Q9S1F9 (DHE4_PSYT1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADP-specific glutamate dehydrogenase
Short name=NADP-GDH
EC=1.4.1.4

Gene names

Name:	gdhA
Synonyms:	gdh

Organism

Psychrobacter sp. (strain TAD1)

Taxonomic identifier

81861 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Moraxellaceae › Psychrobacter

Protein attributes

Sequence length	448 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	L-glutamate + H2O + NADP+ = 2-oxoglutarate + NH3 + NADPH.
Subunit structure	Homohexamer.
Sequence similarities	Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	glutamate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 448	447	NADP-specific glutamate dehydrogenase		PRO_0000182774
Sites
	Active site	124	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9S1F9 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: EE24FEA463DCB660	FASTA	448	49,448
	10 20 30 40 50 60 MSISKAIEKV EARYAHQPEF IQAVKEVAIT IKPLYDAHPE YDKLKVFERL VEPDRVFGFR 70 80 90 100 110 120 VNWEDDNGEI QINRGWRVQF SNALGPYKGG LRFHPTVNQS VLKFLGFEQI FKNALTGLPI 130 140 150 160 170 180 GGGKGGSDFD PKGKTDSEIR RFCYAFMREL HHYVNKDMDV PAGDIGVGGR EVSYMFAMYK 190 200 210 220 230 240 NLTRESTGVI TGKGVGFGGS LMRTEATGYG AVYFLQNMLA AQNESIEGKK VLVSGAGNVS 250 260 270 280 290 300 LHAAEKATLI GAIVLTVSDS KGTIYDAKGL NQEKIDWLKV QKDQHKPLAD YVEVFGGEWM 310 320 330 340 350 360 ADQKPWSIKA DIAIPSATQN EINEEDAKLL VDNGVKYIVE GANMPLTAEA IDYIRLHRVH 370 380 390 400 410 420 YAPGKAANAG GVAVSALEMS QNSVRQYQTF EQVDERLQGI MKDIHDSSAQ ASEMYGQTDE 430 440 GYIDYMSGAN MVGFKRVADA LVAFGILN

References

[1]

"NADP+-dependent glutamate dehydrogenase in the Antarctic psychrotolerant bacterium Psychrobacter sp. TAD1. Characterization, protein and DNA sequence, and relationship to other glutamate dehydrogenases." Di Fraia R., Wilquet V., Ciardiello M.A., Carratore V., Antignani A., Camardella L., Glansdorff N., Di Prisco G. Eur. J. Biochem. 267:121-131(2000) [PubMed: 10601858] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-448, CHARACTERIZATION.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ010746 Genomic DNA. Translation: CAB54142.1.
3D structure databases
ProteinModelPortal	Q9S1F9.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR006095. Glu/Leu/Phe/Val_DH. IPR006096. Glu/Leu/Phe/Val_DH_C. IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom. IPR014362. Glu_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00208. ELFV_dehydrog. 1 hit. PF02812. ELFV_dehydrog_N. 1 hit. [Graphical view]
PIRSF	PIRSF000185. Glu_DH. 1 hit.
PRINTS	PR00082. GLFDHDRGNASE.
SMART	SM00839. ELFV_dehydrog. 1 hit. [Graphical view]
PROSITE	PS00074. GLFV_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHE4_PSYT1

Accession

Primary (citable) accession number: Q9S1F9

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 13, 2002
Last sequence update:	January 23, 2007
Last modified:	December 14, 2011
This is version 50 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program