Q9RUZ0 (CITEL_DEIRA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Citrate lyase subunit beta-like protein
EC=4.1.-.-

Gene names

Ordered Locus Names:

DR_1240

Organism

Deinococcus radiodurans

Taxonomic identifier

1299 [NCBI]

Taxonomic lineage

Bacteria › Deinococcus-Thermus › Deinococci › Deinococcales › Deinococcaceae › Deinococcus

Protein attributes

Sequence length	284 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play a role in fatty acid biosynthesis Potential.
Cofactor	Binds 1 magnesium ion per subunit.
Subunit structure	Homotrimer. Ref.2
Sequence similarities	Belongs to the HpcH/HpaI aldolase family. Citrate lyase beta subunit-like subfamily.
Caution	This organism lacks the other subunits that are necessary for ATP-independent citrate lyase activity. Even though this protein has clear similarity to citrate lyase beta subunit, it is expected to have a somewhat different enzyme activity.

Ontologies

Keywords
Ligand	Magnesium Metal-binding
Molecular function	Lyase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular aromatic compound metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	citrate lyase complex Inferred from electronic annotation. Source: InterPro
Molecular function	citrate (pro-3S)-lyase activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 284

284

Citrate lyase subunit beta-like protein

PRO_0000286387

Sites

Metal binding

129

1

Magnesium

Metal binding

155

1

Magnesium

Binding site

74

1

Substrate

Binding site

129

1

Substrate

Secondary structure

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284

Helix Strand Turn

Details...

Beta strand

9 – 15

7

Helix

19 – 24

6

Turn

25 – 28

4

Beta strand

31 – 38

8

Helix

45 – 65

21

Beta strand

69 – 74

6

Helix

83 – 86

4

Helix

87 – 89

3

Beta strand

94 – 99

6

Helix

105 – 117

13

Beta strand

124 – 128

5

Helix

131 – 135

5

Helix

137 – 141

5

Beta strand

146 – 151

6

Helix

153 – 160

8

Helix

169 – 171

3

Helix

172 – 185

14

Beta strand

188 – 191

4

Helix

200 – 212

13

Beta strand

216 – 222

7

Helix

223 – 230

8

Sequences

	Sequence		Length	Mass (Da)
	Q9RUZ0 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: F95B6C9B20F89EC5	FASTA	284	29,980
	10 20 30 40 50 60 MNAPPALLRS VLFAPGNRAD LIAKLPRSAP DAVVIDLEDA VPGTAEAKAA ARPVAHDAAR 70 80 90 100 110 120 DLIAAAPHLA VFVRVNALHS PYFEDDLSVL TPELSGVVVP KLEMGAEARQ VAQMLQERSL 130 140 150 160 170 180 PLPILAGLET GAGVWNAREI MEVPEVAWAY FGAEDYTTDL GGKRTPGGLE VLYARSQVAL 190 200 210 220 230 240 AARLTGVAAL DIVVTALNDP ETFRADAEQG RALGYSGKLC IHPAQVALAH EYFGPTEADR 250 260 270 280 ARARALLDAA AAAAQRGHGA FSFEGQMVDE PMLAKARTLL SHEA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of the radioresistant bacterium Deinococcus radiodurans R1." White O., Eisen J.A., Heidelberg J.F., Hickey E.K., Peterson J.D., Dodson R.J., Haft D.H., Gwinn M.L., Nelson W.C., Richardson D.L., Moffat K.S., Qin H., Jiang L., Pamphile W., Crosby M., Shen M., Vamathevan J.J., Lam P. , McDonald L.A., Utterback T.R., Zalewski C., Makarova K.S., Aravind L., Daly M.J., Minton K.W., Fleischmann R.D., Ketchum K.A., Nelson K.E., Salzberg S.L., Smith H.O., Venter J.C., Fraser C.M. Science 286:1571-1577(1999) [PubMed: 10567266] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422.
[2]	"Crystal structure of citrate lyase beta subunit." New York structural genomics research consortium (NYSGRC) Submitted (JAN-2005) to the PDB data bank Cited for: X-RAY CRYSTALLOGRAPHY (1.84 ANGSTROMS) IN COMPLEX WITH MAGNESIUM IONS AND SUBSTRATE ANALOG, SUBUNIT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AE000513 Genomic DNA. Translation: AAF10813.1.

PIR

F75418.

RefSeq

NP_294964.1. NC_001263.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1SGJ	X-ray	1.84	A/B/C	1-284	[»]

ProteinModelPortal

Q9RUZ0.

SMR

Q9RUZ0. Positions 4-234.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

1798759.

GenomeReviews

Gene locus DR_1240 in contig AE000513_GR.

KEGG

dra:DR_1240.

NMPDR

fig|243230.1.peg.1423.

PATRIC

21630068. VBIDeiRad64572_1435.

TIGR

DR_1240.

Phylogenomic databases

HOGENOM

HBG676713.

OMA

VVAMREI.

PhylomeDB

Q9RUZ0.

ProtClustDB

CLSK444959.

Enzyme and pathway databases

BioCyc

DRAD243230:DR_1240-MONOMER.

Family and domain databases

InterPro

IPR005000. Aldehyde-lyase_domain.
IPR011206. Citrate_lyase_beta.
IPR015813. Pyrv/PenolPyrv_Kinase.
[Graphical view]

Gene3D

G3DSA:3.20.20.60. Pyrv/PenolPyrv_Kinase_cat. 1 hit.

KO

K01644.

PANTHER

PTHR11105. PTHR11105. 1 hit.

Pfam

PF03328. HpcH_HpaI. 1 hit.
[Graphical view]

PIRSF

PIRSF015582. Cit_lyase_B. 1 hit.

SUPFAM

SSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.

ProtoNet

Search...

Entry information

Entry name

CITEL_DEIRA

Accession

Primary (citable) accession number: Q9RUZ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
Last sequence update:	May 1, 2000
Last modified:	January 25, 2012
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program