Q9RYV5 (NADE_DEIRA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase
EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	DR_A0201

Organism

Deinococcus radiodurans

Taxonomic identifier

1299 [NCBI]

Taxonomic lineage

Bacteria › Deinococcus-Thermus › Deinococci › Deinococcales › Deinococcaceae › Deinococcus

Protein attributes

Sequence length	287 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 287	287	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_0000152166
Regions
	Nucleotide binding	53 – 60	8	ATP By similarity
Sites
	Active site	55	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9RYV5 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 3258CD1549967F69	FASTA	287	31,077
	10 20 30 40 50 60 MTPSPLPLSP LRSHIIRELH VQPDIDPGAE VERRVAFLCD YLQSTPTKGF VLGISGGQDS 70 80 90 100 110 120 TLAGRLCQLA VERRRSQGHG ATFLAVRLPY GVQADEADAQ QALDFIQADR EVTVNIKEAA 130 140 150 160 170 180 DASVAAAQAA LGSEVRDFVR GNVKARERMV AQYALAGQEN LLVVGTDHAA EALTGFYTKY 190 200 210 220 230 240 GDGGVDLTPL SGLTKRQGAQ LLAHLGAPEG TWRKVPTADL EDDRPGLPDE VALGVTYAQI 250 260 270 280 DAYLEGREVS DEAAARLERL FLNSRHKRAL PVTPFDGWWQ PGEQKQS

References

[1]

"Genome sequence of the radioresistant bacterium Deinococcus radiodurans R1." White O., Eisen J.A., Heidelberg J.F., Hickey E.K., Peterson J.D., Dodson R.J., Haft D.H., Gwinn M.L., Nelson W.C., Richardson D.L., Moffat K.S., Qin H., Jiang L., Pamphile W., Crosby M., Shen M., Vamathevan J.J., Lam P. , McDonald L.A., Utterback T.R., Zalewski C., Makarova K.S., Aravind L., Daly M.J., Minton K.W., Fleischmann R.D., Ketchum K.A., Nelson K.E., Salzberg S.L., Smith H.O., Venter J.C., Fraser C.M. Science 286:1571-1577(1999) [PubMed: 10567266] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE001825 Genomic DNA. Translation: AAF12179.1.
PIR	A75617.
RefSeq	NP_285524.1. NC_001264.1.
3D structure databases
ProteinModelPortal	Q9RYV5.
SMR	Q9RYV5. Positions 11-280.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1797961.
GenomeReviews	Gene locus DR_A0201 in contig AE001825_GR.
KEGG	dra:DR_A0201.
NMPDR	fig|243230.1.peg.3014.
PATRIC	21633442. VBIDeiRad64572_3090.
TIGR	DR_A0201.
Phylogenomic databases
HOGENOM	HBG351567.
OMA	IAQYEIA.
PhylomeDB	Q9RYV5.
ProtClustDB	PRK00768.
Enzyme and pathway databases
BioCyc	DRAD243230:DR_A0201-MONOMER.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_DEIRA

Accession

Primary (citable) accession number: Q9RYV5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 15, 2002
Last sequence update:	May 1, 2000
Last modified:	January 25, 2012
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program