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Q9RUP8

- SYI_DEIRA

UniProt

Q9RUP8 - SYI_DEIRA

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei640 – 6401ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciDRAD243230:GH46-1364-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:DR_1335
OrganismiDeinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
Taxonomic identifieri243230 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciDeinococcalesDeinococcaceaeDeinococcus
ProteomesiUP000002524: Chromosome I

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10781078Isoleucine--tRNA ligasePRO_0000098542Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi243230.DR_1335.

Structurei

3D structure databases

ProteinModelPortaliQ9RUP8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi52 – 6211"HIGH" regionAdd
BLAST
Motifi637 – 6415"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
InParanoidiQ9RUP8.
KOiK01870.
OMAiRVEHMVE.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9RUP8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTKRTFAPVP TQPNFRQLEA DILQKWKDEQ VFEQTQTRPA PAGEFVFYEG
60 70 80 90 100
PPTANGKPAL HHVLARSFKD LFPRFKVMQG HHVTRKGGWD THGLPVEISV
110 120 130 140 150
EKKLGWLGRN HGASREELEE FNRLCRTSVW ETIQDWNELT ERMGYWLDLG
160 170 180 190 200
DPYITYQNSY VESVWNLLRR LHEKGLVAQD YKVVPLSPRI SSTLSRAELG
210 220 230 240 250
EVDSYRMVDD PSVYVRLPIV WDTLPERAHA ALSSLSGEQR QGLSLLVWTT
260 270 280 290 300
TPWTLPSNTL AAVNPDLDYV VADSPSGRVI VAEGAVERLS ALHKDAAPLE
310 320 330 340 350
VLARFKGRDL EWVEYEPPFP EVASQLGVVS ELHERRDGKP VLHFVVMADF
360 370 380 390 400
VSDVDGSGVA HEAPAYGAED LEVARAYGVP LMFGVDDHGI LQVTHEQGKF
410 420 430 440 450
FKDADKGLIA DMKARGLMFW AGTLKHRYPF HDRTGDPILY FAKKGWYIRT
460 470 480 490 500
SQVAGEMLAQ NEKINWVPGN IKHGRFGNWL EGNVDWAISR ERYWGTPLPF
510 520 530 540 550
WQSESGQLRV IGSVAELSEL AGRDLSDLDL HRPYIDDITF TLDGEEYRRV
560 570 580 590 600
PEVLDVWFDS GSMPYAQWGL LLNEQGEAVR GAEQFAKHYP ADYICEAIDQ
610 620 630 640 650
TRGWFYSLHA ISTMLYDQPA YKNVICLGHI VDEKGLKMSK SKGNVVAPLP
660 670 680 690 700
LFDQYGADSV RWYMFMASDP GDQKRFSERL VAEAQRSYVN TLWNVYSFFV
710 720 730 740 750
LYANLDQPDL AAAPAVAERP EMDRWLLARL EETVRDVTAA LESYDARSGG
760 770 780 790 800
RALEGFVDDL SNWYVRRSRS RFWGEGGTVD TAAYATLHEA LLVVSQLTAP
810 820 830 840 850
FTPFLADALY RNLSGEESSV HLTPWPTVRA ERLDRKLTAD MAAVMKVVEL
860 870 880 890 900
GRAVRGAHNL KTRQPLAGVQ VRAASPEALD ALKRSQTQIM EELNVKAVTF
910 920 930 940 950
LEGDTDLVQY SLRPNLPVVG KQYGKQLPVL KKALTEADAR AVATAVQAGQ
960 970 980 990 1000
GFSVQADGVT FDLTPGSVLV DAKAPEGVAA AEDAGYLVAF DTALTPELVR
1010 1020 1030 1040 1050
EGLARDLVRA IQEARKAAGF EVQDRIALAL ELDGEALEAA QAWQDFIAGE
1060 1070
VLAEQVAYGS GEGFRAEVEG GAVTLKKL
Length:1,078
Mass (Da):120,273
Last modified:May 1, 2000 - v1
Checksum:iDF007ED70528F70F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000513 Genomic DNA. Translation: AAF10907.1.
PIRiE75407.
RefSeqiNP_295058.1. NC_001263.1.

Genome annotation databases

EnsemblBacteriaiAAF10907; AAF10907; DR_1335.
GeneIDi1798492.
KEGGidra:DR_1335.
PATRICi21630266. VBIDeiRad64572_1531.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE000513 Genomic DNA. Translation: AAF10907.1 .
PIRi E75407.
RefSeqi NP_295058.1. NC_001263.1.

3D structure databases

ProteinModelPortali Q9RUP8.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 243230.DR_1335.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAF10907 ; AAF10907 ; DR_1335 .
GeneIDi 1798492.
KEGGi dra:DR_1335.
PATRICi 21630266. VBIDeiRad64572_1531.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
InParanoidi Q9RUP8.
KOi K01870.
OMAi RVEHMVE.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci DRAD243230:GH46-1364-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422.

Entry informationi

Entry nameiSYI_DEIRA
AccessioniPrimary (citable) accession number: Q9RUP8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: May 1, 2000
Last modified: October 29, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3