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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei640ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:DR_1335
OrganismiDeinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
Taxonomic identifieri243230 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciDeinococcalesDeinococcaceaeDeinococcus
Proteomesi
  • UP000002524 Componenti: Chromosome I

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000985421 – 1078Isoleucine--tRNA ligaseAdd BLAST1078

Proteomic databases

PRIDEiQ9RUP8

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi243230.DR_1335

Structurei

3D structure databases

ProteinModelPortaliQ9RUP8
SMRiQ9RUP8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi52 – 62"HIGH" regionAdd BLAST11
Motifi637 – 641"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
HOGENOMiHOG000246403
InParanoidiQ9RUP8
KOiK01870
OMAiKMMAPFT
OrthoDBiPOG091H028I

Family and domain databases

CDDicd07961 Anticodon_Ia_Ile_ABEc, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02003 Ile_tRNA_synth_type2, 1 hit
InterProiView protein in InterPro
IPR002300 aa-tRNA-synth_Ia
IPR033709 Anticodon_Ile_ABEc
IPR002301 Ile-tRNA-ligase
IPR023586 Ile-tRNA-ligase_type2
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit

Sequencei

Sequence statusi: Complete.

Q9RUP8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKRTFAPVP TQPNFRQLEA DILQKWKDEQ VFEQTQTRPA PAGEFVFYEG
60 70 80 90 100
PPTANGKPAL HHVLARSFKD LFPRFKVMQG HHVTRKGGWD THGLPVEISV
110 120 130 140 150
EKKLGWLGRN HGASREELEE FNRLCRTSVW ETIQDWNELT ERMGYWLDLG
160 170 180 190 200
DPYITYQNSY VESVWNLLRR LHEKGLVAQD YKVVPLSPRI SSTLSRAELG
210 220 230 240 250
EVDSYRMVDD PSVYVRLPIV WDTLPERAHA ALSSLSGEQR QGLSLLVWTT
260 270 280 290 300
TPWTLPSNTL AAVNPDLDYV VADSPSGRVI VAEGAVERLS ALHKDAAPLE
310 320 330 340 350
VLARFKGRDL EWVEYEPPFP EVASQLGVVS ELHERRDGKP VLHFVVMADF
360 370 380 390 400
VSDVDGSGVA HEAPAYGAED LEVARAYGVP LMFGVDDHGI LQVTHEQGKF
410 420 430 440 450
FKDADKGLIA DMKARGLMFW AGTLKHRYPF HDRTGDPILY FAKKGWYIRT
460 470 480 490 500
SQVAGEMLAQ NEKINWVPGN IKHGRFGNWL EGNVDWAISR ERYWGTPLPF
510 520 530 540 550
WQSESGQLRV IGSVAELSEL AGRDLSDLDL HRPYIDDITF TLDGEEYRRV
560 570 580 590 600
PEVLDVWFDS GSMPYAQWGL LLNEQGEAVR GAEQFAKHYP ADYICEAIDQ
610 620 630 640 650
TRGWFYSLHA ISTMLYDQPA YKNVICLGHI VDEKGLKMSK SKGNVVAPLP
660 670 680 690 700
LFDQYGADSV RWYMFMASDP GDQKRFSERL VAEAQRSYVN TLWNVYSFFV
710 720 730 740 750
LYANLDQPDL AAAPAVAERP EMDRWLLARL EETVRDVTAA LESYDARSGG
760 770 780 790 800
RALEGFVDDL SNWYVRRSRS RFWGEGGTVD TAAYATLHEA LLVVSQLTAP
810 820 830 840 850
FTPFLADALY RNLSGEESSV HLTPWPTVRA ERLDRKLTAD MAAVMKVVEL
860 870 880 890 900
GRAVRGAHNL KTRQPLAGVQ VRAASPEALD ALKRSQTQIM EELNVKAVTF
910 920 930 940 950
LEGDTDLVQY SLRPNLPVVG KQYGKQLPVL KKALTEADAR AVATAVQAGQ
960 970 980 990 1000
GFSVQADGVT FDLTPGSVLV DAKAPEGVAA AEDAGYLVAF DTALTPELVR
1010 1020 1030 1040 1050
EGLARDLVRA IQEARKAAGF EVQDRIALAL ELDGEALEAA QAWQDFIAGE
1060 1070
VLAEQVAYGS GEGFRAEVEG GAVTLKKL
Length:1,078
Mass (Da):120,273
Last modified:May 1, 2000 - v1
Checksum:iDF007ED70528F70F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000513 Genomic DNA Translation: AAF10907.1
PIRiE75407
RefSeqiNP_295058.1, NC_001263.1
WP_010887976.1, NZ_CP015081.1

Genome annotation databases

EnsemblBacteriaiAAF10907; AAF10907; DR_1335
GeneIDi1798492
KEGGidra:DR_1335
PATRICifig|243230.17.peg.1531

Similar proteinsi

Entry informationi

Entry nameiSYI_DEIRA
AccessioniPrimary (citable) accession number: Q9RUP8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: May 1, 2000
Last modified: May 23, 2018
This is version 108 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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