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Protein

NH(3)-dependent NAD(+) synthetase

Gene

nadE

Organism
Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+.UniRule annotation

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes NAD(+) from deamido-NAD(+) (ammonia route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. NH(3)-dependent NAD(+) synthetase (nadE)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes NAD(+) from deamido-NAD(+) (ammonia route), the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei55UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi53 – 60ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, NAD, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00253; UER00333.

Names & Taxonomyi

Protein namesi
Recommended name:
NH(3)-dependent NAD(+) synthetaseUniRule annotation (EC:6.3.1.5UniRule annotation)
Gene namesi
Name:nadEUniRule annotation
Ordered Locus Names:DR_A0201
OrganismiDeinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
Taxonomic identifieri243230 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciDeinococcalesDeinococcaceaeDeinococcus
Proteomesi
  • UP000002524 Componenti: Chromosome II

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001521661 – 287NH(3)-dependent NAD(+) synthetaseAdd BLAST287

Interactioni

Protein-protein interaction databases

STRINGi243230.DR_A0201.

Structurei

Secondary structure

1287
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi10 – 19Combined sources10
Helixi27 – 43Combined sources17
Beta strandi49 – 53Combined sources5
Helixi58 – 76Combined sources19
Beta strandi82 – 87Combined sources6
Beta strandi90 – 92Combined sources3
Helixi97 – 106Combined sources10
Beta strandi109 – 113Combined sources5
Helixi117 – 131Combined sources15
Helixi137 – 159Combined sources23
Beta strandi162 – 164Combined sources3
Helixi169 – 174Combined sources6
Turni179 – 183Combined sources5
Turni189 – 192Combined sources4
Helixi195 – 204Combined sources10
Helixi209 – 211Combined sources3
Helixi229 – 233Combined sources5
Helixi237 – 244Combined sources8
Helixi251 – 263Combined sources13
Helixi264 – 267Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4Q16X-ray2.60A/B/C/D1-287[»]
ProteinModelPortaliQ9RYV5.
SMRiQ9RYV5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C4K. Bacteria.
COG0171. LUCA.
HOGENOMiHOG000238070.
InParanoidiQ9RYV5.
KOiK01916.
OMAiAKIIEGW.
OrthoDBiPOG091H02ZG.

Family and domain databases

CDDicd00553. NAD_synthase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00193. NadE. 1 hit.
InterProiIPR022310. NAD/GMP_synthase.
IPR003694. NAD_synthase.
IPR022926. NH(3)-dep_NAD(+)_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF02540. NAD_synthase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00552. nadE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9RYV5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTPSPLPLSP LRSHIIRELH VQPDIDPGAE VERRVAFLCD YLQSTPTKGF
60 70 80 90 100
VLGISGGQDS TLAGRLCQLA VERRRSQGHG ATFLAVRLPY GVQADEADAQ
110 120 130 140 150
QALDFIQADR EVTVNIKEAA DASVAAAQAA LGSEVRDFVR GNVKARERMV
160 170 180 190 200
AQYALAGQEN LLVVGTDHAA EALTGFYTKY GDGGVDLTPL SGLTKRQGAQ
210 220 230 240 250
LLAHLGAPEG TWRKVPTADL EDDRPGLPDE VALGVTYAQI DAYLEGREVS
260 270 280
DEAAARLERL FLNSRHKRAL PVTPFDGWWQ PGEQKQS
Length:287
Mass (Da):31,077
Last modified:May 1, 2000 - v1
Checksum:i3258CD1549967F69
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001825 Genomic DNA. Translation: AAF12179.1.
PIRiA75617.
RefSeqiNP_285524.1. NC_001264.1.
WP_010889460.1. NZ_CP015082.1.

Genome annotation databases

EnsemblBacteriaiAAF12179; AAF12179; DR_A0201.
GeneIDi1797961.
KEGGidra:DR_A0201.
PATRICi21633442. VBIDeiRad64572_3090.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE001825 Genomic DNA. Translation: AAF12179.1.
PIRiA75617.
RefSeqiNP_285524.1. NC_001264.1.
WP_010889460.1. NZ_CP015082.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4Q16X-ray2.60A/B/C/D1-287[»]
ProteinModelPortaliQ9RYV5.
SMRiQ9RYV5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243230.DR_A0201.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAF12179; AAF12179; DR_A0201.
GeneIDi1797961.
KEGGidra:DR_A0201.
PATRICi21633442. VBIDeiRad64572_3090.

Phylogenomic databases

eggNOGiENOG4105C4K. Bacteria.
COG0171. LUCA.
HOGENOMiHOG000238070.
InParanoidiQ9RYV5.
KOiK01916.
OMAiAKIIEGW.
OrthoDBiPOG091H02ZG.

Enzyme and pathway databases

UniPathwayiUPA00253; UER00333.

Family and domain databases

CDDicd00553. NAD_synthase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00193. NadE. 1 hit.
InterProiIPR022310. NAD/GMP_synthase.
IPR003694. NAD_synthase.
IPR022926. NH(3)-dep_NAD(+)_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF02540. NAD_synthase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00552. nadE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNADE_DEIRA
AccessioniPrimary (citable) accession number: Q9RYV5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: May 1, 2000
Last modified: November 2, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.