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Protein

Citrate synthase

Gene

gltA

Organism
Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Might regulate the synthesis and function of enzymes involved in later enzymatic steps of Krebs cycle. Loss in activity results in sporulation defect (By similarity).By similarity

Catalytic activityi

Acetyl-CoA + H2O + oxaloacetate = citrate + CoA.PROSITE-ProRule annotationBy similarity

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes isocitrate from oxaloacetate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Citrate synthase (gltA)
  2. Aconitate hydratase A (acn)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isocitrate from oxaloacetate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei220PROSITE-ProRule annotation1
Active sitei259PROSITE-ProRule annotation1
Active sitei313PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Tricarboxylic acid cycle

Enzyme and pathway databases

UniPathwayiUPA00223; UER00717.

Names & Taxonomyi

Protein namesi
Recommended name:
Citrate synthase (EC:2.3.3.16)
Gene namesi
Name:gltA
Ordered Locus Names:DR_0757
OrganismiDeinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
Taxonomic identifieri243230 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciDeinococcalesDeinococcaceaeDeinococcus
Proteomesi
  • UP000002524 Componenti: Chromosome I

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001699422 – 377Citrate synthaseAdd BLAST376

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi243230.DR_0757.

Structurei

3D structure databases

ProteinModelPortaliQ9RWB2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the citrate synthase family.UniRule annotationCurated

Phylogenomic databases

eggNOGiENOG4105BZN. Bacteria.
COG0372. LUCA.
HOGENOMiHOG000021225.
InParanoidiQ9RWB2.
KOiK01647.
OMAiEDPRATH.
OrthoDBiPOG091H00T5.

Family and domain databases

Gene3Di1.10.230.10. 1 hit.
1.10.580.10. 1 hit.
InterProiIPR011278. 2-MeCitrate/Citrate_synth_II.
IPR016142. Citrate_synth-like_lrg_a-sub.
IPR016143. Citrate_synth-like_sm_a-sub.
IPR002020. Citrate_synthase.
IPR019810. Citrate_synthase_AS.
IPR024176. Citrate_synthase_bac-typ.
[Graphical view]
PANTHERiPTHR11739. PTHR11739. 1 hit.
PfamiPF00285. Citrate_synt. 1 hit.
[Graphical view]
PIRSFiPIRSF001369. Citrate_synth. 1 hit.
PRINTSiPR00143. CITRTSNTHASE.
SUPFAMiSSF48256. SSF48256. 1 hit.
TIGRFAMsiTIGR01800. cit_synth_II. 1 hit.
PROSITEiPS00480. CITRATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9RWB2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNIAKGLEG VLFTESKLTF INGSEGILTH LGIPIQEWAE KSTFEELSLA
60 70 80 90 100
LLDAKLPTAE ELAKFDAELK ANRAIPDQLV GIIRDMPKGV HPMQALRTAV
110 120 130 140 150
SYLGLLDPQA EDITPEARRA ISTRMIAQFS TIIAAINRAQ EGQDIVAPRA
160 170 180 190 200
DLTHAGNFLY MLTGNEPTPE QARLFDIALV LHADHGMNAS TFTAIATSST
210 220 230 240 250
LSDMYSCMVS AIGALKGPLH GGANEAVMTM LDEIGTVDKA EAYITGKLDN
260 270 280 290 300
KEKIMGVGHR VYKYFDPRSR VLRDYAEHVA NKEGKSNYYQ ILEAIEKIIV
310 320 330 340 350
DRMGAKGIYP NVDFYSGTVY SDLGIKKEYF TPIFALARIS GWCASVIEYS
360 370
QDNRLLRPDA EYTGARDQHY VDIKDRQ
Length:377
Mass (Da):41,628
Last modified:January 23, 2007 - v3
Checksum:i268F74D5B8EC37DA
GO

Sequence cautioni

The sequence AAF10336 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000513 Genomic DNA. Translation: AAF10336.1. Different initiation.
PIRiA75479.
RefSeqiNP_294481.1. NC_001263.1.
WP_027479989.1. NZ_CP015081.1.

Genome annotation databases

EnsemblBacteriaiAAF10336; AAF10336; DR_0757.
GeneIDi1798731.
KEGGidra:DR_0757.
PATRICi21629062. VBIDeiRad64572_0937.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000513 Genomic DNA. Translation: AAF10336.1. Different initiation.
PIRiA75479.
RefSeqiNP_294481.1. NC_001263.1.
WP_027479989.1. NZ_CP015081.1.

3D structure databases

ProteinModelPortaliQ9RWB2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243230.DR_0757.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAF10336; AAF10336; DR_0757.
GeneIDi1798731.
KEGGidra:DR_0757.
PATRICi21629062. VBIDeiRad64572_0937.

Phylogenomic databases

eggNOGiENOG4105BZN. Bacteria.
COG0372. LUCA.
HOGENOMiHOG000021225.
InParanoidiQ9RWB2.
KOiK01647.
OMAiEDPRATH.
OrthoDBiPOG091H00T5.

Enzyme and pathway databases

UniPathwayiUPA00223; UER00717.

Family and domain databases

Gene3Di1.10.230.10. 1 hit.
1.10.580.10. 1 hit.
InterProiIPR011278. 2-MeCitrate/Citrate_synth_II.
IPR016142. Citrate_synth-like_lrg_a-sub.
IPR016143. Citrate_synth-like_sm_a-sub.
IPR002020. Citrate_synthase.
IPR019810. Citrate_synthase_AS.
IPR024176. Citrate_synthase_bac-typ.
[Graphical view]
PANTHERiPTHR11739. PTHR11739. 1 hit.
PfamiPF00285. Citrate_synt. 1 hit.
[Graphical view]
PIRSFiPIRSF001369. Citrate_synth. 1 hit.
PRINTSiPR00143. CITRTSNTHASE.
SUPFAMiSSF48256. SSF48256. 1 hit.
TIGRFAMsiTIGR01800. cit_synth_II. 1 hit.
PROSITEiPS00480. CITRATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCISY_DEIRA
AccessioniPrimary (citable) accession number: Q9RWB2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 108 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Citrate synthase is found in nearly all cells capable of oxidative metabolism.By similarity

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.