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Q9S5G4

- HIS4_ECO57

UniProt

Q9S5G4 - HIS4_ECO57

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Protein

1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase

Gene

hisA

Organism
Escherichia coli O157:H7
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

1-(5-phospho-beta-D-ribosyl)-5-((5-phospho-beta-D-ribosylamino)methylideneamino)imidazole-4-carboxamide = 5-((5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino)-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei7 – 71Proton acceptorBy similarity
Active sitei129 – 1291Proton donorBy similarity

GO - Molecular functioni

  1. 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. histidine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Amino-acid biosynthesis, Histidine biosynthesis

Enzyme and pathway databases

BioCyciECOL386585:GJFA-2789-MONOMER.
UniPathwayiUPA00031; UER00009.

Names & Taxonomyi

Protein namesi
Recommended name:
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (EC:5.3.1.16)
Alternative name(s):
Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase
Gene namesi
Name:hisA
Ordered Locus Names:Z3186, ECs2825
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000558: Chromosome, UP000002519: Chromosome

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2452451-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerasePRO_0000142006Add
BLAST

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi155864.Z3186.

Structurei

3D structure databases

ProteinModelPortaliQ9S5G4.
SMRiQ9S5G4. Positions 2-242.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the HisA/HisF family.Curated

Phylogenomic databases

eggNOGiCOG0106.
HOGENOMiHOG000224614.
KOiK01814.
OMAiTERIMYK.
OrthoDBiEOG6H1Q3W.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01014. HisA.
InterProiIPR013785. Aldolase_TIM.
IPR006062. His_biosynth.
IPR006063. HisA.
IPR023016. Isoase_HisA.
IPR011060. RibuloseP-bd_barrel.
[Graphical view]
PfamiPF00977. His_biosynth. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 1 hit.
TIGRFAMsiTIGR00007. TIGR00007. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9S5G4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MIIPALDLID GTVVRLHQGD YGKQRDYGND PLPRLQDYAA QGAEVLHLVD
60 70 80 90 100
LTGAKDPAKR QIPLIKTLVA GVNVPVQVGG GVRSEEDVAA LLEAGVARVV
110 120 130 140 150
VGSTAVKSPE MVKGWFERFG ADALVLALDV RIDEQGNKQV AVSGWQENSG
160 170 180 190 200
VSLEQLVETY LPVGLKHVLC TDISRDGTLA GSNVSLYEEV CARYPQVAFQ
210 220 230 240
SSGGIGDIND VAALRGTGVR GVIVGRALLE GKFTVKEAIS CWQNA
Length:245
Mass (Da):26,031
Last modified:May 1, 2000 - v1
Checksum:i2CF26D4B4C032ADB
GO

Sequence cautioni

The sequence AAG57083.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAB36248.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB008676 Genomic DNA. Translation: BAA77741.1.
AE005174 Genomic DNA. Translation: AAG57083.1. Different initiation.
BA000007 Genomic DNA. Translation: BAB36248.1. Different initiation.
PIRiA98982.
G85827.
RefSeqiNP_288529.2. NC_002655.2.
NP_310852.2. NC_002695.1.

Genome annotation databases

EnsemblBacteriaiAAG57083; AAG57083; Z3186.
BAB36248; BAB36248; BAB36248.
GeneIDi912886.
962082.
KEGGiece:Z3186.
ecs:ECs2825.
PATRICi18355038. VBIEscCol44059_2720.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB008676 Genomic DNA. Translation: BAA77741.1 .
AE005174 Genomic DNA. Translation: AAG57083.1 . Different initiation.
BA000007 Genomic DNA. Translation: BAB36248.1 . Different initiation.
PIRi A98982.
G85827.
RefSeqi NP_288529.2. NC_002655.2.
NP_310852.2. NC_002695.1.

3D structure databases

ProteinModelPortali Q9S5G4.
SMRi Q9S5G4. Positions 2-242.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 155864.Z3186.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAG57083 ; AAG57083 ; Z3186 .
BAB36248 ; BAB36248 ; BAB36248 .
GeneIDi 912886.
962082.
KEGGi ece:Z3186.
ecs:ECs2825.
PATRICi 18355038. VBIEscCol44059_2720.

Phylogenomic databases

eggNOGi COG0106.
HOGENOMi HOG000224614.
KOi K01814.
OMAi TERIMYK.
OrthoDBi EOG6H1Q3W.

Enzyme and pathway databases

UniPathwayi UPA00031 ; UER00009 .
BioCyci ECOL386585:GJFA-2789-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01014. HisA.
InterProi IPR013785. Aldolase_TIM.
IPR006062. His_biosynth.
IPR006063. HisA.
IPR023016. Isoase_HisA.
IPR011060. RibuloseP-bd_barrel.
[Graphical view ]
Pfami PF00977. His_biosynth. 1 hit.
[Graphical view ]
SUPFAMi SSF51366. SSF51366. 1 hit.
TIGRFAMsi TIGR00007. TIGR00007. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of the genes responsible for the O-antigen synthesis in enterohaemorrhagic Escherichia coli O157."
    Shimizu T., Yamasaki S., Tsukamoto T., Takeda Y.
    Microb. Pathog. 26:235-247(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: O157:H- / 184 / EHEC.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
  3. "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
    Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.
    , Kuhara S., Shiba T., Hattori M., Shinagawa H.
    DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Entry informationi

Entry nameiHIS4_ECO57
AccessioniPrimary (citable) accession number: Q9S5G4
Secondary accession number(s): Q8X8S9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2002
Last sequence update: May 1, 2000
Last modified: October 29, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3