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Q9RWN1

- PFKA_DEIRA

UniProt

Q9RWN1 - PFKA_DEIRA

Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 2 (27 Apr 2001)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

    Catalytic activityi

    ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Enzyme regulationi

    Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei19 – 191ATP; via amide nitrogenUniRule annotation
    Metal bindingi111 – 1111Magnesium; catalyticUniRule annotation
    Active sitei136 – 1361Proton acceptorUniRule annotation
    Binding sitei163 – 1631Allosteric activator ADPUniRule annotation
    Binding sitei171 – 1711Substrate; shared with dimeric partnerUniRule annotation
    Binding sitei220 – 2201Allosteric activator ADPUniRule annotation
    Binding sitei231 – 2311SubstrateUniRule annotation
    Binding sitei253 – 2531Substrate; shared with dimeric partnerUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi80 – 812ATPUniRule annotation
    Nucleotide bindingi110 – 1134ATPUniRule annotation

    GO - Molecular functioni

    1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
    2. ATP binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. fructose 6-phosphate metabolic process Source: InterPro
    2. glycolytic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciDRAD243230:GH46-654-MONOMER.
    UniPathwayiUPA00109; UER00182.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
    Short name:
    ATP-PFKUniRule annotation
    Short name:
    PhosphofructokinaseUniRule annotation
    Alternative name(s):
    PhosphohexokinaseUniRule annotation
    Gene namesi
    Name:pfkAUniRule annotation
    Ordered Locus Names:DR_0635
    OrganismiDeinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
    Taxonomic identifieri243230 [NCBI]
    Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciDeinococcalesDeinococcaceaeDeinococcus
    ProteomesiUP000002524: Chromosome I

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. 6-phosphofructokinase complex Source: InterPro

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 329329ATP-dependent 6-phosphofructokinasePRO_0000111949Add
    BLAST

    Proteomic databases

    PRIDEiQ9RWN1.

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Protein-protein interaction databases

    STRINGi243230.DR_0635.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9RWN1.
    SMRiQ9RWN1. Positions 9-329.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni29 – 335Allosteric activator ADP binding; shared with dimeric partnerUniRule annotation
    Regioni134 – 1363Substrate bindingUniRule annotation
    Regioni178 – 1803Substrate bindingUniRule annotation
    Regioni194 – 1963Allosteric activator ADP bindingUniRule annotation
    Regioni222 – 2243Allosteric activator ADP bindingUniRule annotation
    Regioni259 – 2624Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0205.
    HOGENOMiHOG000248870.
    KOiK00850.
    OMAiGIYRGYK.
    OrthoDBiEOG644ZRM.

    Family and domain databases

    HAMAPiMF_00339. Phosphofructokinase_I_B1.
    InterProiIPR012003. ATP_PFK_prok.
    IPR012828. PFKA_ATP.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view]
    PfamiPF00365. PFK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSiPR00476. PHFRCTKINASE.
    SUPFAMiSSF53784. SSF53784. 1 hit.
    TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
    PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9RWN1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAYPNPAGIK RVAVLTSGGD APGMNAAIRA VVRTGAQQGI EVVGVRRGFS    50
    GLHRGEVQLL GPRDVANTIQ RGGTILLTAR SHTWRSPEGR AKGAAVLREW 100
    GVDGLVVIGG DGSFHGGYFL QEEHGIPVIG VPGTIDNDLY GTDHTIGYFT 150
    AVETALEAVD KLRDTGASHE RIFVIEVMGR HAGHIALDVA VAGGAEEVFI 200
    PEDKKPIGDV VNIVKDSIAK GKRGSIIIVA EGYDGGAEAV AQAIHAGTGL 250
    ETRVSILGHI QRGGTPVSSD RILASRLGEA AVYALMDGKK GVMVGRIGGG 300
    IAYTPLHETW EKRKDVSRDL YRCAKMLSI 329
    Length:329
    Mass (Da):34,621
    Last modified:April 27, 2001 - v2
    Checksum:i2EF8BE617C593238
    GO

    Sequence cautioni

    The sequence AAF10213.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000513 Genomic DNA. Translation: AAF10213.1. Different initiation.
    PIRiA75495.
    RefSeqiNP_294358.1. NC_001263.1.

    Genome annotation databases

    EnsemblBacteriaiAAF10213; AAF10213; DR_0635.
    GeneIDi1799138.
    KEGGidra:DR_0635.
    PATRICi21628812. VBIDeiRad64572_0814.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000513 Genomic DNA. Translation: AAF10213.1 . Different initiation.
    PIRi A75495.
    RefSeqi NP_294358.1. NC_001263.1.

    3D structure databases

    ProteinModelPortali Q9RWN1.
    SMRi Q9RWN1. Positions 9-329.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243230.DR_0635.

    Proteomic databases

    PRIDEi Q9RWN1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAF10213 ; AAF10213 ; DR_0635 .
    GeneIDi 1799138.
    KEGGi dra:DR_0635.
    PATRICi 21628812. VBIDeiRad64572_0814.

    Phylogenomic databases

    eggNOGi COG0205.
    HOGENOMi HOG000248870.
    KOi K00850.
    OMAi GIYRGYK.
    OrthoDBi EOG644ZRM.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00182 .
    BioCyci DRAD243230:GH46-654-MONOMER.

    Family and domain databases

    HAMAPi MF_00339. Phosphofructokinase_I_B1.
    InterProi IPR012003. ATP_PFK_prok.
    IPR012828. PFKA_ATP.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view ]
    Pfami PF00365. PFK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSi PR00476. PHFRCTKINASE.
    SUPFAMi SSF53784. SSF53784. 1 hit.
    TIGRFAMsi TIGR02482. PFKA_ATP. 1 hit.
    PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422.

    Entry informationi

    Entry nameiPFKA_DEIRA
    AccessioniPrimary (citable) accession number: Q9RWN1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 27, 2001
    Last sequence update: April 27, 2001
    Last modified: October 1, 2014
    This is version 101 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3