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Q9RSF0 (SYL_DEIRA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:DR_2174
OrganismDeinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422) [Reference proteome] [HAMAP]
Taxonomic identifier243230 [NCBI]
Taxonomic lineageBacteriaDeinococcus-ThermusDeinococciDeinococcalesDeinococcaceaeDeinococcus

Protein attributes

Sequence length823 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Sequence caution

The sequence AAF11720.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

regulation of translational fidelity

Inferred from electronic annotation. Source: GOC

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 823823Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000152009

Regions

Motif55 – 6511"HIGH" region HAMAP-Rule MF_00049
Motif590 – 5945"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5931ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9RSF0 [UniParc].

Last modified June 1, 2001. Version 2.
Checksum: 2E6445F39005A110

FASTA82392,613
        10         20         30         40         50         60 
MTQEATKPNI QEPRAERYNP HAIEQKWQGQ WQESGLYKFD ENAPGEKFYA LTMFPYPSGN 

        70         80         90        100        110        120 
LHIGHWYANV APDARARWLR MRGYNVLFPM AFDAFGLPAE NAAIKNNTNP ATWTYANIER 

       130        140        150        160        170        180 
MTGQFSRMGT MIDWSRKFAT CDPEYYRWNQ WFFIEFWKRG LAYKKGGLVN WCPKDQTVLA 

       190        200        210        220        230        240 
NEQVVNGHCE RCGTAVERRN LSQWYLKITD YAEELLDFSA TDMPEKVRAM QTNWIGKSVG 

       250        260        270        280        290        300 
AEVTFDTPAG PETVFTTRPD TLMGATFMVL APEHAKVKEL TTDEQRAEVE AYVAAAGRKT 

       310        320        330        340        350        360 
DVERQQEGEK TGVFTGSYAT HPISGHQLPI WVADYVLVTY GTGSIMAVPA HDERDFAFAK 

       370        380        390        400        410        420 
KFDLPIREVI RAEGSEGMGD QPSEPYSGEG QIVNSGEFDG MPGGKASIAA IIARLEERGV 

       430        440        450        460        470        480 
AKAKTTYRLR DWLFARQRYW GTPIPFVHCE KCGMQPVPED QLPVKLPENV AFTPTGQSPL 

       490        500        510        520        530        540 
KLDEEWKTTT CPCCGGPAER ETDTMDTFVD SSWYMYRYLS PNYDGGPFDP SKAGLLPVDL 

       550        560        570        580        590        600 
YTGGIEHAIL HLLYSRFWTK VMRDMGLTTQ SEPFARLRNQ GMVLGEDGEK MSKSRGNVVD 

       610        620        630        640        650        660 
PDDLVREYGA DTVRAFLMFI APWELGGPWD PQGINGPSKW LSRVWNVFFE EKVSGPEEKM 

       670        680        690        700        710        720 
QGADVRFAVH SALKKVNDDF ERMSFNTIIS TLMELTNALV KAKRSPVFGT PVWEEALDIF 

       730        740        750        760        770        780 
NRMLAPVVPH IAEEIWHERG QKGSVHTAQW PQVDEAAAVR DTVTIGVQVS GKVRGEVSIS 

       790        800        810        820 
KTASQEEALS AARAIAEVQK HLEGKTVVKE IYVPGRIINI VAK 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE000513 Genomic DNA. Translation: AAF11720.1. Different initiation.
PIRD75307.
RefSeqNP_295897.1. NC_001263.1.

3D structure databases

ProteinModelPortalQ9RSF0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243230.DR_2174.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAF11720; AAF11720; DR_2174.
GeneID1799430.
KEGGdra:DR_2174.
PATRIC21632026. VBIDeiRad64572_2398.

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycDRAD243230:GH46-2217-MONOMER.

Family and domain databases

Gene3D3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSYL_DEIRA
AccessionPrimary (citable) accession number: Q9RSF0
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: June 1, 2001
Last modified: February 19, 2014
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries