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Q9S1F9

- DHE4_PSYT1

UniProt

Q9S1F9 - DHE4_PSYT1

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Protein

NADP-specific glutamate dehydrogenase

Gene
gdhA, gdh
Organism
Psychrobacter sp. (strain TAD1)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the reversible oxidative deamination of glutamate to alpha-ketoglutarate and ammonia.1 Publication

Catalytic activityi

L-glutamate + H2O + NADP+ = 2-oxoglutarate + NH3 + NADPH.

Kineticsi

  1. KM=0.043 mM for NADP (at 20 degrees Celsius and at pH 8)1 Publication
  2. KM=4 mM for ammonium (at 20 degrees Celsius and at pH 8)
  3. KM=67.4 mM for L-glutamate (at 20 degrees Celsius and at pH 8)

pH dependencei

Optimum pH is 8.

Temperature dependencei

Optimum temperature is 25 degrees Celsius.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei88 – 881Substrate By similarity
Binding sitei109 – 1091Substrate By similarity
Binding sitei112 – 1121Substrate By similarity
Active sitei124 – 1241Proton donor By similarity
Binding sitei163 – 1631Substrate; via carbonyl oxygen By similarity
Sitei164 – 1641Important for catalysis By similarity
Binding sitei207 – 2071NADP By similarity
Binding sitei238 – 2381NADP By similarity
Binding sitei375 – 3751Substrate By similarity

GO - Molecular functioni

  1. glutamate dehydrogenase (NADP+) activity Source: UniProtKB

GO - Biological processi

  1. glutamate biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Names & Taxonomyi

Protein namesi
Recommended name:
NADP-specific glutamate dehydrogenase (EC:1.4.1.4)
Short name:
NADP-GDH
Gene namesi
Name:gdhA
Synonyms:gdh
OrganismiPsychrobacter sp. (strain TAD1)
Taxonomic identifieri81861 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesMoraxellaceaePsychrobacter

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 448447NADP-specific glutamate dehydrogenasePRO_0000182774Add
BLAST

Proteomic databases

PRIDEiQ9S1F9.

Interactioni

Subunit structurei

Homohexamer.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ9S1F9.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR006095. Glu/Leu/Phe/Val_DH.
IPR006096. Glu/Leu/Phe/Val_DH_C.
IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
IPR014362. Glu_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00208. ELFV_dehydrog. 1 hit.
PF02812. ELFV_dehydrog_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000185. Glu_DH. 1 hit.
PRINTSiPR00082. GLFDHDRGNASE.
SMARTiSM00839. ELFV_dehydrog. 1 hit.
[Graphical view]
PROSITEiPS00074. GLFV_DEHYDROGENASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9S1F9-1 [UniParc]FASTAAdd to Basket

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MSISKAIEKV EARYAHQPEF IQAVKEVAIT IKPLYDAHPE YDKLKVFERL    50
VEPDRVFGFR VNWEDDNGEI QINRGWRVQF SNALGPYKGG LRFHPTVNQS 100
VLKFLGFEQI FKNALTGLPI GGGKGGSDFD PKGKTDSEIR RFCYAFMREL 150
HHYVNKDMDV PAGDIGVGGR EVSYMFAMYK NLTRESTGVI TGKGVGFGGS 200
LMRTEATGYG AVYFLQNMLA AQNESIEGKK VLVSGAGNVS LHAAEKATLI 250
GAIVLTVSDS KGTIYDAKGL NQEKIDWLKV QKDQHKPLAD YVEVFGGEWM 300
ADQKPWSIKA DIAIPSATQN EINEEDAKLL VDNGVKYIVE GANMPLTAEA 350
IDYIRLHRVH YAPGKAANAG GVAVSALEMS QNSVRQYQTF EQVDERLQGI 400
MKDIHDSSAQ ASEMYGQTDE GYIDYMSGAN MVGFKRVADA LVAFGILN 448
Length:448
Mass (Da):49,448
Last modified:January 23, 2007 - v4
Checksum:iEE24FEA463DCB660
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ010746 Genomic DNA. Translation: CAB54142.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ010746 Genomic DNA. Translation: CAB54142.1 .

3D structure databases

ProteinModelPortali Q9S1F9.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi Q9S1F9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
InterProi IPR006095. Glu/Leu/Phe/Val_DH.
IPR006096. Glu/Leu/Phe/Val_DH_C.
IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
IPR014362. Glu_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
Pfami PF00208. ELFV_dehydrog. 1 hit.
PF02812. ELFV_dehydrog_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF000185. Glu_DH. 1 hit.
PRINTSi PR00082. GLFDHDRGNASE.
SMARTi SM00839. ELFV_dehydrog. 1 hit.
[Graphical view ]
PROSITEi PS00074. GLFV_DEHYDROGENASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "NADP+-dependent glutamate dehydrogenase in the Antarctic psychrotolerant bacterium Psychrobacter sp. TAD1. Characterization, protein and DNA sequence, and relationship to other glutamate dehydrogenases."
    Di Fraia R., Wilquet V., Ciardiello M.A., Carratore V., Antignani A., Camardella L., Glansdorff N., Di Prisco G.
    Eur. J. Biochem. 267:121-131(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-448, FUNCTION AS A GLUTAMATE DEHYDROGENASE, SUBUNIT, BIOPHYSICOCHEMICAL PROPERTIES.

Entry informationi

Entry nameiDHE4_PSYT1
AccessioniPrimary (citable) accession number: Q9S1F9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 13, 2002
Last sequence update: January 23, 2007
Last modified: May 29, 2013
This is version 56 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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