Q9RUD2 (KPRS_DEIRA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable ribose-phosphate pyrophosphokinase
Short name=RPPK
EC=2.7.6.1

Alternative name(s):
Phosphoribosyl pyrophosphate synthase
Short name=P-Rib-PP synthase
Short name=PRPP synthase

Gene names

Name:	prs
Ordered Locus Names:	DR_1456

Organism

Deinococcus radiodurans

Taxonomic identifier

1299 [NCBI]

Taxonomic lineage

Bacteria › Deinococcus-Thermus › Deinococci › Deinococcales › Deinococcaceae › Deinococcus

Protein attributes

Sequence length	320 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_00583_B
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_00583_B
Pathway	Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1. HAMAP MF_00583_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00583_B.
Sequence similarities	Belongs to the ribose-phosphate pyrophosphokinase family.
Caution	This protein has lost two of the potential magnesium binding sites found in 5-phosphoribose 1-diphosphate synthases.
Sequence caution	The sequence AAF11025.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW kinase activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: InterPro ribose phosphate diphosphokinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 320	320	Probable ribose-phosphate pyrophosphokinase HAMAP MF_00583_B		PRO_0000141133
Regions
	Region	217 – 230	14	Binding of phosphoribosylpyrophosphate Potential
Sites
	Metal binding	134	1	Magnesium Potential
	Metal binding	147	1	Magnesium Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9RUD2 [UniParc]. Last modified April 13, 2004. Version 2. Checksum: CED355D89A7A2859	FASTA	320	34,239
	10 20 30 40 50 60 MNSRRSPLLV FSGQSNRPLA QAICDNLGVP LGRSRTEKFT NDNLIVHYEE SLREGDVFIV 70 80 90 100 110 120 QTFSTPVSDA IMELMLMIDA AKSASAGRVT AVIPYYSYAR SDKKDSPRIS IAGRLVADLL 130 140 150 160 170 180 QEAGADRVLT MTLHSPQVHG FFKVPVDHLS ADVVLSQHFK KCVPDAHNGV VLAPDAGSIK 190 200 210 220 230 240 RASQIARRLD SGLAMIDKER LSDTEVRPRA LIGDVDGKTV FIVDDEISTA GSLVETVSIA 250 260 270 280 290 300 RSMGAKDVYV AVTHGVYSGP AIERIAALDV TQVASCNTVL VPQDKLDRAG GKLAVLDVAP 310 320 LFASAIANIH TGASVSTLFT

References

[1]

"Genome sequence of the radioresistant bacterium Deinococcus radiodurans R1." White O., Eisen J.A., Heidelberg J.F., Hickey E.K., Peterson J.D., Dodson R.J., Haft D.H., Gwinn M.L., Nelson W.C., Richardson D.L., Moffat K.S., Qin H., Jiang L., Pamphile W., Crosby M., Shen M., Vamathevan J.J., Lam P. , McDonald L.A., Utterback T.R., Zalewski C., Makarova K.S., Aravind L., Daly M.J., Minton K.W., Fleischmann R.D., Ketchum K.A., Nelson K.E., Salzberg S.L., Smith H.O., Venter J.C., Fraser C.M. Science 286:1571-1577(1999) [PubMed: 10567266] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000513 Genomic DNA. Translation: AAF11025.1. Different initiation.
PIR	B75393.
RefSeq	NP_295179.1. NC_001263.1.
3D structure databases
ProteinModelPortal	Q9RUD2.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1797294.
GenomeReviews	Gene locus DR_1456 in contig AE000513_GR.
KEGG	dra:DR_1456.
NMPDR	fig|243230.1.peg.1638.
PATRIC	21630515. VBIDeiRad64572_1655.
TIGR	DR_1456.
Phylogenomic databases
HOGENOM	HBG519284.
OMA	NDCLEVQ.
PhylomeDB	Q9RUD2.
ProtClustDB	CLSK445074.
Enzyme and pathway databases
BioCyc	DRAD243230:DR_1456-MONOMER.
Family and domain databases
HAMAP	MF_00583_B. RibP_PPkinase_B. [Tree]
InterPro	IPR000836. PRibTrfase. IPR005946. Rib-P_diPkinase. [Graphical view]
KO	K00948.
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01251. RibP_PPkin. 1 hit.
PROSITE	PS00114. PRPP_SYNTHASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPRS_DEIRA

Accession

Primary (citable) accession number: Q9RUD2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 25, 2002
Last sequence update:	April 13, 2004
Last modified:	January 25, 2012
This is version 75 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program