Reviewed, UniProtKB/Swiss-Prot Q9RUF5 (PUR2_DEIRA)

Names and origin

Protein names

Recommended name:
    Phosphoribosylamine--glycine ligase
    EC=6.3.4.13
Alternative name(s):
    GARS
    Glycinamide ribonucleotide synthetase
    Phosphoribosylglycinamide synthetase

Gene names

Name:	purD
Ordered Locus Names:	DR_1431

Organism

Deinococcus radiodurans [Complete proteome] [HAMAP]

Taxonomic identifier

1299 [NCBI]

Taxonomic lineage

Bacteria › Deinococcus-Thermus › Deinococci › Deinococcales › Deinococcaceae › Deinococcus

Protein attributes

Sequence length	415 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP MF_00138
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP MF_00138
Sequence similarities	Belongs to the GARS family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine base biosynthetic process Inferred from electronic annotation. Source: InterPro purine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylamine-glycine ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 415	415	Phosphoribosylamine--glycine ligase HAMAP MF_00138		PRO_0000151447
Regions
	Domain	104 – 304	201	ATP-grasp
	Nucleotide binding	130 – 186	57	ATP By similarity
Sites
	Metal binding	274	1	Magnesium or manganese By similarity
	Metal binding	276	1	Magnesium or manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9RUF5-1 [UniParc]. Last modified March 27, 2002. Version 2. Checksum: 32211639808CDD13	FASTA	415	43,351
	10 20 30 40 50 60 MKVLVIGSGG REHAIVDACA RAGHEVLCTP GNPGIAAQAR LLASPQDAPT LADLAVREGA 70 80 90 100 110 120 DVVIVGPEAY LAAGVVDECE RRGVPAFGPS QAASRLEGDK AWSKAFMVRH GIPTAQHRSF 130 140 150 160 170 180 DTLDAALSHA ATQTPPIVVK DAGLKAGKGV TIAHSVAEAE AALREIFTQT GAQAVIEDFM 190 200 210 220 230 240 TGQEVSILAL TDGKRYALTP PSQDHKTIYE GDTGPMTGGM GVICPFPVGE EQLNIIRRDI 250 260 270 280 290 300 VEKTLAGMRA EGIPFRGVLY AGLMLTPQGP KVVEFNARFG DPEAEAVLPL LESDLAQHAL 310 320 330 340 350 360 DAARGQLDPA QVRFRDGASA VVILAAPGYP AEPRKGIVLD IPADSPEAKV FHAGTTERDG 370 380 390 400 410 QLVSSGGRVL AVTGLGESRE DALSRAYALA DCIGFVGAQL RRDIGFRIGL GGKPN

References

[1]

"Genome sequence of the radioresistant bacterium Deinococcus radiodurans R1." White O., Eisen J.A., Heidelberg J.F., Hickey E.K., Peterson J.D., Dodson R.J., Haft D.H., Gwinn M.L., Nelson W.C., Richardson D.L., Moffat K.S., Qin H., Jiang L., Pamphile W., Crosby M., Shen M., Vamathevan J.J., Lam P. , McDonald L.A., Utterback T.R., Zalewski C., Makarova K.S., Aravind L., Daly M.J., Minton K.W., Fleischmann R.D., Ketchum K.A., Nelson K.E., Salzberg S.L., Smith H.O., Venter J.C., Fraser C.M. Science 286:1571-1577(1999) [PubMed: 10567266] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13939 / DSM 20539 / IFO 15346 / LMG 4051 / NCIB 9279 / R1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000513 Genomic DNA. Translation: AAF10999.1. Different initiation.
PIR	E75397.
RefSeq	NP_295154.1.
3D structure databases
HSSP	HSSP built from PDB template 1GSO based on UniProtKB P15640.
ModBase	Search...
Genome annotation databases
GeneID	1797612.
GenomeReviews	Gene locus DR_1431 in contig AE000513_GR.
KEGG	dra:DR_1431.
NMPDR	fig|243230.1.peg.1613.
TIGR	DR_1431.
Phylogenomic databases
HOGENOM	Q9RUF5.
Enzyme and pathway databases
BioCyc	DRAD243230:DR_1431-MON.
BRENDA	6.3.4.13. 96172.
Family and domain databases
HAMAP	MF_00138. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR013817. Pre-ATP_grasp. IPR020561. PRibGlycinamid_synth_ATP-grasp. IPR000115. PRibGlycinamide_synth. IPR020560. PRibGlycinamide_synth_C-dom. IPR020559. PRibGlycinamide_synth_CS. IPR020562. PRibGlycinamide_synth_N-dom. [Graphical view]
Gene3D	G3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.90.600.10. Gars. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
Pfam	PF01071. GARS_A. 1 hit. PF02843. GARS_C. 1 hit. PF02844. GARS_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00877. purD. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00184. GARS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR2_DEIRA

Accession

Primary (citable) accession number: Q9RUF5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 27, 2002
Last sequence update:	March 27, 2002
Last modified:	September 22, 2009
This is version 60 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)