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Protein

NADP-specific glutamate dehydrogenase

Gene

gdhA

Organism
Psychrobacter sp. (strain TAD1)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reversible oxidative deamination of glutamate to alpha-ketoglutarate and ammonia.1 Publication

Catalytic activityi

L-glutamate + H2O + NADP+ = 2-oxoglutarate + NH3 + NADPH.

Kineticsi

  1. KM=0.043 mM for NADP (at 20 degrees Celsius and at pH 8)1 Publication
  2. KM=4 mM for ammonium (at 20 degrees Celsius and at pH 8)1 Publication
  3. KM=67.4 mM for L-glutamate (at 20 degrees Celsius and at pH 8)1 Publication

    pH dependencei

    Optimum pH is 8.1 Publication

    Temperature dependencei

    Optimum temperature is 25 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei88SubstrateBy similarity1
    Binding sitei109SubstrateBy similarity1
    Binding sitei112SubstrateBy similarity1
    Active sitei124Proton donorPROSITE-ProRule annotation1
    Binding sitei163Substrate; via carbonyl oxygenBy similarity1
    Sitei164Important for catalysisBy similarity1
    Binding sitei207NADPBy similarity1
    Binding sitei238NADPBy similarity1
    Binding sitei375SubstrateBy similarity1

    GO - Molecular functioni

    • glutamate dehydrogenase (NADP+) activity Source: UniProtKB

    GO - Biological processi

    • cellular amino acid metabolic process Source: InterPro
    • glutamate biosynthetic process Source: UniProtKB
    Complete GO annotation...

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NADP

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADP-specific glutamate dehydrogenase (EC:1.4.1.4)
    Short name:
    NADP-GDH
    Gene namesi
    Name:gdhA
    Synonyms:gdh
    OrganismiPsychrobacter sp. (strain TAD1)
    Taxonomic identifieri81861 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaePsychrobacter

    Subcellular locationi

    GO - Cellular componenti

    Complete GO annotation...

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Initiator methionineiRemoved1 Publication
    ChainiPRO_00001827742 – 448NADP-specific glutamate dehydrogenaseAdd BLAST447

    Proteomic databases

    PRIDEiQ9S1F9.

    Interactioni

    Subunit structurei

    Homohexamer.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ9S1F9.
    SMRiQ9S1F9.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR006095. Glu/Leu/Phe/Val_DH.
    IPR033524. Glu/Leu/Phe/Val_DH_AS.
    IPR006096. Glu/Leu/Phe/Val_DH_C.
    IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
    IPR014362. Glu_DH.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF00208. ELFV_dehydrog. 1 hit.
    PF02812. ELFV_dehydrog_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000185. Glu_DH. 1 hit.
    PRINTSiPR00082. GLFDHDRGNASE.
    SMARTiSM00839. ELFV_dehydrog. 1 hit.
    [Graphical view]
    SUPFAMiSSF51735. SSF51735. 1 hit.
    PROSITEiPS00074. GLFV_DEHYDROGENASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9S1F9-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSISKAIEKV EARYAHQPEF IQAVKEVAIT IKPLYDAHPE YDKLKVFERL
    60 70 80 90 100
    VEPDRVFGFR VNWEDDNGEI QINRGWRVQF SNALGPYKGG LRFHPTVNQS
    110 120 130 140 150
    VLKFLGFEQI FKNALTGLPI GGGKGGSDFD PKGKTDSEIR RFCYAFMREL
    160 170 180 190 200
    HHYVNKDMDV PAGDIGVGGR EVSYMFAMYK NLTRESTGVI TGKGVGFGGS
    210 220 230 240 250
    LMRTEATGYG AVYFLQNMLA AQNESIEGKK VLVSGAGNVS LHAAEKATLI
    260 270 280 290 300
    GAIVLTVSDS KGTIYDAKGL NQEKIDWLKV QKDQHKPLAD YVEVFGGEWM
    310 320 330 340 350
    ADQKPWSIKA DIAIPSATQN EINEEDAKLL VDNGVKYIVE GANMPLTAEA
    360 370 380 390 400
    IDYIRLHRVH YAPGKAANAG GVAVSALEMS QNSVRQYQTF EQVDERLQGI
    410 420 430 440
    MKDIHDSSAQ ASEMYGQTDE GYIDYMSGAN MVGFKRVADA LVAFGILN
    Length:448
    Mass (Da):49,448
    Last modified:January 23, 2007 - v4
    Checksum:iEE24FEA463DCB660
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AJ010746 Genomic DNA. Translation: CAB54142.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AJ010746 Genomic DNA. Translation: CAB54142.1.

    3D structure databases

    ProteinModelPortaliQ9S1F9.
    SMRiQ9S1F9.
    ModBaseiSearch...
    MobiDBiSearch...

    Proteomic databases

    PRIDEiQ9S1F9.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR006095. Glu/Leu/Phe/Val_DH.
    IPR033524. Glu/Leu/Phe/Val_DH_AS.
    IPR006096. Glu/Leu/Phe/Val_DH_C.
    IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
    IPR014362. Glu_DH.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF00208. ELFV_dehydrog. 1 hit.
    PF02812. ELFV_dehydrog_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000185. Glu_DH. 1 hit.
    PRINTSiPR00082. GLFDHDRGNASE.
    SMARTiSM00839. ELFV_dehydrog. 1 hit.
    [Graphical view]
    SUPFAMiSSF51735. SSF51735. 1 hit.
    PROSITEiPS00074. GLFV_DEHYDROGENASE. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiDHE4_PSYT1
    AccessioniPrimary (citable) accession number: Q9S1F9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 13, 2002
    Last sequence update: January 23, 2007
    Last modified: November 2, 2016
    This is version 62 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.