Q9S5V6 (ALR_BACPS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine racemase
EC=5.1.1.1

Gene names

Name:

alr

Organism

Bacillus psychrosaccharolyticus

Taxonomic identifier

1407 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	383 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Provides the D-alanine required for cell wall biosynthesis. HAMAP MF_01201
Catalytic activity	L-alanine = D-alanine. HAMAP MF_01201
Cofactor	Pyridoxal phosphate.
Pathway	Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine from L-alanine: step 1/1. HAMAP MF_01201 Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_01201
Subunit structure	Homodimer.
Sequence similarities	Belongs to the alanine racemase family.
Biophysicochemical properties	pH dependence: Optimum pH is 8-10. HAMAP MF_01201 Temperature dependence: Highly active at low temperatures, even at 0 degree Celsius. Thermolabile.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	alanine metabolic process Inferred from electronic annotation. Source: InterPro cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alanine racemase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 383	383	Alanine racemase HAMAP MF_01201		PRO_0000114498
Sites
	Active site	41	1	Proton acceptor; specific for D-alanine By similarity
	Active site	266	1	Proton acceptor; specific for L-alanine By similarity
Amino acid modifications
	Modified residue	41	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9S5V6 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: E6DE9421EEFC5BF7	FASTA	383	42,519
	10 20 30 40 50 60 MTLQNFYRDT WAEINLDAIF ENAANMKKHL PPEITLFAVV KANAYGHGDV EVAETAIQAG 70 80 90 100 110 120 AGYLAVAFLD EALALRKKGI TAPILVLGAS RPEDAQIAAR ESITLTVFQA GWLEAAQSFL 130 140 150 160 170 180 EGTVLTIHLK CDSGMGRIGI RKQEEMNEIE RFLQKTKCFV LEGIFTHFAT ADQLDTEYFS 190 200 210 220 230 240 KQLARFEEML TWLKEKPKYV HAANSAALLR FPNAVFNSVR MGISLYGLSP SMEMKEVLPF 250 260 270 280 290 300 ALRPAFSLKT KLVHVKNISK GQSVSYGATY TAEEDTWIGT LPIGYADGWI RMLQGQEVLL 310 320 330 340 350 360 EGGRSPLVGR ICMDQCMVKL SREFPVGTEV TLIGKNGTEC ITVDDIAEKL NTINYEVTCM 370 380 ISSRVPRMYL KDGRITGVVN QLI

References

[1]	"Characterization of psychrophilic alanine racemase from Bacillus psychrosaccharolyticus." Okubo Y., Yokoigawa K., Esaki N., Soda K., Kawai H. Biochem. Biophys. Res. Commun. 256:333-340(1999) [PubMed: 10080917] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-50.
[2]	"Alanine racemase gene fragments as probes for detecting Bacillus stearothermophilus and Bacillus psychrosaccharolyticus in foods." Okubo Y., Yokoigawa K., Kawai H. J. Ferment. Bioeng. 85:559-563(1998) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 48-166.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB021683 Genomic DNA. Translation: BAA76373.1. AB011668 Genomic DNA. Translation: BAA31255.1.
3D structure databases
ProteinModelPortal	Q9S5V6.
SMR	Q9S5V6. Positions 5-382.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BRENDA	5.1.1.1. 7181.
Family and domain databases
HAMAP	MF_01201. Ala_racemase. [Tree]
InterPro	IPR000821. Ala_racemase. IPR009006. Ala_racemase/Decarboxylase_C. IPR011079. Ala_racemase_C. IPR001608. Ala_racemase_N. IPR020622. Ala_racemase_pyridoxalP-BS. [Graphical view]
Gene3D	G3DSA:2.40.37.10. Ala_racemase/Decarboxylase_C. 1 hit.
Pfam	PF00842. Ala_racemase_C. 1 hit. PF01168. Ala_racemase_N. 1 hit. [Graphical view]
PRINTS	PR00992. ALARACEMASE.
SMART	SM01005. Ala_racemase_C. 1 hit. [Graphical view]
SUPFAM	SSF50621. Racem_decarbox_C. 1 hit.
TIGRFAMs	TIGR00492. Alr. 1 hit.
PROSITE	PS00395. ALANINE_RACEMASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALR_BACPS

Accession

Primary (citable) accession number: Q9S5V6
Secondary accession number(s): O82941

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 5, 2002
Last sequence update:	May 1, 2000
Last modified:	January 25, 2012
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program