Reviewed, UniProtKB/Swiss-Prot Q9RWI4 (CARA_DEIRA)

Names and origin

Protein names

Recommended name:
    Carbamoyl-phosphate synthase small chain
    EC=6.3.5.5
Alternative name(s):
    Carbamoyl-phosphate synthetase glutamine chain

Gene names

Name:	carA
Ordered Locus Names:	DR_0684

Organism

Deinococcus radiodurans [Complete proteome] [HAMAP]

Taxonomic identifier

1299 [NCBI]

Taxonomic lineage

Bacteria › Deinococcus-Thermus › Deinococci › Deinococcales › Deinococcaceae › Deinococcus

Protein attributes

Sequence length	394 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate. HAMAP MF_01209
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from HCO(3)(-): step 1/1. HAMAP MF_01209 Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 1/6. HAMAP MF_01209
Subunit structure	Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate By similarity.
Sequence similarities	Belongs to the carA family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis Pyrimidine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 394	394	Carbamoyl-phosphate synthase small chain HAMAP MF_01209		PRO_0000112272
Regions
	Domain	192 – 379	188	Glutamine amidotransferase type-1
	Region	1 – 188	188	CPSase HAMAP MF_01209
Sites
	Active site	267	1	Nucleophile By similarity
	Active site	352	1	By similarity
	Active site	354	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9RWI4-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 479F5F896ECA1161	FASTA	394	43,518
	10 20 30 40 50 60 MIRKERAILA LEDGTVYRGY AFGYRGETVG EVVFNTSMTG YQEIMTDPSY NGQIVTITYP 70 80 90 100 110 120 HVGNYGVAIY DMESNKPYVR GFISREFSGE YSNYRAQQSL EAFMQQYGVV SIQGIDTRAL 130 140 150 160 170 180 VRRLRSGGVV KGVIAHRSFT HPEDPYGEFT PAEEQIYVQR ARDHQDIDGF DMTKEVTTAL 190 200 210 220 230 240 PYAFPTLRQG KRVVLMDFGI KHTIIERLAE VGIEPIVVPA HTTPAQIMAL QPHGLFLSNG 250 260 270 280 290 300 PGDPAPLEYA HKTAWEMMGL LPTFGICLGH QILGLAAGGQ TFKMKFGHRG GNQPVKNLLT 310 320 330 340 350 360 GNVEITSQNH GYAVDLDSIP NGAFVATHVN LNDGTLEGMA HSRYPVFSVQ YHPEASPGPH 370 380 390 DSRYLFDRFI EEIDAFDGGN GTPIIKANPG RLGV

References

[1]

"Genome sequence of the radioresistant bacterium Deinococcus radiodurans R1." White O., Eisen J.A., Heidelberg J.F., Hickey E.K., Peterson J.D., Dodson R.J., Haft D.H., Gwinn M.L., Nelson W.C., Richardson D.L., Moffat K.S., Qin H., Jiang L., Pamphile W., Crosby M., Shen M., Vamathevan J.J., Lam P. , McDonald L.A., Utterback T.R., Zalewski C., Makarova K.S., Aravind L., Daly M.J., Minton K.W., Fleischmann R.D., Ketchum K.A., Nelson K.E., Salzberg S.L., Smith H.O., Venter J.C., Fraser C.M. Science 286:1571-1577(1999) [PubMed: 10567266] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13939 / DSM 20539 / IFO 15346 / LMG 4051 / NCIB 9279 / R1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000513 Genomic DNA. Translation: AAF10261.1.
PIR	A75489.
RefSeq	NP_294407.1.
3D structure databases
HSSP	HSSP built from PDB template 1A9X based on UniProtKB P00907.
ModBase	Search...
Genome annotation databases
GeneID	1799232.
GenomeReviews	Gene locus DR_0684 in contig AE000513_GR.
KEGG	dra:DR_0684.
NMPDR	fig|243230.1.peg.866.
TIGR	DR_0684.
Phylogenomic databases
HOGENOM	Q9RWI4.
OMA	Q9RWI4. LFDGSNC.
Enzyme and pathway databases
BioCyc	DRAD243230:DR_0684-MON.
BRENDA	6.3.5.5. 96172.
Family and domain databases
HAMAP	MF_01209. [Tree]
InterPro	IPR006220. Anth_synthII. IPR001317. CarbamoylP_synth_GATase. IPR006274. CarbamoylP_synth_ssu. IPR002474. CarbamoylP_synth_ssu_N. IPR011702. GATASE. IPR017926. GATASE_1. IPR000991. GATase_class1_C. [Graphical view]
PANTHER	PTHR11405:SF4. CarA_synth_small. 1 hit.
Pfam	PF00988. CPSase_sm_chain. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
PRINTS	PR00097. ANTSNTHASEII. PR00099. CPSGATASE. PR00096. GATASE.
TIGRFAMs	TIGR01368. CPSaseIIsmall. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CARA_DEIRA

Accession

Primary (citable) accession number: Q9RWI4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2002
Last sequence update:	May 1, 2000
Last modified:	June 16, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)