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Q9S169 (BLA24_ECOLX) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-lactamase SHV-24

EC=3.5.2.6
Gene names
Name:bla
Synonyms:shv24
OrganismEscherichia coli
Taxonomic identifier562 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length286 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Hydrolyzes ampicillin. Can also hydrolyze cephaloridine, aztreonam and ceftazidime with a low catalytic rate. Ref.1

Catalytic activity

A beta-lactam + H2O = a substituted beta-amino acid.

Sequence similarities

Belongs to the class-A beta-lactamase family.

Biophysicochemical properties

Kinetic parameters:

KM=32 µM for ampicillin Ref.1

KM=210 µM for cephaloridine

KM=500 µM for aztreonam

KM=30 µM for ceftazidime

Vmax=0.366 µmol/min/µg enzyme with ampicillin as substrate

Vmax=0.434 µmol/min/µg enzyme with cephaloridine as substrate

Vmax=0.135 µmol/min/µg enzyme with aztreonam as substrate

Vmax=0.008 µmol/min/µg enzyme with ceftazidime as substrate

Ontologies

Keywords
   Biological processAntibiotic resistance
   DomainSignal
   Molecular functionHydrolase
   PTMDisulfide bond
Gene Ontology (GO)
   Biological_processbeta-lactam antibiotic catabolic process

Inferred from electronic annotation. Source: InterPro

response to antibiotic

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionbeta-lactamase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Potential
Chain22 – 286265Beta-lactamase SHV-24
PRO_0000016988

Regions

Region230 – 2323Substrate binding By similarity

Sites

Active site661Acyl-ester intermediate By similarity
Active site1641Proton acceptor By similarity

Amino acid modifications

Disulfide bond73 ↔ 119 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9S169 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 5EA9990BC8B0AAFF

FASTA28631,166
        10         20         30         40         50         60 
MRYIRLCIIS LLATLPLAVH ASPQPLEQIK LSESQLSGRV GMIEMDLASG RTLTAWRADE 

        70         80         90        100        110        120 
RFPMMSTFKV VLCGAVLARV DAGDEQLERK IHYRQQDLVD YSPVSEKHLA DGMTVGELCA 

       130        140        150        160        170        180 
AAITMSDNSA ANLLLATVGG PAGLTAFLRQ IGDNVTRLDR WETELNEALP GDARGTTTPA 

       190        200        210        220        230        240 
SMAATLRKLL TSQRLSARSQ RQLLQWMVDD RVAGPLIRSV LPAGWFIADK TGAGERGARG 

       250        260        270        280 
IVALLGPNNK AERIVVIYLR DTPASMAERN QQIAGIGAAL IEHWQR 

« Hide

References

[1]"A new SHV-derived extended-spectrum beta-lactamase (SHV-24) that hydrolyzes ceftazidime through a single-amino-acid substitution (D179G) in the omega-loop."
Kurokawa H., Yagi T., Shibata N., Shibayama K., Kamachi K., Arakawa Y.
Antimicrob. Agents Chemother. 44:1725-1727(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
Strain: HKY453.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB023477 Genomic DNA. Translation: BAA84973.1.

3D structure databases

ProteinModelPortalQ9S169.
SMRQ9S169. Positions 22-286.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

SABIO-RKQ9S169.

Family and domain databases

Gene3D3.40.710.10. 1 hit.
InterProIPR012338. Beta-lactam/transpept-like.
IPR000871. Beta-lactam_class-A/D.
IPR023650. Beta-lactam_class-A_AS.
[Graphical view]
PRINTSPR00118. BLACTAMASEA.
SUPFAMSSF56601. SSF56601. 1 hit.
PROSITEPS00146. BETA_LACTAMASE_A. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBLA24_ECOLX
AccessionPrimary (citable) accession number: Q9S169
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 2000
Last modified: October 16, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families