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Q9RUP8

- SYI_DEIRA

UniProt

Q9RUP8 - SYI_DEIRA

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Zinc.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei640 – 6401ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciDRAD243230:GH46-1364-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:DR_1335
    OrganismiDeinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
    Taxonomic identifieri243230 [NCBI]
    Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciDeinococcalesDeinococcaceaeDeinococcus
    ProteomesiUP000002524: Chromosome I

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10781078Isoleucine--tRNA ligasePRO_0000098542Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi243230.DR_1335.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9RUP8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi52 – 6211"HIGH" regionAdd
    BLAST
    Motifi637 – 6415"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246403.
    KOiK01870.
    OMAiRVEHMVE.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9RUP8-1 [UniParc]FASTAAdd to Basket

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    MTKRTFAPVP TQPNFRQLEA DILQKWKDEQ VFEQTQTRPA PAGEFVFYEG     50
    PPTANGKPAL HHVLARSFKD LFPRFKVMQG HHVTRKGGWD THGLPVEISV 100
    EKKLGWLGRN HGASREELEE FNRLCRTSVW ETIQDWNELT ERMGYWLDLG 150
    DPYITYQNSY VESVWNLLRR LHEKGLVAQD YKVVPLSPRI SSTLSRAELG 200
    EVDSYRMVDD PSVYVRLPIV WDTLPERAHA ALSSLSGEQR QGLSLLVWTT 250
    TPWTLPSNTL AAVNPDLDYV VADSPSGRVI VAEGAVERLS ALHKDAAPLE 300
    VLARFKGRDL EWVEYEPPFP EVASQLGVVS ELHERRDGKP VLHFVVMADF 350
    VSDVDGSGVA HEAPAYGAED LEVARAYGVP LMFGVDDHGI LQVTHEQGKF 400
    FKDADKGLIA DMKARGLMFW AGTLKHRYPF HDRTGDPILY FAKKGWYIRT 450
    SQVAGEMLAQ NEKINWVPGN IKHGRFGNWL EGNVDWAISR ERYWGTPLPF 500
    WQSESGQLRV IGSVAELSEL AGRDLSDLDL HRPYIDDITF TLDGEEYRRV 550
    PEVLDVWFDS GSMPYAQWGL LLNEQGEAVR GAEQFAKHYP ADYICEAIDQ 600
    TRGWFYSLHA ISTMLYDQPA YKNVICLGHI VDEKGLKMSK SKGNVVAPLP 650
    LFDQYGADSV RWYMFMASDP GDQKRFSERL VAEAQRSYVN TLWNVYSFFV 700
    LYANLDQPDL AAAPAVAERP EMDRWLLARL EETVRDVTAA LESYDARSGG 750
    RALEGFVDDL SNWYVRRSRS RFWGEGGTVD TAAYATLHEA LLVVSQLTAP 800
    FTPFLADALY RNLSGEESSV HLTPWPTVRA ERLDRKLTAD MAAVMKVVEL 850
    GRAVRGAHNL KTRQPLAGVQ VRAASPEALD ALKRSQTQIM EELNVKAVTF 900
    LEGDTDLVQY SLRPNLPVVG KQYGKQLPVL KKALTEADAR AVATAVQAGQ 950
    GFSVQADGVT FDLTPGSVLV DAKAPEGVAA AEDAGYLVAF DTALTPELVR 1000
    EGLARDLVRA IQEARKAAGF EVQDRIALAL ELDGEALEAA QAWQDFIAGE 1050
    VLAEQVAYGS GEGFRAEVEG GAVTLKKL 1078
    Length:1,078
    Mass (Da):120,273
    Last modified:May 1, 2000 - v1
    Checksum:iDF007ED70528F70F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000513 Genomic DNA. Translation: AAF10907.1.
    PIRiE75407.
    RefSeqiNP_295058.1. NC_001263.1.
    WP_010887976.1. NC_001263.1.

    Genome annotation databases

    EnsemblBacteriaiAAF10907; AAF10907; DR_1335.
    GeneIDi1798492.
    KEGGidra:DR_1335.
    PATRICi21630266. VBIDeiRad64572_1531.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000513 Genomic DNA. Translation: AAF10907.1 .
    PIRi E75407.
    RefSeqi NP_295058.1. NC_001263.1.
    WP_010887976.1. NC_001263.1.

    3D structure databases

    ProteinModelPortali Q9RUP8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243230.DR_1335.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAF10907 ; AAF10907 ; DR_1335 .
    GeneIDi 1798492.
    KEGGi dra:DR_1335.
    PATRICi 21630266. VBIDeiRad64572_1531.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246403.
    KOi K01870.
    OMAi RVEHMVE.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci DRAD243230:GH46-1364-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422.

    Entry informationi

    Entry nameiSYI_DEIRA
    AccessioniPrimary (citable) accession number: Q9RUP8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 20, 2005
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 85 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3