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Q9RWN1 (PFKA_DEIRA) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ATP-dependent 6-phosphofructokinase

Short name=ATP-PFK
Short name=Phosphofructokinase
EC=2.7.1.11
Alternative name(s):
Phosphohexokinase
Gene names
Name:pfkA
Ordered Locus Names:DR_0635
OrganismDeinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422) [Reference proteome] [HAMAP]
Taxonomic identifier243230 [NCBI]
Taxonomic lineageBacteriaDeinococcus-ThermusDeinococciDeinococcalesDeinococcaceaeDeinococcus

Protein attributes

Sequence length329 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity. HAMAP-Rule MF_00339

Catalytic activity

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. HAMAP-Rule MF_00339

Cofactor

Magnesium By similarity. HAMAP-Rule MF_00339

Enzyme regulation

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate By similarity. HAMAP-Rule MF_00339

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. HAMAP-Rule MF_00339

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_00339

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00339.

Sequence similarities

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.

Sequence caution

The sequence AAF10213.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 329329ATP-dependent 6-phosphofructokinase HAMAP-Rule MF_00339
PRO_0000111949

Regions

Nucleotide binding80 – 812ATP By similarity
Nucleotide binding110 – 1134ATP By similarity
Region29 – 335Allosteric activator ADP binding; shared with dimeric partner By similarity
Region134 – 1363Substrate binding By similarity
Region178 – 1803Substrate binding By similarity
Region194 – 1963Allosteric activator ADP binding By similarity
Region222 – 2243Allosteric activator ADP binding By similarity
Region259 – 2624Substrate binding By similarity

Sites

Active site1361Proton acceptor By similarity
Metal binding1111Magnesium; catalytic By similarity
Binding site191ATP; via amide nitrogen By similarity
Binding site1631Allosteric activator ADP By similarity
Binding site1711Substrate; shared with dimeric partner By similarity
Binding site2201Allosteric activator ADP By similarity
Binding site2311Substrate By similarity
Binding site2531Substrate; shared with dimeric partner By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9RWN1 [UniParc].

Last modified April 27, 2001. Version 2.
Checksum: 2EF8BE617C593238

FASTA32934,621
        10         20         30         40         50         60 
MAYPNPAGIK RVAVLTSGGD APGMNAAIRA VVRTGAQQGI EVVGVRRGFS GLHRGEVQLL 

        70         80         90        100        110        120 
GPRDVANTIQ RGGTILLTAR SHTWRSPEGR AKGAAVLREW GVDGLVVIGG DGSFHGGYFL 

       130        140        150        160        170        180 
QEEHGIPVIG VPGTIDNDLY GTDHTIGYFT AVETALEAVD KLRDTGASHE RIFVIEVMGR 

       190        200        210        220        230        240 
HAGHIALDVA VAGGAEEVFI PEDKKPIGDV VNIVKDSIAK GKRGSIIIVA EGYDGGAEAV 

       250        260        270        280        290        300 
AQAIHAGTGL ETRVSILGHI QRGGTPVSSD RILASRLGEA AVYALMDGKK GVMVGRIGGG 

       310        320 
IAYTPLHETW EKRKDVSRDL YRCAKMLSI 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE000513 Genomic DNA. Translation: AAF10213.1. Different initiation.
PIRA75495.
RefSeqNP_294358.1. NC_001263.1.

3D structure databases

ProteinModelPortalQ9RWN1.
SMRQ9RWN1. Positions 9-329.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243230.DR_0635.

Proteomic databases

PRIDEQ9RWN1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAF10213; AAF10213; DR_0635.
GeneID1799138.
KEGGdra:DR_0635.
PATRIC21628812. VBIDeiRad64572_0814.

Phylogenomic databases

eggNOGCOG0205.
HOGENOMHOG000248870.
KOK00850.
OMAGIYRGYK.
OrthoDBEOG644ZRM.

Enzyme and pathway databases

BioCycDRAD243230:GH46-654-MONOMER.
UniPathwayUPA00109; UER00182.

Family and domain databases

HAMAPMF_00339. Phosphofructokinase_I_B1.
InterProIPR012003. ATP_PFK_prok.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamPF00365. PFK. 1 hit.
[Graphical view]
PIRSFPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSPR00476. PHFRCTKINASE.
SUPFAMSSF53784. SSF53784. 1 hit.
TIGRFAMsTIGR02482. PFKA_ATP. 1 hit.
PROSITEPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePFKA_DEIRA
AccessionPrimary (citable) accession number: Q9RWN1
Entry history
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: April 27, 2001
Last modified: July 9, 2014
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways