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Protein

N5-carboxyaminoimidazole ribonucleotide synthase

Gene

purK

Organism
Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the ATP-dependent conversion of 5-aminoimidazole ribonucleotide (AIR) and HCO3- to N5-carboxyaminoimidazole ribonucleotide (N5-CAIR).UniRule annotation
Catalyzes the ATP-dependent conversion of 5-aminoimidazole ribonucleotide (AIR) and HCO(3)- to N5-carboxyaminoimidazole ribonucleotide (N5-CAIR).UniRule annotation

Catalytic activityi

ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole + HCO3- = ADP + phosphate + 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole.UniRule annotation

Pathway:iIMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (N5-CAIR route).UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. N5-carboxyaminoimidazole ribonucleotide synthase (purK)
  2. N5-carboxyaminoimidazole ribonucleotide mutase (purE)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (N5-CAIR route), the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei103 – 1031ATPUniRule annotation
Binding sitei143 – 1431ATPUniRule annotation
Binding sitei186 – 1861ATPUniRule annotation
Binding sitei209 – 2091ATPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi148 – 1547ATPUniRule annotation
Nucleotide bindingi178 – 1814ATPUniRule annotation
Nucleotide bindingi263 – 2642ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotation

Keywords - Biological processi

Purine biosynthesisUniRule annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciDRAD243230:GH46-26-MONOMER.
UniPathwayiUPA00074; UER00942.

Names & Taxonomyi

Protein namesi
Recommended name:
N5-carboxyaminoimidazole ribonucleotide synthaseUniRule annotation (EC:6.3.4.18UniRule annotation)
Short name:
N5-CAIR synthaseUniRule annotation
Alternative name(s):
5-(carboxyamino)imidazole ribonucleotide synthetaseUniRule annotation
Gene namesi
Name:purKUniRule annotation
Ordered Locus Names:DR_0024Imported
OrganismiDeinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)Imported
Taxonomic identifieri243230 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciDeinococcalesDeinococcaceaeDeinococcus
ProteomesiUP000002524 Componenti: Chromosome I

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi243230.DR_0024.

Structurei

3D structure databases

ProteinModelPortaliQ9RYC6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini107 – 293187ATP-graspUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the PurK/PurT family.UniRule annotation
Contains 1 ATP-grasp domain.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000034026.
InParanoidiQ9RYC6.
KOiK01589.
OMAiLRKMGHV.
OrthoDBiEOG6QZMX7.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPiMF_01928. PurK.
InterProiIPR011761. ATP-grasp.
IPR003135. ATP-grasp_carboxylate-amine.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR005875. PurK.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamiPF02222. ATP-grasp. 1 hit.
[Graphical view]
SUPFAMiSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01161. purK. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9RYC6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDQPAATLG ILGGGQLAQM LALAALPLGV QVRVLEPDAE APARLCAEHV
60 70 80 90 100
HAPYTDPAGL DALAACDAVT LEFENVPVES LDALRGRVPV RPGAGLLARS
110 120 130 140 150
KHRAREKAAL QEAGARTAPF VVIQREADLD GALDRVGGQG ILKTSELGYD
160 170 180 190 200
GKGQVRVASD TDLRQGWAEL GQVACILEGF VTFEREVSLA VARTADGRLA
210 220 230 240 250
FGPLVENVHR DGILRTSVYP AAVPDGTEAR AREMARAVAE GWGLEGLLTL
260 270 280 290 300
EFFQVPDGDL LVNEVAPRVH NSGHLTQDGG GASQFEAQVR AVLGLPLSDW
310 320 330 340 350
SPLLPCAMVN YVGVEGAAPD WAAIDALPGT RVHLYHKAHR TGRKLGHVNL
360 370
AAPDLPTLHQ RLAQLEQLIP
Length:370
Mass (Da):39,175
Last modified:May 1, 2000 - v1
Checksum:i56E4564CB3033B38
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000513 Genomic DNA. Translation: AAF09616.1.
PIRiA75569.
RefSeqiNP_293750.1. NC_001263.1.
WP_010886672.1. NC_001263.1.

Genome annotation databases

EnsemblBacteriaiAAF09616; AAF09616; DR_0024.
GeneIDi1797266.
KEGGidra:DR_0024.
PATRICi21627519. VBIDeiRad64572_0189.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000513 Genomic DNA. Translation: AAF09616.1.
PIRiA75569.
RefSeqiNP_293750.1. NC_001263.1.
WP_010886672.1. NC_001263.1.

3D structure databases

ProteinModelPortaliQ9RYC6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243230.DR_0024.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAF09616; AAF09616; DR_0024.
GeneIDi1797266.
KEGGidra:DR_0024.
PATRICi21627519. VBIDeiRad64572_0189.

Phylogenomic databases

HOGENOMiHOG000034026.
InParanoidiQ9RYC6.
KOiK01589.
OMAiLRKMGHV.
OrthoDBiEOG6QZMX7.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00942.
BioCyciDRAD243230:GH46-26-MONOMER.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPiMF_01928. PurK.
InterProiIPR011761. ATP-grasp.
IPR003135. ATP-grasp_carboxylate-amine.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR005875. PurK.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamiPF02222. ATP-grasp. 1 hit.
[Graphical view]
SUPFAMiSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01161. purK. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422Imported.

Entry informationi

Entry nameiQ9RYC6_DEIRA
AccessioniPrimary (citable) accession number: Q9RYC6
Entry historyi
Integrated into UniProtKB/TrEMBL: May 1, 2000
Last sequence update: May 1, 2000
Last modified: July 22, 2015
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.