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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei603ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:SCO2076
ORF Names:SC4A10.09
OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Taxonomic identifieri100226 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
Proteomesi
  • UP000001973 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000985641 – 1047Isoleucine--tRNA ligaseAdd BLAST1047

Proteomic databases

PRIDEiQ9S2X5

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi100226.SCO2076

Structurei

3D structure databases

ProteinModelPortaliQ9S2X5
SMRiQ9S2X5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi52 – 62"HIGH" regionAdd BLAST11
Motifi600 – 604"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
HOGENOMiHOG000246403
InParanoidiQ9S2X5
KOiK01870
OMAiPSWYIRT
OrthoDBiPOG091H028I
PhylomeDBiQ9S2X5

Family and domain databases

CDDicd07961 Anticodon_Ia_Ile_ABEc, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02003 Ile_tRNA_synth_type2, 1 hit
InterProiView protein in InterPro
IPR002300 aa-tRNA-synth_Ia
IPR033709 Anticodon_Ile_ABEc
IPR002301 Ile-tRNA-ligase
IPR023586 Ile-tRNA-ligase_type2
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit

Sequencei

Sequence statusi: Complete.

Q9S2X5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTPQYRQVP AQVDLPALEH AVLDFWREQK IFAKSLEQSE GRPEWVFYEG
60 70 80 90 100
PPTANGMPGA HHIEARVFKD VFPRFRTMRG YHVGRKAGWD CHGLPVELAV
110 120 130 140 150
EKELGFSGKQ DIEAYGIAEF NAKCRESVTR HTDAFEELTT RMGYWADLQD
160 170 180 190 200
PYRTMDPEYI ESVWWSLKEI FNKGLLVQDH RVAPWCPRCG TGLSDHELAQ
210 220 230 240 250
GYETVVDPSV YVRFPLTSGP LAGEAALVVW TTTPWTLVSN TAVAAHPDVT
260 270 280 290 300
YVVATDGEEK LVVAEPLLAK ALGEGWETTG QSFTGAEMER WTYQRPFELV
310 320 330 340 350
EFPEPAHYVV NADYVTTEDG TGLVHQSPAF GEDDLKVCRA YGLPVVNPVR
360 370 380 390 400
PDGTFEEDVP LVGGVFFKKA DEKLTEDLET RGLLFKHIPY EHSYPHCWRC
410 420 430 440 450
HTALLYYAQP SWYIRTTAIK DRLLQENEKT NWFPDAVKHG RYGDWLNNNI
460 470 480 490 500
DWALSRNRYW GTPLPIWRCA EDHLTVVGSR AELTELSGTD QSSLDPHRPF
510 520 530 540 550
IDDVTFTCAQ EGCSLEAVRV PEVIDAWYDS GSMPFAQWGY PYKNKELFES
560 570 580 590 600
RYPAQFISEA IDQTRGWFYT LMAVGTLVFD KSSYENVVCL GHILAEDGRK
610 620 630 640 650
MSKHLGNILQ PIPLMDQHGA DAVRWFMAAG GSPWAARRVG HGTIQEVVRK
660 670 680 690 700
TLLTYWNTVA FQALYARTTG WAPSEADPAP ADRPVLDRWL LSELHALTDQ
710 720 730 740 750
VTQALDAYDT QRAGKLLSAF VDDLSNWYVR RSRRRFWQGD KAALRTLHEV
760 770 780 790 800
VETVTKLMAP LTPFITERVW QDLVVPVTPG APESVHLSSW PEADLTAIDP
810 820 830 840 850
ELSKQMVLVR RLVELGRATR AESGVKTRQP LSRALIAVAG FDTLSPELHS
860 870 880 890 900
QITEELNVAS LASLSEVGGS LVDTTAKANF RALGKRFGKR VQDVAKAVAA
910 920 930 940 950
ADAAALSLAL REGTASVEVD GETVTLAPDE VIITETPREG WSVASDSGAT
960 970 980 990 1000
VALDLELTEE LRRAGLARDA IRLIQEARKN SGLDVADRIA LRWTATDPAT
1010 1020 1030 1040
IAALTDHSGL ISDEVLATDF AQGEADDSYG APFTDEGLSL VFRLRKQ
Length:1,047
Mass (Da):116,886
Last modified:May 1, 2000 - v1
Checksum:i288C99247ADA7666
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL939111 Genomic DNA Translation: CAB51985.1
PIRiT34946
RefSeqiNP_626335.1, NC_003888.3
WP_003976739.1, NC_003888.3

Genome annotation databases

EnsemblBacteriaiCAB51985; CAB51985; CAB51985
GeneIDi1097510
KEGGisco:SCO2076
PATRICifig|100226.15.peg.2109

Similar proteinsi

Entry informationi

Entry nameiSYI_STRCO
AccessioniPrimary (citable) accession number: Q9S2X5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: May 1, 2000
Last modified: May 23, 2018
This is version 102 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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