Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO

Gene

trmFO

Organism
Myxococcus xanthus (strain DK 1622)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + uracil(54) in tRNA + FADH2 = tetrahydrofolate + 5-methyluracil(54) in tRNA + FAD.UniRule annotation

Cofactori

FADUniRule annotation1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi12 – 176FADUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciMXAN246197:GIWU-2980-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOUniRule annotation (EC:2.1.1.74UniRule annotation)
Alternative name(s):
Folate-dependent tRNA (uracil-5-)-methyltransferaseUniRule annotation
Folate-dependent tRNA(M-5-U54)-methyltransferaseUniRule annotation
Gene namesi
Name:trmFOUniRule annotation
Synonyms:gid, gidA
Ordered Locus Names:MXAN_3007
OrganismiMyxococcus xanthus (strain DK 1622)
Taxonomic identifieri246197 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaMyxococcalesCystobacterineaeMyxococcaceaeMyxococcus
Proteomesi
  • UP000002402 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 457457Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOPRO_0000117253Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi246197.MXAN_3007.

Structurei

3D structure databases

ProteinModelPortaliQ9S449.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MnmG family. TrmFO subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107QXI. Bacteria.
COG1206. LUCA.
HOGENOMiHOG000252054.
KOiK04094.
OMAiDYLNCPM.
OrthoDBiPOG091H00B3.

Family and domain databases

Gene3Di3.50.50.60. 5 hits.
HAMAPiMF_01037. TrmFO. 1 hit.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR002218. MnmG-rel.
IPR004417. TrmFO.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9S449-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADQKQRVTV IGGGLAGTEC AYQLSRRGVP VVLREMKPQK RSPAHKSDTL
60 70 80 90 100
AELVCSNSLR SDNPESAIGL LHAELRALGS LVLSAADANR VPAGDALAVE
110 120 130 140 150
RERFSAAITE SLLRQPGVEL VAGEVEQLPE DGPVVIATGP LTSDALTREL
160 170 180 190 200
ERHVGTRLYF YDSIAPILSA DSIDMNVAFR QSRYGKGGGD DYLNLPMTKD
210 220 230 240 250
EYYRFIAEVK AGQKVVPHAF EEPKYFEGCL PIEVMAERGD DTLAYGPMKP
260 270 280 290 300
VGLRDPRTGQ EPYAVVQLRM EDVGGTSWNM VGFQTRLTWG EQKRIFSSFI
310 320 330 340 350
PGLQQAEFLR MGQIHRNTFI DSPRLLAKDL SLKTEPRLYF AGQISGVEGY
360 370 380 390 400
VESAACGYLV ALALHARLTG TEFVPPPATT AMGALLRHVT GEAHPPDYPH
410 420 430 440 450
QPSNISFGIF SPLTGRMKKA EKRAAYSARA KQDLAAWLPH AGVPAAGAPE

HVDQRSA
Length:457
Mass (Da):49,764
Last modified:September 19, 2006 - v2
Checksum:iBE51C4E247353970
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti126 – 1272EQ → DE in AAD46374 (PubMed:10844655).Curated
Sequence conflicti225 – 2251Y → S in AAD46374 (PubMed:10844655).Curated
Sequence conflicti241 – 2411D → N in AAD46374 (PubMed:10844655).Curated
Sequence conflicti260 – 2601Q → H in AAD46374 (PubMed:10844655).Curated
Sequence conflicti269 – 2691R → L in AAD46374 (PubMed:10844655).Curated
Sequence conflicti314 – 3141I → M in AAD46374 (PubMed:10844655).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF162663 Genomic DNA. Translation: AAD46374.1.
AY204472 Genomic DNA. Translation: AAO22919.1.
CP000113 Genomic DNA. Translation: ABF92331.1.
RefSeqiWP_011553063.1. NC_008095.1.

Genome annotation databases

EnsemblBacteriaiABF92331; ABF92331; MXAN_3007.
KEGGimxa:MXAN_3007.
PATRICi22648573. VBIMyxXan43560_2967.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF162663 Genomic DNA. Translation: AAD46374.1.
AY204472 Genomic DNA. Translation: AAO22919.1.
CP000113 Genomic DNA. Translation: ABF92331.1.
RefSeqiWP_011553063.1. NC_008095.1.

3D structure databases

ProteinModelPortaliQ9S449.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246197.MXAN_3007.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABF92331; ABF92331; MXAN_3007.
KEGGimxa:MXAN_3007.
PATRICi22648573. VBIMyxXan43560_2967.

Phylogenomic databases

eggNOGiENOG4107QXI. Bacteria.
COG1206. LUCA.
HOGENOMiHOG000252054.
KOiK04094.
OMAiDYLNCPM.
OrthoDBiPOG091H00B3.

Enzyme and pathway databases

BioCyciMXAN246197:GIWU-2980-MONOMER.

Family and domain databases

Gene3Di3.50.50.60. 5 hits.
HAMAPiMF_01037. TrmFO. 1 hit.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR002218. MnmG-rel.
IPR004417. TrmFO.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTRMFO_MYXXD
AccessioniPrimary (citable) accession number: Q9S449
Secondary accession number(s): Q1D807, Q84F98
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2002
Last sequence update: September 19, 2006
Last modified: September 7, 2016
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.