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Q9S1F9

- DHE4_PSYT1

UniProt

Q9S1F9 - DHE4_PSYT1

Protein

NADP-specific glutamate dehydrogenase

Gene

gdhA

Organism
Psychrobacter sp. (strain TAD1)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 57 (01 Oct 2014)
      Sequence version 4 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the reversible oxidative deamination of glutamate to alpha-ketoglutarate and ammonia.1 Publication

    Catalytic activityi

    L-glutamate + H2O + NADP+ = 2-oxoglutarate + NH3 + NADPH.

    Kineticsi

    1. KM=0.043 mM for NADP (at 20 degrees Celsius and at pH 8)1 Publication
    2. KM=4 mM for ammonium (at 20 degrees Celsius and at pH 8)1 Publication
    3. KM=67.4 mM for L-glutamate (at 20 degrees Celsius and at pH 8)1 Publication

    pH dependencei

    Optimum pH is 8.1 Publication

    Temperature dependencei

    Optimum temperature is 25 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei88 – 881SubstrateBy similarity
    Binding sitei109 – 1091SubstrateBy similarity
    Binding sitei112 – 1121SubstrateBy similarity
    Active sitei124 – 1241Proton donorPROSITE-ProRule annotation
    Binding sitei163 – 1631Substrate; via carbonyl oxygenBy similarity
    Sitei164 – 1641Important for catalysisBy similarity
    Binding sitei207 – 2071NADPBy similarity
    Binding sitei238 – 2381NADPBy similarity
    Binding sitei375 – 3751SubstrateBy similarity

    GO - Molecular functioni

    1. glutamate dehydrogenase (NADP+) activity Source: UniProtKB

    GO - Biological processi

    1. glutamate biosynthetic process Source: UniProtKB

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NADP

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADP-specific glutamate dehydrogenase (EC:1.4.1.4)
    Short name:
    NADP-GDH
    Gene namesi
    Name:gdhA
    Synonyms:gdh
    OrganismiPsychrobacter sp. (strain TAD1)
    Taxonomic identifieri81861 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaePsychrobacter

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 448447NADP-specific glutamate dehydrogenasePRO_0000182774Add
    BLAST

    Proteomic databases

    PRIDEiQ9S1F9.

    Interactioni

    Subunit structurei

    Homohexamer.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ9S1F9.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR006095. Glu/Leu/Phe/Val_DH.
    IPR006096. Glu/Leu/Phe/Val_DH_C.
    IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
    IPR014362. Glu_DH.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF00208. ELFV_dehydrog. 1 hit.
    PF02812. ELFV_dehydrog_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000185. Glu_DH. 1 hit.
    PRINTSiPR00082. GLFDHDRGNASE.
    SMARTiSM00839. ELFV_dehydrog. 1 hit.
    [Graphical view]
    PROSITEiPS00074. GLFV_DEHYDROGENASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9S1F9-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSISKAIEKV EARYAHQPEF IQAVKEVAIT IKPLYDAHPE YDKLKVFERL    50
    VEPDRVFGFR VNWEDDNGEI QINRGWRVQF SNALGPYKGG LRFHPTVNQS 100
    VLKFLGFEQI FKNALTGLPI GGGKGGSDFD PKGKTDSEIR RFCYAFMREL 150
    HHYVNKDMDV PAGDIGVGGR EVSYMFAMYK NLTRESTGVI TGKGVGFGGS 200
    LMRTEATGYG AVYFLQNMLA AQNESIEGKK VLVSGAGNVS LHAAEKATLI 250
    GAIVLTVSDS KGTIYDAKGL NQEKIDWLKV QKDQHKPLAD YVEVFGGEWM 300
    ADQKPWSIKA DIAIPSATQN EINEEDAKLL VDNGVKYIVE GANMPLTAEA 350
    IDYIRLHRVH YAPGKAANAG GVAVSALEMS QNSVRQYQTF EQVDERLQGI 400
    MKDIHDSSAQ ASEMYGQTDE GYIDYMSGAN MVGFKRVADA LVAFGILN 448
    Length:448
    Mass (Da):49,448
    Last modified:January 23, 2007 - v4
    Checksum:iEE24FEA463DCB660
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ010746 Genomic DNA. Translation: CAB54142.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ010746 Genomic DNA. Translation: CAB54142.1 .

    3D structure databases

    ProteinModelPortali Q9S1F9.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q9S1F9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR006095. Glu/Leu/Phe/Val_DH.
    IPR006096. Glu/Leu/Phe/Val_DH_C.
    IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
    IPR014362. Glu_DH.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    Pfami PF00208. ELFV_dehydrog. 1 hit.
    PF02812. ELFV_dehydrog_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000185. Glu_DH. 1 hit.
    PRINTSi PR00082. GLFDHDRGNASE.
    SMARTi SM00839. ELFV_dehydrog. 1 hit.
    [Graphical view ]
    PROSITEi PS00074. GLFV_DEHYDROGENASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "NADP+-dependent glutamate dehydrogenase in the Antarctic psychrotolerant bacterium Psychrobacter sp. TAD1. Characterization, protein and DNA sequence, and relationship to other glutamate dehydrogenases."
      Di Fraia R., Wilquet V., Ciardiello M.A., Carratore V., Antignani A., Camardella L., Glansdorff N., Di Prisco G.
      Eur. J. Biochem. 267:121-131(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-448, FUNCTION AS A GLUTAMATE DEHYDROGENASE, SUBUNIT, BIOPHYSICOCHEMICAL PROPERTIES.

    Entry informationi

    Entry nameiDHE4_PSYT1
    AccessioniPrimary (citable) accession number: Q9S1F9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 13, 2002
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 57 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3