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Q9RUF5 (PUR2_DEIRA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoribosylamine--glycine ligase

EC=6.3.4.13
Alternative name(s):
GARS
Glycinamide ribonucleotide synthetase
Phosphoribosylglycinamide synthetase
Gene names
Name:purD
Ordered Locus Names:DR_1431
OrganismDeinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422) [Reference proteome] [HAMAP]
Taxonomic identifier243230 [NCBI]
Taxonomic lineageBacteriaDeinococcus-ThermusDeinococciDeinococcalesDeinococcaceaeDeinococcus

Protein attributes

Sequence length415 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP-Rule MF_00138

Sequence similarities

Belongs to the GARS family.

Contains 1 ATP-grasp domain.

Sequence caution

The sequence AAF10999.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 415415Phosphoribosylamine--glycine ligase HAMAP-Rule MF_00138
PRO_0000151447

Regions

Domain104 – 304201ATP-grasp
Nucleotide binding130 – 18657ATP By similarity

Sites

Metal binding2741Magnesium or manganese By similarity
Metal binding2761Magnesium or manganese By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9RUF5 [UniParc].

Last modified March 27, 2002. Version 2.
Checksum: 32211639808CDD13

FASTA41543,351
        10         20         30         40         50         60 
MKVLVIGSGG REHAIVDACA RAGHEVLCTP GNPGIAAQAR LLASPQDAPT LADLAVREGA 

        70         80         90        100        110        120 
DVVIVGPEAY LAAGVVDECE RRGVPAFGPS QAASRLEGDK AWSKAFMVRH GIPTAQHRSF 

       130        140        150        160        170        180 
DTLDAALSHA ATQTPPIVVK DAGLKAGKGV TIAHSVAEAE AALREIFTQT GAQAVIEDFM 

       190        200        210        220        230        240 
TGQEVSILAL TDGKRYALTP PSQDHKTIYE GDTGPMTGGM GVICPFPVGE EQLNIIRRDI 

       250        260        270        280        290        300 
VEKTLAGMRA EGIPFRGVLY AGLMLTPQGP KVVEFNARFG DPEAEAVLPL LESDLAQHAL 

       310        320        330        340        350        360 
DAARGQLDPA QVRFRDGASA VVILAAPGYP AEPRKGIVLD IPADSPEAKV FHAGTTERDG 

       370        380        390        400        410 
QLVSSGGRVL AVTGLGESRE DALSRAYALA DCIGFVGAQL RRDIGFRIGL GGKPN 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE000513 Genomic DNA. Translation: AAF10999.1. Different initiation.
PIRE75397.
RefSeqNP_295154.1. NC_001263.1.

3D structure databases

ProteinModelPortalQ9RUF5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243230.DR_1431.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAF10999; AAF10999; DR_1431.
GeneID1797612.
KEGGdra:DR_1431.
PATRIC21630458. VBIDeiRad64572_1627.

Phylogenomic databases

eggNOGCOG0151.
HOGENOMHOG000033463.
KOK01945.
OMADPEAMNV.
OrthoDBEOG69SKD1.
ProtClustDBPRK00885.

Enzyme and pathway databases

BioCycDRAD243230:GH46-1460-MONOMER.
UniPathwayUPA00074; UER00125.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPMF_00138. GARS.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR00877. purD. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR2_DEIRA
AccessionPrimary (citable) accession number: Q9RUF5
Entry history
Integrated into UniProtKB/Swiss-Prot: March 27, 2002
Last sequence update: March 27, 2002
Last modified: February 19, 2014
This is version 90 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways