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Q9RRH3 (RQKA_DEIRA) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA damage-responsive serine/threonine-protein kinase RqkA

EC=2.7.11.1
Alternative name(s):
Radiation and pyrroloquinoline quinone inducible protein kinase
Gene names
Name:rqkA
Ordered Locus Names:DR_2518
OrganismDeinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422) [Reference proteome] [HAMAP]
Taxonomic identifier243230 [NCBI]
Taxonomic lineageBacteriaDeinococcus-ThermusDeinococciDeinococcalesDeinococcaceaeDeinococcus

Protein attributes

Sequence length668 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays an important role in radiation resistance and DNA double-strand break (DSB) repair. Involved in transcriptional regulation of genes important for bacterial stress response. Phosphorylates PprA in vitro. Ref.2 Ref.3 Ref.4

Catalytic activity

ATP + a protein = ADP + a phosphoprotein. Ref.2 Ref.3

Cofactor

PQQ. Ref.2

Enzyme regulation

Autokinase activity is stimulated by DNA damage. Stimulated by PQQ and DNA ends in vitro. Ref.2

Induction

Expression is induced in response to DNA damage. Ref.2

Domain

Contains an N-terminal catalytic kinase domain and a C-terminal PQQ binding domain. Ref.2

Post-translational modification

Autophosphorylated. Ref.2

Disruption phenotype

Mutant exhibits higher sensitivity to different DNA-damaging agents. It shows altered phosphoprotein profile and impaired DSB repair. Deletion affects expression of genes involved in intermediary metabolism, stress response and growth under stressed conditions. Ref.2 Ref.4

Sequence similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 668668DNA damage-responsive serine/threonine-protein kinase RqkA
PRO_0000429795

Regions

Domain13 – 272260Protein kinase
Nucleotide binding19 – 279ATP By similarity

Sites

Active site1371Proton acceptor By similarity
Binding site421ATP Probable

Experimental info

Mutagenesis421K → A: Lack of activity. Ref.3
Mutagenesis1621S → A: Almost no change in activity. Ref.3
Mutagenesis1691T → A: Strong decrease in activity. Ref.3
Mutagenesis1711S → A: Strong decrease in activity. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q9RRH3 [UniParc].

Last modified May 1, 2000. Version 1.
Checksum: 22997D8E4127BC92

FASTA66871,126
        10         20         30         40         50         60 
MPLTPGTLLA GRYELLALLG EGGSAQVYRA QDGLLGREVA LKVMHDYLPE SDRSRFLREV 

        70         80         90        100        110        120 
RTLARLTHPG VVPVLDLGQE PEAGRPFFTM PLLTGGPITR LGPLEDAPGP LARFLTAAAF 

       130        140        150        160        170        180 
ASRALHHVHS HGIVHRDLTP GNVLLDDTGL PRIMDFGLVA LSEQTRHLTR SGVTLGTPAY 

       190        200        210        220        230        240 
MAPEQAKGGG VDARSDLYAL GAVLYRVACG SPPFVGDSDQ SVLYQHVYEP VPDPRDLNPA 

       250        260        270        280        290        300 
VPDAVARVLL WLLAKRADRR PQSGAALAHL WALARRDLWT THARGQYRGG RARTGEHPDG 

       310        320        330        340        350        360 
PARVSDMQEL WSVALPGEVT WPAAVVGEGD LVAVGTRGGQ LVLTHTSGRP FATYAARDEV 

       370        380        390        400        410        420 
TAPATLIGGH VLYGAWDGTL RRVELQSGSE VWRHQARAEF TGAPTVWGGR LLAPSRDGHL 

       430        440        450        460        470        480 
HALSLRTGEL AWAYRAGGSL AASPLVWAGA ALQCDETGWL HALDARSGTP LWKVEVGTVH 

       490        500        510        520        530        540 
ATPALLPGPP GTATLVIATW EGEVHAIGLE VQNGRAALAG EDAIRWTYDV EDEVWASPAL 

       550        560        570        580        590        600 
TALDLPPDSG AAPDASAAPG GVVVVAGWGG KVRGLRLADG EDLWERTLDG RVTASPVISA 

       610        620        630        640        650        660 
GLVFLATEGG ELLALDVRNG EVRWTCRERS GVQATPLAAS GTLYVAFMDG TLRAYRNAHP 


EWRSEQEG 

« Hide

References

« Hide 'large scale' references
[1]"Genome sequence of the radioresistant bacterium Deinococcus radiodurans R1."
White O., Eisen J.A., Heidelberg J.F., Hickey E.K., Peterson J.D., Dodson R.J., Haft D.H., Gwinn M.L., Nelson W.C., Richardson D.L., Moffat K.S., Qin H., Jiang L., Pamphile W., Crosby M., Shen M., Vamathevan J.J., Lam P. expand/collapse author list , McDonald L.A., Utterback T.R., Zalewski C., Makarova K.S., Aravind L., Daly M.J., Minton K.W., Fleischmann R.D., Ketchum K.A., Nelson K.E., Salzberg S.L., Smith H.O., Venter J.C., Fraser C.M.
Science 286:1571-1577(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422.
[2]"Characterization of a DNA damage-inducible membrane protein kinase from Deinococcus radiodurans and its role in bacterial radioresistance and DNA strand break repair."
Rajpurohit Y.S., Misra H.S.
Mol. Microbiol. 77:1470-1482(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY, COFACTOR, ENZYME REGULATION, INDUCTION, DOMAIN, AUTOPHOSPHORYLATION, DISRUPTION PHENOTYPE.
Strain: ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422.
[3]"Structure-function study of deinococcal serine/threonine protein kinase implicates its kinase activity and DNA repair protein phosphorylation roles in radioresistance of Deinococcus radiodurans."
Rajpurohit Y.S., Misra H.S.
Int. J. Biochem. Cell Biol. 45:2541-2552(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY, GENE NAME, MUTAGENESIS OF LYS-42; SER-162; THR-169 AND SER-171.
Strain: ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422.
[4]"Pyrroloquinoline quinone and a quinoprotein kinase support gamma-radiation resistance in Deinococcus radiodurans and regulate gene expression."
Rajpurohit Y.S., Desai S.S., Misra H.S.
J. Basic Microbiol. 53:518-531(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN REGULATION OF GENES, DISRUPTION PHENOTYPE.
Strain: ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE000513 Genomic DNA. Translation: AAF12057.1.
PIRC75264.
RefSeqNP_296238.1. NC_001263.1.

3D structure databases

ProteinModelPortalQ9RRH3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING243230.DR_2518.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAF12057; AAF12057; DR_2518.
GeneID1798368.
KEGGdra:DR_2518.
PATRIC21632771. VBIDeiRad64572_2760.

Phylogenomic databases

HOGENOMHOG000099391.
OMAPFEGEND.
OrthoDBEOG6B35XT.

Enzyme and pathway databases

BioCycDRAD243230:GH46-2570-MONOMER.

Family and domain databases

Gene3D2.140.10.10. 1 hit.
InterProIPR011009. Kinase-like_dom.
IPR018391. PQQ_beta_propeller_repeat.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR027295. Quinonprotein_ADH-like_fam.
IPR011047. Quinonprotein_ADH-like_supfam.
IPR008266. Tyr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00564. PQQ. 7 hits.
[Graphical view]
SUPFAMSSF50998. SSF50998. 2 hits.
SSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRQKA_DEIRA
AccessionPrimary (citable) accession number: Q9RRH3
Entry history
Integrated into UniProtKB/Swiss-Prot: July 9, 2014
Last sequence update: May 1, 2000
Last modified: July 9, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families