Reviewed, UniProtKB/Swiss-Prot Q9RTB7 (GLGB_DEIRA)

Names and origin

Protein names

Recommended name:
    1,4-alpha-glucan-branching enzyme
    EC=2.4.1.18
Alternative name(s):
    Glycogen-branching enzyme
      Short name=BE
    1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase

Gene names

Name:	glgB
Ordered Locus Names:	DR_1848

Organism

Deinococcus radiodurans [Complete proteome] [HAMAP]

Taxonomic identifier

1299 [NCBI]

Taxonomic lineage

Bacteria › Deinococcus-Thermus › Deinococci › Deinococcales › Deinococcaceae › Deinococcus

Protein attributes

Sequence length	705 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity.
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
Biological process	Glycogen biosynthesis
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: HAMAP cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 705	705	1,4-alpha-glucan-branching enzyme HAMAP MF_00685		PRO_0000188700
Sites
	Active site	204	1	By similarity
	Active site	239	1	By similarity
	Active site	244	1	By similarity
	Active site	307	1	By similarity
	Active site	309	1	By similarity
	Active site	360	1	By similarity
	Active site	426	1	By similarity
	Active site	427	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9RTB7-1 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 6122BD1961CE6230	FASTA	705	80,006
	10 20 30 40 50 60 MTFPLPLDHE HLQKLVTADL VRPDHLLGAH PTTEHGVQGV RFAVWAPNAQ HVSVVGDFND 70 80 90 100 110 120 WNGFDHPLQR LDFGFWGAFV PAAQPGQRYK FRVTGAGGQT VDKTDPYGTF FEVRPNNASI 130 140 150 160 170 180 IWQQDFEWTD AGWLEQRARR GQALEDPISI YECHVGSWAR RDDGWFLNYR DLAHRLGEYV 190 200 210 220 230 240 TYMGYTHVEL LGVMEHPFDG SWGYQVTGYY APTSRLGSPE DFKYLVNHLH SLGIGVLLDW 250 260 270 280 290 300 VPGHFPTDEF ALAHFDGSPL YEYADPRKGY HYDWNTYIFD YGRNEVVMFL IGSALKWVQD 310 320 330 340 350 360 YHVDGLRVDA VASMLYLDFS RTEWVPNIYG GRENLEAIAF LKRLNEVAHH MAPGCLMIAE 370 380 390 400 410 420 ESTSFPGVTT PTPEGLGFDY KWAMGWMNDS LAYFEQDPIY RKYDHHKLTF FNVYRTSENY 430 440 450 460 470 480 VLAISHDEVV HLKKPMVLKH PGDWYAQRAD YRAFLAMMWT TPGKKLLFMG QDFAQGSEWN 490 500 510 520 530 540 HDAPLPWFHA DQPDHRGVMN LVRRLNELYR ERPDWHTGDA REEGMAWISA DDTDNSVYAY 550 560 570 580 590 600 ARRDTYSGAW SLVIANLTPV YREDYVIGVP QGGEYRVLLS TDDGEFGGFG TQQPDLSARD 610 620 630 640 650 660 EAAHGQAHAL HLNLPPSSVL VLEPVAAAPV KGLVSRQELT PELREVARQS AQAVQVERAA 670 680 690 700 DPRPNEQQRL VAETPAHEGG RSAPADAAES AEQKPDDEQK GGKKA

References

[1]

"Genome sequence of the radioresistant bacterium Deinococcus radiodurans R1." White O., Eisen J.A., Heidelberg J.F., Hickey E.K., Peterson J.D., Dodson R.J., Haft D.H., Gwinn M.L., Nelson W.C., Richardson D.L., Moffat K.S., Qin H., Jiang L., Pamphile W., Crosby M., Shen M., Vamathevan J.J., Lam P. , McDonald L.A., Utterback T.R., Zalewski C., Makarova K.S., Aravind L., Daly M.J., Minton K.W., Fleischmann R.D., Ketchum K.A., Nelson K.E., Salzberg S.L., Smith H.O., Venter J.C., Fraser C.M. Science 286:1571-1577(1999) [PubMed: 10567266] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13939 / DSM 20539 / IFO 15346 / LMG 4051 / NCIB 9279 / R1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE000513 Genomic DNA. Translation: AAF11402.1.
PIR	D75345.
RefSeq	NP_295571.1.
3D structure databases
HSSP	HSSP built from PDB template 1M7X based on UniProtKB P07762.
ModBase	Search...
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Genome annotation databases
GeneID	1798355.
GenomeReviews	Gene locus DR_1848 in contig AE000513_GR.
KEGG	dra:DR_1848.
NMPDR	fig|243230.1.peg.2030.
TIGR	DR_1848.
Phylogenomic databases
HOGENOM	Q9RTB7.
OMA	TANALYW.
Enzyme and pathway databases
BioCyc	DRAD243230:DR_1848-MON.
BRENDA	2.4.1.18. 96172.
Family and domain databases
HAMAP	MF_00685. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz_core. IPR006048. A-amylase_b_C. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_sg_catalytic. IPR013783. Ig-like_fold. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
TIGRFAMs	TIGR01515. branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLGB_DEIRA

Accession

Primary (citable) accession number: Q9RTB7

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 15, 2003
Last sequence update:	May 1, 2000
Last modified:	November 3, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)