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Q9RRH3

- RQKA_DEIRA

UniProt

Q9RRH3 - RQKA_DEIRA

Protein

DNA damage-responsive serine/threonine-protein kinase RqkA

Gene

rqkA

Organism
Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 1 (01 May 2000)
      Previous versions | rss
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    Functioni

    Plays an important role in radiation resistance and DNA double-strand break (DSB) repair. Involved in transcriptional regulation of genes important for bacterial stress response. Phosphorylates PprA in vitro.3 Publications

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.2 Publications

    Cofactori

    PQQ.1 Publication

    Enzyme regulationi

    Autokinase activity is stimulated by DNA damage. Stimulated by PQQ and DNA ends in vitro.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei42 – 421ATPCurated
    Active sitei137 – 1371Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi19 – 279ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. protein serine/threonine kinase activity Source: UniProtKB-KW
    3. protein tyrosine kinase activity Source: InterPro

    GO - Biological processi

    1. response to stress Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Stress response

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciDRAD243230:GH46-2570-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA damage-responsive serine/threonine-protein kinase RqkA (EC:2.7.11.1)
    Alternative name(s):
    Radiation and pyrroloquinoline quinone inducible protein kinase
    Gene namesi
    Name:rqkA
    Ordered Locus Names:DR_2518
    OrganismiDeinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
    Taxonomic identifieri243230 [NCBI]
    Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciDeinococcalesDeinococcaceaeDeinococcus
    ProteomesiUP000002524: Chromosome I

    Pathology & Biotechi

    Disruption phenotypei

    Mutant exhibits higher sensitivity to different DNA-damaging agents. It shows altered phosphoprotein profile and impaired DSB repair. Deletion affects expression of genes involved in intermediary metabolism, stress response and growth under stressed conditions.2 Publications

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi42 – 421K → A: Lack of activity. 1 Publication
    Mutagenesisi162 – 1621S → A: Almost no change in activity. 1 Publication
    Mutagenesisi169 – 1691T → A: Strong decrease in activity. 1 Publication
    Mutagenesisi171 – 1711S → A: Strong decrease in activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 668668DNA damage-responsive serine/threonine-protein kinase RqkAPRO_0000429795Add
    BLAST

    Post-translational modificationi

    Autophosphorylated.

    Keywords - PTMi

    Phosphoprotein

    Expressioni

    Inductioni

    Expression is induced in response to DNA damage.1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi243230.DR_2518.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9RRH3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini13 – 272260Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Domaini

    Contains an N-terminal catalytic kinase domain and a C-terminal PQQ binding domain.1 Publication

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    HOGENOMiHOG000099391.
    OMAiPFEGEND.
    OrthoDBiEOG6B35XT.

    Family and domain databases

    Gene3Di2.140.10.10. 1 hit.
    InterProiIPR011009. Kinase-like_dom.
    IPR018391. PQQ_beta_propeller_repeat.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR027295. Quinonprotein_ADH-like_fam.
    IPR011047. Quinonprotein_ADH-like_supfam.
    IPR008266. Tyr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00564. PQQ. 7 hits.
    [Graphical view]
    SUPFAMiSSF50998. SSF50998. 2 hits.
    SSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9RRH3-1 [UniParc]FASTAAdd to Basket

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    MPLTPGTLLA GRYELLALLG EGGSAQVYRA QDGLLGREVA LKVMHDYLPE    50
    SDRSRFLREV RTLARLTHPG VVPVLDLGQE PEAGRPFFTM PLLTGGPITR 100
    LGPLEDAPGP LARFLTAAAF ASRALHHVHS HGIVHRDLTP GNVLLDDTGL 150
    PRIMDFGLVA LSEQTRHLTR SGVTLGTPAY MAPEQAKGGG VDARSDLYAL 200
    GAVLYRVACG SPPFVGDSDQ SVLYQHVYEP VPDPRDLNPA VPDAVARVLL 250
    WLLAKRADRR PQSGAALAHL WALARRDLWT THARGQYRGG RARTGEHPDG 300
    PARVSDMQEL WSVALPGEVT WPAAVVGEGD LVAVGTRGGQ LVLTHTSGRP 350
    FATYAARDEV TAPATLIGGH VLYGAWDGTL RRVELQSGSE VWRHQARAEF 400
    TGAPTVWGGR LLAPSRDGHL HALSLRTGEL AWAYRAGGSL AASPLVWAGA 450
    ALQCDETGWL HALDARSGTP LWKVEVGTVH ATPALLPGPP GTATLVIATW 500
    EGEVHAIGLE VQNGRAALAG EDAIRWTYDV EDEVWASPAL TALDLPPDSG 550
    AAPDASAAPG GVVVVAGWGG KVRGLRLADG EDLWERTLDG RVTASPVISA 600
    GLVFLATEGG ELLALDVRNG EVRWTCRERS GVQATPLAAS GTLYVAFMDG 650
    TLRAYRNAHP EWRSEQEG 668
    Length:668
    Mass (Da):71,126
    Last modified:May 1, 2000 - v1
    Checksum:i22997D8E4127BC92
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000513 Genomic DNA. Translation: AAF12057.1.
    PIRiC75264.
    RefSeqiNP_296238.1. NC_001263.1.

    Genome annotation databases

    EnsemblBacteriaiAAF12057; AAF12057; DR_2518.
    GeneIDi1798368.
    KEGGidra:DR_2518.
    PATRICi21632771. VBIDeiRad64572_2760.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE000513 Genomic DNA. Translation: AAF12057.1 .
    PIRi C75264.
    RefSeqi NP_296238.1. NC_001263.1.

    3D structure databases

    ProteinModelPortali Q9RRH3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243230.DR_2518.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAF12057 ; AAF12057 ; DR_2518 .
    GeneIDi 1798368.
    KEGGi dra:DR_2518.
    PATRICi 21632771. VBIDeiRad64572_2760.

    Phylogenomic databases

    HOGENOMi HOG000099391.
    OMAi PFEGEND.
    OrthoDBi EOG6B35XT.

    Enzyme and pathway databases

    BioCyci DRAD243230:GH46-2570-MONOMER.

    Family and domain databases

    Gene3Di 2.140.10.10. 1 hit.
    InterProi IPR011009. Kinase-like_dom.
    IPR018391. PQQ_beta_propeller_repeat.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR027295. Quinonprotein_ADH-like_fam.
    IPR011047. Quinonprotein_ADH-like_supfam.
    IPR008266. Tyr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00564. PQQ. 7 hits.
    [Graphical view ]
    SUPFAMi SSF50998. SSF50998. 2 hits.
    SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422.
    2. "Characterization of a DNA damage-inducible membrane protein kinase from Deinococcus radiodurans and its role in bacterial radioresistance and DNA strand break repair."
      Rajpurohit Y.S., Misra H.S.
      Mol. Microbiol. 77:1470-1482(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, COFACTOR, ENZYME REGULATION, INDUCTION, DOMAIN, AUTOPHOSPHORYLATION, DISRUPTION PHENOTYPE.
      Strain: ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422.
    3. "Structure-function study of deinococcal serine/threonine protein kinase implicates its kinase activity and DNA repair protein phosphorylation roles in radioresistance of Deinococcus radiodurans."
      Rajpurohit Y.S., Misra H.S.
      Int. J. Biochem. Cell Biol. 45:2541-2552(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, GENE NAME, MUTAGENESIS OF LYS-42; SER-162; THR-169 AND SER-171.
      Strain: ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422.
    4. "Pyrroloquinoline quinone and a quinoprotein kinase support gamma-radiation resistance in Deinococcus radiodurans and regulate gene expression."
      Rajpurohit Y.S., Desai S.S., Misra H.S.
      J. Basic Microbiol. 53:518-531(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN REGULATION OF GENES, DISRUPTION PHENOTYPE.
      Strain: ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422.

    Entry informationi

    Entry nameiRQKA_DEIRA
    AccessioniPrimary (citable) accession number: Q9RRH3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 9, 2014
    Last sequence update: May 1, 2000
    Last modified: October 1, 2014
    This is version 114 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3