SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q9S2X5

- SYI_STRCO

UniProt

Q9S2X5 - SYI_STRCO

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Isoleucine--tRNA ligase

Gene
ileS, SCO2076, SC4A10.09
Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei603 – 6031ATP By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:SCO2076
ORF Names:SC4A10.09
OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Taxonomic identifieri100226 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
ProteomesiUP000001973: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10471047Isoleucine--tRNA ligaseUniRule annotationPRO_0000098564Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi100226.SCO2076.

Structurei

3D structure databases

ProteinModelPortaliQ9S2X5.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi52 – 6211"HIGH" regionUniRule annotationAdd
BLAST
Motifi600 – 6045"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiERLMLHQ.
OrthoDBiEOG644ZM1.
PhylomeDBiQ9S2X5.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9S2X5-1 [UniParc]FASTAAdd to Basket

« Hide

MTTPQYRQVP AQVDLPALEH AVLDFWREQK IFAKSLEQSE GRPEWVFYEG     50
PPTANGMPGA HHIEARVFKD VFPRFRTMRG YHVGRKAGWD CHGLPVELAV 100
EKELGFSGKQ DIEAYGIAEF NAKCRESVTR HTDAFEELTT RMGYWADLQD 150
PYRTMDPEYI ESVWWSLKEI FNKGLLVQDH RVAPWCPRCG TGLSDHELAQ 200
GYETVVDPSV YVRFPLTSGP LAGEAALVVW TTTPWTLVSN TAVAAHPDVT 250
YVVATDGEEK LVVAEPLLAK ALGEGWETTG QSFTGAEMER WTYQRPFELV 300
EFPEPAHYVV NADYVTTEDG TGLVHQSPAF GEDDLKVCRA YGLPVVNPVR 350
PDGTFEEDVP LVGGVFFKKA DEKLTEDLET RGLLFKHIPY EHSYPHCWRC 400
HTALLYYAQP SWYIRTTAIK DRLLQENEKT NWFPDAVKHG RYGDWLNNNI 450
DWALSRNRYW GTPLPIWRCA EDHLTVVGSR AELTELSGTD QSSLDPHRPF 500
IDDVTFTCAQ EGCSLEAVRV PEVIDAWYDS GSMPFAQWGY PYKNKELFES 550
RYPAQFISEA IDQTRGWFYT LMAVGTLVFD KSSYENVVCL GHILAEDGRK 600
MSKHLGNILQ PIPLMDQHGA DAVRWFMAAG GSPWAARRVG HGTIQEVVRK 650
TLLTYWNTVA FQALYARTTG WAPSEADPAP ADRPVLDRWL LSELHALTDQ 700
VTQALDAYDT QRAGKLLSAF VDDLSNWYVR RSRRRFWQGD KAALRTLHEV 750
VETVTKLMAP LTPFITERVW QDLVVPVTPG APESVHLSSW PEADLTAIDP 800
ELSKQMVLVR RLVELGRATR AESGVKTRQP LSRALIAVAG FDTLSPELHS 850
QITEELNVAS LASLSEVGGS LVDTTAKANF RALGKRFGKR VQDVAKAVAA 900
ADAAALSLAL REGTASVEVD GETVTLAPDE VIITETPREG WSVASDSGAT 950
VALDLELTEE LRRAGLARDA IRLIQEARKN SGLDVADRIA LRWTATDPAT 1000
IAALTDHSGL ISDEVLATDF AQGEADDSYG APFTDEGLSL VFRLRKQ 1047
Length:1,047
Mass (Da):116,886
Last modified:May 1, 2000 - v1
Checksum:i288C99247ADA7666
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL939111 Genomic DNA. Translation: CAB51985.1.
PIRiT34946.
RefSeqiNP_626335.1. NC_003888.3.

Genome annotation databases

EnsemblBacteriaiCAB51985; CAB51985; CAB51985.
GeneIDi1097510.
KEGGisco:SCO2076.
PATRICi23733800. VBIStrCoe124346_2109.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL939111 Genomic DNA. Translation: CAB51985.1 .
PIRi T34946.
RefSeqi NP_626335.1. NC_003888.3.

3D structure databases

ProteinModelPortali Q9S2X5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 100226.SCO2076.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAB51985 ; CAB51985 ; CAB51985 .
GeneIDi 1097510.
KEGGi sco:SCO2076.
PATRICi 23733800. VBIStrCoe124346_2109.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi ERLMLHQ.
OrthoDBi EOG644ZM1.
PhylomeDBi Q9S2X5.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-471 / A3(2) / M145.

Entry informationi

Entry nameiSYI_STRCO
AccessioniPrimary (citable) accession number: Q9S2X5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: May 1, 2000
Last modified: May 14, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi