Reviewed, UniProtKB/Swiss-Prot Q9S424 (BLA13_KLEPN)

Names and origin

Protein names

Recommended name:
    Beta-lactamase SHV-13
    EC=3.5.2.6

Gene names

Name:	bla
Synonyms:	shv13

Encoded on

Plasmid

Organism

Klebsiella pneumoniae

Taxonomic identifier

573 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella

Protein attributes

Sequence length	286 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Broad spectrum beta-lactamase which hydrolyzes penicillins, as well as cephalosporins except cephamycins. Also hydrolyzes aztreonam, but not imipenem. Confers highly resistance to ceftazidime, cefotaxime, aztreonam and piperacillin.
Catalytic activity	A beta-lactam + H2O = a substituted beta-amino acid.
Enzyme regulation	Inhibited 16-fold better by the beta-lactamase inhibitor clavulanic acid than by tazobactam.
Sequence similarities	Belongs to the class-A beta-lactamase family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Domain	Signal
Molecular function	Hydrolase
PTM	Disulfide bond
Technical term	Plasmid
Gene Ontology (GO)
Biological process	beta-lactam antibiotic catabolic process Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	beta-lactamase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 286	265	Beta-lactamase SHV-13		PRO_0000016987
Regions
	Region	230 – 232	3	Substrate binding By similarity
Sites
	Active site	66	1	Acyl-ester intermediate By similarity
	Active site	164	1	Proton acceptor By similarity
Amino acid modifications
	Disulfide bond	73 ↔ 119		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9S424-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: CBBF426DA3FF5502	FASTA	286	31,253
	10 20 30 40 50 60 MRYIRLCIIS LLATLPLAVH ASPQPLEQIK QSESQLSGRV GMIEMDLASG RTLTAWRADE 70 80 90 100 110 120 RFPMMSTFKV VLCGAVLARV DAGDEQLERK IHYRQQDLVD YSPVSEKHLA DGMTVGELCA 130 140 150 160 170 180 AAITMSDNSA ANLLLATVGG PAGLTAFLRQ IGDNVTRLDR WETELNEALP GDARDTTTPA 190 200 210 220 230 240 SMAATLRKLL TSQRLSARSQ RQLLQWMVDD RVAGPLIRSV LPAGWFIADK TGAAERGARG 250 260 270 280 IVALLGPNNK AERIVVIYLR DTPASMAERN QQIAGIGAAL IEHWQR

References

[1]

"SHV-13, a novel extended-spectrum beta-lactamase, in Klebsiella pneumoniae isolates from patients in an intensive care unit in Amsterdam." Yuan M., Hall L.M.C., Savelkoul P.H.M., Vandenbroucke-Grauls C.M.J.E., Livermore D.M. Antimicrob. Agents Chemother. 44:1081-1084(2000) [PubMed: 10722518] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 803.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF164577 Genomic DNA. Translation: AAD43815.1.
3D structure databases
SMR	Q9S424. Positions 22-286.
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.5.2.6. 758.
Family and domain databases
InterPro	IPR012338. B-lactamase-typ_transpept_fold. IPR001466. Beta_lactamase-related. IPR000871. Beta_lactamase_A/D. [Graphical view]
Pfam	PF00144. Beta-lactamase. 1 hit. [Graphical view]
PRINTS	PR00118. BLACTAMASEA.
PROSITE	PS00146. BETA_LACTAMASE_A. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BLA13_KLEPN

Accession

Primary (citable) accession number: Q9S424

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 2000
Last modified:	November 24, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)