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Q9S424 (BLA13_KLEPN) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-lactamase SHV-13

EC=3.5.2.6
Gene names
Name:bla
Synonyms:shv13
Encoded onPlasmid
OrganismKlebsiella pneumoniae
Taxonomic identifier573 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeKlebsiella

Protein attributes

Sequence length286 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Broad spectrum beta-lactamase which hydrolyzes penicillins, as well as cephalosporins except cephamycins. Also hydrolyzes aztreonam, but not imipenem. Confers highly resistance to ceftazidime, cefotaxime, aztreonam and piperacillin.

Catalytic activity

A beta-lactam + H2O = a substituted beta-amino acid.

Enzyme regulation

Inhibited 16-fold better by the beta-lactamase inhibitor clavulanic acid than by tazobactam.

Sequence similarities

Belongs to the class-A beta-lactamase family.

Ontologies

Keywords
   Biological processAntibiotic resistance
   DomainSignal
   Molecular functionHydrolase
   PTMDisulfide bond
   Technical termPlasmid
Gene Ontology (GO)
   Biological_processbeta-lactam antibiotic catabolic process

Inferred from electronic annotation. Source: InterPro

response to antibiotic

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionbeta-lactamase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Potential
Chain22 – 286265Beta-lactamase SHV-13
PRO_0000016987

Regions

Region230 – 2323Substrate binding By similarity

Sites

Active site661Acyl-ester intermediate By similarity
Active site1641Proton acceptor By similarity

Amino acid modifications

Disulfide bond73 ↔ 119 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9S424 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: CBBF426DA3FF5502

FASTA28631,253
        10         20         30         40         50         60 
MRYIRLCIIS LLATLPLAVH ASPQPLEQIK QSESQLSGRV GMIEMDLASG RTLTAWRADE 

        70         80         90        100        110        120 
RFPMMSTFKV VLCGAVLARV DAGDEQLERK IHYRQQDLVD YSPVSEKHLA DGMTVGELCA 

       130        140        150        160        170        180 
AAITMSDNSA ANLLLATVGG PAGLTAFLRQ IGDNVTRLDR WETELNEALP GDARDTTTPA 

       190        200        210        220        230        240 
SMAATLRKLL TSQRLSARSQ RQLLQWMVDD RVAGPLIRSV LPAGWFIADK TGAAERGARG 

       250        260        270        280 
IVALLGPNNK AERIVVIYLR DTPASMAERN QQIAGIGAAL IEHWQR 

« Hide

References

[1]"SHV-13, a novel extended-spectrum beta-lactamase, in Klebsiella pneumoniae isolates from patients in an intensive care unit in Amsterdam."
Yuan M., Hall L.M.C., Savelkoul P.H.M., Vandenbroucke-Grauls C.M.J.E., Livermore D.M.
Antimicrob. Agents Chemother. 44:1081-1084(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 803.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF164577 Genomic DNA. Translation: AAD43815.1.

3D structure databases

ProteinModelPortalQ9S424.
SMRQ9S424. Positions 22-286.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.710.10. 1 hit.
InterProIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PRINTSPR00118. BLACTAMASEA.
SUPFAMSSF56601. SSF56601. 1 hit.
PROSITEPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBLA13_KLEPN
AccessionPrimary (citable) accession number: Q9S424
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 2000
Last modified: October 16, 2013
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families