Q9S0X0 (Q9S0X0_CLOHI) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names	Submitted name: Class1 collagenase EMBL BAA86030.1
Organism	Clostridium histolyticum EMBL BAA86030.1
Taxonomic identifier	1498 [NCBI]
Taxonomic lineage	cellular organisms › Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	1118 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

Ontologies

Keywords
Domain	Collagen EMBL BAA86030.1
Ligand	Calcium PDB 1NQD PDB 2O8O Metal-binding PDB 1NQD PDB 2O8O
Technical term	3D-structure PDB 1NQD PDB 2O8O PDB 1NQJ
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular_component	collagen Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Metal binding	1009	1	Calcium 1 PDB 1NQD PDB 2O8O
	Metal binding	1011	1	Calcium 1 PDB 1NQD PDB 2O8O
	Metal binding	1011	1	Calcium 2 PDB 1NQD PDB 2O8O
	Metal binding	1013	1	Calcium 2 PDB 1NQD PDB 2O8O
	Metal binding	1014	1	Calcium 2 PDB 1NQD PDB 2O8O
	Metal binding	1032	1	Calcium 1; via carbonyl oxygen PDB 1NQD PDB 2O8O
	Metal binding	1037	1	Calcium 1 PDB 1NQD PDB 2O8O
	Metal binding	1037	1	Calcium 2 PDB 1NQD PDB 2O8O
	Metal binding	1039	1	Calcium 2; via carbonyl oxygen PDB 1NQD PDB 2O8O
	Metal binding	1040	1	Calcium 1 PDB 1NQD PDB 2O8O
	Metal binding	1040	1	Calcium 2 PDB 1NQD PDB 2O8O

Sequences

	Sequence		Length	Mass (Da)
	Q9S0X0 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 869F9E4B8DFB3313	FASTA	1,118	126,257
	10 20 30 40 50 60 MKKNILKILM DSYSKESKIQ TVRRVTSVSL LAVYLTMNTS SLVLAKPIEN TNDTSIKNVE 70 80 90 100 110 120 KLRNAPNEEN SKKVEDSKND KVEHVKNIEE AKVEQVAPEV KSKSTLRSAS IANTNSEKYD 130 140 150 160 170 180 FEYLNGLSYT ELTNLIKNIK WNQINGLFNY STGSQKFFGD KNRVQAIINA LQESGRTYTA 190 200 210 220 230 240 NDMKGIETFT EVLRAGFYLG YYNDGLSYLN DRNFQDKCIP AMIAIQKNPN FKLGTAVQDE 250 260 270 280 290 300 VITSLGKLIG NASANAEVVN NCVPVLKQFR ENLNQYAPDY VKGTAVNELI KGIEFDFSGA 310 320 330 340 350 360 AYEKDVKTMP WYGKIDPFIN ELKALGLYGN ITSATEWASD VGIYYLSKFG LYSTNRNDIV 370 380 390 400 410 420 QSLEKAVDMY KYGKIAFVAM ERITWDYDGI GSNGKKVDHD KFLDDAEKHY LPKTYTFDNG 430 440 450 460 470 480 TFIIRAGDKV SEEKIKRLYW ASREVKSQFH RVVGNDKALE VGNADDVLTM KIFNSPEEYK 490 500 510 520 530 540 FNTNINGVST DNGGLYIEPR GTFYTYERTP QQSIFSLEEL FRHEYTHYLQ ARYLVDGLWG 550 560 570 580 590 600 QGPFYEKNRL TWFDEGTAEF FAGSTRTSGV LPRKSILGYL AKDKVDHRYS LKKTLNSGYD 610 620 630 640 650 660 DSDWMVYNYG FAVAHYLYEK DMPTFIKMNK AILNTDVKSY DEIIKKLSDD ANKNTDYQTH 670 680 690 700 710 720 IQELADKYQG AGIPLVSDDY LKDHGYKKAS EVYSEISKAA SLTNTSVTAE KSQYFNTFTL 730 740 750 760 770 780 RGTYTGETSK GEFKDWDEMS KKLDGTLESL AKNSWSGYKT LTAYFTNYRV TSDNKVQYDV 790 800 810 820 830 840 VFHGVLTDNA DISNNKAPIA KVTGPSTGAV GRNIEFSGKD SKDEDGKIVS YDWDFVYGAT 850 860 870 880 890 900 SRGKNSVHAY KKAGTYNVTL KVTDDKGATA TESFTIEIKN EDTTTPITKE MEPNDDIKEA 910 920 930 940 950 960 NGPIVEGVTV KGDLNGSDDA DTFYFDVKED GDVTIELPYS GSSNFTWLVY KEGDDQNHIA 970 980 990 1000 1010 1020 SGIDKNNSKV GTFKSTKGRH YVFIYKHDSA SNISYSLNIK GLGNEKLKEK ENNDSSDKAT 1030 1040 1050 1060 1070 1080 VIPNFNTTMQ GSLLGDDSRD YYSFEVKEEG EVNIELDKKD EFGVTWTLHP ESNINDRITY 1090 1100 1110 GQVDGNKVSN KVKLRPGKYY LLVYKYSGSG NYELRVNK

References

[1]	"Class2 collagenase." Hosaka T., Yamato I. Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: JCM1403 EMBL BAA86030.1.
[2]	"A bacterial collagen-binding domain with novel calcium-binding motif controls domain orientation." Wilson J.J., Matsushita O., Okabe A., Sakon J. EMBO J. 22:1743-1752(2003) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.00 ANGSTROMS) OF 1003-1118 IN COMPLEX WITH CALCIUM.
[3]	"Induction of stable beta-sheet by Ca2+ in Clostridial collagen binding domain." Philominathan S.T.L., Wilson J.J., Matsushita O., Sakon J. Submitted (DEC-2006) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (1.35 ANGSTROMS) OF 1003-1118 IN COMPLEX WITH CALCIUM.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AB026889 Genomic DNA. Translation: BAA86030.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1NQD	X-ray	1.65	A/B	1003-1118	[»]
1NQJ	X-ray	1.00	A/B	1003-1118	[»]
2O8O	X-ray	1.35	A/B	1003-1118	[»]

ProteinModelPortal

Q9S0X0.

SMR

Q9S0X0. Positions 1005-1118.

ModBase

Search...

Protein family/group databases

Allergome

5989. Clo hi Collagenase.

MEROPS

M09.002.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

2.60.40.670. 1 hit.

InterPro

IPR007280. Peptidase_C_arc/bac.
IPR013661. Peptidase_M9_N_dom.
IPR002169. Peptidase_M9A/M9B.
IPR022409. PKD/Chitinase_dom.
IPR000601. PKD_dom.
[Graphical view]

Pfam

PF01752. Peptidase_M9. 1 hit.
PF08453. Peptidase_M9_N. 1 hit.
PF00801. PKD. 1 hit.
PF04151. PPC. 1 hit.
[Graphical view]

PRINTS

PR00931. MICOLLPTASE.

SMART

SM00089. PKD. 1 hit.
[Graphical view]

SUPFAM

SSF49299. PKD. 1 hit.

PROSITE

PS50093. PKD. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

BindingDB

Q9S0X0.

EvolutionaryTrace

Q9S0X0.

Entry information

Entry name

Q9S0X0_CLOHI

Accession

Primary (citable) accession number: Q9S0X0

Entry history

Integrated into UniProtKB/TrEMBL:	May 1, 2000
Last sequence update:	May 1, 2000
Last modified:	April 3, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)