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Protein

Fumarate hydratase class II

Gene

fumC

Organism
Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the TCA cycle. Catalyzes the stereospecific interconversion of fumarate to L-malate.UniRule annotation

Miscellaneous

There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors.UniRule annotation

Catalytic activityi

(S)-malate = fumarate + H2O.UniRule annotation

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from fumarate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Fumarate hydratase class II (fumC)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from fumarate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei189SubstrateUniRule annotation1
Active sitei190Proton donor/acceptorUniRule annotation1
Active sitei320UniRule annotation1
Binding sitei321SubstrateUniRule annotation1
Sitei333Important for catalytic activityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processTricarboxylic acid cycle

Enzyme and pathway databases

UniPathwayiUPA00223; UER01007.

Names & Taxonomyi

Protein namesi
Recommended name:
Fumarate hydratase class IIUniRule annotation (EC:4.2.1.2UniRule annotation)
Short name:
Fumarase CUniRule annotation
Alternative name(s):
Aerobic fumaraseUniRule annotation
Iron-independent fumaraseUniRule annotation
Gene namesi
Name:fumCUniRule annotation
Ordered Locus Names:DR_2627
OrganismiDeinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)
Taxonomic identifieri243230 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciDeinococcalesDeinococcaceaeDeinococcus
Proteomesi
  • UP000002524 Componenti: Chromosome I

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001612741 – 464Fumarate hydratase class IIAdd BLAST464

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi243230.DR_2627.

Structurei

3D structure databases

ProteinModelPortaliQ9RR70.
SMRiQ9RR70.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni100 – 102Substrate bindingUniRule annotation3
Regioni131 – 134Substrate binding (B site)UniRule annotation4
Regioni141 – 143Substrate bindingUniRule annotation3
Regioni326 – 328Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C9Q. Bacteria.
COG0114. LUCA.
HOGENOMiHOG000061736.
InParanoidiQ9RR70.
KOiK01679.
OMAiVSFTDNC.
OrthoDBiPOG091H01XG.

Family and domain databases

CDDicd01362. Fumarase_classII. 1 hit.
Gene3Di1.10.275.10. 1 hit.
HAMAPiMF_00743. FumaraseC. 1 hit.
InterProiView protein in InterPro
IPR005677. Fum_hydII.
IPR024083. Fumarase/histidase_N.
IPR018951. Fumarase_C_C.
IPR020557. Fumarate_lyase_CS.
IPR000362. Fumarate_lyase_fam.
IPR022761. Fumarate_lyase_N.
IPR008948. L-Aspartase-like.
PANTHERiPTHR11444. PTHR11444. 1 hit.
PfamiView protein in Pfam
PF10415. FumaraseC_C. 1 hit.
PF00206. Lyase_1. 1 hit.
PRINTSiPR00149. FUMRATELYASE.
SUPFAMiSSF48557. SSF48557. 1 hit.
TIGRFAMsiTIGR00979. fumC_II. 1 hit.
PROSITEiView protein in PROSITE
PS00163. FUMARATE_LYASES. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9RR70-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKTRQETDT MGKMDVDASR YWGAQTERSI HNFPIGRDTF VWGRPVIRAL
60 70 80 90 100
GILKKGAAQA NAELGELPAD VADLIVKAAD EVIAGKLDEH FPLVVFQTGS
110 120 130 140 150
GTQSNMNANE VISNRAIELA GGEMGSKKPV HPNDHVNRGQ SSNDTFPTAM
160 170 180 190 200
HIAVVLELNE RLYGAVGKLR DTLHAKAEQY KDLVKVGRTH LQDATPITLG
210 220 230 240 250
QEIGGWVAQL DYALSEVKHA GEGLLDLAIG GTAVGTGLNA HPKFGDLAAK
260 270 280 290 300
KYEEETGYHF RSAENKFAAL SAHDALVQTS AALRTLAGAL MKMANDVRWL
310 320 330 340 350
ASGPRNGIGE ITIPENEPGS SIMPGKVNPT QSEAMTMVAT RVFGNDATVA
360 370 380 390 400
FAGSQGNFQL NVFKPVMVHA VLESIRLISD ACLAFNDHCA VGIQPNEAKI
410 420 430 440 450
KENLDKNLMQ VTALNRHIGY DKAAAIAKKA HKEGTSLKDA ALALGYVTED
460
EFAQWVVPLG MTHN
Length:464
Mass (Da):49,876
Last modified:May 1, 2000 - v1
Checksum:i6CE8DC9A8D90A07E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000513 Genomic DNA. Translation: AAF12164.1.
PIRiB75250.
RefSeqiNP_296346.1. NC_001263.1.
WP_010889251.1. NZ_CP015081.1.

Genome annotation databases

EnsemblBacteriaiAAF12164; AAF12164; DR_2627.
GeneIDi1797634.
KEGGidra:DR_2627.
PATRICifig|243230.17.peg.2875.

Similar proteinsi

Entry informationi

Entry nameiFUMC_DEIRA
AccessioniPrimary (citable) accession number: Q9RR70
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: May 1, 2000
Last modified: August 30, 2017
This is version 108 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families