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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei29S-adenosyl-L-methionineUniRule annotation1
Binding sitei55S-adenosyl-L-methionineUniRule annotation1
Active sitei105By similarity1
Binding sitei105S-adenosyl-L-methionineUniRule annotation1
Binding sitei109SubstrateUniRule annotation1
Binding sitei141SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:DR_1680
OrganismiDeinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
Taxonomic identifieri243230 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciDeinococcalesDeinococcaceaeDeinococcus
Proteomesi
  • UP000002524 Componenti: Chromosome I

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001713251 – 322tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST322

Interactioni

Protein-protein interaction databases

STRINGi243230.DR_1680.

Structurei

3D structure databases

ProteinModelPortaliQ9RTS6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CZ1. Bacteria.
COG0220. LUCA.
HOGENOMiHOG000099573.
InParanoidiQ9RTS6.
KOiK03439.
OMAiTKYALKW.
OrthoDBiPOG091H015P.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9RTS6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIFRLGDFRF PDDPARLYPD TPDRPWVLEI GFGDGRFWPH YARTFPEPPN
60 70 80 90 100
YLGVEISGVS LLKAHRRLKD AGLTNAVLTK LPAEVLVAQV IPHGSLDAII
110 120 130 140 150
VNFPDPWPKA GHEDHRLLRV PFFQVAASRL KPGGAALLTT DHDEYFEFAC
160 170 180 190 200
AQAEASGVMR VERVGPPPAA LETKYAQKWR DLGLGVNHAR FVPTRHDPVP
210 220 230 240 250
NGTFAPYSEE DPAVPHAVLT LPADFSPQHF DKLTVRGKTW TVVLLDLYAT
260 270 280 290 300
LRRGGWVALA HVVEGDLTQE VLVGITERED GTHLVRLAKF GGPIITPGVK
310 320
AAVGAVTEWL EGQGAVVKHR GY
Length:322
Mass (Da):35,470
Last modified:May 1, 2000 - v1
Checksum:iB1EDAC8115BAD4A1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000513 Genomic DNA. Translation: AAF11238.1.
PIRiF75365.
RefSeqiNP_295403.1. NC_001263.1.
WP_010888315.1. NZ_CP015081.1.

Genome annotation databases

EnsemblBacteriaiAAF11238; AAF11238; DR_1680.
GeneIDi1797706.
KEGGidra:DR_1680.
PATRICi21630988. VBIDeiRad64572_1889.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000513 Genomic DNA. Translation: AAF11238.1.
PIRiF75365.
RefSeqiNP_295403.1. NC_001263.1.
WP_010888315.1. NZ_CP015081.1.

3D structure databases

ProteinModelPortaliQ9RTS6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243230.DR_1680.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAF11238; AAF11238; DR_1680.
GeneIDi1797706.
KEGGidra:DR_1680.
PATRICi21630988. VBIDeiRad64572_1889.

Phylogenomic databases

eggNOGiENOG4105CZ1. Bacteria.
COG0220. LUCA.
HOGENOMiHOG000099573.
InParanoidiQ9RTS6.
KOiK03439.
OMAiTKYALKW.
OrthoDBiPOG091H015P.

Enzyme and pathway databases

UniPathwayiUPA00989.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRMB_DEIRA
AccessioniPrimary (citable) accession number: Q9RTS6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: May 1, 2000
Last modified: November 2, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.