Q9S1H0 (SERA_THASE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Selenate reductase subunit alpha
EC=1.97.1.9

Alternative name(s):
Selenate reductase molybdenum subunit

Gene names

Name:

serA

Organism

Thauera selenatis

Taxonomic identifier

33058 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Thauera

Protein attributes

Sequence length	918 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Terminal reductase that allows anaerobic growth on selenate as the sole respiratory oxidant.
Catalytic activity	Selenite + H2O + acceptor = selenate + reduced acceptor.
Cofactor	Binds 1 4Fe-4S cluster Potential. Molybdenum (molybdopterin).
Enzyme regulation	Inhibited by O2.
Subunit structure	Heterotrimer of alpha, beta and gamma subunits.
Subcellular location	Periplasm.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has been experimentally proven.
Biotechnological use	Has potential use in bioremediation of waste sites contaminated with selenate, such as agricultural drainage waters.
Sequence similarities	Belongs to the prokaryotic molybdopterin-containing oxidoreductase family.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Signal
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding Molybdenum
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular_component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW molybdenum ion binding Inferred from electronic annotation. Source: InterPro selenate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 36	36	Tat-type signal Ref.2
	Chain	37 – 918	882	Selenate reductase subunit alpha		PRO_0000019175
Sites
	Metal binding	73	1	Iron-sulfur (4Fe-4S); via pros nitrogen By similarity
	Metal binding	77	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	81	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	115	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9S1H0 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 995D6134B107798F	FASTA	918	103,407
	10 20 30 40 50 60 MRKVMNSPDD GNGRRRFLQF SMAALASAAA PSSVWAFSKI QPIEDPLKSY PYRDWEDLYR 70 80 90 100 110 120 KEWTWDSTGF ITHSNGCVAG CAWRVFVKNG VPMREEQVSE YPQLPGVPDM NPRGCQKGAV 130 140 150 160 170 180 YCSWSKQPDF LKYPLKRVGE RGERKWKRIS WDEAFTEIAD KIIDTTVKRG PGNVCMPKRP 190 200 210 220 230 240 FAVITSAGYS RLANLIGAIK PDVSSMTGDL YPGIQTVRMP ARTVSTFDDW FTSDLILMWH 250 260 270 280 290 300 KNPIVTRIPD AHFLTEARYN GARLVNISPD YNPSSVHADL HLPVTTGTDS HLAAAIVNVL 310 320 330 340 350 360 IADKKYKADY LKEQTDLPFL VRTDNGKFLR EKDFNKDGSD EVFYIWDSKS GKAVLAPGSM 370 380 390 400 410 420 GSKDKTLKLG AVEPALEGTF DANGIEVTTV FARLKAEIAP YTPEATHKTT GIHPSVVRQL 430 440 450 460 470 480 AGWIGDCKAL RILDGYNNQK HFDGFQCGRL KILILTLIGH HGTTGSIDTT YEGWVLEGNK 490 500 510 520 530 540 ALGGVKGRPG RSVSMVLAQW VWGEQYRRSK AYFDDTELRE QIGFGVDEME ALRKESEANG 550 560 570 580 590 600 WMPNWQSIKD PVVYINAGIN TFATSTGYQH LRENFLKRCE LYVVVDFRLN SGAMYADIVL 610 620 630 640 650 660 PAATNLEKLD IRETSSTRFI HAFGQPIKPM YDRRTDWQIS VGLARKIQER ARARGITRVD 670 680 690 700 710 720 DPEIKSFIDF DKVYDEFTMN GAVEKDEDAL RFVMEKSKAL GPGSYEEVLK RGFVGVGPSA 730 740 750 760 770 780 GKTGPVPADK PYRPFTVNVS EKVPYKTLTG RLQFYIDHDW YQRFGATVPK PQYGGGVLGP 790 800 810 820 830 840 KKYPFVYNTP HTRWGVHSFA RTDQWMLRHQ RGEPDVRLNP AAMARKGIKD GDQVRIFNSS 850 860 870 880 890 900 GEFFAMAKAW PGLPENMLFS EHGWEQYLYK NMTHYNSVNA ELINPLELVG GYGHVKFAAG 910 GFNPNRIFHE TTVDVEKA

References

[1]	"Cloning and sequencing of the genes encoding the periplasmic-cytochrome B-containing selenate reductase of Thauera selenatis." Krafft T., Bowen A., Theis F., Macy J.M. DNA Seq. 10:365-377(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Purification and characterization of the selenate reductase from Thauera selenatis." Schroeder I., Rech S., Krafft T., Macy J.M. J. Biol. Chem. 272:23765-23768(1997) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 37-51, CHARACTERIZATION.
[3]	"Crystallization and preliminary X-ray analysis of the selenate reductase from Thauera selenatis." Maher M.J., Macy J.M. Acta Crystallogr. D 58:706-708(2002) [PubMed] [Europe PMC] [Abstract] Cited for: CRYSTALLIZATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ007744 Genomic DNA. Translation: CAB53372.1.
3D structure databases
ProteinModelPortal	Q9S1H0.
ModBase	Search...
Protein family/group databases
TCDB	5.A.3.8.1. prokaryotic molybdopterin-containing oxidoreductase (PMO) family.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	2.40.40.20. 1 hit.
InterPro	IPR009010. Asp_de-COase-like_dom. IPR017840. DMSO_Rdtase_II_Mopterin_su. IPR006657. MoPterin_dinucl-bd_dom. IPR006656. Mopterin_OxRdtase. IPR006311. TAT_signal. [Graphical view]
Pfam	PF00384. Molybdopterin. 1 hit. PF01568. Molydop_binding. 1 hit. [Graphical view]
SUPFAM	SSF50692. Asp_decarb_fold. 1 hit.
TIGRFAMs	TIGR03479. DMSO_red_II_alp. 1 hit.
PROSITE	PS00551. MOLYBDOPTERIN_PROK_1. False negative. PS00490. MOLYBDOPTERIN_PROK_2. False negative. PS00932. MOLYBDOPTERIN_PROK_3. False negative. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SERA_THASE

Accession

Primary (citable) accession number: Q9S1H0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	May 1, 2000
Last modified:	May 1, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program