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Protein

Poly-beta-1,6-N-acetyl-D-glucosamine N-deacetylase

Gene

icaB

Organism
Staphylococcus aureus (strain NCTC 8325)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the N-deacetylation of poly-beta-1,6-N-acetyl-D-glucosamine (PNAG, also referred to as PIA), a biofilm adhesin polysaccharide. N-deacetylation is crucial for attachment of the polysaccharide to the bacterial cell surface; it leads to the introduction of positive charges in the otherwise neutral PIA polymer, allowing electrostatic interactions (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase

Enzyme and pathway databases

BioCyciSAUR93061:G1G5Y-2826-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Poly-beta-1,6-N-acetyl-D-glucosamine N-deacetylase (EC:3.5.1.-)
Short name:
PNAG N-deacetylase
Short name:
Poly-beta-1,6-GlcNAc N-deacetylase
Alternative name(s):
Biofilm polysaccharide intercellular adhesin deacetylase
Short name:
Biofilm PIA deacetylase
Intercellular adhesion protein B
Gene namesi
Name:icaB
Ordered Locus Names:SAOUHSC_03004
OrganismiStaphylococcus aureus (strain NCTC 8325)
Taxonomic identifieri93061 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi

Subcellular locationi

  • cell wall
  • Note: Attached to the cell surface.By similarity

GO - Cellular componenti

Keywords - Cellular componenti

Cell wall, Secreted

Pathology & Biotechi

Disruption phenotypei

Deletion of the icaADBCR genes leads to the inability to form biofilms, produce PIA or mediate N-acetylglucosaminyltransferase activity in vitro.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 28Sequence analysisAdd BLAST28
ChainiPRO_000002483329 – 290Poly-beta-1,6-N-acetyl-D-glucosamine N-deacetylaseAdd BLAST262

Interactioni

Protein-protein interaction databases

STRINGi93061.SAOUHSC_03004

Structurei

3D structure databases

ProteinModelPortaliQ9RQP7
SMRiQ9RQP7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini114 – 290NodB homologyPROSITE-ProRule annotationAdd BLAST177

Sequence similaritiesi

Belongs to the polysaccharide deacetylase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107UVV Bacteria
COG0726 LUCA
HOGENOMiHOG000280325
KOiK21478
OMAiHTNDLHY

Family and domain databases

InterProiView protein in InterPro
IPR011330 Glyco_hydro/deAcase_b/a-brl
IPR002509 NODB_dom
IPR023872 PNAG_deacetylase
PfamiView protein in Pfam
PF01522 Polysacc_deac_1, 1 hit
SUPFAMiSSF88713 SSF88713, 1 hit
TIGRFAMsiTIGR03933 PIA_icaB, 1 hit
PROSITEiView protein in PROSITE
PS51677 NODB, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9RQP7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKYRKFIILV LSILIILPVS TLDGHHIANA DDDSPKKLKY KENSALALNY
60 70 80 90 100
HRVRKANFLN NFIYFFSSSK EIKNYSVSQS QFESQIKWLK SHDAKFLTLK
110 120 130 140 150
EFLYYKKKGK FPKRSVWINF DDMDETIYEN AYPILKKYKI PATGFIITGH
160 170 180 190 200
VGEENFHNLD MISKKELKEM YKTGLWEFET HTHDLHNLSK NNKSKLMKAS
210 220 230 240 250
EATIIKDLNK SEKYLTKNFK KSQKTIAYPY GLMNDDKLPV IKKAGLKYGF
260 270 280 290
SLEEKAVTPN SNDYYIPRIL ISDDAFEHLI KRWDGFHEKD
Length:290
Mass (Da):34,105
Last modified:May 29, 2007 - v2
Checksum:i76D1B0DA47C726A8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti116V → E in AAD52057 (PubMed:10496925).Curated1
Sequence conflicti118I → V in AAD52057 (PubMed:10496925).Curated1
Sequence conflicti153E → G in AAD52057 (PubMed:10496925).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF086783 Genomic DNA Translation: AAD52057.1
CP000253 Genomic DNA Translation: ABD31991.1
RefSeqiWP_000877317.1, NC_007795.1
YP_501453.1, NC_007795.1

Genome annotation databases

EnsemblBacteriaiABD31991; ABD31991; SAOUHSC_03004
GeneIDi3921486
KEGGisao:SAOUHSC_03004
PATRICifig|93061.5.peg.2711

Entry informationi

Entry nameiICAB_STAA8
AccessioniPrimary (citable) accession number: Q9RQP7
Secondary accession number(s): Q2FUU7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: May 29, 2007
Last modified: February 28, 2018
This is version 78 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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