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Q9RWN1

- PFKA_DEIRA

UniProt

Q9RWN1 - PFKA_DEIRA

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Protein
ATP-dependent 6-phosphofructokinase
Gene
pfkA, DR_0635
Organism
Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis By similarity.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Enzyme regulationi

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei19 – 191ATP; via amide nitrogen By similarity
Metal bindingi111 – 1111Magnesium; catalytic By similarity
Active sitei136 – 1361Proton acceptor By similarity
Binding sitei163 – 1631Allosteric activator ADP By similarity
Binding sitei171 – 1711Substrate; shared with dimeric partner By similarity
Binding sitei220 – 2201Allosteric activator ADP By similarity
Binding sitei231 – 2311Substrate By similarity
Binding sitei253 – 2531Substrate; shared with dimeric partner By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi80 – 812ATP By similarity
Nucleotide bindingi110 – 1134ATP By similarity

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciDRAD243230:GH46-654-MONOMER.
UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinase (EC:2.7.1.11)
Short name:
ATP-PFK
Short name:
Phosphofructokinase
Alternative name(s):
Phosphohexokinase
Gene namesi
Name:pfkA
Ordered Locus Names:DR_0635
OrganismiDeinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
Taxonomic identifieri243230 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciDeinococcalesDeinococcaceaeDeinococcus
ProteomesiUP000002524: Chromosome I

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. 6-phosphofructokinase complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 329329ATP-dependent 6-phosphofructokinaseUniRule annotation
PRO_0000111949Add
BLAST

Proteomic databases

PRIDEiQ9RWN1.

Interactioni

Subunit structurei

Homotetramer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi243230.DR_0635.

Structurei

3D structure databases

ProteinModelPortaliQ9RWN1.
SMRiQ9RWN1. Positions 9-329.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni29 – 335Allosteric activator ADP binding; shared with dimeric partner By similarity
Regioni134 – 1363Substrate binding By similarity
Regioni178 – 1803Substrate binding By similarity
Regioni194 – 1963Allosteric activator ADP binding By similarity
Regioni222 – 2243Allosteric activator ADP binding By similarity
Regioni259 – 2624Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0205.
HOGENOMiHOG000248870.
KOiK00850.
OMAiGIYRGYK.
OrthoDBiEOG644ZRM.

Family and domain databases

HAMAPiMF_00339. Phosphofructokinase_I_B1.
InterProiIPR012003. ATP_PFK_prok.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9RWN1-1 [UniParc]FASTAAdd to Basket

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MAYPNPAGIK RVAVLTSGGD APGMNAAIRA VVRTGAQQGI EVVGVRRGFS    50
GLHRGEVQLL GPRDVANTIQ RGGTILLTAR SHTWRSPEGR AKGAAVLREW 100
GVDGLVVIGG DGSFHGGYFL QEEHGIPVIG VPGTIDNDLY GTDHTIGYFT 150
AVETALEAVD KLRDTGASHE RIFVIEVMGR HAGHIALDVA VAGGAEEVFI 200
PEDKKPIGDV VNIVKDSIAK GKRGSIIIVA EGYDGGAEAV AQAIHAGTGL 250
ETRVSILGHI QRGGTPVSSD RILASRLGEA AVYALMDGKK GVMVGRIGGG 300
IAYTPLHETW EKRKDVSRDL YRCAKMLSI 329
Length:329
Mass (Da):34,621
Last modified:April 27, 2001 - v2
Checksum:i2EF8BE617C593238
GO

Sequence cautioni

The sequence AAF10213.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000513 Genomic DNA. Translation: AAF10213.1. Different initiation.
PIRiA75495.
RefSeqiNP_294358.1. NC_001263.1.

Genome annotation databases

EnsemblBacteriaiAAF10213; AAF10213; DR_0635.
GeneIDi1799138.
KEGGidra:DR_0635.
PATRICi21628812. VBIDeiRad64572_0814.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000513 Genomic DNA. Translation: AAF10213.1 . Different initiation.
PIRi A75495.
RefSeqi NP_294358.1. NC_001263.1.

3D structure databases

ProteinModelPortali Q9RWN1.
SMRi Q9RWN1. Positions 9-329.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 243230.DR_0635.

Proteomic databases

PRIDEi Q9RWN1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAF10213 ; AAF10213 ; DR_0635 .
GeneIDi 1799138.
KEGGi dra:DR_0635.
PATRICi 21628812. VBIDeiRad64572_0814.

Phylogenomic databases

eggNOGi COG0205.
HOGENOMi HOG000248870.
KOi K00850.
OMAi GIYRGYK.
OrthoDBi EOG644ZRM.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00182 .
BioCyci DRAD243230:GH46-654-MONOMER.

Family and domain databases

HAMAPi MF_00339. Phosphofructokinase_I_B1.
InterProi IPR012003. ATP_PFK_prok.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view ]
Pfami PF00365. PFK. 1 hit.
[Graphical view ]
PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSi PR00476. PHFRCTKINASE.
SUPFAMi SSF53784. SSF53784. 1 hit.
TIGRFAMsi TIGR02482. PFKA_ATP. 1 hit.
PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422.

Entry informationi

Entry nameiPFKA_DEIRA
AccessioniPrimary (citable) accession number: Q9RWN1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: April 27, 2001
Last modified: July 9, 2014
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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