Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO

Gene

trmFO

Organism
Myxococcus xanthus (strain DK 1622)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + uracil(54) in tRNA + FADH2 = tetrahydrofolate + 5-methyluracil(54) in tRNA + FAD.UniRule annotation

Cofactori

FADUniRule annotation1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi12 – 17FADUniRule annotation6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processtRNA processing
LigandFAD, Flavoprotein

Enzyme and pathway databases

BioCyciMXAN246197:G1G53-2951-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOUniRule annotation (EC:2.1.1.74UniRule annotation)
Alternative name(s):
Folate-dependent tRNA (uracil-5-)-methyltransferaseUniRule annotation
Folate-dependent tRNA(M-5-U54)-methyltransferaseUniRule annotation
Gene namesi
Name:trmFOUniRule annotation
Synonyms:gid, gidA
Ordered Locus Names:MXAN_3007
OrganismiMyxococcus xanthus (strain DK 1622)
Taxonomic identifieri246197 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaMyxococcalesCystobacterineaeMyxococcaceaeMyxococcus
Proteomesi
  • UP000002402 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation1 Publication

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001172531 – 457Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOAdd BLAST457

Proteomic databases

PRIDEiQ9S449

Interactioni

Protein-protein interaction databases

STRINGi246197.MXAN_3007

Structurei

3D structure databases

ProteinModelPortaliQ9S449
SMRiQ9S449
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MnmG family. TrmFO subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107QXI Bacteria
COG1206 LUCA
HOGENOMiHOG000252054
KOiK04094
OMAiRFGPMKP
OrthoDBiPOG091H00B3

Family and domain databases

Gene3Di3.50.50.60, 2 hits
HAMAPiMF_01037 TrmFO, 1 hit
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR002218 MnmG-rel
IPR004417 TrmFO
PANTHERiPTHR11806 PTHR11806, 1 hit
PTHR11806:SF2 PTHR11806:SF2, 1 hit
PfamiView protein in Pfam
PF01134 GIDA, 1 hit
SUPFAMiSSF51905 SSF51905, 1 hit
TIGRFAMsiTIGR00137 gid_trmFO, 1 hit

Sequencei

Sequence statusi: Complete.

Q9S449-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADQKQRVTV IGGGLAGTEC AYQLSRRGVP VVLREMKPQK RSPAHKSDTL
60 70 80 90 100
AELVCSNSLR SDNPESAIGL LHAELRALGS LVLSAADANR VPAGDALAVE
110 120 130 140 150
RERFSAAITE SLLRQPGVEL VAGEVEQLPE DGPVVIATGP LTSDALTREL
160 170 180 190 200
ERHVGTRLYF YDSIAPILSA DSIDMNVAFR QSRYGKGGGD DYLNLPMTKD
210 220 230 240 250
EYYRFIAEVK AGQKVVPHAF EEPKYFEGCL PIEVMAERGD DTLAYGPMKP
260 270 280 290 300
VGLRDPRTGQ EPYAVVQLRM EDVGGTSWNM VGFQTRLTWG EQKRIFSSFI
310 320 330 340 350
PGLQQAEFLR MGQIHRNTFI DSPRLLAKDL SLKTEPRLYF AGQISGVEGY
360 370 380 390 400
VESAACGYLV ALALHARLTG TEFVPPPATT AMGALLRHVT GEAHPPDYPH
410 420 430 440 450
QPSNISFGIF SPLTGRMKKA EKRAAYSARA KQDLAAWLPH AGVPAAGAPE

HVDQRSA
Length:457
Mass (Da):49,764
Last modified:September 19, 2006 - v2
Checksum:iBE51C4E247353970
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti126 – 127EQ → DE in AAD46374 (PubMed:10844655).Curated2
Sequence conflicti225Y → S in AAD46374 (PubMed:10844655).Curated1
Sequence conflicti241D → N in AAD46374 (PubMed:10844655).Curated1
Sequence conflicti260Q → H in AAD46374 (PubMed:10844655).Curated1
Sequence conflicti269R → L in AAD46374 (PubMed:10844655).Curated1
Sequence conflicti314I → M in AAD46374 (PubMed:10844655).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF162663 Genomic DNA Translation: AAD46374.1
AY204472 Genomic DNA Translation: AAO22919.1
CP000113 Genomic DNA Translation: ABF92331.1
RefSeqiWP_011553063.1, NC_008095.1

Genome annotation databases

EnsemblBacteriaiABF92331; ABF92331; MXAN_3007
KEGGimxa:MXAN_3007

Entry informationi

Entry nameiTRMFO_MYXXD
AccessioniPrimary (citable) accession number: Q9S449
Secondary accession number(s): Q1D807, Q84F98
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2002
Last sequence update: September 19, 2006
Last modified: June 20, 2018
This is version 109 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health