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Protein

Malto-oligosyltrehalose trehalohydrolase

Gene

treZ

Organism
Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of (1->4)-alpha-D-glucosidic linkage in 4-alpha-D-((1->4)-alpha-D-glucanosyl)(n) trehalose to yield trehalose and (1->4)-alpha-D-glucan.

Pathwayi: trehalose biosynthesis

This protein is involved in the pathway trehalose biosynthesis, which is part of Glycan biosynthesis.
View all proteins of this organism that are known to be involved in the pathway trehalose biosynthesis and in Glycan biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei275 – 2751NucleophileCurated
Active sitei308 – 3081Proton donorCurated
Binding sitei376 – 3761Substrate1 Publication
Sitei400 – 4001Transition state stabilizerCurated

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism

Enzyme and pathway databases

BioCyciDRAD243230:GH46-475-MONOMER.
BRENDAi3.2.1.141. 1856.
UniPathwayiUPA00299.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Malto-oligosyltrehalose trehalohydrolase (EC:3.2.1.141)
Short name:
MTHase
Alternative name(s):
4-alpha-D-((1->4)-alpha-D-glucano)trehalose trehalohydrolase
Maltooligosyl trehalose trehalohydrolase
Gene namesi
Name:treZ
Ordered Locus Names:DR_0464
OrganismiDeinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
Taxonomic identifieri243230 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciDeinococcalesDeinococcaceaeDeinococcus
Proteomesi
  • UP000002524 Componenti: Chromosome I

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 600600Malto-oligosyltrehalose trehalohydrolasePRO_0000393750Add
BLAST

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

STRINGi243230.DR_0464.

Structurei

Secondary structure

1
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi21 – 233Combined sources
Beta strandi25 – 295Combined sources
Helixi31 – 333Combined sources
Beta strandi36 – 416Combined sources
Beta strandi46 – 527Combined sources
Beta strandi55 – 584Combined sources
Beta strandi60 – 634Combined sources
Beta strandi66 – 727Combined sources
Beta strandi78 – 836Combined sources
Beta strandi86 – 883Combined sources
Helixi109 – 1113Combined sources
Helixi124 – 1263Combined sources
Beta strandi129 – 1324Combined sources
Helixi134 – 1374Combined sources
Beta strandi138 – 1403Combined sources
Helixi143 – 1486Combined sources
Helixi150 – 1567Combined sources
Beta strandi160 – 1634Combined sources
Beta strandi170 – 1723Combined sources
Beta strandi181 – 1855Combined sources
Helixi187 – 1893Combined sources
Helixi192 – 20413Combined sources
Beta strandi208 – 2136Combined sources
Beta strandi220 – 2223Combined sources
Helixi225 – 2284Combined sources
Helixi230 – 2323Combined sources
Beta strandi233 – 2386Combined sources
Beta strandi240 – 2456Combined sources
Helixi250 – 26718Combined sources
Beta strandi270 – 2745Combined sources
Helixi277 – 2793Combined sources
Beta strandi284 – 2863Combined sources
Helixi288 – 29710Combined sources
Beta strandi304 – 3085Combined sources
Helixi315 – 3184Combined sources
Beta strandi323 – 3264Combined sources
Helixi329 – 33911Combined sources
Helixi344 – 3485Combined sources
Helixi353 – 36210Combined sources
Beta strandi363 – 3653Combined sources
Beta strandi367 – 3715Combined sources
Beta strandi378 – 3814Combined sources
Helixi389 – 3913Combined sources
Beta strandi392 – 3965Combined sources
Helixi399 – 4035Combined sources
Helixi411 – 4133Combined sources
Helixi419 – 43012Combined sources
Beta strandi431 – 4377Combined sources
Helixi440 – 4423Combined sources
Helixi456 – 46914Combined sources
Turni470 – 4723Combined sources
Helixi474 – 4774Combined sources
Helixi487 – 4915Combined sources
Helixi498 – 5014Combined sources
Helixi503 – 52119Combined sources
Turni523 – 5264Combined sources
Helixi530 – 5323Combined sources
Beta strandi533 – 5386Combined sources
Beta strandi541 – 5488Combined sources
Beta strandi551 – 5588Combined sources
Beta strandi560 – 5623Combined sources
Helixi566 – 5683Combined sources
Beta strandi578 – 5825Combined sources
Beta strandi587 – 5915Combined sources
Beta strandi596 – 6005Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2BHUX-ray1.10A1-600[»]
2BHYX-ray1.50A1-600[»]
2BHZX-ray1.20A1-600[»]
2BXYX-ray1.75A1-600[»]
2BXZX-ray1.75A1-600[»]
2BY0X-ray1.55A1-600[»]
2BY1X-ray1.55A1-600[»]
2BY2X-ray1.50A1-600[»]
2BY3X-ray1.50A1-600[»]
ProteinModelPortaliQ9RX51.
SMRiQ9RX51. Positions 14-600.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9RX51.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni273 – 2786Substrate bindingBy similarity
Regioni328 – 3325Substrate binding1 Publication
Regioni399 – 4046Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Phylogenomic databases

eggNOGiENOG4105C9C. Bacteria.
COG0296. LUCA.
HOGENOMiHOG000155669.
InParanoidiQ9RX51.
KOiK01236.
OMAiFTPMLFM.
OrthoDBiPOG091H0CAL.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR022567. DUF3459.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR012768. Trehalose_TreZ.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 3 hits.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02922. CBM_48. 1 hit.
PF11941. DUF3459. 1 hit.
[Graphical view]
PIRSFiPIRSF006337. Trehalose_TreZ. 1 hit.
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsiTIGR02402. trehalose_TreZ. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9RX51-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQTQPVTPT PPASFQTQHD PRTRLGATPL PGGAGTRFRL WTSTARTVAV
60 70 80 90 100
RVNGTEHVMT SLGGGIYELE LPVGPGARYL FVLDGVPTPD PYARFLPDGV
110 120 130 140 150
HGEAEVVDFG TFDWTDADWH GIKLADCVFY EVHVGTFTPE GTYRAAAEKL
160 170 180 190 200
PYLKELGVTA IQVMPLAAFD GQRGWGYDGA AFYAPYAPYG RPEDLMALVD
210 220 230 240 250
AAHRLGLGVF LDVVYNHFGP SGNYLSSYAP SYFTDRFSSA WGMGLDYAEP
260 270 280 290 300
HMRRYVTGNA RMWLRDYHFD GLRLDATPYM TDDSETHILT ELAQEIHELG
310 320 330 340 350
GTHLLLAEDH RNLPDLVTVN HLDGIWTDDF HHETRVTLTG EQEGYYAGYR
360 370 380 390 400
GGAEALAYTI RRGWRYEGQF WAVKGEEHER GHPSDALEAP NFVYCIQNHD
410 420 430 440 450
QIGNRPLGER LHQSDGVTLH EYRGAAALLL PMTPLLFQGQ EWAASTPFQF
460 470 480 490 500
FSDHAGELGQ AVSEGRKKEF GGFSGFSGED VPDPQAEQTF LNSKLNWAER
510 520 530 540 550
EGGEHARTLR LYRDLLRLRR EDPVLHNRQR ENLTTGHDGD VLWVRTVTGA
560 570 580 590 600
GERVLLWNLG QDTRAVAEVK LPFTVPRRLL LHTEGREDLT LGAGEAVLVG
Length:600
Mass (Da):66,910
Last modified:May 1, 2000 - v1
Checksum:i594091EC093F8A44
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000513 Genomic DNA. Translation: AAF10042.1.
PIRiH75516.
RefSeqiNP_294187.1. NC_001263.1.
WP_010887109.1. NC_001263.1.

Genome annotation databases

EnsemblBacteriaiAAF10042; AAF10042; DR_0464.
GeneIDi1798275.
KEGGidra:DR_0464.
PATRICi21628450. VBIDeiRad64572_0641.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000513 Genomic DNA. Translation: AAF10042.1.
PIRiH75516.
RefSeqiNP_294187.1. NC_001263.1.
WP_010887109.1. NC_001263.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2BHUX-ray1.10A1-600[»]
2BHYX-ray1.50A1-600[»]
2BHZX-ray1.20A1-600[»]
2BXYX-ray1.75A1-600[»]
2BXZX-ray1.75A1-600[»]
2BY0X-ray1.55A1-600[»]
2BY1X-ray1.55A1-600[»]
2BY2X-ray1.50A1-600[»]
2BY3X-ray1.50A1-600[»]
ProteinModelPortaliQ9RX51.
SMRiQ9RX51. Positions 14-600.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243230.DR_0464.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAF10042; AAF10042; DR_0464.
GeneIDi1798275.
KEGGidra:DR_0464.
PATRICi21628450. VBIDeiRad64572_0641.

Phylogenomic databases

eggNOGiENOG4105C9C. Bacteria.
COG0296. LUCA.
HOGENOMiHOG000155669.
InParanoidiQ9RX51.
KOiK01236.
OMAiFTPMLFM.
OrthoDBiPOG091H0CAL.

Enzyme and pathway databases

UniPathwayiUPA00299.
BioCyciDRAD243230:GH46-475-MONOMER.
BRENDAi3.2.1.141. 1856.

Miscellaneous databases

EvolutionaryTraceiQ9RX51.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR022567. DUF3459.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR012768. Trehalose_TreZ.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 3 hits.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02922. CBM_48. 1 hit.
PF11941. DUF3459. 1 hit.
[Graphical view]
PIRSFiPIRSF006337. Trehalose_TreZ. 1 hit.
SMARTiSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsiTIGR02402. trehalose_TreZ. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTREZ_DEIRA
AccessioniPrimary (citable) accession number: Q9RX51
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 20, 2010
Last sequence update: May 1, 2000
Last modified: September 7, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.