Q9RRX9 (ASPG_DEIRA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable L-asparaginase
Short name=L-ASNase
EC=3.5.1.1

Alternative name(s):
L-asparagine amidohydrolase

Gene names

Name:	ansA
Ordered Locus Names:	DR_2353

Organism

Deinococcus radiodurans

Taxonomic identifier

1299 [NCBI]

Taxonomic lineage

Bacteria › Deinococcus-Thermus › Deinococci › Deinococcales › Deinococcaceae › Deinococcus

Protein attributes

Sequence length	322 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	L-asparagine + H2O = L-aspartate + NH3.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the asparaginase 1 family.
Sequence caution	The sequence AAF11899.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	asparaginase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 322	322	Probable L-asparaginase		PRO_0000171078
Regions
	Region	85 – 86	2	Substrate binding By similarity
Sites
	Active site	16	1	O-isoaspartyl threonine intermediate By similarity
	Binding site	54	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9RRX9 [UniParc]. Last modified August 29, 2001. Version 2. Checksum: 0339A30B5A7E130E	FASTA	322	33,807
	10 20 30 40 50 60 MPASLPRLAL IHTGGTIASR PSPDGRGLTP QTPPALPGLE GVQVSEHQPF NLPSPHVTPA 70 80 90 100 110 120 HMQQLAHLIE QLAGGHDAVV VTHGTDTLEE TAFFLHLCLP AGLPVVLTGS MRHAEEVSWD 130 140 150 160 170 180 GPGNLLDAAQ VALCPQTAGR GPLVVFGGDI FDARTVTKVH TSAVDAFGGY PGPIGRIDRT 190 200 210 220 230 240 AAGPQVHYFA RPEPRPTFRP VTLERRVEIL YAYAGWQGEG YAGALERADG LVIAALGTGN 250 260 270 280 290 300 LPPELLPLIA ATDKPVVLAT RTHAGPILPV YGYAGGGATL VEAGAIPASF LNAHKARLLL 310 320 LVLLNLGASR EDIRRVFTQG VF

References

[1]

"Genome sequence of the radioresistant bacterium Deinococcus radiodurans R1." White O., Eisen J.A., Heidelberg J.F., Hickey E.K., Peterson J.D., Dodson R.J., Haft D.H., Gwinn M.L., Nelson W.C., Richardson D.L., Moffat K.S., Qin H., Jiang L., Pamphile W., Crosby M., Shen M., Vamathevan J.J., Lam P. , McDonald L.A., Utterback T.R., Zalewski C., Makarova K.S., Aravind L., Daly M.J., Minton K.W., Fleischmann R.D., Ketchum K.A., Nelson K.E., Salzberg S.L., Smith H.O., Venter J.C., Fraser C.M. Science 286:1571-1577(1999) [PubMed: 10567266] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE000513 Genomic DNA. Translation: AAF11899.1. Different initiation.
PIR	G75283.
RefSeq	NP_296074.1. NC_001263.1.
3D structure databases
ProteinModelPortal	Q9RRX9.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1798808.
GenomeReviews	Gene locus DR_2353 in contig AE000513_GR.
KEGG	dra:DR_2353.
NMPDR	fig|243230.1.peg.2533.
PATRIC	21632420. VBIDeiRad64572_2587.
TIGR	DR_2353.
Phylogenomic databases
HOGENOM	HBG497495.
OMA	IGRIDRT.
PhylomeDB	Q9RRX9.
ProtClustDB	CLSK445548.
Enzyme and pathway databases
BioCyc	DRAD243230:DR_2353-MONOMER.
Family and domain databases
InterPro	IPR006034. Asparaginase/glutaminase. IPR020827. Asparaginase/glutaminase_CS. [Graphical view]
KO	K01424.
PANTHER	PTHR11707. Asp/Glutamnse. 1 hit.
Pfam	PF00710. Asparaginase. 1 hit. [Graphical view]
PIRSF	PIRSF001220. L-ASNase_gatD. 1 hit.
PRINTS	PR00139. ASNGLNASE.
SMART	SM00870. Asparaginase. 1 hit. [Graphical view]
SUPFAM	SSF53774. Asp/Glutamnse. 1 hit.
PROSITE	PS00144. ASN_GLN_ASE_1. 1 hit. PS00917. ASN_GLN_ASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASPG_DEIRA

Accession

Primary (citable) accession number: Q9RRX9

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 29, 2001
Last sequence update:	August 29, 2001
Last modified:	January 25, 2012
This is version 76 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program