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Protein

Beta-lactamase SHV-13

Gene

bla

Organism
Klebsiella pneumoniae
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Broad spectrum beta-lactamase which hydrolyzes penicillins, as well as cephalosporins except cephamycins. Also hydrolyzes aztreonam, but not imipenem. Confers highly resistance to ceftazidime, cefotaxime, aztreonam and piperacillin.

Catalytic activityi

A beta-lactam + H2O = a substituted beta-amino acid.PROSITE-ProRule annotation

Enzyme regulationi

Inhibited 16-fold better by the beta-lactamase inhibitor clavulanic acid than by tazobactam.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei66Acyl-ester intermediatePROSITE-ProRule annotation1
Active sitei164Proton acceptorBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Antibiotic resistance

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-lactamase SHV-13 (EC:3.5.2.6)
Gene namesi
Name:bla
Synonyms:shv13
Encoded oniPlasmid0 Publication
OrganismiKlebsiella pneumoniae
Taxonomic identifieri573 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeKlebsiella

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000001698722 – 286Beta-lactamase SHV-13Add BLAST265

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi73 ↔ 119By similarity

Keywords - PTMi

Disulfide bond

Interactioni

Protein-protein interaction databases

STRINGi272620.KPN_01607.

Structurei

3D structure databases

ProteinModelPortaliQ9S424.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni230 – 232Substrate bindingBy similarity3

Sequence similaritiesi

Belongs to the class-A beta-lactamase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4108J4B. Bacteria.
COG2367. LUCA.
KOiK18699.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9S424-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRYIRLCIIS LLATLPLAVH ASPQPLEQIK QSESQLSGRV GMIEMDLASG
60 70 80 90 100
RTLTAWRADE RFPMMSTFKV VLCGAVLARV DAGDEQLERK IHYRQQDLVD
110 120 130 140 150
YSPVSEKHLA DGMTVGELCA AAITMSDNSA ANLLLATVGG PAGLTAFLRQ
160 170 180 190 200
IGDNVTRLDR WETELNEALP GDARDTTTPA SMAATLRKLL TSQRLSARSQ
210 220 230 240 250
RQLLQWMVDD RVAGPLIRSV LPAGWFIADK TGAAERGARG IVALLGPNNK
260 270 280
AERIVVIYLR DTPASMAERN QQIAGIGAAL IEHWQR
Length:286
Mass (Da):31,253
Last modified:May 1, 2000 - v1
Checksum:iCBBF426DA3FF5502
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF164577 Genomic DNA. Translation: AAD43815.1.
RefSeqiWP_063864619.1. NG_050008.1.

Genome annotation databases

KEGGiag:AAD43815.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF164577 Genomic DNA. Translation: AAD43815.1.
RefSeqiWP_063864619.1. NG_050008.1.

3D structure databases

ProteinModelPortaliQ9S424.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272620.KPN_01607.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:AAD43815.

Phylogenomic databases

eggNOGiENOG4108J4B. Bacteria.
COG2367. LUCA.
KOiK18699.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PRINTSiPR00118. BLACTAMASEA.
SUPFAMiSSF56601. SSF56601. 1 hit.
PROSITEiPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBLA13_KLEPN
AccessioniPrimary (citable) accession number: Q9S424
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 2000
Last modified: November 2, 2016
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Plasmid

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.