Q9RQJ2 (PAD_PORGI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Peptidylarginine deiminase
EC=3.5.3.-

Gene names

Ordered Locus Names:

PG_1424

Organism

Porphyromonas gingivalis (strain ATCC BAA-308 / W83) [Complete proteome] [HAMAP]

Taxonomic identifier

242619 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Bacteroidetes/Chlorobi group › Bacteroidetes › Bacteroidia › Bacteroidales › Porphyromonadaceae › Porphyromonas › Porphyromonas gingivalis

Protein attributes

Sequence length	556 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Deiminates the guanidino group of C-terminal arginine residues on a variety of peptides, including the vasoregulatory peptide-hormone bradykinin, to yield ammonia and a citrulline residue. May promote the growth of the pathogen in the periodontal pocket by producing ammonia, ammonia having a protective effect during acidic cleaning cycles in the mouth. Ref.1
Cofactor	FAD or FMN Potential. Ref.1
Enzyme regulation	Inhibited by cysteine and TLCK. Inhibited by high concentration of thiourea and thio-L-citrulline. Ref.1
Subcellular location	Secreted Potential.
Sequence similarities	Belongs to the agmatine deiminase family.
Biophysicochemical properties	Kinetic parameters: KM=2.9 µM for Benzoyl-L-arginine Ref.1 KM=28 µM for Benzoylglycyl-L-arginine KM=152 µM for BAA KM=874 µM for BAEE KM=141 µM for L-arginine KM=47 µM for BK Vmax=104 nmol/min/mg enzyme with Benzoyl-L-arginine as substrate Vmax=186 nmol/min/mg enzyme with Benzoylglycyl-L-arginine as substrate Vmax=77 nmol/min/mg enzyme with BAA as substrate Vmax=90 nmol/min/mg enzyme with BAEE as substrate Vmax=26 nmol/min/mg enzyme with L-arginine as substrate Vmax=117 nmol/min/mg enzyme with BK as substrate pH dependence: Optimum pH is 9.3.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Ligand	FAD FMN
Molecular function	Hydrolase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological_process	putrescine biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	protein-arginine deiminase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Propeptide	24 – 43	20	Probable		PRO_0000001044
	Chain	44 – 556	513	Peptidylarginine deiminase		PRO_0000001045
Sites
	Active site	351	1	Amidino-cysteine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9RQJ2 [UniParc]. Last modified May 1, 2000. Version 1. Checksum: 6E189E508868DCF6	FASTA	556	61,730
	10 20 30 40 50 60 MKKLLQAKAL ILALGLFQLP AIAQTQMQAD RTNGQFATEE MQRAFQETNP PAGPVRAIAE 70 80 90 100 110 120 YERSAAVLVR YPFGIPMELI KELAKNDKVI TIVASESQKN TVITQYTQSG VNLSNCDFII 130 140 150 160 170 180 AKTDSYWTRD YTGWFAMYDT NKVGLVDFIY NRPRPNDDEF PKYEAQYLGI EMFGMKLKQT 190 200 210 220 230 240 GGNYMTDGYG SAVQSHIAYT ENSSLSQAQV NQKMKDYLGI THHDVVQDPN GEYINHVDCW 250 260 270 280 290 300 GKYLAPNKIL IRKVPDNHPQ HQALEDMAAY FAAQTCAWGT KYEVYRALAT NEQPYTNSLI 310 320 330 340 350 360 LNNRVFVPVN GPASVDNDAL NVYKTAMPGY EIIGVKGASG TPWLGTDALH CRTHEVADKG 370 380 390 400 410 420 YLYIKHYPIL GEQAGPDYKI EADVVSCANA TISPVQCYYR INGSGSFKAA DMTMESTGHY 430 440 450 460 470 480 TYSFTGLNKN DKVEYYISAA DNSGRKETYP FIGEPDPFKF TCMNETNTCT VTGAAKALRA 490 500 510 520 530 540 WFNAGRSELA VSVSLNIAGT YRIKLYNTAG EEVAAMTKEL VAGTSVFSMD VYSQAPGTYV 550 LVVEGNGIRE TMKILK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Purification, characterization, and sequence analysis of a potential virulence factor from Porphyromonas gingivalis, peptidylarginine deiminase." McGraw W.T., Potempa J., Farley D., Travis J. Infect. Immun. 67:3248-3256(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 44-73, FUNCTION, ENZYME ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, POSSIBLE COFACTOR. Strain: ATCC BAA-308 / W83.
[2]	"Complete genome sequence of the oral pathogenic bacterium Porphyromonas gingivalis strain W83." Nelson K.E., Fleischmann R.D., DeBoy R.T., Paulsen I.T., Fouts D.E., Eisen J.A., Daugherty S.C., Dodson R.J., Durkin A.S., Gwinn M.L., Haft D.H., Kolonay J.F., Nelson W.C., Mason T.M., Tallon L., Gray J., Granger D., Tettelin H. , Dong H., Galvin J.L., Duncan M.J., Dewhirst F.E., Fraser C.M. J. Bacteriol. 185:5591-5601(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-308 / W83.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF100668 Genomic DNA. Translation: AAF06719.1. AE015924 Genomic DNA. Translation: AAQ66478.1.
RefSeq	NP_905579.1. NC_002950.2.
3D structure databases
ProteinModelPortal	Q9RQJ2.
ModBase	Search...
Protein-protein interaction databases
STRING	242619.PG1424.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAQ66478; AAQ66478; PG_1424.
GeneID	2552184.
KEGG	pgi:PG1424.
PATRIC	22979880. VBIPorGin134034_1324.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2957.
OMA	HILQTEN.
ProtClustDB	CLSK647850.
Enzyme and pathway databases
BioCyc	PGIN242619:GHX8-1246-MONOMER.
Family and domain databases
InterPro	IPR007466. Peptidyl-Arg-deiminase_porph. [Graphical view]
Pfam	PF04371. PAD_porph. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PAD_PORGI

Accession

Primary (citable) accession number: Q9RQJ2
Secondary accession number(s): Q7BW72

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2005
Last sequence update:	May 1, 2000
Last modified:	May 1, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program