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Protein

Trigger factor

Gene

tig

Organism
Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation. Functions as a peptidyl-prolyl cis-trans isomerase (By similarity). Probably changes conformation upon binding to the ribosome (maybe in particular due to interaction with L24, PubMed:16271892), exposing a hydrophobic crevice that is probably important for its chaperone activity (PubMed:16091460 and PubMed:16271892).By similarity

Catalytic activityi

Peptidylproline (omega=180) = peptidylproline (omega=0).

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone, Isomerase, Rotamase

Keywords - Biological processi

Cell cycle, Cell division

Keywords - Ligandi

RNA-binding, rRNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Trigger factor (EC:5.2.1.8)
Short name:
TF
Alternative name(s):
PPIase
Gene namesi
Name:tig
Ordered Locus Names:DR_1948
OrganismiDeinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
Taxonomic identifieri243230 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciDeinococcalesDeinococcaceaeDeinococcus
Proteomesi
  • UP000002524 Componenti: Chromosome I

Subcellular locationi

  • Cytoplasm

  • Note: About half TF is bound to the ribosome near the polypeptide exit tunnel while the other half is free in the cytoplasm.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001793442 – 465Trigger factorAdd BLAST464

Interactioni

Subunit structurei

Binds to the 50S ribosomal subunit via interactions with ribosomal protein L23. Also interacts with 23S rRNA and proteins L24 and L29 when complexed with the ribosome (PubMed:16091460 and PubMed:16271892).2 Publications

Protein-protein interaction databases

STRINGi243230.DR_1948.

Structurei

Secondary structure

1465
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi12 – 16Combined sources5
Helixi20 – 22Combined sources3
Helixi24 – 35Combined sources12
Helixi53 – 56Combined sources4
Turni57 – 60Combined sources4
Helixi63 – 81Combined sources19
Beta strandi98 – 100Combined sources3
Beta strandi104 – 108Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2AARX-ray3.5072-114[»]
2D3OX-ray3.3511-112[»]
ProteinModelPortaliQ9RT21.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9RT21.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini160 – 235PPIase FKBP-typeAdd BLAST76

Domaini

Consists of 3 domains; the N-terminus binds the ribosome, the middle domain has PPIase activity, while the C-terminus has intrinsic chaperone activity on its own.By similarity

Sequence similaritiesi

Belongs to the FKBP-type PPIase family. Tig subfamily.Curated
Contains 1 PPIase FKBP-type domain.Curated

Phylogenomic databases

eggNOGiENOG4105DEA. Bacteria.
COG0544. LUCA.
HOGENOMiHOG000099671.
InParanoidiQ9RT21.
KOiK03545.
OMAiPRIIDQR.
OrthoDBiPOG091H01T7.

Family and domain databases

Gene3Di1.10.3120.10. 1 hit.
3.30.70.1050. 1 hit.
HAMAPiMF_00303. Trigger_factor_Tig. 1 hit.
InterProiIPR005215. Trig_fac.
IPR008880. Trigger_fac_C.
IPR008881. Trigger_fac_ribosome-bd_bac.
IPR027304. Trigger_fact/SurA_dom.
[Graphical view]
PfamiPF05698. Trigger_C. 1 hit.
PF05697. Trigger_N. 1 hit.
[Graphical view]
PIRSFiPIRSF003095. Trigger_factor. 1 hit.
SUPFAMiSSF102735. SSF102735. 1 hit.
SSF109998. SSF109998. 1 hit.
TIGRFAMsiTIGR00115. tig. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9RT21-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAELISKEGN KVEFKVSVPA AEVNRAYDQV WAGLARDVRV PGFRPGKAPR
60 70 80 90 100
KVIENRVGKG YVESQVRDRL LETHYSQGLR ELGLNLVDAT VDPQDVQSGQ
110 120 130 140 150
AFEFTVKGET YPEVKLGDWQ GLKVSAQAPE ITDEVLEQTL SDLRERNASF
160 170 180 190 200
EKAERPIEAA DQVTIQELGE GDSEEGGSYP IYLDMAEEHV RNALLGKSAG
210 220 230 240 250
DVVDITVPAH QHGDHEHAEH TVRVKVVEVS SKKLQDLNDE FATSLNYESM
260 270 280 290 300
DKLRTDLREE LERRAQQEGD NLRREELVGH LVEGMTVEIP QALIDRRREG
310 320 330 340 350
MMSEIQDDLR RQGVQWKEYE AFMQEQGKLD EFEADLTKNA ETRVRRDLAL
360 370 380 390 400
EQLATDLNAQ VNEAEFNQTL MNLAQANGMN VQQLVQQLGQ DGVQSYYISL
410 420 430 440 450
LRERGLQRAL AQLSGEGQST EAASPKATGT EAAGTEQSEP AQTETAQNDA
460
GQTETAQSEG EQQSE
Length:465
Mass (Da):51,847
Last modified:January 23, 2007 - v3
Checksum:i475E073690C8E006
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000513 Genomic DNA. Translation: AAF11500.1.
PIRiD75334.
RefSeqiNP_295671.1. NC_001263.1.
WP_010888581.1. NZ_CP015081.1.

Genome annotation databases

EnsemblBacteriaiAAF11500; AAF11500; DR_1948.
GeneIDi1798866.
KEGGidra:DR_1948.
PATRICi21631548. VBIDeiRad64572_2168.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000513 Genomic DNA. Translation: AAF11500.1.
PIRiD75334.
RefSeqiNP_295671.1. NC_001263.1.
WP_010888581.1. NZ_CP015081.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2AARX-ray3.5072-114[»]
2D3OX-ray3.3511-112[»]
ProteinModelPortaliQ9RT21.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi243230.DR_1948.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAF11500; AAF11500; DR_1948.
GeneIDi1798866.
KEGGidra:DR_1948.
PATRICi21631548. VBIDeiRad64572_2168.

Phylogenomic databases

eggNOGiENOG4105DEA. Bacteria.
COG0544. LUCA.
HOGENOMiHOG000099671.
InParanoidiQ9RT21.
KOiK03545.
OMAiPRIIDQR.
OrthoDBiPOG091H01T7.

Miscellaneous databases

EvolutionaryTraceiQ9RT21.

Family and domain databases

Gene3Di1.10.3120.10. 1 hit.
3.30.70.1050. 1 hit.
HAMAPiMF_00303. Trigger_factor_Tig. 1 hit.
InterProiIPR005215. Trig_fac.
IPR008880. Trigger_fac_C.
IPR008881. Trigger_fac_ribosome-bd_bac.
IPR027304. Trigger_fact/SurA_dom.
[Graphical view]
PfamiPF05698. Trigger_C. 1 hit.
PF05697. Trigger_N. 1 hit.
[Graphical view]
PIRSFiPIRSF003095. Trigger_factor. 1 hit.
SUPFAMiSSF102735. SSF102735. 1 hit.
SSF109998. SSF109998. 1 hit.
TIGRFAMsiTIGR00115. tig. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTIG_DEIRA
AccessioniPrimary (citable) accession number: Q9RT21
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 109 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.