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Q9RRH3

- RQKA_DEIRA

UniProt

Q9RRH3 - RQKA_DEIRA

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Protein

DNA damage-responsive serine/threonine-protein kinase RqkA

Gene

rqkA

Organism
Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Plays an important role in radiation resistance and DNA double-strand break (DSB) repair. Involved in transcriptional regulation of genes important for bacterial stress response. Phosphorylates PprA in vitro.3 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.2 Publications

Cofactori

pyrroloquinoline quinone1 Publication

Enzyme regulationi

Autokinase activity is stimulated by DNA damage. Stimulated by PQQ and DNA ends in vitro.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei42 – 421ATPCurated
Active sitei137 – 1371Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi19 – 279ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. protein serine/threonine kinase activity Source: UniProtKB-KW

GO - Biological processi

  1. response to stress Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Stress response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciDRAD243230:GH46-2570-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA damage-responsive serine/threonine-protein kinase RqkA (EC:2.7.11.1)
Alternative name(s):
Radiation and pyrroloquinoline quinone inducible protein kinase
Gene namesi
Name:rqkA
Ordered Locus Names:DR_2518
OrganismiDeinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
Taxonomic identifieri243230 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciDeinococcalesDeinococcaceaeDeinococcus
ProteomesiUP000002524: Chromosome I

Pathology & Biotechi

Disruption phenotypei

Mutant exhibits higher sensitivity to different DNA-damaging agents. It shows altered phosphoprotein profile and impaired DSB repair. Deletion affects expression of genes involved in intermediary metabolism, stress response and growth under stressed conditions.2 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi42 – 421K → A: Lack of activity. 1 Publication
Mutagenesisi162 – 1621S → A: Almost no change in activity. 1 Publication
Mutagenesisi169 – 1691T → A: Strong decrease in activity. 1 Publication
Mutagenesisi171 – 1711S → A: Strong decrease in activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 668668DNA damage-responsive serine/threonine-protein kinase RqkAPRO_0000429795Add
BLAST

Post-translational modificationi

Autophosphorylated.

Keywords - PTMi

Phosphoprotein

Expressioni

Inductioni

Expression is induced in response to DNA damage.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi243230.DR_2518.

Structurei

3D structure databases

ProteinModelPortaliQ9RRH3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini13 – 272260Protein kinasePROSITE-ProRule annotationAdd
BLAST

Domaini

Contains an N-terminal catalytic kinase domain and a C-terminal PQQ binding domain.1 Publication

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000099391.
InParanoidiQ9RRH3.
OMAiPFEGEND.
OrthoDBiEOG6B35XT.

Family and domain databases

Gene3Di2.140.10.10. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR018391. PQQ_beta_propeller_repeat.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR027295. Quinonprotein_ADH-like_fam.
IPR011047. Quinonprotein_ADH-like_supfam.
IPR008266. Tyr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00564. PQQ. 7 hits.
[Graphical view]
SUPFAMiSSF50998. SSF50998. 2 hits.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9RRH3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPLTPGTLLA GRYELLALLG EGGSAQVYRA QDGLLGREVA LKVMHDYLPE
60 70 80 90 100
SDRSRFLREV RTLARLTHPG VVPVLDLGQE PEAGRPFFTM PLLTGGPITR
110 120 130 140 150
LGPLEDAPGP LARFLTAAAF ASRALHHVHS HGIVHRDLTP GNVLLDDTGL
160 170 180 190 200
PRIMDFGLVA LSEQTRHLTR SGVTLGTPAY MAPEQAKGGG VDARSDLYAL
210 220 230 240 250
GAVLYRVACG SPPFVGDSDQ SVLYQHVYEP VPDPRDLNPA VPDAVARVLL
260 270 280 290 300
WLLAKRADRR PQSGAALAHL WALARRDLWT THARGQYRGG RARTGEHPDG
310 320 330 340 350
PARVSDMQEL WSVALPGEVT WPAAVVGEGD LVAVGTRGGQ LVLTHTSGRP
360 370 380 390 400
FATYAARDEV TAPATLIGGH VLYGAWDGTL RRVELQSGSE VWRHQARAEF
410 420 430 440 450
TGAPTVWGGR LLAPSRDGHL HALSLRTGEL AWAYRAGGSL AASPLVWAGA
460 470 480 490 500
ALQCDETGWL HALDARSGTP LWKVEVGTVH ATPALLPGPP GTATLVIATW
510 520 530 540 550
EGEVHAIGLE VQNGRAALAG EDAIRWTYDV EDEVWASPAL TALDLPPDSG
560 570 580 590 600
AAPDASAAPG GVVVVAGWGG KVRGLRLADG EDLWERTLDG RVTASPVISA
610 620 630 640 650
GLVFLATEGG ELLALDVRNG EVRWTCRERS GVQATPLAAS GTLYVAFMDG
660
TLRAYRNAHP EWRSEQEG
Length:668
Mass (Da):71,126
Last modified:May 1, 2000 - v1
Checksum:i22997D8E4127BC92
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000513 Genomic DNA. Translation: AAF12057.1.
PIRiC75264.
RefSeqiNP_296238.1. NC_001263.1.

Genome annotation databases

EnsemblBacteriaiAAF12057; AAF12057; DR_2518.
GeneIDi1798368.
KEGGidra:DR_2518.
PATRICi21632771. VBIDeiRad64572_2760.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000513 Genomic DNA. Translation: AAF12057.1 .
PIRi C75264.
RefSeqi NP_296238.1. NC_001263.1.

3D structure databases

ProteinModelPortali Q9RRH3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 243230.DR_2518.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAF12057 ; AAF12057 ; DR_2518 .
GeneIDi 1798368.
KEGGi dra:DR_2518.
PATRICi 21632771. VBIDeiRad64572_2760.

Phylogenomic databases

HOGENOMi HOG000099391.
InParanoidi Q9RRH3.
OMAi PFEGEND.
OrthoDBi EOG6B35XT.

Enzyme and pathway databases

BioCyci DRAD243230:GH46-2570-MONOMER.

Family and domain databases

Gene3Di 2.140.10.10. 1 hit.
InterProi IPR011009. Kinase-like_dom.
IPR018391. PQQ_beta_propeller_repeat.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR027295. Quinonprotein_ADH-like_fam.
IPR011047. Quinonprotein_ADH-like_supfam.
IPR008266. Tyr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00564. PQQ. 7 hits.
[Graphical view ]
SUPFAMi SSF50998. SSF50998. 2 hits.
SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422.
  2. "Characterization of a DNA damage-inducible membrane protein kinase from Deinococcus radiodurans and its role in bacterial radioresistance and DNA strand break repair."
    Rajpurohit Y.S., Misra H.S.
    Mol. Microbiol. 77:1470-1482(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, COFACTOR, ENZYME REGULATION, INDUCTION, DOMAIN, AUTOPHOSPHORYLATION, DISRUPTION PHENOTYPE.
    Strain: ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422.
  3. "Structure-function study of deinococcal serine/threonine protein kinase implicates its kinase activity and DNA repair protein phosphorylation roles in radioresistance of Deinococcus radiodurans."
    Rajpurohit Y.S., Misra H.S.
    Int. J. Biochem. Cell Biol. 45:2541-2552(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, GENE NAME, MUTAGENESIS OF LYS-42; SER-162; THR-169 AND SER-171.
    Strain: ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422.
  4. "Pyrroloquinoline quinone and a quinoprotein kinase support gamma-radiation resistance in Deinococcus radiodurans and regulate gene expression."
    Rajpurohit Y.S., Desai S.S., Misra H.S.
    J. Basic Microbiol. 53:518-531(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN REGULATION OF GENES, DISRUPTION PHENOTYPE.
    Strain: ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422.

Entry informationi

Entry nameiRQKA_DEIRA
AccessioniPrimary (citable) accession number: Q9RRH3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 9, 2014
Last sequence update: May 1, 2000
Last modified: November 26, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3