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Protein

Poly-beta-1,6-N-acetyl-D-glucosamine N-deacetylase

Gene

icaB

Organism
Staphylococcus aureus (strain NCTC 8325)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the N-deacetylation of poly-beta-1,6-N-acetyl-D-glucosamine (PNAG, also referred to as PIA), a biofilm adhesin polysaccharide. N-deacetylation is crucial for attachment of the polysaccharide to the bacterial cell surface; it leads to the introduction of positive charges in the otherwise neutral PIA polymer, allowing electrostatic interactions (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Poly-beta-1,6-N-acetyl-D-glucosamine N-deacetylase (EC:3.5.1.-)
Short name:
PNAG N-deacetylase
Short name:
Poly-beta-1,6-GlcNAc N-deacetylase
Alternative name(s):
Biofilm polysaccharide intercellular adhesin deacetylase
Short name:
Biofilm PIA deacetylase
Intercellular adhesion protein B
Gene namesi
Name:icaB
Ordered Locus Names:SAOUHSC_03004
OrganismiStaphylococcus aureus (strain NCTC 8325)
Taxonomic identifieri93061 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000008816 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Secreted

Pathology & Biotechi

Disruption phenotypei

Deletion of the icaADBCR genes leads to the inability to form biofilms, produce PIA or mediate N-acetylglucosaminyltransferase activity in vitro.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 28Sequence analysisAdd BLAST28
ChainiPRO_000002483329 – 290Poly-beta-1,6-N-acetyl-D-glucosamine N-deacetylaseAdd BLAST262

Interactioni

Protein-protein interaction databases

STRINGi93061.SAOUHSC_03004.

Structurei

3D structure databases

ProteinModelPortaliQ9RQP7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini114 – 290NodB homologyPROSITE-ProRule annotationAdd BLAST177

Sequence similaritiesi

Belongs to the polysaccharide deacetylase family.Curated
Contains 1 NodB homology domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107UVV. Bacteria.
COG0726. LUCA.
HOGENOMiHOG000280325.
OMAiLYHRFGP.

Family and domain databases

Gene3Di3.20.20.370. 1 hit.
InterProiIPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR002509. NODB_dom.
IPR023872. PNAG_deacetylase.
[Graphical view]
PfamiPF01522. Polysacc_deac_1. 1 hit.
[Graphical view]
SUPFAMiSSF88713. SSF88713. 1 hit.
TIGRFAMsiTIGR03933. PIA_icaB. 1 hit.
PROSITEiPS51677. NODB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9RQP7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKYRKFIILV LSILIILPVS TLDGHHIANA DDDSPKKLKY KENSALALNY
60 70 80 90 100
HRVRKANFLN NFIYFFSSSK EIKNYSVSQS QFESQIKWLK SHDAKFLTLK
110 120 130 140 150
EFLYYKKKGK FPKRSVWINF DDMDETIYEN AYPILKKYKI PATGFIITGH
160 170 180 190 200
VGEENFHNLD MISKKELKEM YKTGLWEFET HTHDLHNLSK NNKSKLMKAS
210 220 230 240 250
EATIIKDLNK SEKYLTKNFK KSQKTIAYPY GLMNDDKLPV IKKAGLKYGF
260 270 280 290
SLEEKAVTPN SNDYYIPRIL ISDDAFEHLI KRWDGFHEKD
Length:290
Mass (Da):34,105
Last modified:May 29, 2007 - v2
Checksum:i76D1B0DA47C726A8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti116V → E in AAD52057 (PubMed:10496925).Curated1
Sequence conflicti118I → V in AAD52057 (PubMed:10496925).Curated1
Sequence conflicti153E → G in AAD52057 (PubMed:10496925).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF086783 Genomic DNA. Translation: AAD52057.1.
CP000253 Genomic DNA. Translation: ABD31991.1.
RefSeqiWP_000877317.1. NC_007795.1.
YP_501453.1. NC_007795.1.

Genome annotation databases

EnsemblBacteriaiABD31991; ABD31991; SAOUHSC_03004.
GeneIDi28379686.
3921486.
KEGGisao:SAOUHSC_03004.
PATRICi19583211. VBIStaAur99865_2711.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF086783 Genomic DNA. Translation: AAD52057.1.
CP000253 Genomic DNA. Translation: ABD31991.1.
RefSeqiWP_000877317.1. NC_007795.1.
YP_501453.1. NC_007795.1.

3D structure databases

ProteinModelPortaliQ9RQP7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi93061.SAOUHSC_03004.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABD31991; ABD31991; SAOUHSC_03004.
GeneIDi28379686.
3921486.
KEGGisao:SAOUHSC_03004.
PATRICi19583211. VBIStaAur99865_2711.

Phylogenomic databases

eggNOGiENOG4107UVV. Bacteria.
COG0726. LUCA.
HOGENOMiHOG000280325.
OMAiLYHRFGP.

Family and domain databases

Gene3Di3.20.20.370. 1 hit.
InterProiIPR011330. Glyco_hydro/deAcase_b/a-brl.
IPR002509. NODB_dom.
IPR023872. PNAG_deacetylase.
[Graphical view]
PfamiPF01522. Polysacc_deac_1. 1 hit.
[Graphical view]
SUPFAMiSSF88713. SSF88713. 1 hit.
TIGRFAMsiTIGR03933. PIA_icaB. 1 hit.
PROSITEiPS51677. NODB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiICAB_STAA8
AccessioniPrimary (citable) accession number: Q9RQP7
Secondary accession number(s): Q2FUU7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: May 29, 2007
Last modified: November 2, 2016
This is version 72 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.