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Protein

Homogentisate 1,2-dioxygenase

Gene

hmgA

Organism
Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the catabolism of homogentisate (2,5-dihydroxyphenylacetate or 2,5-OH-PhAc), a central intermediate in the degradation of phenylalanine and tyrosine. Catalyzes the oxidative ring cleavage of the aromatic ring of homogentisate to yield maleylacetoacetate.UniRule annotation

Catalytic activityi

Homogentisate + O2 = 4-maleylacetoacetate.UniRule annotation

Cofactori

Fe cationUniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei275 – 2751Proton acceptorUniRule annotation
Metal bindingi318 – 3181IronUniRule annotation
Metal bindingi324 – 3241IronUniRule annotation
Binding sitei333 – 3331homogentisateUniRule annotation
Metal bindingi354 – 3541IronUniRule annotation
Binding sitei354 – 3541homogentisateUniRule annotation

GO - Molecular functioni

  1. homogentisate 1,2-dioxygenase activity Source: UniProtKB-HAMAP
  2. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. L-phenylalanine catabolic process Source: UniProtKB-HAMAP
  2. tyrosine catabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Phenylalanine catabolism, Tyrosine catabolism

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciLPNE272624:GHDI-1284-MONOMER.
UniPathwayiUPA00139; UER00339.

Names & Taxonomyi

Protein namesi
Recommended name:
Homogentisate 1,2-dioxygenaseUniRule annotation (EC:1.13.11.5UniRule annotation)
Short name:
HGDOUniRule annotation
Alternative name(s):
Homogentisate oxygenaseUniRule annotation
Homogentisic acid oxidaseUniRule annotation
HomogentisicaseUniRule annotation
Gene namesi
Name:hmgAUniRule annotation
Ordered Locus Names:lpg1285
OrganismiLegionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513)
Taxonomic identifieri272624 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaLegionellalesLegionellaceaeLegionella
ProteomesiUP000000609: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 416416Homogentisate 1,2-dioxygenasePRO_0000220248Add
BLAST

Interactioni

Subunit structurei

Hexamer; dimer of trimers.UniRule annotation

Protein-protein interaction databases

STRINGi272624.lpg1285.

Structurei

3D structure databases

ProteinModelPortaliQ9S4T0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the homogentisate dioxygenase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG3508.
HOGENOMiHOG000139824.
KOiK00451.
OMAiKSHFTPN.
OrthoDBiEOG6D5FZK.

Family and domain databases

Gene3Di2.60.120.10. 2 hits.
HAMAPiMF_00334. Homogentis_dioxygen.
InterProiIPR005708. Homogentis_dOase.
IPR022950. Homogentis_dOase_bac.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR11056. PTHR11056. 1 hit.
PfamiPF04209. HgmA. 1 hit.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.
TIGRFAMsiTIGR01015. hmgA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9S4T0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MYLQGFGNYH HSEAVKGALP PNQNSPQHCS LGLYAEQLSG TAFTRPRHNN
60 70 80 90 100
LRSWLYRILP TVTQGTYYPY EFNIMQPFVD ELSPNAMRWS PLYNSSQIKC
110 120 130 140 150
DFVEGLFHIA GSPLVNTYTY YCNHSMSDKY FANNDGELLF VPYAGEIHLH
160 170 180 190 200
TEFGKLMLSS GSIAVIPRGV KFKVEVISKE AKGYLCENSG NPLTLPQLGP
210 220 230 240 250
IGANGLANPR HFQYPVAAFE NSGSEHTIIC KNQKKLWFTV CNHSPLNVVA
260 270 280 290 300
WHGNYAPYCY DLSLFNTINT VSFDHPDPSI FTVLTSESEI PGVSNLDFVI
310 320 330 340 350
FPPRWMVAEH TFRPPYFHRN YMNELMGLVY GEYDAKKEGF IPGGISIHNC
360 370 380 390 400
MTPHGPDYES YEIAASQDLK PNYINSLAFM FETKDYWQVT EQAYRHPSRQ
410
MDYLNCWQGF KIEFSQ
Length:416
Mass (Da):47,475
Last modified:December 1, 2000 - v2
Checksum:i9E06F5AB9FFF3C9B
GO

Sequence cautioni

The sequence AAD51397.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF117715 Genomic DNA. Translation: AAD51397.1. Different initiation.
AE017354 Genomic DNA. Translation: AAU27368.1.
RefSeqiYP_095315.1. NC_002942.5.

Genome annotation databases

EnsemblBacteriaiAAU27368; AAU27368; lpg1285.
GeneIDi19832850.
KEGGilpn:lpg1285.
PATRICi22329658. VBILegPne29832_1353.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF117715 Genomic DNA. Translation: AAD51397.1. Different initiation.
AE017354 Genomic DNA. Translation: AAU27368.1.
RefSeqiYP_095315.1. NC_002942.5.

3D structure databases

ProteinModelPortaliQ9S4T0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272624.lpg1285.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAU27368; AAU27368; lpg1285.
GeneIDi19832850.
KEGGilpn:lpg1285.
PATRICi22329658. VBILegPne29832_1353.

Phylogenomic databases

eggNOGiCOG3508.
HOGENOMiHOG000139824.
KOiK00451.
OMAiKSHFTPN.
OrthoDBiEOG6D5FZK.

Enzyme and pathway databases

UniPathwayiUPA00139; UER00339.
BioCyciLPNE272624:GHDI-1284-MONOMER.

Family and domain databases

Gene3Di2.60.120.10. 2 hits.
HAMAPiMF_00334. Homogentis_dioxygen.
InterProiIPR005708. Homogentis_dOase.
IPR022950. Homogentis_dOase_bac.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PANTHERiPTHR11056. PTHR11056. 1 hit.
PfamiPF04209. HgmA. 1 hit.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.
TIGRFAMsiTIGR01015. hmgA. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The Legionella pneumophila rpoS gene is required for growth within Acanthamoeba castellanii."
    Hales L.M., Shuman H.A.
    J. Bacteriol. 181:4879-4889(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Philadelphia 1 / ATCC 33152 / DSM 7513.

Entry informationi

Entry nameiHGD_LEGPH
AccessioniPrimary (citable) accession number: Q9S4T0
Secondary accession number(s): Q5ZW03
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: February 4, 2015
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.