ID   DHE4_PSYT1              Reviewed;         448 AA.
AC   Q9S1F9;
DT   13-AUG-2002, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 4.
DT   16-JUN-2009, entry version 39.
DE   RecName: Full=NADP-specific glutamate dehydrogenase;
DE            Short=NADP-GDH;
DE            EC=1.4.1.4;
GN   Name=gdhA; Synonyms=gdh;
OS   Psychrobacter sp. (strain TAD1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Moraxellaceae; Psychrobacter.
OX   NCBI_TaxID=81861;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-448, AND
RP   CHARACTERIZATION.
RX   MEDLINE=20069353; PubMed=10601858;
RX   DOI=10.1046/j.1432-1327.2000.00972.x;
RA   Di Fraia R., Wilquet V., Ciardiello M.A., Carratore V., Antignani A.,
RA   Camardella L., Glansdorff N., Di Prisco G.;
RT   "NADP+-dependent glutamate dehydrogenase in the Antarctic
RT   psychrotolerant bacterium Psychrobacter sp. TAD1. Characterization,
RT   protein and DNA sequence, and relationship to other glutamate
RT   dehydrogenases.";
RL   Eur. J. Biochem. 267:121-131(2000).
CC   -!- CATALYTIC ACTIVITY: L-glutamate + H(2)O + NADP(+) = 2-oxoglutarate
CC       + NH(3) + NADPH.
CC   -!- SUBUNIT: Homohexamer.
CC   -!- SIMILARITY: Belongs to the Glu/Leu/Phe/Val dehydrogenases family.
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DR   EMBL; AJ010746; CAB54142.1; -; Genomic_DNA.
DR   HSSP; P24295; 1AUP.
DR   GO; GO:0005488; F:binding; IEA:InterPro.
DR   GO; GO:0004354; F:glutamate dehydrogenase (NADP+) activity; IEA:EC.
DR   GO; GO:0006520; P:amino acid metabolic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR006095; Glu/Leu/Phe/Val_DH.
DR   InterPro; IPR006096; Glu/Leu/Phe/Val_DH_C.
DR   InterPro; IPR006097; Glu/Leu/Phe/Val_DH_dimer.
DR   InterPro; IPR014362; Glu_DH.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   PANTHER; PTHR11606:SF2; GLFV_DH; 1.
DR   Pfam; PF00208; ELFV_dehydrog; 1.
DR   Pfam; PF02812; ELFV_dehydrog_N; 1.
DR   PIRSF; PIRSF000185; Glu_DH; 1.
DR   PRINTS; PR00082; GLFDHDRGNASE.
DR   PROSITE; PS00074; GLFV_DEHYDROGENASE; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; NADP; Oxidoreductase.
FT   INIT_MET      1      1       Removed.
FT   CHAIN         2    448       NADP-specific glutamate dehydrogenase.
FT                                /FTId=PRO_0000182774.
FT   ACT_SITE    124    124       By similarity.
SQ   SEQUENCE   448 AA;  49448 MW;  EE24FEA463DCB660 CRC64;
     MSISKAIEKV EARYAHQPEF IQAVKEVAIT IKPLYDAHPE YDKLKVFERL VEPDRVFGFR
     VNWEDDNGEI QINRGWRVQF SNALGPYKGG LRFHPTVNQS VLKFLGFEQI FKNALTGLPI
     GGGKGGSDFD PKGKTDSEIR RFCYAFMREL HHYVNKDMDV PAGDIGVGGR EVSYMFAMYK
     NLTRESTGVI TGKGVGFGGS LMRTEATGYG AVYFLQNMLA AQNESIEGKK VLVSGAGNVS
     LHAAEKATLI GAIVLTVSDS KGTIYDAKGL NQEKIDWLKV QKDQHKPLAD YVEVFGGEWM
     ADQKPWSIKA DIAIPSATQN EINEEDAKLL VDNGVKYIVE GANMPLTAEA IDYIRLHRVH
     YAPGKAANAG GVAVSALEMS QNSVRQYQTF EQVDERLQGI MKDIHDSSAQ ASEMYGQTDE
     GYIDYMSGAN MVGFKRVADA LVAFGILN
//