Q9RWN1 (K6PF_DEIRA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
6-phosphofructokinase
Short name=Phosphofructokinase
EC=2.7.1.11

Alternative name(s):
Phosphohexokinase

Gene names

Name:	pfkA
Ordered Locus Names:	DR_0635

Organism

Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422) [Reference proteome] [HAMAP]

Taxonomic identifier

243230 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Deinococcus-Thermus › Deinococci › Deinococcales › Deinococcaceae › Deinococcus › Deinococcus radiodurans

Protein attributes

Sequence length	329 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. HAMAP-Rule MF_00339
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 3/4. HAMAP-Rule MF_00339
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the phosphofructokinase family.
Sequence caution	The sequence AAF10213.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Allosteric enzyme Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	fructose 6-phosphate metabolic process Inferred from electronic annotation. Source: InterPro glycolysis Inferred from electronic annotation. Source: HAMAP
Cellular_component	6-phosphofructokinase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	6-phosphofructokinase activity Inferred from electronic annotation. Source: HAMAP ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 329	329	6-phosphofructokinase HAMAP-Rule MF_00339		PRO_0000111949
Regions
	Nucleotide binding	29 – 33	5	ATP By similarity
	Nucleotide binding	163 – 167	5	ATP By similarity
	Nucleotide binding	180 – 196	17	ATP By similarity
Sites
	Active site	136	1	Proton acceptor By similarity
	Metal binding	194	1	Magnesium; via carbonyl oxygen By similarity
	Metal binding	196	1	Magnesium By similarity
	Binding site	171	1	Substrate By similarity
	Binding site	253	1	Substrate By similarity
	Binding site	259	1	Substrate By similarity
	Binding site	262	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9RWN1 [UniParc]. Last modified April 27, 2001. Version 2. Checksum: 2EF8BE617C593238	FASTA	329	34,621
	10 20 30 40 50 60 MAYPNPAGIK RVAVLTSGGD APGMNAAIRA VVRTGAQQGI EVVGVRRGFS GLHRGEVQLL 70 80 90 100 110 120 GPRDVANTIQ RGGTILLTAR SHTWRSPEGR AKGAAVLREW GVDGLVVIGG DGSFHGGYFL 130 140 150 160 170 180 QEEHGIPVIG VPGTIDNDLY GTDHTIGYFT AVETALEAVD KLRDTGASHE RIFVIEVMGR 190 200 210 220 230 240 HAGHIALDVA VAGGAEEVFI PEDKKPIGDV VNIVKDSIAK GKRGSIIIVA EGYDGGAEAV 250 260 270 280 290 300 AQAIHAGTGL ETRVSILGHI QRGGTPVSSD RILASRLGEA AVYALMDGKK GVMVGRIGGG 310 320 IAYTPLHETW EKRKDVSRDL YRCAKMLSI

References

[1]

"Genome sequence of the radioresistant bacterium Deinococcus radiodurans R1." White O., Eisen J.A., Heidelberg J.F., Hickey E.K., Peterson J.D., Dodson R.J., Haft D.H., Gwinn M.L., Nelson W.C., Richardson D.L., Moffat K.S., Qin H., Jiang L., Pamphile W., Crosby M., Shen M., Vamathevan J.J., Lam P. , McDonald L.A., Utterback T.R., Zalewski C., Makarova K.S., Aravind L., Daly M.J., Minton K.W., Fleischmann R.D., Ketchum K.A., Nelson K.E., Salzberg S.L., Smith H.O., Venter J.C., Fraser C.M. Science 286:1571-1577(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000513 Genomic DNA. Translation: AAF10213.1. Different initiation.
PIR	A75495.
RefSeq	NP_294358.1. NC_001263.1.
3D structure databases
ProteinModelPortal	Q9RWN1.
SMR	Q9RWN1. Positions 9-329.
ModBase	Search...
Protein-protein interaction databases
STRING	243230.DR_0635.
Proteomic databases
PRIDE	Q9RWN1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAF10213; AAF10213; DR_0635.
GeneID	1799138.
KEGG	dra:DR_0635.
PATRIC	21628812. VBIDeiRad64572_0814.
Phylogenomic databases
eggNOG	COG0205.
HOGENOM	HOG000248870.
KO	K00850.
OMA	ARIFAQE.
ProtClustDB	PRK03202.
Enzyme and pathway databases
BioCyc	DRAD243230:GH46-1006-MONOMER.
UniPathway	UPA00109; UER00182.
Family and domain databases
HAMAP	MF_00339. Phosphofructokinase.
InterPro	IPR012003. ATP_PFK_prok. IPR012828. PFKA_ATP. IPR022953. Phosphofructokinase. IPR015912. Phosphofructokinase_CS. IPR000023. Phosphofructokinase_dom. [Graphical view]
Pfam	PF00365. PFK. 1 hit. [Graphical view]
PIRSF	PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTS	PR00476. PHFRCTKINASE.
SUPFAM	SSF53784. Ppfruckinase. 1 hit.
TIGRFAMs	TIGR02482. PFKA_ATP. 1 hit.
PROSITE	PS00433. PHOSPHOFRUCTOKINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

K6PF_DEIRA

Accession

Primary (citable) accession number: Q9RWN1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	April 27, 2001
Last modified:	May 1, 2013
This is version 92 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program