Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glycine betaine/carnitine transport ATP-binding protein GbuA

Gene

gbuA

Organism
Listeria monocytogenes serotype 1/2a (strain 10403S)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Part of the ABC transporter complex GbuABC involved in glycine betaine uptake. Responsible for energy coupling to the transport system. Involved, with BetL and OpuC, in osmoprotection and cryoprotection of Listeria. Can also uptake carnitine when carnitine is abundant in the growth medium.4 Publications

Catalytic activityi

ATP + H2O + quaternary amine(Out) = ADP + phosphate + quaternary amine(In).

Enzyme regulationi

The complex is activated by an osmotic gradient or by low temperature.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi60 – 678ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • amino acid transport Source: UniProtKB-KW
  • carnitine transmembrane transport Source: GOC
  • carnitine transport Source: UniProtKB
  • glycine betaine transport Source: UniProtKB
  • response to cold Source: UniProtKB
  • response to osmotic stress Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Amino-acid transport, Stress response, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciLMON169963:LMO1014-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycine betaine/carnitine transport ATP-binding protein GbuA (EC:3.6.3.32)
Gene namesi
Name:gbuA
Ordered Locus Names:LMRG_02114
OrganismiListeria monocytogenes serotype 1/2a (strain 10403S)
Taxonomic identifieri393133 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesListeriaceaeListeria
Proteomesi
  • UP000001288 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Mutants are impaired in the transport of glycine betaine at elevated osmolarity and at decreased temperature.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 397397Glycine betaine/carnitine transport ATP-binding protein GbuAPRO_0000417958Add
BLAST

Interactioni

Subunit structurei

The complex is composed of two ATP-binding proteins (GbuA), two transmembrane proteins (GbuB) and a solute-binding protein (GbuC).1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ9RR46.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini28 – 264237ABC transporterPROSITE-ProRule annotationAdd
BLAST
Domaini279 – 33557CBS 1PROSITE-ProRule annotationAdd
BLAST
Domaini340 – 39556CBS 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ABC transporter superfamily.Curated
Contains 1 ABC transporter domain.PROSITE-ProRule annotation
Contains 2 CBS domains.PROSITE-ProRule annotation

Keywords - Domaini

CBS domain, Repeat

Phylogenomic databases

KOiK02000.
OMAiPLIRLEM.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR000644. CBS_dom.
IPR005892. Gly-betaine_transp_ATP-bd.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00005. ABC_tran. 1 hit.
PF00571. CBS. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01186. proV. 1 hit.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
PS51371. CBS. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9RR46-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKIKVEELT KIFGKKASKA SSLLSQGKSK TDILKETGAT IGVNKASFSV
60 70 80 90 100
EEGEIFVIMG LSGSGKSTLV RLLNRLIEPT SGKIWLDGKE LSSLNKKELL
110 120 130 140 150
EVRRKSMSMV FQNFGLFPNR TINRNVEYGL EIQGMDKEER EKNAAESLAL
160 170 180 190 200
VGLAGYGDQY PSQLSGGMQQ RVGLARALAN NPDILLMDEA FSALDPLNRK
210 220 230 240 250
DMQDQLLDLQ DKMKKTIIFI THDLDEALRI GDHIMIMRDG SVVQTGSPEE
260 270 280 290 300
ILAHPANEYV EKFIEDVDRS KVYTASNVMI RPEIVNFEKD GPRVALKRMR
310 320 330 340 350
EAGTSSVFVV KRNRELVGIV HAAEVSKLVK ENITSLETAL HRDVPTTGLD
360 370 380 390
TPLAEIMDTI STTTIPIAVT EDGKLKGIII RGSVLAALSG NEVNVNA
Length:397
Mass (Da):43,626
Last modified:May 1, 2000 - v1
Checksum:i50D99F8625625434
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF039835 Genomic DNA. Translation: AAD29104.1.
CP002002 Genomic DNA. Translation: AEO06014.1.
PIRiAF1201.
RefSeqiWP_003722878.1. NC_017544.1.

Genome annotation databases

EnsemblBacteriaiAEO06014; AEO06014; LMRG_02114.
KEGGilmt:LMRG_02114.
PATRICi20311128. VBILisMon69206_1042.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF039835 Genomic DNA. Translation: AAD29104.1.
CP002002 Genomic DNA. Translation: AEO06014.1.
PIRiAF1201.
RefSeqiWP_003722878.1. NC_017544.1.

3D structure databases

ProteinModelPortaliQ9RR46.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAEO06014; AEO06014; LMRG_02114.
KEGGilmt:LMRG_02114.
PATRICi20311128. VBILisMon69206_1042.

Phylogenomic databases

KOiK02000.
OMAiPLIRLEM.

Enzyme and pathway databases

BioCyciLMON169963:LMO1014-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR000644. CBS_dom.
IPR005892. Gly-betaine_transp_ATP-bd.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00005. ABC_tran. 1 hit.
PF00571. CBS. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01186. proV. 1 hit.
PROSITEiPS00211. ABC_TRANSPORTER_1. 1 hit.
PS50893. ABC_TRANSPORTER_2. 1 hit.
PS51371. CBS. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of an ATP-driven, osmoregulated glycine betaine transport system in Listeria monocytogenes."
    Ko R., Smith L.T.
    Appl. Environ. Microbiol. 65:4040-4048(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBUNIT, DISRUPTION PHENOTYPE.
    Strain: 10403S.
  2. "The genome sequence of Listeria monocytogenes strain 10403S."
    The Broad Institute Genome Sequencing Platform, The Broad Institute Genome Sequencing Center for Infectious Disease
    Borowsky M., Borodovsky M., Young S.K., Zeng Q., Koehrsen M., Fitzgerald M., Wiedmann M., Swaminathan B., Lauer P., Portnoy D., Cossart P., Buchrieser C., Higgins D., Abouelleil A., Alvarado L., Arachchi H.M., Berlin A., Borenstein D.
    , Brown A., Chapman S.B., Chen Z., Dunbar C.D., Engels R., Freedman E., Gearin G., Gellesch M., Goldberg J., Griggs A., Gujja S., Heilman E., Heiman D., Howarth C., Jen D., Larson L., Lui A., MacDonald J., Mehta T., Montmayeur A., Neiman D., Park D., Pearson M., Priest M., Richards J., Roberts A., Saif S., Shea T., Shenoy N., Sisk P., Stolte C., Sykes S., Walk T., White J., Yandava C., Haas B., Nusbaum C., Birren B.
    Submitted (APR-2010) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 10403S.
  3. "Osmotic and chill activation of glycine betaine porter II in Listeria monocytogenes membrane vesicles."
    Gerhardt P.N., Tombras Smith L., Smith G.M.
    J. Bacteriol. 182:2544-2550(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ENZYME REGULATION.
  4. "Gbu glycine betaine porter and carnitine uptake in osmotically stressed Listeria monocytogenes cells."
    Mendum M.L., Smith L.T.
    Appl. Environ. Microbiol. 68:5647-5655(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN CARNITINE UPTAKE.
    Strain: 10403S.
  5. "Three transporters mediate uptake of glycine betaine and carnitine by Listeria monocytogenes in response to hyperosmotic stress."
    Angelidis A.S., Smith G.M.
    Appl. Environ. Microbiol. 69:1013-1022(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN GLYCINE BETAINE AND CARNITINE UPTAKE.
    Strain: 10403S.

Entry informationi

Entry nameiGBUA_LISM4
AccessioniPrimary (citable) accession number: Q9RR46
Secondary accession number(s): G2K536
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 13, 2012
Last sequence update: May 1, 2000
Last modified: May 11, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.