Q9S2W9 (MURD_STRCO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylmuramoylalanine--D-glutamate ligase
EC=6.3.2.9

Alternative name(s):
D-glutamic acid-adding enzyme
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Gene names

Name:	murD
Ordered Locus Names:	SCO2086
ORF Names:	SC4A10.19c

Organism

Streptomyces coelicolor

Taxonomic identifier

1902 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	471 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) By similarity. HAMAP MF_00639
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate. HAMAP MF_00639
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00639
Subcellular location	Cytoplasm By similarity HAMAP MF_00639.
Sequence similarities	Belongs to the MurCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW UDP-N-acetylmuramoylalanine-D-glutamate ligase activity Inferred from electronic annotation. Source: EC tetrahydrofolylpolyglutamate synthase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 471	471	UDP-N-acetylmuramoylalanine--D-glutamate ligase HAMAP MF_00639		PRO_0000109106
Regions
	Nucleotide binding	122 – 128	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9S2W9 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: EF5A042077CBD9F3	FASTA	471	49,143
	10 20 30 40 50 60 MPSEFSGKHV TVAGLGVSGV PAAKVLHGLG AQVTVVNDGD DERARTQAAE LEPLGVTVRL 70 80 90 100 110 120 GDGDTLPEGT ELIVTAPGWK PTKPLFTAAG QAGVPVWGDV ELAWRLRGLN GRKPAPWLAV 130 140 150 160 170 180 TGTNGKTTTV QMLASILKAA GLRTAAVGNI GVSLLDAVTG EQEYDVLAVE LSSYQLHWAP 190 200 210 220 230 240 SLRAHSAAVL NLAPDHLDWH GSMEAYAADK GRIYEGNHVA CVYNVADKAT EDLVRAADVE 250 260 270 280 290 300 EGCRAIGFTL GTPGPSQLGV VEGLLVDRAF VEDRQKNAQE LAEVSDVNPP APHNIANALA 310 320 330 340 350 360 AAGLARAFGV SAAAVRDGLR AFTPDAHRIA HVADVDGVAY VDDSKATNTH ATEASLAAYE 370 380 390 400 410 420 SIVWIAGGLA KGATFDELVA GAAKRLRGAV LIGADRALIR EALARHAPEV PVVDLDRTDT 430 440 450 460 470 GAMLQAVQEA RRLARPGDTV LLAPACASMD MFTNYNQRGD AFAQAVRELG A

References

[1]

"Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2)." Bentley S.D., Chater K.F., Cerdeno-Tarraga A.-M., Challis G.L., Thomson N.R., James K.D., Harris D.E., Quail M.A., Kieser H., Harper D., Bateman A., Brown S., Chandra G., Chen C.W., Collins M., Cronin A., Fraser A., Goble A. , Hidalgo J., Hornsby T., Howarth S., Huang C.-H., Kieser T., Larke L., Murphy L.D., Oliver K., O'Neil S., Rabbinowitsch E., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Saunders D., Sharp S., Squares R., Squares S., Taylor K., Warren T., Wietzorrek A., Woodward J.R., Barrell B.G., Parkhill J., Hopwood D.A. Nature 417:141-147(2002) [PubMed: 12000953] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-471 / A3(2) / M145.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL939111 Genomic DNA. Translation: CAB51995.1.
PIR	T34956.
RefSeq	NP_626345.1. NC_003888.3.
3D structure databases
ProteinModelPortal	Q9S2W9.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1097520.
GenomeReviews	Gene locus SCO2086 in contig AL645882_GR.
KEGG	sco:SCO2086.
NMPDR	fig|100226.1.peg.2052.
PATRIC	23733820. VBIStrCoe124346_2119.
Phylogenomic databases
HOGENOM	HBG750024.
OMA	ACASWDM.
PhylomeDB	Q9S2W9.
ProtClustDB	PRK01438.
Family and domain databases
HAMAP	MF_00639. MurD. [Tree]
InterPro	IPR018109. Folylpolyglutamate_synth_CS. IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR016040. NAD(P)-bd_dom. IPR005762. UDP-N-AcMur-Glu_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K01925.
PANTHER	PTHR23135:SF2. PTHR23135:SF2. 1 hit.
Pfam	PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
SUPFAM	SSF53244. Mur_ligase_C. 1 hit. SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMs	TIGR01087. MurD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURD_STRCO

Accession

Primary (citable) accession number: Q9S2W9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 30, 2003
Last sequence update:	May 1, 2000
Last modified:	January 25, 2012
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program