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Protein

Malate dehydrogenase

Gene

mdh

Organism
Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible oxidation of malate to oxaloacetate.UniRule annotation

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei94 – 941SubstrateUniRule annotation
Binding sitei100 – 1001SubstrateUniRule annotation
Binding sitei107 – 1071NADUniRule annotation
Binding sitei114 – 1141NADUniRule annotation
Binding sitei133 – 1331SubstrateUniRule annotation
Binding sitei164 – 1641SubstrateUniRule annotation
Active sitei189 – 1891Proton acceptorUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi13 – 197NADUniRule annotation
Nucleotide bindingi131 – 1333NADUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciDRAD243230:GH46-335-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenaseUniRule annotation (EC:1.1.1.37UniRule annotation)
Gene namesi
Name:mdhUniRule annotation
Ordered Locus Names:DR_0325
OrganismiDeinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
Taxonomic identifieri243230 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciDeinococcalesDeinococcaceaeDeinococcus
ProteomesiUP000002524 Componenti: Chromosome I

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 330330Malate dehydrogenasePRO_0000113368Add
BLAST

Proteomic databases

PRIDEiQ9RXI8.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi243230.DR_0325.

Structurei

3D structure databases

ProteinModelPortaliQ9RXI8.
SMRiQ9RXI8. Positions 3-329.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 2 family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0039.
HOGENOMiHOG000220953.
InParanoidiQ9RXI8.
KOiK00024.
OMAiFVTTVQQ.
OrthoDBiEOG6PP9Q2.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_01517. Malate_dehydrog_2.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23382. PTHR23382. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9RXI8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTMKQPVRVA VTGAAGQIGY SLLFRIAAGD MLGQDQPVIL QLLEITPALK
60 70 80 90 100
ALNGVVMELR DGAFPLLADV ITSDDPMVAF KDADYALLVG AMPRKAGMER
110 120 130 140 150
GDLLGANGGI FKPQGEALGA VASRNVKVLV VGNPANTNAL IAQQNAPDLD
160 170 180 190 200
PKCFTAMVRL DHNRALSQLA EKTGAAVSDI KNVTIWGNHS STQYPDLSQA
210 220 230 240 250
TVNGKPALEQ VDRTWYENDY IPTVAKRGAA IIEARGASSA ASAASAAIDH
260 270 280 290 300
MHDWALGTKD GEWVSMGIPS DGSYGIPEGL IYGFPVRVKD GKYEIVQGLD
310 320 330
VSDFSRGKMD ATAQELEEER DEVRKLGLVK
Length:330
Mass (Da):35,148
Last modified:May 1, 2000 - v1
Checksum:i2F9710386D3A2110
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000513 Genomic DNA. Translation: AAF09906.1.
PIRiE75535.
RefSeqiNP_294048.1. NC_001263.1.
WP_010886970.1. NC_001263.1.

Genome annotation databases

EnsemblBacteriaiAAF09906; AAF09906; DR_0325.
GeneIDi1798897.
KEGGidra:DR_0325.
PATRICi21628148. VBIDeiRad64572_0491.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000513 Genomic DNA. Translation: AAF09906.1.
PIRiE75535.
RefSeqiNP_294048.1. NC_001263.1.
WP_010886970.1. NC_001263.1.

3D structure databases

ProteinModelPortaliQ9RXI8.
SMRiQ9RXI8. Positions 3-329.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243230.DR_0325.

Proteomic databases

PRIDEiQ9RXI8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAF09906; AAF09906; DR_0325.
GeneIDi1798897.
KEGGidra:DR_0325.
PATRICi21628148. VBIDeiRad64572_0491.

Phylogenomic databases

eggNOGiCOG0039.
HOGENOMiHOG000220953.
InParanoidiQ9RXI8.
KOiK00024.
OMAiFVTTVQQ.
OrthoDBiEOG6PP9Q2.

Enzyme and pathway databases

BioCyciDRAD243230:GH46-335-MONOMER.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_01517. Malate_dehydrog_2.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23382. PTHR23382. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422.

Entry informationi

Entry nameiMDH_DEIRA
AccessioniPrimary (citable) accession number: Q9RXI8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: May 1, 2000
Last modified: May 27, 2015
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.