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Q9S2X5

- SYI_STRCO

UniProt

Q9S2X5 - SYI_STRCO

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 77 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Zinc.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei603 – 6031ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:SCO2076
    ORF Names:SC4A10.09
    OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
    Taxonomic identifieri100226 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
    ProteomesiUP000001973: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10471047Isoleucine--tRNA ligasePRO_0000098564Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi100226.SCO2076.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9S2X5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi52 – 6211"HIGH" regionAdd
    BLAST
    Motifi600 – 6045"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246403.
    KOiK01870.
    OMAiERLMLHQ.
    OrthoDBiEOG644ZM1.
    PhylomeDBiQ9S2X5.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9S2X5-1 [UniParc]FASTAAdd to Basket

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    MTTPQYRQVP AQVDLPALEH AVLDFWREQK IFAKSLEQSE GRPEWVFYEG     50
    PPTANGMPGA HHIEARVFKD VFPRFRTMRG YHVGRKAGWD CHGLPVELAV 100
    EKELGFSGKQ DIEAYGIAEF NAKCRESVTR HTDAFEELTT RMGYWADLQD 150
    PYRTMDPEYI ESVWWSLKEI FNKGLLVQDH RVAPWCPRCG TGLSDHELAQ 200
    GYETVVDPSV YVRFPLTSGP LAGEAALVVW TTTPWTLVSN TAVAAHPDVT 250
    YVVATDGEEK LVVAEPLLAK ALGEGWETTG QSFTGAEMER WTYQRPFELV 300
    EFPEPAHYVV NADYVTTEDG TGLVHQSPAF GEDDLKVCRA YGLPVVNPVR 350
    PDGTFEEDVP LVGGVFFKKA DEKLTEDLET RGLLFKHIPY EHSYPHCWRC 400
    HTALLYYAQP SWYIRTTAIK DRLLQENEKT NWFPDAVKHG RYGDWLNNNI 450
    DWALSRNRYW GTPLPIWRCA EDHLTVVGSR AELTELSGTD QSSLDPHRPF 500
    IDDVTFTCAQ EGCSLEAVRV PEVIDAWYDS GSMPFAQWGY PYKNKELFES 550
    RYPAQFISEA IDQTRGWFYT LMAVGTLVFD KSSYENVVCL GHILAEDGRK 600
    MSKHLGNILQ PIPLMDQHGA DAVRWFMAAG GSPWAARRVG HGTIQEVVRK 650
    TLLTYWNTVA FQALYARTTG WAPSEADPAP ADRPVLDRWL LSELHALTDQ 700
    VTQALDAYDT QRAGKLLSAF VDDLSNWYVR RSRRRFWQGD KAALRTLHEV 750
    VETVTKLMAP LTPFITERVW QDLVVPVTPG APESVHLSSW PEADLTAIDP 800
    ELSKQMVLVR RLVELGRATR AESGVKTRQP LSRALIAVAG FDTLSPELHS 850
    QITEELNVAS LASLSEVGGS LVDTTAKANF RALGKRFGKR VQDVAKAVAA 900
    ADAAALSLAL REGTASVEVD GETVTLAPDE VIITETPREG WSVASDSGAT 950
    VALDLELTEE LRRAGLARDA IRLIQEARKN SGLDVADRIA LRWTATDPAT 1000
    IAALTDHSGL ISDEVLATDF AQGEADDSYG APFTDEGLSL VFRLRKQ 1047
    Length:1,047
    Mass (Da):116,886
    Last modified:May 1, 2000 - v1
    Checksum:i288C99247ADA7666
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL939111 Genomic DNA. Translation: CAB51985.1.
    PIRiT34946.
    RefSeqiNP_626335.1. NC_003888.3.

    Genome annotation databases

    EnsemblBacteriaiCAB51985; CAB51985; CAB51985.
    GeneIDi1097510.
    KEGGisco:SCO2076.
    PATRICi23733800. VBIStrCoe124346_2109.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL939111 Genomic DNA. Translation: CAB51985.1 .
    PIRi T34946.
    RefSeqi NP_626335.1. NC_003888.3.

    3D structure databases

    ProteinModelPortali Q9S2X5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 100226.SCO2076.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB51985 ; CAB51985 ; CAB51985 .
    GeneIDi 1097510.
    KEGGi sco:SCO2076.
    PATRICi 23733800. VBIStrCoe124346_2109.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246403.
    KOi K01870.
    OMAi ERLMLHQ.
    OrthoDBi EOG644ZM1.
    PhylomeDBi Q9S2X5.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-471 / A3(2) / M145.

    Entry informationi

    Entry nameiSYI_STRCO
    AccessioniPrimary (citable) accession number: Q9S2X5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 77 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3