. Nature 399:323-329(1999) [PubMed: 10360571] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
[2]	"Reverse gyrase from the hyperthermophilic bacterium Thermotoga maritima: properties and gene structure." Bouthier de la Tour C., Portemer C., Kaltoum H., Duguet M. J. Bacteriol. 180:274-281(1998) [PubMed: 9440516] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-276. Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
[3]	"A thermostable K(+)-stimulated vacuolar-type pyrophosphatase from the hyperthermophilic bacterium Thermotoga maritima." Perez-Castineira J.R., Lopez-Marques R.L., Losada M., Serrano A. FEBS Lett. 496:6-11(2001) [PubMed: 11343697] [Abstract] Cited for: CHARACTERIZATION. Strain: ATCC 43589 / MSB8 / DSM 3109 / JCM 10099.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000512 Genomic DNA. Translation: AAD35267.1. AF013268 Genomic DNA. Translation: AAC01564.2.
PIR	D72409.
RefSeq	NP_227989.1.
3D structure databases
ModBase	Search...
Protein family/group databases
TCDB	3.A.10.1.4. H+-translocating pyrophosphatase (H+-PPase) family.
Genome annotation databases
GeneID	897014.
GenomeReviews	Gene locus TM_0174 in contig AE000512_GR.
KEGG	tma:TM0174.
NMPDR	fig|243274.1.peg.173.
TIGR	TM_0174.
Phylogenomic databases
HOGENOM	Q9S5X0.
OMA	KMATRAN.
Enzyme and pathway databases
BioCyc	TMAR243274:TM_0174-MON.
BRENDA	3.6.1.1. 16699.
Family and domain databases
HAMAP	MF_01129. [Tree]
InterPro	IPR004131. H_PPase. [Graphical view]
Pfam	PF03030. H_PPase. 1 hit. [Graphical view]
PIRSF	PIRSF001265. H+-PPase. 1 hit.
TIGRFAMs	TIGR01104. V_PPase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HPPA_THEMA

Accession

Primary (citable) accession number: Q9S5X0
Secondary accession number(s): O51935

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2003
Last sequence update:	May 1, 2000
Last modified:	November 3, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)